Beispiel #1
0
 private void openDirectory(File f, String path) {
   if (path == null) return;
   if (!(path.endsWith(File.separator) || path.endsWith("/"))) path += File.separator;
   String[] names = f.list();
   names = (new FolderOpener()).trimFileList(names);
   if (names == null) return;
   String msg = "Open all " + names.length + " images in \"" + f.getName() + "\" as a stack?";
   GenericDialog gd = new GenericDialog("Open Folder");
   gd.setInsets(10, 5, 0);
   gd.addMessage(msg);
   gd.setInsets(15, 35, 0);
   gd.addCheckbox("Convert to RGB", convertToRGB);
   gd.setInsets(0, 35, 0);
   gd.addCheckbox("Use Virtual Stack", virtualStack);
   gd.enableYesNoCancel();
   gd.showDialog();
   if (gd.wasCanceled()) return;
   if (gd.wasOKed()) {
     convertToRGB = gd.getNextBoolean();
     virtualStack = gd.getNextBoolean();
     String options = " sort";
     if (convertToRGB) options += " convert_to_rgb";
     if (virtualStack) options += " use";
     IJ.run("Image Sequence...", "open=[" + path + "]" + options);
     DirectoryChooser.setDefaultDirectory(path);
   } else {
     for (int k = 0; k < names.length; k++) {
       IJ.redirectErrorMessages();
       if (!names[k].startsWith(".")) (new Opener()).open(path + names[k]);
     }
   }
   IJ.register(DragAndDrop.class);
 }
Beispiel #2
0
  public boolean beadCalibration3d() {
    imp = IJ.getImage();
    if (imp == null) {
      IJ.noImage();
      return false;
    } else if (imp.getStackSize() == 1) {
      IJ.error("Stack required");
      return false;
    } else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
      // In order to support 32bit images, pict[] must be changed to float[], and  getPixel(x, y);
      // requires a Float.intBitsToFloat() conversion
      IJ.error("8 or 16 bit greyscale image required");
      return false;
    }
    width = imp.getWidth();
    height = imp.getHeight();
    nslices = imp.getStackSize();
    imtitle = imp.getTitle();

    models[0] = "*None*";
    models[1] = "line";
    models[2] = "2nd degree polynomial";
    models[3] = "3rd degree polynomial";
    models[4] = "4th degree polynomial";

    GenericDialog gd = new GenericDialog("3D PALM calibration");
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
    gd.addNumericField(
        "Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
    gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
    gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
    gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
    gd.addCheckbox(
        "Show divergence of bead positions against model",
        prefs.get("QuickPALM.3Dcal_showDivergence", false));
    gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
    gd.addMessage("\n\nDon't forget to save the table in the end...");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.pthrsh", pthrsh * 100);
    cal_z = gd.getNextNumber();
    prefs.set("QuickPALM.z-step", cal_z);
    window = (int) gd.getNextNumber();
    prefs.set("QuickPALM.window", window);
    model = gd.getNextChoice();
    prefs.set("QuickPALM.model", model);
    part_divergence = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
    part_extrainfo = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
    return true;
  }
Beispiel #3
0
 void addSelection() {
   ImagePlus imp = IJ.getImage();
   String macroOptions = Macro.getOptions();
   if (macroOptions != null && IJ.macroRunning() && macroOptions.indexOf("remove") != -1) {
     imp.setOverlay(null);
     return;
   }
   Roi roi = imp.getRoi();
   if (roi == null && imp.getOverlay() != null) {
     GenericDialog gd = new GenericDialog("No Selection");
     gd.addMessage("\"Overlay>Add\" requires a selection.");
     gd.setInsets(15, 40, 0);
     gd.addCheckbox("Remove existing overlay", false);
     gd.showDialog();
     if (gd.wasCanceled()) return;
     if (gd.getNextBoolean()) imp.setOverlay(null);
     return;
   }
   if (roi == null) {
     IJ.error("This command requires a selection.");
     return;
   }
   roi = (Roi) roi.clone();
   if (roi.getStrokeColor() == null) roi.setStrokeColor(Toolbar.getForegroundColor());
   int width = Line.getWidth();
   Rectangle bounds = roi.getBounds();
   boolean tooWide = width > Math.max(bounds.width, bounds.height) / 3.0;
   if (roi.getStroke() == null && width > 1 && !tooWide) roi.setStrokeWidth(Line.getWidth());
   Overlay overlay = imp.getOverlay();
   if (overlay != null && overlay.size() > 0 && !roi.isDrawingTool()) {
     Roi roi2 = overlay.get(overlay.size() - 1);
     if (roi.getStroke() == null) roi.setStrokeWidth(roi2.getStrokeWidth());
     if (roi.getFillColor() == null) roi.setFillColor(roi2.getFillColor());
   }
   boolean points = roi instanceof PointRoi && ((PolygonRoi) roi).getNCoordinates() > 1;
   if (points) roi.setStrokeColor(Color.red);
   if (!IJ.altKeyDown() && !(roi instanceof Arrow)) {
     RoiProperties rp = new RoiProperties("Add to Overlay", roi);
     if (!rp.showDialog()) return;
   }
   String name = roi.getName();
   boolean newOverlay = name != null && name.equals("new-overlay");
   if (overlay == null || newOverlay) overlay = new Overlay();
   overlay.add(roi);
   imp.setOverlay(overlay);
   overlay2 = overlay;
   if (points || (roi instanceof ImageRoi) || (roi instanceof Arrow)) imp.killRoi();
   Undo.setup(Undo.OVERLAY_ADDITION, imp);
 }
Beispiel #4
0
 int showDialog(String[] versions) {
   GenericDialog gd = new GenericDialog("ImageJ Updater");
   gd.addChoice("Upgrade To:", versions, versions[0]);
   String msg =
       "You are currently running v"
           + version()
           + ".\n"
           + " \n"
           + "If you click \"OK\", ImageJ will quit\n"
           + "and you will be running the upgraded\n"
           + "version after you restart ImageJ.\n";
   gd.addMessage(msg);
   gd.showDialog();
   if (gd.wasCanceled()) return -1;
   else return gd.getNextChoiceIndex();
 }
Beispiel #5
0
 // DICOM options
 void dicom() {
   GenericDialog gd = new GenericDialog("DICOM Options");
   gd.addCheckbox("Open as 32-bit float", Prefs.openDicomsAsFloat);
   // gd.addCheckbox("Calculate voxel depth", Prefs.calculateDicomVoxelDepth);
   gd.addMessage("Orthogonal Views");
   gd.setInsets(5, 40, 0);
   gd.addCheckbox("Rotate YZ", Prefs.rotateYZ);
   gd.setInsets(0, 40, 0);
   gd.addCheckbox("Flip XZ", Prefs.flipXZ);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   Prefs.openDicomsAsFloat = gd.getNextBoolean();
   // Prefs.calculateDicomVoxelDepth = gd.getNextBoolean();
   Prefs.rotateYZ = gd.getNextBoolean();
   Prefs.flipXZ = gd.getNextBoolean();
 }
Beispiel #6
0
  // Input/Output options
  void io() {
    GenericDialog gd = new GenericDialog("I/O Options");
    gd.addNumericField("JPEG quality (0-100):", FileSaver.getJpegQuality(), 0, 3, "");
    gd.addNumericField("GIF and PNG transparent index:", Prefs.getTransparentIndex(), 0, 3, "");
    gd.addStringField(
        "File extension for tables (.txt, .xls or .csv):", Prefs.get("options.ext", ".csv"), 4);
    gd.addCheckbox("Use JFileChooser to open/save", Prefs.useJFileChooser);
    if (!IJ.isMacOSX())
      gd.addCheckbox("Use_file chooser to import sequences", Prefs.useFileChooser);
    gd.addCheckbox("Save TIFF and raw in Intel byte order", Prefs.intelByteOrder);
    gd.addCheckbox("Skip dialog when opening .raw files", Prefs.skipRawDialog);

    gd.setInsets(15, 20, 0);
    gd.addMessage("Results Table Options");
    gd.setInsets(3, 40, 0);
    gd.addCheckbox("Copy_column headers", Prefs.copyColumnHeaders);
    gd.setInsets(0, 40, 0);
    gd.addCheckbox("Copy_row numbers", !Prefs.noRowNumbers);
    gd.setInsets(0, 40, 0);
    gd.addCheckbox("Save_column headers", !Prefs.dontSaveHeaders);
    gd.setInsets(0, 40, 0);
    gd.addCheckbox("Save_row numbers", !Prefs.dontSaveRowNumbers);

    gd.showDialog();
    if (gd.wasCanceled()) return;
    int quality = (int) gd.getNextNumber();
    if (quality < 0) quality = 0;
    if (quality > 100) quality = 100;
    FileSaver.setJpegQuality(quality);
    int transparentIndex = (int) gd.getNextNumber();
    Prefs.setTransparentIndex(transparentIndex);
    String extension = gd.getNextString();
    if (!extension.startsWith(".")) extension = "." + extension;
    Prefs.set("options.ext", extension);
    Prefs.useJFileChooser = gd.getNextBoolean();
    if (!IJ.isMacOSX()) Prefs.useFileChooser = gd.getNextBoolean();
    Prefs.intelByteOrder = gd.getNextBoolean();
    Prefs.skipRawDialog = gd.getNextBoolean();
    Prefs.copyColumnHeaders = gd.getNextBoolean();
    Prefs.noRowNumbers = !gd.getNextBoolean();
    Prefs.dontSaveHeaders = !gd.getNextBoolean();
    Prefs.dontSaveRowNumbers = !gd.getNextBoolean();
    return;
  }
Beispiel #7
0
 void checkForCalibrationConflict(ImagePlus imp, Calibration cal) {
   Calibration gcal = imp.getGlobalCalibration();
   if (gcal == null || !showConflictMessage || IJ.isMacro()) return;
   if (cal.pixelWidth == gcal.pixelWidth && cal.getUnit().equals(gcal.getUnit())) return;
   GenericDialog gd = new GenericDialog(imp.getTitle());
   gd.addMessage("The calibration of this image conflicts\nwith the current global calibration.");
   gd.addCheckbox("Disable_Global Calibration", true);
   gd.addCheckbox("Disable_these Messages", false);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   boolean disable = gd.getNextBoolean();
   if (disable) {
     imp.setGlobalCalibration(null);
     imp.setCalibration(cal);
     WindowManager.repaintImageWindows();
   }
   boolean dontShow = gd.getNextBoolean();
   if (dontShow) showConflictMessage = false;
 }
Beispiel #8
0
  public boolean analyseParticles(MyFunctions f) {
    GenericDialog gd = new GenericDialog("Analyse PALM/STORM Particles");
    gd.addNumericField("Minimum SNR", prefs.get("QuickPALM.snr", 5), 2);
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.fwhm", 4), 0);
    gd.addNumericField("Image plane pixel size (nm)", prefs.get("QuickPALM.pixelsize", 106), 2);
    gd.addCheckbox("Smart SNR", prefs.get("QuickPALM.smartsnr", true));
    gd.addCheckbox(
        "3D PALM (astigmatism) - will require calibration file",
        prefs.get("QuickPALM.is3d", false));
    gd.addCheckbox("Online rendering", prefs.get("QuickPALM.view", true));
    gd.addCheckbox("Attach to running acquisition", prefs.get("QuickPALM.attach", false));
    gd.addCheckbox("Stream particle info directly into file", prefs.get("QuickPALM.stream", true));
    gd.addMessage("\n");
    // -----------------------------------------
    gd.addMessage("-- Online rendering settings (used only if selected) --");
    gd.addMessage("\n");
    gd.addNumericField("Pixel size of rendered image (nm)", 30, 2);
    gd.addNumericField("Accumulate last (0 to accumulate all frames)", 0, 0);
    gd.addNumericField("Update every (frames)", 10, 0);
    // gd.addNumericField("Allow color saturation (%)", 50, 0);
    gd.addMessage("\n");
    // -----------------------------------------
    gd.addMessage("-- Attach to running acquisition settings (used only if selected) --");
    gd.addMessage("\n");
    gd.addStringField(
        "_Image name pattern (NN...NN represents the numerical change)",
        prefs.get("QuickPALM.pattern", "imgNNNNNNNNN.tif"),
        20);
    gd.addNumericField("Start NN...NN with", 0, 0);
    gd.addNumericField("In acquisition max. wait time for new image (ms)", 50, 0);
    gd.addMessage("\n");
    // -----------------------------------------
    gd.addMessage("-- Advanced settings (don't normally need to be changed) --");
    gd.addMessage("\n");
    gd.addNumericField("_Minimum symmetry (%)", prefs.get("QuickPALM.symmetry", 50), 0);
    gd.addNumericField(
        "Local threshold (% maximum intensity)", prefs.get("QuickPALM.lthreshold", 20), 0);
    gd.addNumericField("_Maximum iterations per frame", prefs.get("QuickPALM.maxiter", 1000), 0);
    gd.addNumericField(
        "Threads (each takes ~3*[frame size] in memory)", prefs.get("QuickPALM.nthreads", 50), 0);
    gd.addMessage("\n\nDon't forget to save the table in the end...");

    gd.showDialog();
    if (gd.wasCanceled()) return false;

    snr = (int) gd.getNextNumber();
    prefs.set("QuickPALM.snr", snr);
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.fwhm", fwhm);
    pixelsize = gd.getNextNumber();
    prefs.set("QuickPALM.pixelsize", pixelsize);

    smartsnr = gd.getNextBoolean();
    prefs.set("QuickPALM.smartsnr", smartsnr);
    is3d = gd.getNextBoolean();
    prefs.set("QuickPALM.is3d", is3d);
    view = gd.getNextBoolean();
    prefs.set("QuickPALM.view", view);
    attach = gd.getNextBoolean();
    prefs.set("QuickPALM.attach", attach);

    if (gd.getNextBoolean()) {
      f.psave = new ParticleSaver();
      f.psave.setup();
      prefs.set("QuickPALM.stream", true);
    } else prefs.set("QuickPALM.stream", false);
    // --

    magn = pixelsize / gd.getNextNumber();
    viewer_accumulate = (int) gd.getNextNumber();
    viewer_update = (int) gd.getNextNumber();

    // --
    pattern = gd.getNextString().trim();
    prefs.set("QuickPALM.pattern", pattern);
    prefix = pattern.substring(0, pattern.indexOf("N"));
    sufix = pattern.substring(pattern.lastIndexOf("N") + 1, pattern.length());
    nimchars = pattern.split("N").length - 1;
    nimstart = (int) gd.getNextNumber();
    waittime = (int) gd.getNextNumber();

    // --

    symmetry = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.symmetry", symmetry);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.lthreshold", pthrsh * 100);
    maxpart = (int) gd.getNextNumber();
    prefs.set("QuickPALM.maxiter", maxpart);
    threads = (int) gd.getNextNumber();
    prefs.set("QuickPALM.nthreads", threads);

    return true;
  }
Beispiel #9
0
  public boolean reconstructDataset() {

    view_modes[0] = "3D color";
    view_modes[1] = "2D histogram";
    view_modes[2] = "2D particle intensity (16-bit)";
    view_modes[3] = "2D particle intensity (8-bit)";

    GenericDialog gd = new GenericDialog("Reconstruct PALM/STORM Dataset");
    gd.addNumericField(
        "Target pixel size for the rendered image (nm)",
        prefs.get("QuickPALM.viewer_tpixelsize", 30),
        2);
    gd.addNumericField("Original image width (px)", prefs.get("QuickPALM.viewer_owidth", 512), 2);
    gd.addNumericField("Original image height (px)", prefs.get("QuickPALM.viewer_oheight", 512), 2);
    gd.addChoice("View mode", view_modes, prefs.get("QuickPALM.view_mode", view_modes[1]));
    // gd.addNumericField("Allow image saturation (%)", prefs.get("QuickPALM.saturation", 50), 0);

    gd.addCheckbox(
        "Simulate sub-difraction spot (gaussian convolution - only 2D)",
        prefs.get("QuickPALM.viewer_doConvolve", true));
    // gd.addCheckbox("Make 3D stack", prefs.get("QuickPALM.viewer_do3d", false));
    // gd.addCheckbox("Make movie", prefs.get("QuickPALM.viewer_doMovie", false));
    gd.addCheckbox("Make 3D stack", false);
    gd.addCheckbox("Make movie", false);
    // gd.addCheckbox("Save only and don't show", prefs.get("QuickPALM.viewer_doSave", false));
    gd.addMessage("\n");

    // -----------------------------------------
    gd.addMessage("-- Simulate sub-difraction spot settings (used only if selected) --");
    gd.addNumericField("FWHM of the spot", prefs.get("QuickPALM.viewer_fwhm", 30), 2);
    gd.addMessage("\n");

    // -----------------------------------------
    gd.addMessage("-- Make 3D stack settings (used only if selected) --");
    gd.addNumericField("Z-spacing between slices (nm)", prefs.get("QuickPALM.viewer_zstep", 50), 2);
    gd.addNumericField(
        "Merge particle Z-position above (nm - 0 for full Z range)",
        prefs.get("QuickPALM.viewer_mergeabove", 0),
        2);
    gd.addNumericField(
        "Merge particle Z-position bellow (nm - 0 for full Z range)",
        prefs.get("QuickPALM.viewer_mergebellow", 0),
        2);
    gd.addMessage("\n");

    // -----------------------------------------
    gd.addMessage("-- Make movie settings (used only if selected) --");
    gd.addNumericField(
        "Make a reconstruction in every N frames", prefs.get("QuickPALM.viewer_update", 10), 0);
    gd.addNumericField(
        "Accumulate N neighboring frames for each reconstruction\n(set to 0 to accumulate all the preceding frames)",
        prefs.get("QuickPALM.viewer_accumulate", 100),
        0);

    gd.showDialog();
    if (gd.wasCanceled()) return false;

    viewer_tpixelsize = gd.getNextNumber();
    prefs.set("QuickPALM.viewer_tpixelsize", viewer_tpixelsize);
    viewer_owidth = (int) gd.getNextNumber();
    prefs.set("QuickPALM.viewer_owidth", viewer_owidth);
    viewer_oheight = (int) gd.getNextNumber();
    prefs.set("QuickPALM.viewer_oheight", viewer_oheight);
    view_mode = gd.getNextChoice();
    prefs.set("QuickPALM.view_mode", view_mode);

    viewer_doConvolve = gd.getNextBoolean();
    prefs.set("QuickPALM.viewer_doConvolve", viewer_doConvolve);
    viewer_do3d = gd.getNextBoolean();
    prefs.set("QuickPALM.viewer_do3d", viewer_do3d);
    viewer_doMovie = gd.getNextBoolean();
    prefs.set("QuickPALM.viewer_doMovie", viewer_doMovie);
    // viewer_doSave = gd.getNextBoolean();
    // prefs.set("QuickPALM.viewer_doSave", viewer_doSave);

    // -- Simulate sub-difraction spot
    viewer_fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.viewer_fwhm", viewer_fwhm);

    // -- Show B&W
    // viewer_is8bit = gd.getNextBoolean();
    // prefs.set("QuickPALM.viewer_is8bit", viewer_is8bit);

    // -- Make 3D stack
    viewer_zstep = gd.getNextNumber();
    prefs.set("QuickPALM.viewer_zstep", viewer_zstep);
    viewer_mergeabove = gd.getNextNumber();
    prefs.set("QuickPALM.viewer_mergeabove", viewer_mergeabove);
    viewer_mergebellow = gd.getNextNumber();
    prefs.set("QuickPALM.viewer_mergebellow", viewer_mergebellow);

    // -- Make Movie
    viewer_update = (int) gd.getNextNumber();
    prefs.set("QuickPALM.viewer_update", viewer_update);
    viewer_accumulate = (int) gd.getNextNumber();
    prefs.set("QuickPALM.viewer_accumulate", viewer_accumulate);

    return true;
  }
  /** Ask for parameters and then execute. */
  public void run(String arg) {
    // 1 - Obtain the currently active image:
    ImagePlus imp = IJ.getImage();

    if (null == imp) {
      IJ.showMessage("There must be at least one image open");
      return;
    }

    if (imp.getBitDepth() != 8) {
      IJ.showMessage("Error", "Only 8-bit images are supported");
      return;
    }

    // 2 - Ask for parameters:
    GenericDialog gd = new GenericDialog("Auto Local Threshold");
    String[] methods = {
      "Try all",
      "Bernsen",
      "Contrast",
      "Mean",
      "Median",
      "MidGrey",
      "Niblack",
      "Otsu",
      "Phansalkar",
      "Sauvola"
    };
    gd.addMessage("Auto Local Threshold v1.5");
    gd.addChoice("Method", methods, methods[0]);
    gd.addNumericField("Radius", 15, 0);
    gd.addMessage("Special paramters (if different from default)");
    gd.addNumericField("Parameter_1", 0, 0);
    gd.addNumericField("Parameter_2", 0, 0);
    gd.addCheckbox("White objects on black background", true);
    if (imp.getStackSize() > 1) {
      gd.addCheckbox("Stack", false);
    }
    gd.addMessage("Thresholded result is always shown in white [255].");
    gd.showDialog();
    if (gd.wasCanceled()) return;

    // 3 - Retrieve parameters from the dialog
    String myMethod = gd.getNextChoice();
    int radius = (int) gd.getNextNumber();
    double par1 = (double) gd.getNextNumber();
    double par2 = (double) gd.getNextNumber();
    boolean doIwhite = gd.getNextBoolean();
    boolean doIstack = false;

    int stackSize = imp.getStackSize();
    if (stackSize > 1) doIstack = gd.getNextBoolean();

    // 4 - Execute!
    // long start = System.currentTimeMillis();
    if (myMethod.equals("Try all")) {
      ImageProcessor ip = imp.getProcessor();
      int xe = ip.getWidth();
      int ye = ip.getHeight();
      int ml = methods.length;
      ImagePlus imp2, imp3;
      ImageStack tstack = null, stackNew;
      if (stackSize > 1 && doIstack) {
        boolean doItAnyway = true;
        if (stackSize > 25) {
          YesNoCancelDialog d =
              new YesNoCancelDialog(
                  IJ.getInstance(),
                  "Auto Local Threshold",
                  "You might run out of memory.\n \nDisplay "
                      + stackSize
                      + " slices?\n \n \'No\' will process without display and\noutput results to the log window.");
          if (!d.yesPressed()) {
            //						doIlog=true; //will show in the log window
            doItAnyway = false;
          }
          if (d.cancelPressed()) return;
        }

        for (int j = 1; j <= stackSize; j++) {
          imp.setSlice(j);
          ip = imp.getProcessor();
          tstack = new ImageStack(xe, ye);
          for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
          imp2 = new ImagePlus("Auto Threshold", tstack);
          imp2.updateAndDraw();

          for (int k = 1; k < ml; k++) {
            imp2.setSlice(k);
            Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
          }
          // if (doItAnyway){
          CanvasResizer cr = new CanvasResizer();
          stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
          imp3 = new ImagePlus("Auto Threshold", stackNew);
          imp3.updateAndDraw();
          MontageMaker mm = new MontageMaker();
          mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); // 3 columns and 3 rows
        }
        imp.setSlice(1);
        // if (doItAnyway)
        IJ.run("Images to Stack", "method=[Copy (center)] title=Montage");
        return;
      } else { // single image try all
        tstack = new ImageStack(xe, ye);
        for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
        imp2 = new ImagePlus("Auto Threshold", tstack);
        imp2.updateAndDraw();

        for (int k = 1; k < ml; k++) {
          imp2.setSlice(k);
          // IJ.log("analyzing slice with "+methods[k]);
          Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
        }
        // imp2.setSlice(1);
        CanvasResizer cr = new CanvasResizer();
        stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
        imp3 = new ImagePlus("Auto Threshold", stackNew);
        imp3.updateAndDraw();
        MontageMaker mm = new MontageMaker();
        mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true);
        return;
      }
    } else { // selected a method
      if (stackSize > 1 && doIstack) { // whole stack
        //				if (doIstackHistogram) {// one global histogram
        //					Object[] result = exec(imp, myMethod, noWhite, noBlack, doIwhite, doIset, doIlog,
        // doIstackHistogram );
        //				}
        //				else{ // slice by slice
        for (int k = 1; k <= stackSize; k++) {
          imp.setSlice(k);
          Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
        }
        //				}
        imp.setSlice(1);
      } else { // just one slice
        Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
      }
      // 5 - If all went well, show the image:
      // not needed here as the source image is binarised
    }
  }
Beispiel #11
0
 public void itemStateChanged(ItemEvent e) {
   ImagePlus imp = WindowManager.getCurrentImage();
   if (imp == null) return;
   if (!imp.isComposite()) {
     int channels = imp.getNChannels();
     if (channels == 1 && imp.getStackSize() <= 4) channels = imp.getStackSize();
     if (imp.getBitDepth() == 24 || (channels > 1 && channels < CompositeImage.MAX_CHANNELS)) {
       GenericDialog gd = new GenericDialog(imp.getTitle());
       gd.addMessage("Convert to multi-channel composite image?");
       gd.showDialog();
       if (gd.wasCanceled()) return;
       else IJ.doCommand("Make Composite");
     } else {
       IJ.error(
           "Channels",
           "A composite image is required (e.g., "
               + moreLabel
               + " Open HeLa Cells),\nor create one using "
               + moreLabel
               + " Make Composite.");
       return;
     }
   }
   if (!imp.isComposite()) return;
   CompositeImage ci = (CompositeImage) imp;
   Object source = e.getSource();
   if (source == choice) {
     int index = ((Choice) source).getSelectedIndex();
     switch (index) {
       case 0:
         ci.setMode(IJ.COMPOSITE);
         break;
       case 1:
         ci.setMode(IJ.COLOR);
         break;
       case 2:
         ci.setMode(IJ.GRAYSCALE);
         break;
     }
     ci.updateAndDraw();
     if (Recorder.record) {
       String mode = null;
       switch (index) {
         case 0:
           mode = "composite";
           break;
         case 1:
           mode = "color";
           break;
         case 2:
           mode = "grayscale";
           break;
       }
       Recorder.record("Stack.setDisplayMode", mode);
     }
   } else if (source instanceof Checkbox) {
     for (int i = 0; i < checkbox.length; i++) {
       Checkbox cb = (Checkbox) source;
       if (cb == checkbox[i]) {
         if (ci.getMode() == IJ.COMPOSITE) {
           boolean[] active = ci.getActiveChannels();
           active[i] = cb.getState();
           if (Recorder.record) {
             String str = "";
             for (int c = 0; c < ci.getNChannels(); c++) str += active[c] ? "1" : "0";
             Recorder.record("Stack.setActiveChannels", str);
             Recorder.record("//Stack.toggleChannel", imp.getChannel());
           }
         } else {
           imp.setPosition(i + 1, imp.getSlice(), imp.getFrame());
           if (Recorder.record) Recorder.record("Stack.setChannel", i + 1);
         }
         ci.updateAndDraw();
         return;
       }
     }
   }
 }