Example #1
0
 boolean validDialogValues() {
   Calibration cal = imp.getCalibration();
   double pw = cal.pixelWidth, ph = cal.pixelHeight;
   if (width / pw < 1 || height / ph < 1) return false;
   if (xRoi / pw > imp.getWidth() || yRoi / ph > imp.getHeight()) return false;
   return true;
 }
Example #2
0
  /*------------------------------------------------------------------*/
  void setupProgressBar() {
    int height = imp.getHeight();
    int width = imp.getWidth();

    completed = 0;
    lastTime = System.currentTimeMillis();
    switch (operation) {
      case GRADIENT_MAGNITUDE:
        processDuration = stackSize * (width + 2 * height);
        break;
      case GRADIENT_DIRECTION:
        processDuration = stackSize * (width + 2 * height);
        break;
      case LAPLACIAN:
        processDuration = stackSize * (width + 2 * height);
        break;
      case LARGEST_HESSIAN:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      case SMALLEST_HESSIAN:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      case HESSIAN_ORIENTATION:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      default:
        throw new IllegalArgumentException("Invalid operation");
    }
  } /* end setupProgressBar */
Example #3
0
  /** Generate output image whose type is same as input image. */
  private ImagePlus makeOutputImage(ImagePlus imp, FloatProcessor fp, int ptype) {
    int width = imp.getWidth();
    int height = imp.getHeight();
    float[] pixels = (float[]) fp.getPixels();
    ImageProcessor oip = null;

    // Create output image consistent w/ type of input image.
    int size = pixels.length;
    switch (ptype) {
      case BYTE_TYPE:
        oip = imp.getProcessor().createProcessor(width, height);
        byte[] pixels8 = (byte[]) oip.getPixels();
        for (int i = 0; i < size; i++) pixels8[i] = (byte) pixels[i];
        break;
      case SHORT_TYPE:
        oip = imp.getProcessor().createProcessor(width, height);
        short[] pixels16 = (short[]) oip.getPixels();
        for (int i = 0; i < size; i++) pixels16[i] = (short) pixels[i];
        break;
      case FLOAT_TYPE:
        oip = new FloatProcessor(width, height, pixels, null);
        break;
    }

    // Adjust for display.
    // Calling this on non-ByteProcessors ensures image
    // processor is set up to correctly display image.
    oip.resetMinAndMax();

    // Create new image plus object. Don't use
    // ImagePlus.createImagePlus here because there may be
    // attributes of input image that are not appropriate for
    // projection.
    return new ImagePlus(makeTitle(), oip);
  }
Example #4
0
 void createNewStack(ImagePlus imp, ImageProcessor ip) {
   int nSlices = imp.getStackSize();
   int w = imp.getWidth(), h = imp.getHeight();
   ImagePlus imp2 = imp.createImagePlus();
   Rectangle r = ip.getRoi();
   boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight();
   ImageStack stack1 = imp.getStack();
   ImageStack stack2 = new ImageStack(newWidth, newHeight);
   ImageProcessor ip1, ip2;
   int method = interpolationMethod;
   if (w == 1 || h == 1) method = ImageProcessor.NONE;
   for (int i = 1; i <= nSlices; i++) {
     IJ.showStatus("Scale: " + i + "/" + nSlices);
     ip1 = stack1.getProcessor(i);
     String label = stack1.getSliceLabel(i);
     if (crop) {
       ip1.setRoi(r);
       ip1 = ip1.crop();
     }
     ip1.setInterpolationMethod(method);
     ip2 = ip1.resize(newWidth, newHeight, averageWhenDownsizing);
     if (ip2 != null) stack2.addSlice(label, ip2);
     IJ.showProgress(i, nSlices);
   }
   imp2.setStack(title, stack2);
   Calibration cal = imp2.getCalibration();
   if (cal.scaled()) {
     cal.pixelWidth *= 1.0 / xscale;
     cal.pixelHeight *= 1.0 / yscale;
   }
   IJ.showProgress(1.0);
   int[] dim = imp.getDimensions();
   imp2.setDimensions(dim[2], dim[3], dim[4]);
   if (imp.isComposite()) {
     imp2 = new CompositeImage(imp2, ((CompositeImage) imp).getMode());
     ((CompositeImage) imp2).copyLuts(imp);
   }
   if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true);
   if (newDepth > 0 && newDepth != oldDepth)
     imp2 = (new Resizer()).zScale(imp2, newDepth, interpolationMethod);
   if (imp2 != null) {
     imp2.show();
     imp2.changes = true;
   }
 }
 void updateImage(ImagePlus imp) {
   this.imp = imp;
   int width = imp.getWidth();
   int height = imp.getHeight();
   imageWidth = width;
   imageHeight = height;
   srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
   setDrawingSize(imageWidth, (int) imageHeight);
   magnification = 1.0;
 }
Example #6
0
  /** Performs actual projection using specified method. */
  public void doProjection() {
    if (imp == null) return;
    sliceCount = 0;
    if (method < AVG_METHOD || method > MEDIAN_METHOD) method = AVG_METHOD;
    for (int slice = startSlice; slice <= stopSlice; slice += increment) sliceCount++;
    if (method == MEDIAN_METHOD) {
      projImage = doMedianProjection();
      return;
    }

    // Create new float processor for projected pixels.
    FloatProcessor fp = new FloatProcessor(imp.getWidth(), imp.getHeight());
    ImageStack stack = imp.getStack();
    RayFunction rayFunc = getRayFunction(method, fp);
    if (IJ.debugMode == true) {
      IJ.log("\nProjecting stack from: " + startSlice + " to: " + stopSlice);
    }

    // Determine type of input image. Explicit determination of
    // processor type is required for subsequent pixel
    // manipulation.  This approach is more efficient than the
    // more general use of ImageProcessor's getPixelValue and
    // putPixel methods.
    int ptype;
    if (stack.getProcessor(1) instanceof ByteProcessor) ptype = BYTE_TYPE;
    else if (stack.getProcessor(1) instanceof ShortProcessor) ptype = SHORT_TYPE;
    else if (stack.getProcessor(1) instanceof FloatProcessor) ptype = FLOAT_TYPE;
    else {
      IJ.error("Z Project", "Non-RGB stack required");
      return;
    }

    // Do the projection.
    for (int n = startSlice; n <= stopSlice; n += increment) {
      IJ.showStatus("ZProjection " + color + ": " + n + "/" + stopSlice);
      IJ.showProgress(n - startSlice, stopSlice - startSlice);
      projectSlice(stack.getPixels(n), rayFunc, ptype);
    }

    // Finish up projection.
    if (method == SUM_METHOD) {
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else if (method == SD_METHOD) {
      rayFunc.postProcess();
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else {
      rayFunc.postProcess();
      projImage = makeOutputImage(imp, fp, ptype);
    }

    if (projImage == null) IJ.error("Z Project", "Error computing projection.");
  }
Example #7
0
 void createNewStack(ImagePlus imp, ImageProcessor ip) {
   Rectangle r = ip.getRoi();
   boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight();
   int nSlices = imp.getStackSize();
   ImageStack stack1 = imp.getStack();
   ImageStack stack2 = new ImageStack(newWidth, newHeight);
   ImageProcessor ip1, ip2;
   boolean interp = interpolate;
   if (imp.getWidth() == 1 || imp.getHeight() == 1) interp = false;
   for (int i = 1; i <= nSlices; i++) {
     IJ.showStatus("Scale: " + i + "/" + nSlices);
     ip1 = stack1.getProcessor(i);
     String label = stack1.getSliceLabel(i);
     if (crop) {
       ip1.setRoi(r);
       ip1 = ip1.crop();
     }
     ip1.setInterpolate(interp);
     ip2 = ip1.resize(newWidth, newHeight);
     if (ip2 != null) stack2.addSlice(label, ip2);
     IJ.showProgress(i, nSlices);
   }
   ImagePlus imp2 = imp.createImagePlus();
   imp2.setStack(title, stack2);
   Calibration cal = imp2.getCalibration();
   if (cal.scaled()) {
     cal.pixelWidth *= 1.0 / xscale;
     cal.pixelHeight *= 1.0 / yscale;
   }
   int[] dim = imp.getDimensions();
   imp2.setDimensions(dim[2], dim[3], dim[4]);
   IJ.showProgress(1.0);
   if (imp.isComposite()) {
     imp2 = new CompositeImage(imp2, 0);
     ((CompositeImage) imp2).copyLuts(imp);
   }
   if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true);
   imp2.show();
   imp2.changes = true;
 }
 protected void handleRoiMouseDown(MouseEvent e) {
   int sx = e.getX();
   int sy = e.getY();
   int ox = offScreenX(sx);
   int oy = offScreenY(sy);
   Roi roi = imp.getRoi();
   int handle = roi != null ? roi.isHandle(sx, sy) : -1;
   boolean multiPointMode =
       roi != null
           && (roi instanceof PointRoi)
           && handle == -1
           && Toolbar.getToolId() == Toolbar.POINT
           && Toolbar.getMultiPointMode();
   if (multiPointMode) {
     imp.setRoi(((PointRoi) roi).addPoint(ox, oy));
     return;
   }
   setRoiModState(e, roi, handle);
   if (roi != null) {
     if (handle >= 0) {
       roi.mouseDownInHandle(handle, sx, sy);
       return;
     }
     Rectangle r = roi.getBounds();
     int type = roi.getType();
     if (type == Roi.RECTANGLE
         && r.width == imp.getWidth()
         && r.height == imp.getHeight()
         && roi.getPasteMode() == Roi.NOT_PASTING
         && !(roi instanceof ImageRoi)) {
       imp.killRoi();
       return;
     }
     if (roi.contains(ox, oy)) {
       if (roi.modState == Roi.NO_MODS) roi.handleMouseDown(sx, sy);
       else {
         imp.killRoi();
         imp.createNewRoi(sx, sy);
       }
       return;
     }
     if ((type == Roi.POLYGON || type == Roi.POLYLINE || type == Roi.ANGLE)
         && roi.getState() == roi.CONSTRUCTING) return;
     int tool = Toolbar.getToolId();
     if ((tool == Toolbar.POLYGON || tool == Toolbar.POLYLINE || tool == Toolbar.ANGLE)
         && !(IJ.shiftKeyDown() || IJ.altKeyDown())) {
       imp.killRoi();
       return;
     }
   }
   imp.createNewRoi(sx, sy);
 }
 public ImageCanvas(ImagePlus imp) {
   this.imp = imp;
   ij = IJ.getInstance();
   int width = imp.getWidth();
   int height = imp.getHeight();
   imageWidth = width;
   imageHeight = height;
   srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
   setDrawingSize(imageWidth, (int) (imageHeight));
   magnification = 1.0;
   addMouseListener(this);
   addMouseMotionListener(this);
   addKeyListener(ij); // ImageJ handles keyboard shortcuts
   setFocusTraversalKeysEnabled(false);
 }
Example #10
0
 private void doRGBProjection(ImageStack stack) {
   ImageStack[] channels = ChannelSplitter.splitRGB(stack, true);
   ImagePlus red = new ImagePlus("Red", channels[0]);
   ImagePlus green = new ImagePlus("Green", channels[1]);
   ImagePlus blue = new ImagePlus("Blue", channels[2]);
   imp.unlock();
   ImagePlus saveImp = imp;
   imp = red;
   color = "(red)";
   doProjection();
   ImagePlus red2 = projImage;
   imp = green;
   color = "(green)";
   doProjection();
   ImagePlus green2 = projImage;
   imp = blue;
   color = "(blue)";
   doProjection();
   ImagePlus blue2 = projImage;
   int w = red2.getWidth(), h = red2.getHeight(), d = red2.getStackSize();
   if (method == SD_METHOD) {
     ImageProcessor r = red2.getProcessor();
     ImageProcessor g = green2.getProcessor();
     ImageProcessor b = blue2.getProcessor();
     double max = 0;
     double rmax = r.getStatistics().max;
     if (rmax > max) max = rmax;
     double gmax = g.getStatistics().max;
     if (gmax > max) max = gmax;
     double bmax = b.getStatistics().max;
     if (bmax > max) max = bmax;
     double scale = 255 / max;
     r.multiply(scale);
     g.multiply(scale);
     b.multiply(scale);
     red2.setProcessor(r.convertToByte(false));
     green2.setProcessor(g.convertToByte(false));
     blue2.setProcessor(b.convertToByte(false));
   }
   RGBStackMerge merge = new RGBStackMerge();
   ImageStack stack2 =
       merge.mergeStacks(w, h, d, red2.getStack(), green2.getStack(), blue2.getStack(), true);
   imp = saveImp;
   projImage = new ImagePlus(makeTitle(), stack2);
 }
Example #11
0
 public void doHyperStackProjection(boolean allTimeFrames) {
   int start = startSlice;
   int stop = stopSlice;
   int firstFrame = 1;
   int lastFrame = imp.getNFrames();
   if (!allTimeFrames) firstFrame = lastFrame = imp.getFrame();
   ImageStack stack = new ImageStack(imp.getWidth(), imp.getHeight());
   int channels = imp.getNChannels();
   int slices = imp.getNSlices();
   if (slices == 1) {
     slices = imp.getNFrames();
     firstFrame = lastFrame = 1;
   }
   int frames = lastFrame - firstFrame + 1;
   increment = channels;
   boolean rgb = imp.getBitDepth() == 24;
   for (int frame = firstFrame; frame <= lastFrame; frame++) {
     for (int channel = 1; channel <= channels; channel++) {
       startSlice = (frame - 1) * channels * slices + (start - 1) * channels + channel;
       stopSlice = (frame - 1) * channels * slices + (stop - 1) * channels + channel;
       if (rgb) doHSRGBProjection(imp);
       else doProjection();
       stack.addSlice(null, projImage.getProcessor());
     }
   }
   projImage = new ImagePlus(makeTitle(), stack);
   projImage.setDimensions(channels, 1, frames);
   if (channels > 1) {
     projImage = new CompositeImage(projImage, 0);
     ((CompositeImage) projImage).copyLuts(imp);
     if (method == SUM_METHOD || method == SD_METHOD)
       ((CompositeImage) projImage).resetDisplayRanges();
   }
   if (frames > 1) projImage.setOpenAsHyperStack(true);
   Overlay overlay = imp.getOverlay();
   if (overlay != null) {
     startSlice = start;
     stopSlice = stop;
     if (imp.getType() == ImagePlus.COLOR_RGB)
       projImage.setOverlay(projectRGBHyperStackRois(overlay));
     else projImage.setOverlay(projectHyperStackRois(overlay));
   }
   IJ.showProgress(1, 1);
 }
Example #12
0
 public void run(String arg) {
   imp = IJ.getImage();
   if (imp == null) return;
   stackSize = imp.getStackSize();
   Roi roi = imp.getRoi();
   Calibration cal = imp.getCalibration();
   if (roi != null && roi.getBounds().equals(prevRoi) && cal.pixelWidth == prevPixelWidth)
     roi = null;
   if (roi != null) {
     boolean rectOrOval =
         roi != null && (roi.getType() == Roi.RECTANGLE || roi.getType() == Roi.OVAL);
     oval = rectOrOval && (roi.getType() == Roi.OVAL); // Handle existing oval ROI
     Rectangle r = roi.getBounds();
     width = r.width;
     height = r.height;
     xRoi = r.x;
     yRoi = r.y;
     if (scaledUnits && cal.scaled()) {
       xRoi = xRoi * cal.pixelWidth;
       yRoi = yRoi * cal.pixelHeight;
       width = width * cal.pixelWidth;
       height = height * cal.pixelHeight;
     }
     if (centered) { // Make xRoi and yRoi consistent when centered mode is active
       xRoi += width / 2.0;
       yRoi += height / 2.0;
     }
   } else if (!validDialogValues()) {
     width = imp.getWidth() / 2;
     height = imp.getHeight() / 2;
     xRoi = width / 2;
     yRoi = height / 2;
   }
   iSlice = imp.getCurrentSlice();
   showDialog();
 }