void showResults() {
   int count = rt.getCounter();
   // if (count==0) return;
   boolean lastSlice = !processStack || slice == imp.getStackSize();
   if ((showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS) && slice == 1 && count > 0)
     imp.setOverlay(overlay);
   else if (outlines != null && lastSlice) {
     String title = imp != null ? imp.getTitle() : "Outlines";
     String prefix;
     if (showChoice == MASKS) prefix = "Mask of ";
     else if (showChoice == ROI_MASKS) prefix = "Count Masks of ";
     else prefix = "Drawing of ";
     outlines.update(drawIP);
     outputImage = new ImagePlus(prefix + title, outlines);
     if (inSituShow) {
       if (imp.getStackSize() == 1) Undo.setup(Undo.TRANSFORM, imp);
       imp.setStack(null, outputImage.getStack());
     } else if (!hideOutputImage) outputImage.show();
   }
   if (showResults && !processStack) {
     TextPanel tp = IJ.getTextPanel();
     if (beginningCount > 0 && tp != null && tp.getLineCount() != count) rt.show("Results");
     Analyzer.firstParticle = beginningCount;
     Analyzer.lastParticle = Analyzer.getCounter() - 1;
   } else Analyzer.firstParticle = Analyzer.lastParticle = 0;
 }
Пример #2
0
  public boolean beadCalibration3d() {
    imp = IJ.getImage();
    if (imp == null) {
      IJ.noImage();
      return false;
    } else if (imp.getStackSize() == 1) {
      IJ.error("Stack required");
      return false;
    } else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
      // In order to support 32bit images, pict[] must be changed to float[], and  getPixel(x, y);
      // requires a Float.intBitsToFloat() conversion
      IJ.error("8 or 16 bit greyscale image required");
      return false;
    }
    width = imp.getWidth();
    height = imp.getHeight();
    nslices = imp.getStackSize();
    imtitle = imp.getTitle();

    models[0] = "*None*";
    models[1] = "line";
    models[2] = "2nd degree polynomial";
    models[3] = "3rd degree polynomial";
    models[4] = "4th degree polynomial";

    GenericDialog gd = new GenericDialog("3D PALM calibration");
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
    gd.addNumericField(
        "Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
    gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
    gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
    gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
    gd.addCheckbox(
        "Show divergence of bead positions against model",
        prefs.get("QuickPALM.3Dcal_showDivergence", false));
    gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
    gd.addMessage("\n\nDon't forget to save the table in the end...");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.pthrsh", pthrsh * 100);
    cal_z = gd.getNextNumber();
    prefs.set("QuickPALM.z-step", cal_z);
    window = (int) gd.getNextNumber();
    prefs.set("QuickPALM.window", window);
    model = gd.getNextChoice();
    prefs.set("QuickPALM.model", model);
    part_divergence = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
    part_extrainfo = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
    return true;
  }
 public void run(ImageProcessor ip) {
   if (canceled) return;
   slice++;
   if (imp.getStackSize() > 1 && processStack) imp.setSlice(slice);
   if (imp.getType() == ImagePlus.COLOR_RGB) {
     ip = (ImageProcessor) imp.getProperty("Mask");
     ip.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
   }
   if (!analyze(imp, ip)) canceled = true;
   if (slice == imp.getStackSize()) {
     imp.updateAndDraw();
     if (saveRoi != null) imp.setRoi(saveRoi);
   }
 }
 void updateSliceSummary() {
   int slices = imp.getStackSize();
   float[] areas = rt.getColumn(ResultsTable.AREA);
   if (areas == null) areas = new float[0];
   String label = imp.getTitle();
   if (slices > 1) {
     label = imp.getStack().getShortSliceLabel(slice);
     label = label != null && !label.equals("") ? label : "" + slice;
   }
   String aLine = null;
   double sum = 0.0;
   int start = areas.length - particleCount;
   if (start < 0) return;
   for (int i = start; i < areas.length; i++) sum += areas[i];
   int places = Analyzer.getPrecision();
   Calibration cal = imp.getCalibration();
   String total = "\t" + ResultsTable.d2s(sum, places);
   String average = "\t" + ResultsTable.d2s(sum / particleCount, places);
   String fraction = "\t" + ResultsTable.d2s(sum * 100.0 / totalArea, 1);
   aLine = label + "\t" + particleCount + total + average + fraction;
   aLine = addMeans(aLine, areas.length > 0 ? start : -1);
   if (slices == 1) {
     Frame frame = WindowManager.getFrame("Summary");
     if (frame != null && (frame instanceof TextWindow) && summaryHdr.equals(prevHdr))
       tw = (TextWindow) frame;
   }
   if (tw == null) {
     String title = slices == 1 ? "Summary" : "Summary of " + imp.getTitle();
     tw = new TextWindow(title, summaryHdr, aLine, 450, 300);
     prevHdr = summaryHdr;
   } else tw.append(aLine);
 }
 /**
  * Saves statistics for one particle in a results table. This is a method subclasses may want to
  * override.
  */
 protected void saveResults(ImageStatistics stats, Roi roi) {
   analyzer.saveResults(stats, roi);
   if (recordStarts) {
     rt.addValue("XStart", stats.xstart);
     rt.addValue("YStart", stats.ystart);
   }
   if (addToManager) {
     if (roiManager == null) {
       if (Macro.getOptions() != null && Interpreter.isBatchMode())
         roiManager = Interpreter.getBatchModeRoiManager();
       if (roiManager == null) {
         Frame frame = WindowManager.getFrame("ROI Manager");
         if (frame == null) IJ.run("ROI Manager...");
         frame = WindowManager.getFrame("ROI Manager");
         if (frame == null || !(frame instanceof RoiManager)) {
           addToManager = false;
           return;
         }
         roiManager = (RoiManager) frame;
       }
       if (resetCounter) roiManager.runCommand("reset");
     }
     if (imp.getStackSize() > 1) roi.setPosition(imp.getCurrentSlice());
     if (lineWidth != 1) roi.setStrokeWidth(lineWidth);
     roiManager.add(imp, roi, rt.getCounter());
   }
   if (showResults) rt.addResults();
 }
Пример #6
0
 void scale(ImageProcessor ip) {
   if (newWindow) {
     Rectangle r = ip.getRoi();
     ImagePlus imp2 = imp.createImagePlus();
     imp2.setProcessor(title, ip.resize(newWidth, newHeight));
     Calibration cal = imp2.getCalibration();
     if (cal.scaled()) {
       cal.pixelWidth *= 1.0 / xscale;
       cal.pixelHeight *= 1.0 / yscale;
     }
     imp2.show();
     imp.trimProcessor();
     imp2.trimProcessor();
     imp2.changes = true;
   } else {
     if (processStack && imp.getStackSize() > 1) {
       Undo.reset();
       StackProcessor sp = new StackProcessor(imp.getStack(), ip);
       sp.scale(xscale, yscale, bgValue);
     } else {
       ip.snapshot();
       Undo.setup(Undo.FILTER, imp);
       ip.setSnapshotCopyMode(true);
       ip.scale(xscale, yscale);
       ip.setSnapshotCopyMode(false);
     }
     imp.killRoi();
     imp.updateAndDraw();
     imp.changes = true;
   }
 }
Пример #7
0
 void drawAllROIs(Graphics g) {
   RoiManager rm = RoiManager.getInstance();
   if (rm == null) {
     rm = Interpreter.getBatchModeRoiManager();
     if (rm != null && rm.getList().getItemCount() == 0) rm = null;
   }
   if (rm == null) {
     // if (showAllList!=null)
     //	overlay = showAllList;
     showAllROIs = false;
     repaint();
     return;
   }
   initGraphics(g, null, showAllColor);
   Hashtable rois = rm.getROIs();
   java.awt.List list = rm.getList();
   boolean drawLabels = rm.getDrawLabels();
   currentRoi = null;
   int n = list.getItemCount();
   if (IJ.debugMode) IJ.log("paint: drawing " + n + " \"Show All\" ROIs");
   if (labelRects == null || labelRects.length != n) labelRects = new Rectangle[n];
   if (!drawLabels) showAllList = new Overlay();
   else showAllList = null;
   if (imp == null) return;
   int currentImage = imp.getCurrentSlice();
   int channel = 0, slice = 0, frame = 0;
   boolean hyperstack = imp.isHyperStack();
   if (hyperstack) {
     channel = imp.getChannel();
     slice = imp.getSlice();
     frame = imp.getFrame();
   }
   drawNames = Prefs.useNamesAsLabels;
   for (int i = 0; i < n; i++) {
     String label = list.getItem(i);
     Roi roi = (Roi) rois.get(label);
     if (roi == null) continue;
     if (showAllList != null) showAllList.add(roi);
     if (i < 200 && drawLabels && roi == imp.getRoi()) currentRoi = roi;
     if (Prefs.showAllSliceOnly && imp.getStackSize() > 1) {
       if (hyperstack && roi.getPosition() == 0) {
         int c = roi.getCPosition();
         int z = roi.getZPosition();
         int t = roi.getTPosition();
         if ((c == 0 || c == channel) && (z == 0 || z == slice) && (t == 0 || t == frame))
           drawRoi(g, roi, drawLabels ? i : -1);
       } else {
         int position = roi.getPosition();
         if (position == 0) position = getSliceNumber(roi.getName());
         if (position == 0 || position == currentImage) drawRoi(g, roi, drawLabels ? i : -1);
       }
     } else drawRoi(g, roi, drawLabels ? i : -1);
   }
   ((Graphics2D) g).setStroke(Roi.onePixelWide);
   drawNames = false;
 }
Пример #8
0
 public String getImageInfo(ImagePlus imp, ImageProcessor ip) {
   String infoProperty = null;
   if (imp.getStackSize() > 1) {
     ImageStack stack = imp.getStack();
     String label = stack.getSliceLabel(imp.getCurrentSlice());
     if (label != null && label.indexOf('\n') > 0) infoProperty = label;
   }
   if (infoProperty == null) {
     infoProperty = (String) imp.getProperty("Info");
     if (infoProperty == null) infoProperty = getExifData(imp);
   }
   String info = getInfo(imp, ip);
   if (infoProperty != null) return infoProperty + "\n------------------------\n" + info;
   else return info;
 }
Пример #9
0
 void createNewStack(ImagePlus imp, ImageProcessor ip) {
   int nSlices = imp.getStackSize();
   int w = imp.getWidth(), h = imp.getHeight();
   ImagePlus imp2 = imp.createImagePlus();
   Rectangle r = ip.getRoi();
   boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight();
   ImageStack stack1 = imp.getStack();
   ImageStack stack2 = new ImageStack(newWidth, newHeight);
   ImageProcessor ip1, ip2;
   int method = interpolationMethod;
   if (w == 1 || h == 1) method = ImageProcessor.NONE;
   for (int i = 1; i <= nSlices; i++) {
     IJ.showStatus("Scale: " + i + "/" + nSlices);
     ip1 = stack1.getProcessor(i);
     String label = stack1.getSliceLabel(i);
     if (crop) {
       ip1.setRoi(r);
       ip1 = ip1.crop();
     }
     ip1.setInterpolationMethod(method);
     ip2 = ip1.resize(newWidth, newHeight, averageWhenDownsizing);
     if (ip2 != null) stack2.addSlice(label, ip2);
     IJ.showProgress(i, nSlices);
   }
   imp2.setStack(title, stack2);
   Calibration cal = imp2.getCalibration();
   if (cal.scaled()) {
     cal.pixelWidth *= 1.0 / xscale;
     cal.pixelHeight *= 1.0 / yscale;
   }
   IJ.showProgress(1.0);
   int[] dim = imp.getDimensions();
   imp2.setDimensions(dim[2], dim[3], dim[4]);
   if (imp.isComposite()) {
     imp2 = new CompositeImage(imp2, ((CompositeImage) imp).getMode());
     ((CompositeImage) imp2).copyLuts(imp);
   }
   if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true);
   if (newDepth > 0 && newDepth != oldDepth)
     imp2 = (new Resizer()).zScale(imp2, newDepth, interpolationMethod);
   if (imp2 != null) {
     imp2.show();
     imp2.changes = true;
   }
 }
Пример #10
0
 public void run(String arg) {
   imp = IJ.getImage();
   Roi roi = imp.getRoi();
   if (roi != null && !roi.isArea()) imp.killRoi(); // ignore any line selection
   ImageProcessor ip = imp.getProcessor();
   if (!showDialog(ip)) return;
   if (ip.getWidth() > 1 && ip.getHeight() > 1) ip.setInterpolate(interpolate);
   else ip.setInterpolate(false);
   ip.setBackgroundValue(bgValue);
   imp.startTiming();
   try {
     if (newWindow && imp.getStackSize() > 1 && processStack) createNewStack(imp, ip);
     else scale(ip);
   } catch (OutOfMemoryError o) {
     IJ.outOfMemory("Scale");
   }
   IJ.showProgress(1.0);
 }
Пример #11
0
 void createNewStack(ImagePlus imp, ImageProcessor ip) {
   Rectangle r = ip.getRoi();
   boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight();
   int nSlices = imp.getStackSize();
   ImageStack stack1 = imp.getStack();
   ImageStack stack2 = new ImageStack(newWidth, newHeight);
   ImageProcessor ip1, ip2;
   boolean interp = interpolate;
   if (imp.getWidth() == 1 || imp.getHeight() == 1) interp = false;
   for (int i = 1; i <= nSlices; i++) {
     IJ.showStatus("Scale: " + i + "/" + nSlices);
     ip1 = stack1.getProcessor(i);
     String label = stack1.getSliceLabel(i);
     if (crop) {
       ip1.setRoi(r);
       ip1 = ip1.crop();
     }
     ip1.setInterpolate(interp);
     ip2 = ip1.resize(newWidth, newHeight);
     if (ip2 != null) stack2.addSlice(label, ip2);
     IJ.showProgress(i, nSlices);
   }
   ImagePlus imp2 = imp.createImagePlus();
   imp2.setStack(title, stack2);
   Calibration cal = imp2.getCalibration();
   if (cal.scaled()) {
     cal.pixelWidth *= 1.0 / xscale;
     cal.pixelHeight *= 1.0 / yscale;
   }
   int[] dim = imp.getDimensions();
   imp2.setDimensions(dim[2], dim[3], dim[4]);
   IJ.showProgress(1.0);
   if (imp.isComposite()) {
     imp2 = new CompositeImage(imp2, 0);
     ((CompositeImage) imp2).copyLuts(imp);
   }
   if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true);
   imp2.show();
   imp2.changes = true;
 }
Пример #12
0
 void drawOverlay(Graphics g) {
   if (imp != null && imp.getHideOverlay()) return;
   Color labelColor = overlay.getLabelColor();
   if (labelColor == null) labelColor = Color.white;
   initGraphics(g, labelColor, Roi.getColor());
   int n = overlay.size();
   if (IJ.debugMode) IJ.log("paint: drawing " + n + " ROI display list");
   int currentImage = imp != null ? imp.getCurrentSlice() : -1;
   if (imp.getStackSize() == 1) currentImage = -1;
   int channel = 0, slice = 0, frame = 0;
   boolean hyperstack = imp.isHyperStack();
   if (hyperstack) {
     channel = imp.getChannel();
     slice = imp.getSlice();
     frame = imp.getFrame();
   }
   drawNames = overlay.getDrawNames();
   boolean drawLabels = drawNames || overlay.getDrawLabels();
   for (int i = 0; i < n; i++) {
     if (overlay == null) break;
     Roi roi = overlay.get(i);
     if (hyperstack && roi.getPosition() == 0) {
       int c = roi.getCPosition();
       int z = roi.getZPosition();
       int t = roi.getTPosition();
       if ((c == 0 || c == channel) && (z == 0 || z == slice) && (t == 0 || t == frame))
         drawRoi(g, roi, drawLabels ? i + LIST_OFFSET : -1);
     } else {
       int position = roi.getPosition();
       if (position == 0 || position == currentImage)
         drawRoi(g, roi, drawLabels ? i + LIST_OFFSET : -1);
     }
   }
   ((Graphics2D) g).setStroke(Roi.onePixelWide);
   drawNames = false;
 }
Пример #13
0
 boolean roiManagerSelect(int x, int y) {
   RoiManager rm = RoiManager.getInstance();
   if (rm == null) return false;
   Hashtable rois = rm.getROIs();
   java.awt.List list = rm.getList();
   int n = list.getItemCount();
   if (labelRects == null || labelRects.length != n) return false;
   boolean stackMode = imp != null && imp.getStackSize() > 1 && Prefs.showAllSliceOnly;
   for (int i = 0; i < n; i++) {
     if (labelRects[i] != null && labelRects[i].contains(x, y)) {
       if (stackMode) {
         int slice = getSliceNumber(list.getItem(i));
         if (slice != imp.getCurrentSlice()) continue;
       }
       // rm.select(i);
       // this needs to run on a separate thread, at least on OS X
       // "update2" does not clone the ROI so the "Show All"
       // outline moves as the user moves the RO.
       new ij.macro.MacroRunner("roiManager('select', " + i + "); roiManager('update2');");
       return true;
     }
   }
   return false;
 }
Пример #14
0
  String getInfo(ImagePlus imp, ImageProcessor ip) {
    String s = new String("\n");
    s += "Title: " + imp.getTitle() + "\n";
    Calibration cal = imp.getCalibration();
    int stackSize = imp.getStackSize();
    int channels = imp.getNChannels();
    int slices = imp.getNSlices();
    int frames = imp.getNFrames();
    int digits = imp.getBitDepth() == 32 ? 4 : 0;
    if (cal.scaled()) {
      String unit = cal.getUnit();
      String units = cal.getUnits();
      s +=
          "Width:  "
              + IJ.d2s(imp.getWidth() * cal.pixelWidth, 2)
              + " "
              + units
              + " ("
              + imp.getWidth()
              + ")\n";
      s +=
          "Height:  "
              + IJ.d2s(imp.getHeight() * cal.pixelHeight, 2)
              + " "
              + units
              + " ("
              + imp.getHeight()
              + ")\n";
      if (slices > 1)
        s += "Depth:  " + IJ.d2s(slices * cal.pixelDepth, 2) + " " + units + " (" + slices + ")\n";
      double xResolution = 1.0 / cal.pixelWidth;
      double yResolution = 1.0 / cal.pixelHeight;
      int places = Tools.getDecimalPlaces(xResolution, yResolution);
      if (xResolution == yResolution)
        s += "Resolution:  " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n";
      else {
        s += "X Resolution:  " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n";
        s += "Y Resolution:  " + IJ.d2s(yResolution, places) + " pixels per " + unit + "\n";
      }
    } else {
      s += "Width:  " + imp.getWidth() + " pixels\n";
      s += "Height:  " + imp.getHeight() + " pixels\n";
      if (stackSize > 1) s += "Depth:  " + slices + " pixels\n";
    }
    if (stackSize > 1)
      s +=
          "Voxel size: "
              + d2s(cal.pixelWidth)
              + "x"
              + d2s(cal.pixelHeight)
              + "x"
              + d2s(cal.pixelDepth)
              + " "
              + cal.getUnit()
              + "\n";
    else
      s +=
          "Pixel size: "
              + d2s(cal.pixelWidth)
              + "x"
              + d2s(cal.pixelHeight)
              + " "
              + cal.getUnit()
              + "\n";

    s += "ID: " + imp.getID() + "\n";
    String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : "";
    s += "Coordinate origin:  " + d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin + "\n";
    int type = imp.getType();
    switch (type) {
      case ImagePlus.GRAY8:
        s += "Bits per pixel: 8 ";
        String lut = "LUT";
        if (imp.getProcessor().isColorLut()) lut = "color " + lut;
        else lut = "grayscale " + lut;
        if (imp.isInvertedLut()) lut = "inverting " + lut;
        s += "(" + lut + ")\n";
        if (imp.getNChannels() > 1) s += displayRanges(imp);
        else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n";
        break;
      case ImagePlus.GRAY16:
      case ImagePlus.GRAY32:
        if (type == ImagePlus.GRAY16) {
          String sign = cal.isSigned16Bit() ? "signed" : "unsigned";
          s += "Bits per pixel: 16 (" + sign + ")\n";
        } else s += "Bits per pixel: 32 (float)\n";
        if (imp.getNChannels() > 1) s += displayRanges(imp);
        else {
          s += "Display range: ";
          double min = ip.getMin();
          double max = ip.getMax();
          if (cal.calibrated()) {
            min = cal.getCValue((int) min);
            max = cal.getCValue((int) max);
          }
          s += IJ.d2s(min, digits) + " - " + IJ.d2s(max, digits) + "\n";
        }
        break;
      case ImagePlus.COLOR_256:
        s += "Bits per pixel: 8 (color LUT)\n";
        break;
      case ImagePlus.COLOR_RGB:
        s += "Bits per pixel: 32 (RGB)\n";
        break;
    }
    double interval = cal.frameInterval;
    double fps = cal.fps;
    if (stackSize > 1) {
      ImageStack stack = imp.getStack();
      int slice = imp.getCurrentSlice();
      String number = slice + "/" + stackSize;
      String label = stack.getShortSliceLabel(slice);
      if (label != null && label.length() > 0) label = " (" + label + ")";
      else label = "";
      if (interval > 0.0 || fps != 0.0) {
        s += "Frame: " + number + label + "\n";
        if (fps != 0.0) {
          String sRate =
              Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5);
          s += "Frame rate: " + sRate + " fps\n";
        }
        if (interval != 0.0)
          s +=
              "Frame interval: "
                  + ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5))
                  + " "
                  + cal.getTimeUnit()
                  + "\n";
      } else s += "Image: " + number + label + "\n";
      if (imp.isHyperStack()) {
        if (channels > 1) s += "  Channel: " + imp.getChannel() + "/" + channels + "\n";
        if (slices > 1) s += "  Slice: " + imp.getSlice() + "/" + slices + "\n";
        if (frames > 1) s += "  Frame: " + imp.getFrame() + "/" + frames + "\n";
      }
      if (imp.isComposite()) {
        if (!imp.isHyperStack() && channels > 1) s += "  Channels: " + channels + "\n";
        String mode = ((CompositeImage) imp).getModeAsString();
        s += "  Composite mode: \"" + mode + "\"\n";
      }
    }

    if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No Threshold\n";
    else {
      double lower = ip.getMinThreshold();
      double upper = ip.getMaxThreshold();
      int dp = digits;
      if (cal.calibrated()) {
        lower = cal.getCValue((int) lower);
        upper = cal.getCValue((int) upper);
        dp = cal.isSigned16Bit() ? 0 : 4;
      }
      s += "Threshold: " + IJ.d2s(lower, dp) + "-" + IJ.d2s(upper, dp) + "\n";
    }
    ImageCanvas ic = imp.getCanvas();
    double mag = ic != null ? ic.getMagnification() : 1.0;
    if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n";

    if (cal.calibrated()) {
      s += " \n";
      int curveFit = cal.getFunction();
      s += "Calibration Function: ";
      if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n";
      else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n";
      else s += CurveFitter.fList[curveFit] + "\n";
      double[] c = cal.getCoefficients();
      if (c != null) {
        s += "  a: " + IJ.d2s(c[0], 6) + "\n";
        s += "  b: " + IJ.d2s(c[1], 6) + "\n";
        if (c.length >= 3) s += "  c: " + IJ.d2s(c[2], 6) + "\n";
        if (c.length >= 4) s += "  c: " + IJ.d2s(c[3], 6) + "\n";
        if (c.length >= 5) s += "  c: " + IJ.d2s(c[4], 6) + "\n";
      }
      s += "  Unit: \"" + cal.getValueUnit() + "\"\n";
    } else s += "Uncalibrated\n";

    FileInfo fi = imp.getOriginalFileInfo();
    if (fi != null) {
      if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n";
      else if (fi.directory != null && fi.fileName != null)
        s += "Path: " + fi.directory + fi.fileName + "\n";
    }

    ImageWindow win = imp.getWindow();
    if (win != null) {
      Point loc = win.getLocation();
      Dimension screen = IJ.getScreenSize();
      s +=
          "Screen location: "
              + loc.x
              + ","
              + loc.y
              + " ("
              + screen.width
              + "x"
              + screen.height
              + ")\n";
    }

    Overlay overlay = imp.getOverlay();
    if (overlay != null) {
      String hidden = imp.getHideOverlay() ? " (hidden)" : " ";
      int n = overlay.size();
      String elements = n == 1 ? " element" : " elements";
      s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n";
    } else s += "No Overlay\n";

    Roi roi = imp.getRoi();
    if (roi == null) {
      if (cal.calibrated()) s += " \n";
      s += "No Selection\n";
    } else if (roi instanceof EllipseRoi) {
      s += "\nElliptical Selection\n";
      double[] p = ((EllipseRoi) roi).getParams();
      double dx = p[2] - p[0];
      double dy = p[3] - p[1];
      double major = Math.sqrt(dx * dx + dy * dy);
      s += "  Major: " + IJ.d2s(major, 2) + "\n";
      s += "  Minor: " + IJ.d2s(major * p[4], 2) + "\n";
      s += "  X1: " + IJ.d2s(p[0], 2) + "\n";
      s += "  Y1: " + IJ.d2s(p[1], 2) + "\n";
      s += "  X2: " + IJ.d2s(p[2], 2) + "\n";
      s += "  Y2: " + IJ.d2s(p[3], 2) + "\n";
      s += "  Aspect ratio: " + IJ.d2s(p[4], 2) + "\n";
    } else {
      s += " \n";
      s += roi.getTypeAsString() + " Selection";
      String points = null;
      if (roi instanceof PointRoi) {
        int npoints = ((PolygonRoi) roi).getNCoordinates();
        String suffix = npoints > 1 ? "s)" : ")";
        points = " (" + npoints + " point" + suffix;
      }
      String name = roi.getName();
      if (name != null) {
        s += " (\"" + name + "\")";
        if (points != null) s += "\n " + points;
      } else if (points != null) s += points;
      s += "\n";
      Rectangle r = roi.getBounds();
      if (roi instanceof Line) {
        Line line = (Line) roi;
        s += "  X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n";
        s += "  Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n";
        s += "  X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n";
        s += "  Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n";
      } else if (cal.scaled()) {
        s += "  X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n";
        s += "  Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n";
        s += "  Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n";
        s += "  Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n";
      } else {
        s += "  X: " + r.x + "\n";
        s += "  Y: " + yy(r.y, imp) + "\n";
        s += "  Width: " + r.width + "\n";
        s += "  Height: " + r.height + "\n";
      }
    }

    return s;
  }
Пример #15
0
  void setCalibration(ImagePlus imp) {
    if (fi.fileType == FileInfo.GRAY16_SIGNED) {
      if (IJ.debugMode) IJ.log("16-bit signed");
      double[] coeff = new double[2];
      coeff[0] = -32768.0;
      coeff[1] = 1.0;
      imp.getLocalCalibration().setFunction(Calibration.STRAIGHT_LINE, coeff, "gray value");
    }

    Properties props = decodeDescriptionString(fi);
    Calibration cal = imp.getCalibration();
    boolean calibrated = false;
    if (fi.pixelWidth > 0.0 && fi.unit != null) {
      cal.pixelWidth = fi.pixelWidth;
      cal.pixelHeight = fi.pixelHeight;
      cal.pixelDepth = fi.pixelDepth;
      cal.setUnit(fi.unit);
      calibrated = true;
    }

    if (fi.valueUnit != null) {
      int f = fi.calibrationFunction;
      if ((f >= Calibration.STRAIGHT_LINE && f <= Calibration.RODBARD2 && fi.coefficients != null)
          || f == Calibration.UNCALIBRATED_OD) {
        boolean zeroClip = props != null && props.getProperty("zeroclip", "false").equals("true");
        cal.setFunction(f, fi.coefficients, fi.valueUnit, zeroClip);
        calibrated = true;
      }
    }

    if (calibrated) checkForCalibrationConflict(imp, cal);

    if (fi.frameInterval != 0.0) cal.frameInterval = fi.frameInterval;

    if (props == null) return;

    cal.xOrigin = getDouble(props, "xorigin");
    cal.yOrigin = getDouble(props, "yorigin");
    cal.zOrigin = getDouble(props, "zorigin");
    cal.info = props.getProperty("info");

    cal.fps = getDouble(props, "fps");
    cal.loop = getBoolean(props, "loop");
    cal.frameInterval = getDouble(props, "finterval");
    cal.setTimeUnit(props.getProperty("tunit", "sec"));

    double displayMin = getDouble(props, "min");
    double displayMax = getDouble(props, "max");
    if (!(displayMin == 0.0 && displayMax == 0.0)) {
      int type = imp.getType();
      ImageProcessor ip = imp.getProcessor();
      if (type == ImagePlus.GRAY8 || type == ImagePlus.COLOR_256)
        ip.setMinAndMax(displayMin, displayMax);
      else if (type == ImagePlus.GRAY16 || type == ImagePlus.GRAY32) {
        if (ip.getMin() != displayMin || ip.getMax() != displayMax)
          ip.setMinAndMax(displayMin, displayMax);
      }
    }

    int stackSize = imp.getStackSize();
    if (stackSize > 1) {
      int channels = (int) getDouble(props, "channels");
      int slices = (int) getDouble(props, "slices");
      int frames = (int) getDouble(props, "frames");
      if (channels == 0) channels = 1;
      if (slices == 0) slices = 1;
      if (frames == 0) frames = 1;
      // IJ.log("setCalibration: "+channels+"  "+slices+"  "+frames);
      if (channels * slices * frames == stackSize) {
        imp.setDimensions(channels, slices, frames);
        if (getBoolean(props, "hyperstack")) imp.setOpenAsHyperStack(true);
      }
    }
  }
Пример #16
0
    ImageProcessor setup(ImagePlus imp) {

      ImageProcessor ip;
      int type = imp.getType();
      if (type != ImagePlus.COLOR_RGB) return null;
      ip = imp.getProcessor();
      int id = imp.getID();
      int slice = imp.getCurrentSlice();

      if ((id != previousImageID) | (slice != previousSlice) | (flag)) {
        flag = false; // if true, flags a change from HSB to RGB or viceversa
        numSlices = imp.getStackSize();
        stack = imp.getStack();
        width = stack.getWidth();
        height = stack.getHeight();
        numPixels = width * height;

        hSource = new byte[numPixels];
        sSource = new byte[numPixels];
        bSource = new byte[numPixels];

        // restore = (int[])ip.getPixelsCopy(); //This runs into trouble sometimes, so do it the
        // long way:
        int[] temp = (int[]) ip.getPixels();
        restore = new int[numPixels];
        for (int i = 0; i < numPixels; i++) restore[i] = temp[i];

        fillMask = new int[numPixels];

        // Get hsb or rgb from image.
        ColorProcessor cp = (ColorProcessor) ip;
        IJ.showStatus("Gathering data");

        if (isRGB) cp.getRGB(hSource, sSource, bSource);
        else cp.getHSB(hSource, sSource, bSource);

        IJ.showStatus("done");

        // Create a spectrum ColorModel for the Hue histogram plot.
        Color c;
        byte[] reds = new byte[256];
        byte[] greens = new byte[256];
        byte[] blues = new byte[256];
        for (int i = 0; i < 256; i++) {
          c = Color.getHSBColor(i / 255f, 1f, 1f);

          reds[i] = (byte) c.getRed();
          greens[i] = (byte) c.getGreen();
          blues[i] = (byte) c.getBlue();
        }
        ColorModel cm = new IndexColorModel(8, 256, reds, greens, blues);

        // Make an image with just the hue from the RGB image and the spectrum LUT.
        // This is just for a hue histogram for the plot.  Do not show it.
        // ByteProcessor bpHue = new ByteProcessor(width,height,h,cm);
        ByteProcessor bpHue = new ByteProcessor(width, height, hSource, cm);
        ImagePlus impHue = new ImagePlus("Hue", bpHue);
        // impHue.show();

        ByteProcessor bpSat = new ByteProcessor(width, height, sSource, cm);
        ImagePlus impSat = new ImagePlus("Sat", bpSat);
        // impSat.show();

        ByteProcessor bpBri = new ByteProcessor(width, height, bSource, cm);
        ImagePlus impBri = new ImagePlus("Bri", bpBri);
        // impBri.show();

        plot.setHistogram(impHue, 0);
        splot.setHistogram(impSat, 1);
        bplot.setHistogram(impBri, 2);

        updateLabels();
        updatePlot();
        updateScrollBars();
        imp.updateAndDraw();
      }
      previousImageID = id;
      previousSlice = slice;
      return ip;
    }
Пример #17
0
  boolean showDialog(ImageProcessor ip) {
    String macroOptions = Macro.getOptions();
    if (macroOptions != null) {
      if (macroOptions.indexOf(" interpolate") != -1)
        macroOptions.replaceAll(" interpolate", " interpolation=Bilinear");
      else if (macroOptions.indexOf(" interpolation=") == -1)
        macroOptions = macroOptions + " interpolation=None";
      Macro.setOptions(macroOptions);
    }
    int bitDepth = imp.getBitDepth();
    int stackSize = imp.getStackSize();
    boolean isStack = stackSize > 1;
    oldDepth = stackSize;
    if (isStack) {
      xstr = "1.0";
      ystr = "1.0";
      zstr = "1.0";
    }
    r = ip.getRoi();
    int width = newWidth;
    if (width == 0) width = r.width;
    int height = (int) ((double) width * r.height / r.width);
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    zscale = 1.0;
    if (xscale != 0.0 && yscale != 0.0) {
      width = (int) (r.width * xscale);
      height = (int) (r.height * yscale);
    } else {
      xstr = "-";
      ystr = "-";
    }
    GenericDialog gd = new GenericDialog("Scale");
    gd.addStringField("X Scale:", xstr);
    gd.addStringField("Y Scale:", ystr);
    if (isStack) gd.addStringField("Z Scale:", zstr);
    gd.setInsets(5, 0, 5);
    gd.addStringField("Width (pixels):", "" + width);
    gd.addStringField("Height (pixels):", "" + height);
    if (isStack) {
      String label = "Depth (images):";
      if (imp.isHyperStack()) {
        int slices = imp.getNSlices();
        int frames = imp.getNFrames();
        if (slices == 1 && frames > 1) {
          label = "Depth (frames):";
          oldDepth = frames;
        } else {
          label = "Depth (slices):";
          oldDepth = slices;
        }
      }
      gd.addStringField(label, "" + oldDepth);
    }
    fields = gd.getStringFields();
    for (int i = 0; i < fields.size(); i++) {
      ((TextField) fields.elementAt(i)).addTextListener(this);
      ((TextField) fields.elementAt(i)).addFocusListener(this);
    }
    xField = (TextField) fields.elementAt(0);
    yField = (TextField) fields.elementAt(1);
    if (isStack) {
      zField = (TextField) fields.elementAt(2);
      widthField = (TextField) fields.elementAt(3);
      heightField = (TextField) fields.elementAt(4);
      depthField = (TextField) fields.elementAt(5);
    } else {
      widthField = (TextField) fields.elementAt(2);
      heightField = (TextField) fields.elementAt(3);
    }
    fieldWithFocus = xField;
    gd.addChoice("Interpolation:", methods, methods[interpolationMethod]);
    if (bitDepth == 8 || bitDepth == 24)
      gd.addCheckbox("Fill with background color", fillWithBackground);
    gd.addCheckbox("Average when downsizing", averageWhenDownsizing);
    boolean hyperstack = imp.isHyperStack() || imp.isComposite();
    if (isStack && !hyperstack) gd.addCheckbox("Process entire stack", processStack);
    gd.addCheckbox("Create new window", newWindow);
    title = WindowManager.getUniqueName(imp.getTitle());
    gd.setInsets(10, 0, 0);
    gd.addStringField("Title:", title, 12);
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    xstr = gd.getNextString();
    ystr = gd.getNextString();
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    if (isStack) {
      zstr = gd.getNextString();
      zscale = Tools.parseDouble(ystr, 0.0);
    }
    String wstr = gd.getNextString();
    newWidth = (int) Tools.parseDouble(wstr, 0);
    newHeight = (int) Tools.parseDouble(gd.getNextString(), 0);
    if (newHeight != 0 && (wstr.equals("-") || wstr.equals("0")))
      newWidth = (int) (newHeight * (double) r.width / r.height);
    if (newWidth == 0 || newHeight == 0) {
      IJ.error("Scaler", "Width or height is 0");
      return false;
    }
    if (xscale > 0.0 && yscale > 0.0) {
      newWidth = (int) (r.width * xscale);
      newHeight = (int) (r.height * yscale);
    }
    if (isStack) newDepth = (int) Tools.parseDouble(gd.getNextString(), 0);
    interpolationMethod = gd.getNextChoiceIndex();
    if (bitDepth == 8 || bitDepth == 24) fillWithBackground = gd.getNextBoolean();
    averageWhenDownsizing = gd.getNextBoolean();
    if (isStack && !hyperstack) processStack = gd.getNextBoolean();
    if (hyperstack) processStack = true;
    newWindow = gd.getNextBoolean();
    if (xscale == 0.0) {
      xscale = (double) newWidth / r.width;
      yscale = (double) newHeight / r.height;
    }
    title = gd.getNextString();

    if (fillWithBackground) {
      Color bgc = Toolbar.getBackgroundColor();
      if (bitDepth == 8) bgValue = ip.getBestIndex(bgc);
      else if (bitDepth == 24) bgValue = bgc.getRGB();
    } else bgValue = 0.0;
    return true;
  }
Пример #18
0
 public void textValueChanged(TextEvent e) {
   Object source = e.getSource();
   double newXScale = xscale;
   double newYScale = yscale;
   double newZScale = zscale;
   if (source == xField && fieldWithFocus == xField) {
     String newXText = xField.getText();
     newXScale = Tools.parseDouble(newXText, 0);
     if (newXScale == 0) return;
     if (newXScale != xscale) {
       int newWidth = (int) (newXScale * r.width);
       widthField.setText("" + newWidth);
       if (constainAspectRatio) {
         yField.setText(newXText);
         int newHeight = (int) (newXScale * r.height);
         heightField.setText("" + newHeight);
       }
     }
   } else if (source == yField && fieldWithFocus == yField) {
     String newYText = yField.getText();
     newYScale = Tools.parseDouble(newYText, 0);
     if (newYScale == 0) return;
     if (newYScale != yscale) {
       int newHeight = (int) (newYScale * r.height);
       heightField.setText("" + newHeight);
     }
   } else if (source == zField && fieldWithFocus == zField) {
     String newZText = zField.getText();
     newZScale = Tools.parseDouble(newZText, 0);
     if (newZScale == 0) return;
     if (newZScale != zscale) {
       int nSlices = imp.getStackSize();
       if (imp.isHyperStack()) {
         int slices = imp.getNSlices();
         int frames = imp.getNFrames();
         if (slices == 1 && frames > 1) nSlices = frames;
         else nSlices = slices;
       }
       int newDepth = (int) (newZScale * nSlices);
       depthField.setText("" + newDepth);
     }
   } else if (source == widthField && fieldWithFocus == widthField) {
     int newWidth = (int) Tools.parseDouble(widthField.getText(), 0.0);
     if (newWidth != 0) {
       int newHeight = (int) (newWidth * (double) r.height / r.width);
       heightField.setText("" + newHeight);
       xField.setText("-");
       yField.setText("-");
       newXScale = 0.0;
       newYScale = 0.0;
     }
   } else if (source == depthField && fieldWithFocus == depthField) {
     int newDepth = (int) Tools.parseDouble(depthField.getText(), 0.0);
     if (newDepth != 0) {
       zField.setText("-");
       newZScale = 0.0;
     }
   }
   xscale = newXScale;
   yscale = newYScale;
   zscale = newZScale;
 }
Пример #19
0
  boolean showDialog(ImageProcessor ip) {
    int bitDepth = imp.getBitDepth();
    boolean isStack = imp.getStackSize() > 1;
    r = ip.getRoi();
    int width = newWidth;
    if (width == 0) width = r.width;
    int height = (int) ((double) width * r.height / r.width);
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    if (xscale != 0.0 && yscale != 0.0) {
      width = (int) (r.width * xscale);
      height = (int) (r.height * yscale);
    } else {
      xstr = "-";
      ystr = "-";
    }
    GenericDialog gd = new GenericDialog("Scale");
    gd.addStringField("X Scale (0.05-25):", xstr);
    gd.addStringField("Y Scale (0.05-25):", ystr);
    gd.setInsets(5, 0, 5);
    gd.addStringField("Width (pixels):", "" + width);
    gd.addStringField("Height (pixels):", "" + height);
    fields = gd.getStringFields();
    for (int i = 0; i < 3; i++) {
      ((TextField) fields.elementAt(i)).addTextListener(this);
      ((TextField) fields.elementAt(i)).addFocusListener(this);
    }
    xField = (TextField) fields.elementAt(0);
    yField = (TextField) fields.elementAt(1);
    widthField = (TextField) fields.elementAt(2);
    heightField = (TextField) fields.elementAt(3);
    fieldWithFocus = xField;
    gd.addCheckbox("Interpolate", interpolate);
    if (bitDepth == 8 || bitDepth == 24)
      gd.addCheckbox("Fill with Background Color", fillWithBackground);
    if (isStack) gd.addCheckbox("Process Entire Stack", processStack);
    gd.addCheckbox("Create New Window", newWindow);
    title = WindowManager.getUniqueName(imp.getTitle());
    gd.setInsets(10, 0, 0);
    gd.addStringField("Title:", title, 12);
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    xstr = gd.getNextString();
    ystr = gd.getNextString();
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    String wstr = gd.getNextString();
    newWidth = (int) Tools.parseDouble(wstr, 0);
    newHeight = (int) Tools.parseDouble(gd.getNextString(), 0);
    if (newHeight != 0 && (wstr.equals("-") || wstr.equals("0")))
      newWidth = (int) (newHeight * (double) r.width / r.height);
    if (newWidth == 0 || newHeight == 0) {
      IJ.error("Invalid width or height entered");
      return false;
    }
    if (xscale > 25.0) xscale = 25.0;
    if (yscale > 25.0) yscale = 25.0;
    if (xscale > 0.0 && yscale > 0.0) {
      newWidth = (int) (r.width * xscale);
      newHeight = (int) (r.height * yscale);
    }
    interpolate = gd.getNextBoolean();
    if (bitDepth == 8 || bitDepth == 24) fillWithBackground = gd.getNextBoolean();
    if (isStack) processStack = gd.getNextBoolean();
    newWindow = gd.getNextBoolean();
    if (!newWindow && xscale == 0.0) {
      xscale = (double) newWidth / r.width;
      yscale = (double) newHeight / r.height;
    }
    title = gd.getNextString();

    if (fillWithBackground) {
      Color bgc = Toolbar.getBackgroundColor();
      if (bitDepth == 8) bgValue = ip.getBestIndex(bgc);
      else if (bitDepth == 24) bgValue = bgc.getRGB();
    } else {
      if (bitDepth == 8) bgValue = ip.isInvertedLut() ? 0.0 : 255.0; // white
      else if (bitDepth == 24) bgValue = 0xffffffff; // white
    }
    return true;
  }
  /**
   * Performs particle analysis on the specified ImagePlus and ImageProcessor. Returns false if
   * there is an error.
   */
  public boolean analyze(ImagePlus imp, ImageProcessor ip) {
    if (this.imp == null) this.imp = imp;
    showResults = (options & SHOW_RESULTS) != 0;
    excludeEdgeParticles = (options & EXCLUDE_EDGE_PARTICLES) != 0;
    resetCounter = (options & CLEAR_WORKSHEET) != 0;
    showProgress = (options & SHOW_PROGRESS) != 0;
    floodFill = (options & INCLUDE_HOLES) == 0;
    recordStarts = (options & RECORD_STARTS) != 0;
    addToManager = (options & ADD_TO_MANAGER) != 0;
    displaySummary = (options & DISPLAY_SUMMARY) != 0;
    inSituShow = (options & IN_SITU_SHOW) != 0;
    outputImage = null;
    ip.snapshot();
    ip.setProgressBar(null);
    if (Analyzer.isRedirectImage()) {
      redirectImp = Analyzer.getRedirectImage(imp);
      if (redirectImp == null) return false;
      int depth = redirectImp.getStackSize();
      if (depth > 1 && depth == imp.getStackSize()) {
        ImageStack redirectStack = redirectImp.getStack();
        redirectIP = redirectStack.getProcessor(imp.getCurrentSlice());
      } else redirectIP = redirectImp.getProcessor();
    } else if (imp.getType() == ImagePlus.COLOR_RGB) {
      ImagePlus original = (ImagePlus) imp.getProperty("OriginalImage");
      if (original != null
          && original.getWidth() == imp.getWidth()
          && original.getHeight() == imp.getHeight()) {
        redirectImp = original;
        redirectIP = original.getProcessor();
      }
    }
    if (!setThresholdLevels(imp, ip)) return false;
    width = ip.getWidth();
    height = ip.getHeight();
    if (!(showChoice == NOTHING || showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS)) {
      blackBackground = Prefs.blackBackground && inSituShow;
      if (slice == 1) outlines = new ImageStack(width, height);
      if (showChoice == ROI_MASKS) drawIP = new ShortProcessor(width, height);
      else drawIP = new ByteProcessor(width, height);
      drawIP.setLineWidth(lineWidth);
      if (showChoice == ROI_MASKS) {
      } // Place holder for now...
      else if (showChoice == MASKS && !blackBackground) drawIP.invertLut();
      else if (showChoice == OUTLINES) {
        if (!inSituShow) {
          if (customLut == null) makeCustomLut();
          drawIP.setColorModel(customLut);
        }
        drawIP.setFont(new Font("SansSerif", Font.PLAIN, fontSize));
        if (fontSize > 12 && inSituShow) drawIP.setAntialiasedText(true);
      }
      outlines.addSlice(null, drawIP);

      if (showChoice == ROI_MASKS || blackBackground) {
        drawIP.setColor(Color.black);
        drawIP.fill();
        drawIP.setColor(Color.white);
      } else {
        drawIP.setColor(Color.white);
        drawIP.fill();
        drawIP.setColor(Color.black);
      }
    }
    calibration = redirectImp != null ? redirectImp.getCalibration() : imp.getCalibration();

    if (rt == null) {
      rt = Analyzer.getResultsTable();
      analyzer = new Analyzer(imp);
    } else analyzer = new Analyzer(imp, measurements, rt);
    if (resetCounter && slice == 1) {
      if (!Analyzer.resetCounter()) return false;
    }
    beginningCount = Analyzer.getCounter();

    byte[] pixels = null;
    if (ip instanceof ByteProcessor) pixels = (byte[]) ip.getPixels();
    if (r == null) {
      r = ip.getRoi();
      mask = ip.getMask();
      if (displaySummary) {
        if (mask != null) totalArea = ImageStatistics.getStatistics(ip, AREA, calibration).area;
        else totalArea = r.width * calibration.pixelWidth * r.height * calibration.pixelHeight;
      }
    }
    minX = r.x;
    maxX = r.x + r.width;
    minY = r.y;
    maxY = r.y + r.height;
    if (r.width < width || r.height < height || mask != null) {
      if (!eraseOutsideRoi(ip, r, mask)) return false;
    }
    int offset;
    double value;
    int inc = Math.max(r.height / 25, 1);
    int mi = 0;
    ImageWindow win = imp.getWindow();
    if (win != null) win.running = true;
    if (measurements == 0) measurements = Analyzer.getMeasurements();
    if (showChoice == ELLIPSES) measurements |= ELLIPSE;
    measurements &= ~LIMIT; // ignore "Limit to Threshold"
    roiNeedsImage =
        (measurements & PERIMETER) != 0
            || (measurements & SHAPE_DESCRIPTORS) != 0
            || (measurements & FERET) != 0;
    particleCount = 0;
    wand = new Wand(ip);
    pf = new PolygonFiller();
    if (floodFill) {
      ImageProcessor ipf = ip.duplicate();
      ipf.setValue(fillColor);
      ff = new FloodFiller(ipf);
    }
    roiType = Wand.allPoints() ? Roi.FREEROI : Roi.TRACED_ROI;

    for (int y = r.y; y < (r.y + r.height); y++) {
      offset = y * width;
      for (int x = r.x; x < (r.x + r.width); x++) {
        if (pixels != null) value = pixels[offset + x] & 255;
        else if (imageType == SHORT) value = ip.getPixel(x, y);
        else value = ip.getPixelValue(x, y);
        if (value >= level1 && value <= level2) analyzeParticle(x, y, imp, ip);
      }
      if (showProgress && ((y % inc) == 0)) IJ.showProgress((double) (y - r.y) / r.height);
      if (win != null) canceled = !win.running;
      if (canceled) {
        Macro.abort();
        break;
      }
    }
    if (showProgress) IJ.showProgress(1.0);
    if (showResults) rt.updateResults();
    imp.killRoi();
    ip.resetRoi();
    ip.reset();
    if (displaySummary && IJ.getInstance() != null) updateSliceSummary();
    if (addToManager && roiManager != null) roiManager.setEditMode(imp, true);
    maxParticleCount = (particleCount > maxParticleCount) ? particleCount : maxParticleCount;
    totalCount += particleCount;
    if (!canceled) showResults();
    return true;
  }