public void run(ImageProcessor ip) {
if (enlarge && gd.wasOKed())
synchronized (this) {
if (!isEnlarged) {
enlargeCanvas();
isEnlarged = true;
}
}
if (isEnlarged) { // enlarging may have made the ImageProcessor invalid, also for the parallel
// threads
int slice = pfr.getSliceNumber();
if (imp.getStackSize() == 1) ip = imp.getProcessor();
else ip = imp.getStack().getProcessor(slice);
}
ip.setInterpolationMethod(interpolationMethod);
if (fillWithBackground) {
Color bgc = Toolbar.getBackgroundColor();
if (bitDepth == 8) ip.setBackgroundValue(ip.getBestIndex(bgc));
else if (bitDepth == 24) ip.setBackgroundValue(bgc.getRGB());
} else ip.setBackgroundValue(0);
ip.rotate(angle);
if (!gd.wasOKed()) drawGridLines(gridLines);
if (isEnlarged && imp.getStackSize() == 1) {
imp.changes = true;
imp.updateAndDraw();
Undo.setup(Undo.COMPOUND_FILTER_DONE, imp);
}
}
/**
* Builds dialog to query users for projection parameters.
*
* @param start starting slice to display
* @param stop last slice
*/
protected GenericDialog buildControlDialog(int start, int stop) {
GenericDialog gd = new GenericDialog("ZProjection", IJ.getInstance());
gd.addNumericField("Start slice:", startSlice, 0 /*digits*/);
gd.addNumericField("Stop slice:", stopSlice, 0 /*digits*/);
gd.addChoice("Projection type", METHODS, METHODS[method]);
if (isHyperstack && imp.getNFrames() > 1 && imp.getNSlices() > 1)
gd.addCheckbox("All time frames", allTimeFrames);
return gd;
}
public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) {
this.pfr = pfr;
String macroOptions = Macro.getOptions();
if (macroOptions != null) {
if (macroOptions.indexOf(" interpolate") != -1)
macroOptions.replaceAll(" interpolate", " interpolation=Bilinear");
else if (macroOptions.indexOf(" interpolation=") == -1)
macroOptions = macroOptions + " interpolation=None";
Macro.setOptions(macroOptions);
}
gd = new GenericDialog("Rotate", IJ.getInstance());
gd.addNumericField("Angle (degrees):", angle, (int) angle == angle ? 1 : 2);
gd.addNumericField("Grid Lines:", gridLines, 0);
gd.addChoice("Interpolation:", methods, methods[interpolationMethod]);
if (bitDepth == 8 || bitDepth == 24)
gd.addCheckbox("Fill with Background Color", fillWithBackground);
if (canEnlarge) gd.addCheckbox("Enlarge Image to Fit Result", enlarge);
else enlarge = false;
gd.addPreviewCheckbox(pfr);
gd.addDialogListener(this);
gd.showDialog();
drawGridLines(0);
if (gd.wasCanceled()) {
return DONE;
}
if (!enlarge) flags |= KEEP_PREVIEW; // standard filter without enlarge
else if (imp.getStackSize() == 1) flags |= NO_CHANGES; // undoable as a "compound filter"
return IJ.setupDialog(imp, flags);
}
void showDialog() {
int width = imp.getWidth();
int height = imp.getHeight();
Calibration cal = imp.getCalibration();
int places;
if (cal.scaled()) {
pixelWidth = cal.pixelWidth;
pixelHeight = cal.pixelHeight;
units = cal.getUnits();
places = 2;
} else {
pixelWidth = 1.0;
pixelHeight = 1.0;
units = "pixels";
places = 0;
}
if (areaPerPoint == 0.0)
areaPerPoint =
(width * cal.pixelWidth * height * cal.pixelHeight) / 81.0; // default to 9x9 grid
ImageWindow win = imp.getWindow();
GenericDialog gd = new GenericDialog("Grid...");
gd.addChoice("Grid Type:", types, type);
gd.addNumericField("Area per Point:", areaPerPoint, places, 6, units + "^2");
gd.addChoice("Color:", colors, color);
gd.addCheckbox("Random Offset", randomOffset);
gd.addDialogListener(this);
gd.showDialog();
if (gd.wasCanceled()) showGrid(null);
}
public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) {
iterations = (int) gd.getNextNumber();
count = (int) gd.getNextNumber();
boolean bb = Prefs.blackBackground;
Prefs.blackBackground = gd.getNextBoolean();
if (Prefs.blackBackground != bb) ThresholdAdjuster.update();
Prefs.padEdges = gd.getNextBoolean();
EDM.setOutputType(gd.getNextChoiceIndex());
boolean isInvalid = gd.invalidNumber();
if (iterations < 1) {
iterations = 1;
isInvalid = true;
}
if (iterations > MAX_ITERATIONS) {
iterations = MAX_ITERATIONS;
isInvalid = true;
}
if (count < 1) {
count = 1;
isInvalid = true;
}
if (count > 8) {
count = 8;
isInvalid = true;
}
if (isInvalid) return false;
if (imp != null) {
operation = gd.getNextChoice();
arg = operation.toLowerCase();
}
return true;
}
void interpolate() {
Roi roi = imp.getRoi();
if (roi == null) {
noRoi("Interpolate");
return;
}
if (roi.getType() == Roi.POINT) return;
if (IJ.isMacro() && Macro.getOptions() == null) Macro.setOptions("interval=1");
GenericDialog gd = new GenericDialog("Interpolate");
gd.addNumericField("Interval:", 1.0, 1, 4, "pixel");
gd.addCheckbox("Smooth", IJ.isMacro() ? false : smooth);
gd.showDialog();
if (gd.wasCanceled()) return;
double interval = gd.getNextNumber();
smooth = gd.getNextBoolean();
Undo.setup(Undo.ROI, imp);
FloatPolygon poly = roi.getInterpolatedPolygon(interval, smooth);
int t = roi.getType();
int type = roi.isLine() ? Roi.FREELINE : Roi.FREEROI;
if (t == Roi.POLYGON && interval > 1.0) type = Roi.POLYGON;
if ((t == Roi.RECTANGLE || t == Roi.OVAL || t == Roi.FREEROI) && interval >= 5.0)
type = Roi.POLYGON;
if ((t == Roi.LINE || t == Roi.FREELINE) && interval >= 5.0) type = Roi.POLYLINE;
if (t == Roi.POLYLINE && interval >= 1.0) type = Roi.POLYLINE;
ImageCanvas ic = imp.getCanvas();
if (poly.npoints <= 150 && ic != null && ic.getMagnification() >= 12.0)
type = roi.isLine() ? Roi.POLYLINE : Roi.POLYGON;
Roi p = new PolygonRoi(poly, type);
if (roi.getStroke() != null) p.setStrokeWidth(roi.getStrokeWidth());
p.setStrokeColor(roi.getStrokeColor());
p.setName(roi.getName());
transferProperties(roi, p);
imp.setRoi(p);
}
public void run(String arg) {
int[] wList = WindowManager.getIDList();
if (wList == null) {
IJ.error("No images are open.");
return;
}
double thalf = 0.5;
boolean keep;
GenericDialog gd = new GenericDialog("Bleach correction");
gd.addNumericField("t½:", thalf, 1);
gd.addCheckbox("Keep source stack:", true);
gd.showDialog();
if (gd.wasCanceled()) return;
long start = System.currentTimeMillis();
thalf = gd.getNextNumber();
keep = gd.getNextBoolean();
if (keep) IJ.run("Duplicate...", "title='Bleach corrected' duplicate");
ImagePlus imp1 = WindowManager.getCurrentImage();
int d1 = imp1.getStackSize();
double v1, v2;
int width = imp1.getWidth();
int height = imp1.getHeight();
ImageProcessor ip1, ip2, ip3;
int slices = imp1.getStackSize();
ImageStack stack1 = imp1.getStack();
ImageStack stack2 = imp1.getStack();
int currentSlice = imp1.getCurrentSlice();
for (int n = 1; n <= slices; n++) {
ip1 = stack1.getProcessor(n);
ip3 = stack1.getProcessor(1);
ip2 = stack2.getProcessor(n);
for (int x = 0; x < width; x++) {
for (int y = 0; y < height; y++) {
v1 = ip1.getPixelValue(x, y);
v2 = ip3.getPixelValue(x, y);
// =B8/(EXP(-C$7*A8))
v1 = (v1 / Math.exp(-n * thalf));
ip2.putPixelValue(x, y, v1);
}
}
IJ.showProgress((double) n / slices);
IJ.showStatus(n + "/" + slices);
}
// stack2.show();
imp1.updateAndDraw();
}
public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) {
checkc2 = gd.getNextBoolean();
double[] params = new double[10];
int[] fixes = new int[10];
for (int i = 0; i < params.length; i++) {
params[i] = gd.getNextNumber();
if (gd.getNextBoolean()) {
fixes[i] = 1;
} else {
fixes[i] = 0;
}
}
gd.getNextBoolean();
gd.getNextNumber();
gd.getNextNumber();
NLLSfit_v2 fitclass = new NLLSfit_v2(this, 0);
double[] stats = new double[2];
float[] fit = fitclass.fitdata(params, fixes, null, tempdata, null, stats, true);
pw.updateSeries(fit, series, false);
c2 = (float) stats[1];
return true;
}
public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) {
angle = gd.getNextNumber();
// only check for invalid input to "angle", don't care about gridLines
if (gd.invalidNumber()) {
if (gd.wasOKed()) IJ.error("Angle is invalid.");
return false;
}
gridLines = (int) gd.getNextNumber();
interpolationMethod = gd.getNextChoiceIndex();
if (bitDepth == 8 || bitDepth == 24) fillWithBackground = gd.getNextBoolean();
if (canEnlarge) enlarge = gd.getNextBoolean();
return true;
}
void addSelection() {
ImagePlus imp = IJ.getImage();
String macroOptions = Macro.getOptions();
if (macroOptions != null && IJ.macroRunning() && macroOptions.indexOf("remove") != -1) {
imp.setOverlay(null);
return;
}
Roi roi = imp.getRoi();
if (roi == null && imp.getOverlay() != null) {
GenericDialog gd = new GenericDialog("No Selection");
gd.addMessage("\"Overlay>Add\" requires a selection.");
gd.setInsets(15, 40, 0);
gd.addCheckbox("Remove existing overlay", false);
gd.showDialog();
if (gd.wasCanceled()) return;
if (gd.getNextBoolean()) imp.setOverlay(null);
return;
}
if (roi == null) {
IJ.error("This command requires a selection.");
return;
}
roi = (Roi) roi.clone();
if (roi.getStrokeColor() == null) roi.setStrokeColor(Toolbar.getForegroundColor());
int width = Line.getWidth();
Rectangle bounds = roi.getBounds();
boolean tooWide = width > Math.max(bounds.width, bounds.height) / 3.0;
if (roi.getStroke() == null && width > 1 && !tooWide) roi.setStrokeWidth(Line.getWidth());
Overlay overlay = imp.getOverlay();
if (overlay != null && overlay.size() > 0 && !roi.isDrawingTool()) {
Roi roi2 = overlay.get(overlay.size() - 1);
if (roi.getStroke() == null) roi.setStrokeWidth(roi2.getStrokeWidth());
if (roi.getFillColor() == null) roi.setFillColor(roi2.getFillColor());
}
boolean points = roi instanceof PointRoi && ((PolygonRoi) roi).getNCoordinates() > 1;
if (points) roi.setStrokeColor(Color.red);
if (!IJ.altKeyDown() && !(roi instanceof Arrow)) {
RoiProperties rp = new RoiProperties("Add to Overlay", roi);
if (!rp.showDialog()) return;
}
String name = roi.getName();
boolean newOverlay = name != null && name.equals("new-overlay");
if (overlay == null || newOverlay) overlay = new Overlay();
overlay.add(roi);
imp.setOverlay(overlay);
overlay2 = overlay;
if (points || (roi instanceof ImageRoi) || (roi instanceof Arrow)) imp.killRoi();
Undo.setup(Undo.OVERLAY_ADDITION, imp);
}
public boolean showDialog(ImageStack stack) {
hyperstack = imp.isHyperStack();
boolean showCheckbox = false;
if (hyperstack && imp.getNSlices() > 1 && imp.getNFrames() > 1) showCheckbox = true;
else if (hyperstack && imp.getNSlices() > 1) reduceSlices = true;
int n = stack.getSize();
GenericDialog gd = new GenericDialog("Reduce Size");
gd.addNumericField("Reduction Factor:", factor, 0);
if (showCheckbox) gd.addCheckbox("Reduce in Z-Dimension", false);
gd.showDialog();
if (gd.wasCanceled()) return false;
factor = (int) gd.getNextNumber();
if (showCheckbox) reduceSlices = gd.getNextBoolean();
return true;
}
void checkForCalibrationConflict(ImagePlus imp, Calibration cal) {
Calibration gcal = imp.getGlobalCalibration();
if (gcal == null || !showConflictMessage || IJ.isMacro()) return;
if (cal.pixelWidth == gcal.pixelWidth && cal.getUnit().equals(gcal.getUnit())) return;
GenericDialog gd = new GenericDialog(imp.getTitle());
gd.addMessage("The calibration of this image conflicts\nwith the current global calibration.");
gd.addCheckbox("Disable_Global Calibration", true);
gd.addCheckbox("Disable_these Messages", false);
gd.showDialog();
if (gd.wasCanceled()) return;
boolean disable = gd.getNextBoolean();
if (disable) {
imp.setGlobalCalibration(null);
imp.setCalibration(cal);
WindowManager.repaintImageWindows();
}
boolean dontShow = gd.getNextBoolean();
if (dontShow) showConflictMessage = false;
}
public void run(String arg) {
GenericDialog gd = new GenericDialog("Options");
gd.addCheckbox("Add_Titles", true);
gd.addCheckbox("Close Original", false);
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
boolean addtitles = gd.getNextBoolean();
boolean closeorig = gd.getNextBoolean();
// first get the table window
Frame[] niframes = WindowManager.getNonImageWindows();
boolean first = true;
TextWindow tw2 = null;
int ncols = 0;
String[] col_labels = null;
for (int i = 0; i < niframes.length; i++) {
if (niframes[i] instanceof TextWindow && !niframes[i].getTitle().equals("Log")) {
TextWindow tw = (TextWindow) niframes[i];
TextPanel tp = tw.getTextPanel();
List<List<String>> listtable = table_tools.table2listtable(tp);
if (listtable.size() == 0) {
col_labels = table_tools.getcollabels(tp);
ncols = col_labels.length;
ArrayList<String> temp = new ArrayList<String>();
for (int j = 0; j < ncols; j++) temp.add("");
listtable.add(temp);
}
if (first) {
col_labels = table_tools.getcollabels(tp);
ncols = col_labels.length;
String headings = tp.getColumnHeadings();
if (addtitles) {
String[] titles = repeated(tw.getTitle(), listtable.size());
table_tools.add_listtable_column(listtable, titles, 0);
headings = "name\t" + headings;
}
tw2 =
new TextWindow(
"Combined Table", headings, table_tools.print_listtable(listtable), 400, 200);
first = false;
} else {
if (addtitles) {
String[] titles = repeated(tw.getTitle(), listtable.size());
table_tools.add_listtable_column(listtable, titles, 0);
}
tw2.append(table_tools.print_listtable(listtable));
}
if (closeorig) tw.close();
}
}
}
public void run(String arg) {
ImagePlus imp = WindowManager.getCurrentImage();
if (imp == null) {
IJ.noImage();
return;
}
if (imp.getStackSize() > 1) {
IJ.error("This command requires a montage");
return;
}
GenericDialog gd = new GenericDialog("Stack Maker");
gd.addNumericField("Images_per_row: ", w, 0);
gd.addNumericField("Images_per_column: ", h, 0);
gd.addNumericField("Border width: ", b, 0);
gd.showDialog();
if (gd.wasCanceled()) return;
w = (int) gd.getNextNumber();
h = (int) gd.getNextNumber();
b = (int) gd.getNextNumber();
ImageStack stack = makeStack(imp.getProcessor(), w, h, b);
new ImagePlus("Stack", stack).show();
}
int showDialog(String[] versions) {
GenericDialog gd = new GenericDialog("ImageJ Updater");
gd.addChoice("Upgrade To:", versions, versions[0]);
String msg =
"You are currently running v"
+ version()
+ ".\n"
+ " \n"
+ "If you click \"OK\", ImageJ will quit\n"
+ "and you will be running the upgraded\n"
+ "version after you restart ImageJ.\n";
gd.addMessage(msg);
gd.showDialog();
if (gd.wasCanceled()) return -1;
else return gd.getNextChoiceIndex();
}
// Conversion Options
void conversions() {
double[] weights = ColorProcessor.getWeightingFactors();
boolean weighted = !(weights[0] == 1d / 3d && weights[1] == 1d / 3d && weights[2] == 1d / 3d);
// boolean weighted = !(Math.abs(weights[0]-1d/3d)<0.0001 && Math.abs(weights[1]-1d/3d)<0.0001
// && Math.abs(weights[2]-1d/3d)<0.0001);
GenericDialog gd = new GenericDialog("Conversion Options");
gd.addCheckbox("Scale when converting", ImageConverter.getDoScaling());
String prompt = "Weighted RGB conversions";
if (weighted)
prompt +=
" (" + IJ.d2s(weights[0]) + "," + IJ.d2s(weights[1]) + "," + IJ.d2s(weights[2]) + ")";
gd.addCheckbox(prompt, weighted);
gd.showDialog();
if (gd.wasCanceled()) return;
ImageConverter.setDoScaling(gd.getNextBoolean());
Prefs.weightedColor = gd.getNextBoolean();
if (!Prefs.weightedColor) ColorProcessor.setWeightingFactors(1d / 3d, 1d / 3d, 1d / 3d);
else if (Prefs.weightedColor && !weighted)
ColorProcessor.setWeightingFactors(0.299, 0.587, 0.114);
return;
}
public double[][] get_constraints(double[] params) {
// here we populate the constraints
GenericDialog gd = new GenericDialog("Constraints");
for (int i = 0; i < 10; i++) {
if (constraints == null) {
gd.addNumericField("P" + (i + 1) + "_upper", params[i], 5, 10, null);
gd.addNumericField("P" + (i + 1) + "_lower", params[i], 5, 10, null);
} else {
gd.addNumericField("P" + (i + 1) + "_upper", constraints[1][i], 5, 10, null);
gd.addNumericField("P" + (i + 1) + "_lower", constraints[0][i], 5, 10, null);
}
}
gd.showDialog();
if (gd.wasCanceled()) {
return null;
}
double[][] constraints = new double[2][10];
for (int i = 0; i < 10; i++) {
constraints[1][i] = gd.getNextNumber();
constraints[0][i] = gd.getNextNumber();
}
return constraints;
}
public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) {
int width = imp.getWidth();
int height = imp.getHeight();
type = gd.getNextChoice();
areaPerPoint = gd.getNextNumber();
color = gd.getNextChoice();
randomOffset = gd.getNextBoolean();
double minArea = (width * height) / 50000.0;
if (type.equals(types[1]) && minArea < 144.0) minArea = 144.0;
else if (minArea < 16) minArea = 16.0;
if (areaPerPoint / (pixelWidth * pixelHeight) < minArea) {
String err = "\"Area per Point\" too small";
if (gd.wasOKed()) IJ.error("Grid", err);
else IJ.showStatus(err);
return true;
}
double tileSize = Math.sqrt(areaPerPoint);
tileWidth = tileSize / pixelWidth;
tileHeight = tileSize / pixelHeight;
if (randomOffset) {
xstart = (int) (random.nextDouble() * tileWidth);
ystart = (int) (random.nextDouble() * tileHeight);
} else {
xstart = (int) (tileWidth / 2.0 + 0.5);
ystart = (int) (tileHeight / 2.0 + 0.5);
}
linesV = (int) ((width - xstart) / tileWidth) + 1;
linesH = (int) ((height - ystart) / tileHeight) + 1;
if (gd.invalidNumber()) return true;
if (type.equals(types[0])) drawLines();
else if (type.equals(types[1])) drawCrosses();
else if (type.equals(types[2])) drawPoints();
else showGrid(null);
return true;
}
private void makeBand(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi == null) {
noRoi("Make Band");
return;
}
if (!roi.isArea()) {
IJ.error("Make Band", "Area selection required");
return;
}
Calibration cal = imp.getCalibration();
double pixels = bandSize;
double size = pixels * cal.pixelWidth;
int decimalPlaces = 0;
if ((int) size != size) decimalPlaces = 2;
GenericDialog gd = new GenericDialog("Make Band");
gd.addNumericField("Band Size:", size, decimalPlaces, 4, cal.getUnits());
gd.showDialog();
if (gd.wasCanceled()) return;
size = gd.getNextNumber();
if (Double.isNaN(size)) {
IJ.error("Make Band", "invalid number");
return;
}
int n = (int) Math.round(size / cal.pixelWidth);
if (n > 255) {
IJ.error("Make Band", "Cannot make bands wider that 255 pixels");
return;
}
int width = imp.getWidth();
int height = imp.getHeight();
Rectangle r = roi.getBounds();
ImageProcessor ip = roi.getMask();
if (ip == null) {
ip = new ByteProcessor(r.width, r.height);
ip.invert();
}
ImageProcessor mask = new ByteProcessor(width, height);
mask.insert(ip, r.x, r.y);
ImagePlus edm = new ImagePlus("mask", mask);
boolean saveBlackBackground = Prefs.blackBackground;
Prefs.blackBackground = false;
IJ.run(edm, "Distance Map", "");
Prefs.blackBackground = saveBlackBackground;
ip = edm.getProcessor();
ip.setThreshold(0, n, ImageProcessor.NO_LUT_UPDATE);
int xx = -1, yy = -1;
for (int x = r.x; x < r.x + r.width; x++) {
for (int y = r.y; y < r.y + r.height; y++) {
if (ip.getPixel(x, y) < n) {
xx = x;
yy = y;
break;
}
}
if (xx >= 0 || yy >= 0) break;
}
int count = IJ.doWand(edm, xx, yy, 0, null);
if (count <= 0) {
IJ.error("Make Band", "Unable to make band");
return;
}
ShapeRoi roi2 = new ShapeRoi(edm.getRoi());
if (!(roi instanceof ShapeRoi)) roi = new ShapeRoi(roi);
ShapeRoi roi1 = (ShapeRoi) roi;
roi2 = roi2.not(roi1);
imp.setRoi(roi2);
bandSize = n;
}
void addImage() {
ImagePlus imp = IJ.getImage();
int[] wList = WindowManager.getIDList();
if (wList == null || wList.length < 2) {
IJ.error("Add Image...", "The command requires at least two open images.");
return;
}
String[] titles = new String[wList.length];
for (int i = 0; i < wList.length; i++) {
ImagePlus imp2 = WindowManager.getImage(wList[i]);
titles[i] = imp2 != null ? imp2.getTitle() : "";
}
int x = 0, y = 0;
Roi roi = imp.getRoi();
if (roi != null && roi.isArea()) {
Rectangle r = roi.getBounds();
x = r.x;
y = r.y;
}
int index = 0;
if (wList.length == 2) {
ImagePlus i1 = WindowManager.getImage(wList[0]);
ImagePlus i2 = WindowManager.getImage(wList[1]);
if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) index = 1;
} else if (imp.getID() == wList[0]) index = 1;
GenericDialog gd = new GenericDialog("Add Image...");
gd.addChoice("Image to add:", titles, titles[index]);
gd.addNumericField("X location:", x, 0);
gd.addNumericField("Y location:", y, 0);
gd.addNumericField("Opacity (0-100%):", 100, 0);
gd.addCheckbox("Create image selection", createImageRoi);
gd.showDialog();
if (gd.wasCanceled()) return;
index = gd.getNextChoiceIndex();
x = (int) gd.getNextNumber();
y = (int) gd.getNextNumber();
double opacity = gd.getNextNumber() / 100.0;
createImageRoi = gd.getNextBoolean();
ImagePlus overlay = WindowManager.getImage(wList[index]);
if (wList.length == 2) {
ImagePlus i1 = WindowManager.getImage(wList[0]);
ImagePlus i2 = WindowManager.getImage(wList[1]);
if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) {
imp = i1;
overlay = i2;
}
}
if (overlay == imp) {
IJ.error(
"Add Image...", "Image to be added cannot be the same as\n\"" + imp.getTitle() + "\".");
return;
}
if (overlay.getWidth() > imp.getWidth() && overlay.getHeight() > imp.getHeight()) {
IJ.error(
"Add Image...", "Image to be added cannnot be larger than\n\"" + imp.getTitle() + "\".");
return;
}
if (createImageRoi && x == 0 && y == 0) {
x = imp.getWidth() / 2 - overlay.getWidth() / 2;
y = imp.getHeight() / 2 - overlay.getHeight() / 2;
}
roi = new ImageRoi(x, y, overlay.getProcessor());
roi.setName(overlay.getShortTitle());
if (opacity != 1.0) ((ImageRoi) roi).setOpacity(opacity);
if (createImageRoi) imp.setRoi(roi);
else {
Overlay overlayList = imp.getOverlay();
if (overlayList == null) overlayList = new Overlay();
overlayList.add(roi);
imp.setOverlay(overlayList);
overlay2 = overlayList;
Undo.setup(Undo.OVERLAY_ADDITION, imp);
}
}
public void run(String arg) {
GenericDialog gd = new GenericDialog("Options");
double sfreq = 20000.0;
gd.addNumericField("Sampling Frequency?", sfreq, 1, 10, null);
String[] psfchoice = {"3D Gaussian", "Gaus-Lorentz^2", "2D Gaussian"};
gd.addChoice("PSF Type?", psfchoice, psfchoice[0]);
String[] filetypechoice = {
"Confocor 3 raw", "Short binary trajectory", "PlotWindow trajectory", "Ascii Text File"
};
gd.addChoice("File Type?", filetypechoice, filetypechoice[0]);
boolean ch2green = true;
gd.addCheckbox("Ch2 is green?", ch2green);
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
sfreq = gd.getNextNumber();
int psfflag = gd.getNextChoiceIndex();
int fileflag = gd.getNextChoiceIndex();
ch2green = gd.getNextBoolean();
int nfiles = 0;
Object[] histograms = null;
int xmax = 0;
int ymax = 0;
String[] names = null;
if (fileflag < 2) {
jdataio ioclass = new jdataio();
File[] filearray = ioclass.openfiles(OpenDialog.getDefaultDirectory(), IJ.getInstance());
if (filearray.length == 0) {
return;
}
String dir = filearray[0].getAbsolutePath();
int sepindex = dir.lastIndexOf(File.separator);
String newdir = dir.substring(0, sepindex + 1);
OpenDialog.setDefaultDirectory(newdir);
nfiles = filearray.length / 2;
if (nfiles > 25) {
nfiles = 25;
}
histograms = new Object[nfiles];
names = organize_c3_files(filearray);
for (int i = 0; i < nfiles; i++) {
try {
int length1 = (int) (((double) filearray[2 * i].length() - 128.0) / 4.0);
int length2 = (int) (((double) filearray[2 * i + 1].length() - 128.0) / 4.0);
int length3 = (int) (((double) filearray[2 * i].length()) / 2.0);
int length4 = (int) (((double) filearray[2 * i + 1].length()) / 2.0);
InputStream instream = new BufferedInputStream(new FileInputStream(filearray[2 * i]));
InputStream instream2 =
new BufferedInputStream(new FileInputStream(filearray[2 * i + 1]));
if (fileflag == 0) {
int[] pmdata = new int[length1];
int[] pmdata2 = new int[length2];
if (!ioclass.skipstreambytes(instream, 128)) {
showioerror();
instream.close();
return;
}
if (!ioclass.skipstreambytes(instream2, 128)) {
showioerror();
instream2.close();
return;
}
if (!ioclass.readintelintfile(instream, length1, pmdata)) {
showioerror();
instream.close();
return;
}
if (!ioclass.readintelintfile(instream2, length2, pmdata2)) {
showioerror();
instream2.close();
return;
}
if (ch2green) {
histograms[i] = (new pmodeconvert()).pm2pch(pmdata2, pmdata, sfreq, 20000000);
} else {
histograms[i] = (new pmodeconvert()).pm2pch(pmdata, pmdata2, sfreq, 20000000);
}
} else {
float[] tmdata = new float[length3];
float[] tmdata2 = new float[length4];
if (!ioclass.readintelshortfile(instream, length3, tmdata)) {
showioerror();
instream.close();
return;
}
if (!ioclass.readintelshortfile(instream2, length4, tmdata2)) {
showioerror();
instream2.close();
return;
}
if (ch2green) {
histograms[i] = (new pmodeconvert()).create_2Dhistogram(tmdata2, tmdata);
} else {
histograms[i] = (new pmodeconvert()).create_2Dhistogram(tmdata, tmdata2);
}
}
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
instream.close();
instream2.close();
} catch (IOException e) {
showioerror();
return;
}
}
} else {
if (fileflag == 2) {
ImageWindow iw = WindowManager.getCurrentWindow();
float[][] trajectories = (float[][]) jutils.runPW4VoidMethod(iw, "getYValues");
float[][] tempxvals = (float[][]) jutils.runPW4VoidMethod(iw, "getXValues");
sfreq = 1.0 / ((double) tempxvals[0][1]);
nfiles = trajectories.length / 2;
if (nfiles > 25) {
nfiles = 25;
}
names = new String[nfiles + 1];
names[nfiles] = "avg";
histograms = new Object[nfiles];
for (int i = 0; i < nfiles; i++) {
names[i] = "trajectory " + (i + 1);
if (ch2green) {
histograms[i] =
(new pmodeconvert())
.create_2Dhistogram(trajectories[2 * i + 1], trajectories[2 * i]);
} else {
histograms[i] =
(new pmodeconvert())
.create_2Dhistogram(trajectories[2 * i], trajectories[2 * i + 1]);
}
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
}
} else {
// here we read tab delimited lines from files
jdataio ioclass = new jdataio();
File[] filearray = ioclass.openfiles(OpenDialog.getDefaultDirectory(), IJ.getInstance());
if (filearray.length == 0) {
return;
}
String dir = filearray[0].getAbsolutePath();
int sepindex = dir.lastIndexOf(File.separator);
String newdir = dir.substring(0, sepindex + 1);
OpenDialog.setDefaultDirectory(newdir);
nfiles = filearray.length;
if (nfiles > 25) {
nfiles = 25;
}
histograms = new Object[nfiles];
names = new String[nfiles + 1];
names[nfiles] = "avg";
for (int i = 0; i < nfiles; i++) {
try {
names[i] = filearray[i].getName();
BufferedReader d = new BufferedReader(new FileReader(filearray[i]));
String[] lines = new String[256];
int counter = 0;
do {
lines[counter] = d.readLine();
counter++;
} while ((lines[counter - 1] != null && lines[counter - 1] != "") && counter < 256);
int numcolumns = 0;
for (int j = 0; j < counter - 1; j++) {
int temp = getncolumns(lines[j]);
if (temp > numcolumns) {
numcolumns = temp;
}
}
float[][] temphist2 = null;
if (ch2green) {
temphist2 = new float[numcolumns][counter - 1];
} else {
temphist2 = new float[counter - 1][numcolumns];
}
for (int k = 0; k < counter - 1; k++) {
float[] temp = tab_delim2float(lines[k]);
for (int j = 0; j < numcolumns; j++) {
if (ch2green) {
temphist2[j][k] = temp[j];
} else {
temphist2[k][j] = temp[j];
}
}
}
histograms[i] = temphist2;
d.close();
} catch (IOException e) {
showioerror();
return;
}
}
for (int i = 0; i < nfiles; i++) {
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
}
}
}
// note that here x is green and y is red
float[][][] pch = new float[nfiles][xmax][ymax];
for (int i = 0; i < nfiles; i++) {
for (int j = 0; j < ((float[][]) histograms[i]).length; j++) {
for (int k = 0; k < ((float[][]) histograms[i])[j].length; k++) {
pch[i][j][k] = ((float[][]) histograms[i])[j][k];
}
}
}
final PCH2DFitWindow cw = new PCH2DFitWindow();
cw.init(names, pch, psfflag);
final Frame f = new Frame("PCH 2D Analysis");
f.setLocation(10, 10);
f.addWindowListener(
new WindowAdapter() {
public void windowClosing(WindowEvent e) {
f.dispose();
}
});
f.add(cw);
f.pack();
f.setResizable(false);
Insets ins = f.getInsets();
cw.totalSize.height = PCH2DFitWindow.H + ins.bottom + ins.top + 65;
cw.totalSize.width = PCH2DFitWindow.WR + ins.left + ins.right;
f.setSize(cw.totalSize);
f.setVisible(true);
cw.requestFocus();
}
public boolean get_errors(double[] params, int[] fixes) {
GenericDialog gd = new GenericDialog("Error Options");
String[] methods = {"Support Plane", "Monte Carlo"};
gd.addChoice("Method", methods, methods[0]);
float conf = 0.67f;
gd.addNumericField("SP_Confidence Limit (%)", (int) (conf * 100.0f), 5, 10, null);
String[] labels = {"P1", "P2", "P3", "P4", "P5", "P6", "P7", "P8", "P9", "P10"};
gd.addChoice("SP_Parameter", labels, labels[0]);
double spacing = 0.01;
gd.addNumericField("SP_Chi^2_plot_spacing (% of value)?", spacing * 100.0, 2, 10, null);
int ntrials = 100;
gd.addNumericField("MC_#_Trials", ntrials, 0);
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
int methodindex = gd.getNextChoiceIndex();
conf = 0.01f * (float) gd.getNextNumber();
int paramindex = gd.getNextChoiceIndex();
spacing = 0.01 * gd.getNextNumber();
ntrials = (int) gd.getNextNumber();
if (methodindex == 0) {
support_plane_errors_v2 erclass = new support_plane_errors_v2(this, 0.0001, 50, false, 0.1);
int errindex = paramindex;
int nfit = 0;
for (int i = 0; i < labels.length; i++) {
if (fixes[i] == 0) {
nfit++;
}
}
int npts = tempdata.length;
int dofnum = npts - (nfit - 1) - 1;
int dofden = npts - nfit - 1;
double flim = (new jdist()).FLimit(dofnum, dofden, (double) conf);
IJ.log("FLimit = " + (float) flim);
if (flim == Double.NaN && flim < 1.0) {
IJ.showMessage("Invalid Limiting F Value");
return false;
}
double truespacing = Math.abs(params[errindex] * spacing);
double[][] c2plot =
erclass.geterrors(
params, fixes, constraints, tempdata, weights, flim, truespacing, errindex);
IJ.log("upper limit = " + c2plot[1][0] + " lower limit = " + c2plot[0][0]);
IJ.log(
"upper error = "
+ (c2plot[1][0] - params[errindex])
+ " lower error = "
+ (params[errindex] - c2plot[0][0]));
int templength = c2plot[0].length;
float[][] c2plotf = new float[2][templength - 1];
for (int i = 0; i < (templength - 1); i++) {
c2plotf[0][i] = (float) c2plot[0][i + 1];
c2plotf[1][i] = (float) c2plot[1][i + 1];
}
new PlotWindow4("c2 plot", labels[errindex], "Chi^2", c2plotf[0], c2plotf[1]).draw();
} else {
StringBuffer sb = new StringBuffer();
sb.append("Trial\t");
for (int i = 0; i < labels.length; i++) {
if (fixes[i] == 0) sb.append(labels[i] + "\t");
}
sb.append("chi^2");
tw = new TextWindow("Monte Carlo Results", sb.toString(), "", 400, 400);
redirect = true;
monte_carlo_errors_v2 erclass = new monte_carlo_errors_v2(this, 0.0001, 50, false, 0.1);
double[][] errors = erclass.geterrors(params, fixes, constraints, tempdata, weights, ntrials);
sb = new StringBuffer();
sb.append("StDev\t");
for (int i = 0; i < errors.length; i++) {
float[] ferr = new float[errors[0].length];
for (int j = 0; j < ferr.length; j++) ferr[j] = (float) errors[i][j];
float stdev = jstatistics.getstatistic("StDev", ferr, null);
sb.append("" + stdev);
if (i < (errors.length - 1)) sb.append("\t");
}
tw.append(sb.toString());
redirect = false;
}
return true;
}
// Input/Output options
void io() {
GenericDialog gd = new GenericDialog("I/O Options");
gd.addNumericField("JPEG quality (0-100):", FileSaver.getJpegQuality(), 0, 3, "");
gd.addNumericField("GIF and PNG transparent index:", Prefs.getTransparentIndex(), 0, 3, "");
gd.addStringField(
"File extension for tables (.txt, .xls or .csv):", Prefs.get("options.ext", ".csv"), 4);
gd.addCheckbox("Use JFileChooser to open/save", Prefs.useJFileChooser);
if (!IJ.isMacOSX())
gd.addCheckbox("Use_file chooser to import sequences", Prefs.useFileChooser);
gd.addCheckbox("Save TIFF and raw in Intel byte order", Prefs.intelByteOrder);
gd.addCheckbox("Skip dialog when opening .raw files", Prefs.skipRawDialog);
gd.setInsets(15, 20, 0);
gd.addMessage("Results Table Options");
gd.setInsets(3, 40, 0);
gd.addCheckbox("Copy_column headers", Prefs.copyColumnHeaders);
gd.setInsets(0, 40, 0);
gd.addCheckbox("Copy_row numbers", !Prefs.noRowNumbers);
gd.setInsets(0, 40, 0);
gd.addCheckbox("Save_column headers", !Prefs.dontSaveHeaders);
gd.setInsets(0, 40, 0);
gd.addCheckbox("Save_row numbers", !Prefs.dontSaveRowNumbers);
gd.showDialog();
if (gd.wasCanceled()) return;
int quality = (int) gd.getNextNumber();
if (quality < 0) quality = 0;
if (quality > 100) quality = 100;
FileSaver.setJpegQuality(quality);
int transparentIndex = (int) gd.getNextNumber();
Prefs.setTransparentIndex(transparentIndex);
String extension = gd.getNextString();
if (!extension.startsWith(".")) extension = "." + extension;
Prefs.set("options.ext", extension);
Prefs.useJFileChooser = gd.getNextBoolean();
if (!IJ.isMacOSX()) Prefs.useFileChooser = gd.getNextBoolean();
Prefs.intelByteOrder = gd.getNextBoolean();
Prefs.skipRawDialog = gd.getNextBoolean();
Prefs.copyColumnHeaders = gd.getNextBoolean();
Prefs.noRowNumbers = !gd.getNextBoolean();
Prefs.dontSaveHeaders = !gd.getNextBoolean();
Prefs.dontSaveRowNumbers = !gd.getNextBoolean();
return;
}
// Miscellaneous Options
void miscOptions() {
String key = IJ.isMacintosh() ? "command" : "control";
GenericDialog gd = new GenericDialog("Miscellaneous Options", IJ.getInstance());
gd.addStringField("Divide by zero value:", "" + FloatBlitter.divideByZeroValue, 10);
gd.addCheckbox("Use pointer cursor", Prefs.usePointerCursor);
gd.addCheckbox("Hide \"Process Stack?\" dialog", IJ.hideProcessStackDialog);
gd.addCheckbox("Require " + key + " key for shortcuts", Prefs.requireControlKey);
gd.addCheckbox("Move isolated plugins to Misc. menu", Prefs.moveToMisc);
if (!IJ.isMacOSX()) gd.addCheckbox("Run single instance listener", Prefs.runSocketListener);
gd.addCheckbox("Enhanced line tool", Prefs.enhancedLineTool);
gd.addCheckbox("Reverse CZT order of \">\" and \"<\"", Prefs.reverseNextPreviousOrder);
gd.addCheckbox("Debug mode", IJ.debugMode);
gd.addHelp(IJ.URL + "/docs/menus/edit.html#misc");
gd.showDialog();
if (gd.wasCanceled()) return;
String divValue = gd.getNextString();
if (divValue.equalsIgnoreCase("infinity") || divValue.equalsIgnoreCase("infinite"))
FloatBlitter.divideByZeroValue = Float.POSITIVE_INFINITY;
else if (divValue.equalsIgnoreCase("NaN")) FloatBlitter.divideByZeroValue = Float.NaN;
else if (divValue.equalsIgnoreCase("max")) FloatBlitter.divideByZeroValue = Float.MAX_VALUE;
else {
Float f;
try {
f = new Float(divValue);
} catch (NumberFormatException e) {
f = null;
}
if (f != null) FloatBlitter.divideByZeroValue = f.floatValue();
}
IJ.register(FloatBlitter.class);
Prefs.usePointerCursor = gd.getNextBoolean();
IJ.hideProcessStackDialog = gd.getNextBoolean();
Prefs.requireControlKey = gd.getNextBoolean();
Prefs.moveToMisc = gd.getNextBoolean();
if (!IJ.isMacOSX()) Prefs.runSocketListener = gd.getNextBoolean();
Prefs.enhancedLineTool = gd.getNextBoolean();
Prefs.reverseNextPreviousOrder = gd.getNextBoolean();
IJ.setDebugMode(gd.getNextBoolean());
}
// DICOM options
void dicom() {
GenericDialog gd = new GenericDialog("DICOM Options");
gd.addCheckbox("Open as 32-bit float", Prefs.openDicomsAsFloat);
// gd.addCheckbox("Calculate voxel depth", Prefs.calculateDicomVoxelDepth);
gd.addMessage("Orthogonal Views");
gd.setInsets(5, 40, 0);
gd.addCheckbox("Rotate YZ", Prefs.rotateYZ);
gd.setInsets(0, 40, 0);
gd.addCheckbox("Flip XZ", Prefs.flipXZ);
gd.showDialog();
if (gd.wasCanceled()) return;
Prefs.openDicomsAsFloat = gd.getNextBoolean();
// Prefs.calculateDicomVoxelDepth = gd.getNextBoolean();
Prefs.rotateYZ = gd.getNextBoolean();
Prefs.flipXZ = gd.getNextBoolean();
}
private boolean showDialog() {
String[] types = {"RAW", "JPEG", "ZLIB"};
GenericDialog gd = new GenericDialog("Generate Bricks");
gd.addChoice("FileType", types, filetype);
gd.addNumericField("JPEG quality", jpeg_quality, 0);
gd.addNumericField("Max file size (MB)", bdsizelimit, 0);
int[] wlist = WindowManager.getIDList();
if (wlist == null) return false;
String[] titles = new String[wlist.length];
for (int i = 0; i < wlist.length; i++) titles[i] = "";
int tnum = 0;
for (int i = 0; i < wlist.length; i++) {
ImagePlus imp = WindowManager.getImage(wlist[i]);
if (imp != null) {
titles[tnum] = imp.getTitle();
tnum++;
}
}
gd.addChoice("Source image: ", titles, titles[0]);
gd.showDialog();
if (gd.wasCanceled()) return false;
filetype = types[gd.getNextChoiceIndex()];
jpeg_quality = (int) gd.getNextNumber();
if (jpeg_quality > 100) jpeg_quality = 100;
if (jpeg_quality < 0) jpeg_quality = 0;
bdsizelimit = (int) gd.getNextNumber();
int id = gd.getNextChoiceIndex();
lvImgTitle = new ArrayList<String>();
lvImgTitle.add(titles[id]);
Prefs.set("filetype.string", filetype);
Prefs.set("jpeg_quality.int", jpeg_quality);
Prefs.set("bdsizelimit.int", bdsizelimit);
return true;
}
public void run(String arg) {
GenericDialog gd = new GenericDialog("Options");
gd.addCheckbox("Acceptor_First", true);
gd.showDialog();
if (gd.wasCanceled()) return;
boolean a1 = gd.getNextBoolean();
int asp = 0;
int dsp = 1;
int aop = 2;
int dop = 3;
int atp = 4;
int dtp = 5;
if (!a1) {
asp = 1;
dsp = 0;
aop = 3;
dop = 2;
atp = 5;
dtp = 4;
}
ImageWindow iw = WindowManager.getCurrentWindow();
float[][] xvals = (float[][]) jutils.runPW4VoidMethod(iw, "getXValues");
float[][] yvals = (float[][]) jutils.runPW4VoidMethod(iw, "getYValues");
int[] npts = (int[]) jutils.runPW4VoidMethod(iw, "getNpts");
int maxpts = (int) jstatistics.getstatistic("Max", npts, null);
int nsets = npts.length / 6;
float[][] rtxvals = new float[nsets][maxpts];
float[][] rtavals = new float[nsets][maxpts];
float[][] rtdvals = new float[nsets][maxpts];
int[] rtnpts = new int[nsets];
int fretlength = 10;
float[][] stfretvals = new float[nsets][fretlength];
float[][] offfretvals = new float[nsets][fretlength];
TextWindow tw = jutils.selectTable("DNA Damage FRET");
if (tw == null)
tw =
new TextWindow(
"DNA Damage FRET",
"title\tacceptor\tdonor\testripe\teoff\tmaxart\tmaxdrt",
"",
400,
200);
// note that st stands for stripe and off is for areas off the damage stripe
for (int i = 0; i < npts.length / 6; i++) {
int len = npts[i * 6];
int damageindex = findbleach(yvals[i * 6 + dsp], len);
int fretindex = findbleach(yvals[i * 6 + atp], len);
IJ.log("set " + i + " damage pos = " + damageindex + " , fret pos = " + fretindex);
int predamagestart = damageindex - 4;
int prefretstart = fretindex - 5;
float staccpredam = getavg(yvals[i * 6 + asp], len, predamagestart, damageindex - 1);
float stdonpredam = getavg(yvals[i * 6 + dsp], len, predamagestart, damageindex - 1);
float nucaccpredam = getavg(yvals[i * 6 + atp], len, predamagestart, damageindex - 1);
float nucdonpredam = getavg(yvals[i * 6 + dtp], len, predamagestart, damageindex - 1);
float stdonprefret = getavg(yvals[i * 6 + dsp], len, prefretstart, fretindex - 2);
float stdonafret = getavg(yvals[i * 6 + dsp], len, fretindex, fretindex + 3);
float offdonprefret = getavg(yvals[i * 6 + dop], len, prefretstart, fretindex - 2);
float offdonafret = getavg(yvals[i * 6 + dop], len, fretindex, fretindex + 3);
float estripe = 1.0f - stdonprefret / stdonafret;
float eoff = 1.0f - offdonprefret / offdonafret;
rtnpts[i] = len;
for (int j = 0; j < len; j++) {
rtxvals[i][j] = j - damageindex - 1;
rtavals[i][j] =
(yvals[i * 6 + asp][j] / staccpredam) / (yvals[i * 6 + atp][j] / nucaccpredam);
rtdvals[i][j] =
(yvals[i * 6 + dsp][j] / stdonpredam) / (yvals[i * 6 + dtp][j] / nucdonpredam);
}
float[] smart = (float[]) algutils.get_subarray(rtavals[i], 0, fretindex);
float[] smdrt = (float[]) algutils.get_subarray(rtdvals[i], 0, fretindex);
jsmooth.blur1D(smart, 2.0f);
jsmooth.blur1D(smdrt, 2.0f);
float maxart = 0.0f;
float maxdrt = 0.0f;
for (int j = 0; j < fretindex - 2; j++) {
if (smart[j] > maxart) maxart = smart[j];
if (smdrt[j] > maxdrt) maxdrt = smdrt[j];
}
stfretvals[i] = getregion(yvals[i * 6 + dsp], len, prefretstart, fretlength);
offfretvals[i] = getregion(yvals[i * 6 + dop], len, prefretstart, fretlength);
tw.append(
iw.getTitle()
+ "-"
+ (i + 1)
+ "\t"
+ staccpredam
+ "\t"
+ stdonpredam
+ "\t"
+ estripe
+ "\t"
+ eoff
+ "\t"
+ maxart
+ "\t"
+ maxdrt);
}
new PlotWindow4("Stripe_FRET_profiles", "time", "intensity", stfretvals, null).draw();
new PlotWindow4("OffStripe_FRET_profiles", "time", "intensity", offfretvals, null).draw();
new PlotWindow4("Acc_Rt_profiles", "time", "intensity", rtxvals, rtavals, rtnpts).draw();
new PlotWindow4("Don_Rt_profiles", "time", "intensity", rtxvals, rtdvals, rtnpts).draw();
}
private void geterrors() {
GenericDialog gd = new GenericDialog("Options");
float conf = 0.67f;
gd.addNumericField("Confidence Limit", (int) (conf * 100.0f), 5, 10, null);
gd.addChoice("Error Parameter", paramsnames, paramsnames[0]);
double spacing = 0.01;
gd.addNumericField("Chi^2 plot spacing (% of value)?", spacing * 100.0, 2, 10, null);
boolean globalerror = false;
gd.addCheckbox("Global Fit Error?", globalerror);
int dataset = 0;
gd.addNumericField("Data Set (for Global Error)", dataset, 0);
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
conf = 0.01f * (float) gd.getNextNumber();
int paramindex = (int) gd.getNextChoiceIndex();
spacing = 0.01 * gd.getNextNumber();
globalerror = gd.getNextBoolean();
dataset = (int) gd.getNextNumber();
if (globalerror) {
support_plane_errors erclass = new support_plane_errors(this, 0.0001, 50, true, 0.1);
int[] erindeces = {paramindex, dataset};
// need to set up all the matrices
int nsel = 0;
int nparams = 11;
for (int i = 0; i < ncurves; i++) {
if (include[i]) {
nsel++;
}
}
double[][] params = new double[nsel][nparams];
String[][] tempformulas = new String[nsel][nparams];
double[][][] constraints = new double[2][nsel][nparams];
int[][] vflmatrix = new int[nsel][nparams];
float[][] tempdata = new float[nsel][xpts * ypts];
float[][] tempweights = new float[nsel][xpts * ypts];
int nfit = 0;
int counter = 0;
for (int i = 0; i < ncurves; i++) {
if (include[i]) {
for (int j = 0; j < nparams; j++) {
params[counter][j] = globalparams[i][j];
tempformulas[counter][j] = globalformulas[i][j];
constraints[0][counter][j] = globalconstraints[0][i][j];
constraints[1][counter][j] = globalconstraints[1][i][j];
vflmatrix[counter][j] = globalvflmatrix[i][j];
if (vflmatrix[counter][j] == 0 || (j == 0 && vflmatrix[counter][j] == 2)) {
nfit++;
}
}
for (int j = 0; j < xpts; j++) {
for (int k = 0; k < ypts; k++) {
tempdata[counter][j + k * xpts] = (float) ((double) pch[i][j][k] / (double) nmeas[i]);
tempweights[counter][j + k * xpts] = weights[i][j][k];
}
}
counter++;
}
}
int dofnum = xpts * ypts * nsel - (nfit - 1) - 1;
int dofden = xpts * ypts * nsel - nfit - 1;
// double flim=FLimit(dofnum,dofden,(double)conf);
double flim = (new jdist()).FLimit(dofnum, dofden, (double) conf);
IJ.log("FLimit = " + (float) flim);
if (flim == Double.NaN && flim < 1.0) {
IJ.showMessage("Invalid Limiting F Value");
return;
}
double truespacing = Math.abs(params[erindeces[1]][erindeces[0]] * spacing);
double[][] c2plot =
erclass.geterrorsglobal(
params,
vflmatrix,
tempformulas,
paramsnames,
constraints,
tempdata,
tempweights,
flim,
truespacing,
erindeces);
IJ.log("upper limit = " + c2plot[1][0] + " lower limit = " + c2plot[0][0]);
int templength = c2plot[0].length;
float[][] c2plotf = new float[2][templength - 1];
for (int i = 0; i < (templength - 1); i++) {
c2plotf[0][i] = (float) c2plot[0][i + 1];
c2plotf[1][i] = (float) c2plot[1][i + 1];
}
new PlotWindow4(
"c2 plot",
paramsnames[paramindex] + "[" + dataset + "]",
"Chi^2",
c2plotf[0],
c2plotf[1])
.draw();
} else {
support_plane_errors erclass = new support_plane_errors(this, 0.0001, 50, false, 0.1);
int errindex = paramindex;
float[] tempdata = new float[xpts * ypts];
float[] tempweights = new float[xpts * ypts];
for (int i = 0; i < xpts; i++) {
for (int j = 0; j < ypts; j++) {
tempdata[i + j * xpts] = (float) ((double) avg[i][j] / (double) nmeas[ncurves]);
tempweights[i + j * xpts] = avgweights[i][j];
}
}
int nfit = 0;
for (int i = 0; i < 7; i++) {
if (avgfixes[i] == 0) {
nfit++;
}
}
int dofnum = xpts * ypts - (nfit - 1) - 1;
int dofden = xpts * ypts - nfit - 1;
double flim = (new jdist()).FLimit(dofnum, dofden, (double) conf);
IJ.log("FLimit = " + (float) flim);
if (flim == Double.NaN && flim < 1.0) {
IJ.showMessage("Invalid Limiting F Value");
return;
}
double truespacing = Math.abs(avgparams[errindex] * spacing);
double[][] c2plot =
erclass.geterrors(
avgparams,
avgfixes,
avgconstraints,
tempdata,
tempweights,
flim,
truespacing,
errindex);
IJ.log("upper limit = " + c2plot[1][0] + " lower limit = " + c2plot[0][0]);
int templength = c2plot[0].length;
float[][] c2plotf = new float[2][templength - 1];
for (int i = 0; i < (templength - 1); i++) {
c2plotf[0][i] = (float) c2plot[0][i + 1];
c2plotf[1][i] = (float) c2plot[1][i + 1];
}
new PlotWindow4("c2 plot", paramsnames[errindex], "Chi^2", c2plotf[0], c2plotf[1]).draw();
}
}
boolean init_functions() {
GenericDialog gd = new GenericDialog("Fitting Options");
gd.addStringField("Extra Definitions", exdef, 50);
gd.addCheckbox("Weight Using Plot Errors", false);
gd.addStringField("Weighting Equation (y is for data)", weightfunction, 50);
gd.addStringField("Fit_Equation", function, 50);
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
exdef = gd.getNextString();
boolean errweights = gd.getNextBoolean();
weightfunction = gd.getNextString();
function = gd.getNextString();
// first initialize the weights
weights = new float[tempdata.length];
if (errweights
|| weightfunction.equals("")
|| weightfunction == null
|| weightfunction == "1.0") {
if (errweights) {
for (int i = 0; i < tempdata.length; i++) weights[i] = 1.0f / (errs[i] * errs[i]);
} else {
for (int i = 0; i < tempdata.length; i++) weights[i] = 1.0f;
}
} else {
for (int i = 0; i < tempdata.length; i++) {
String script =
"y =" + tempdata[i] + "; " + "x =" + tempx[i] + "; " + "retval=" + weightfunction + ";";
Double temp = new Double(0.0);
try {
temp = (Double) engine.eval(script);
} catch (Exception e) {
IJ.log(e.getMessage());
}
if (!(temp.isInfinite() || temp.isNaN())) {
weights[i] = temp.floatValue();
}
}
}
// now compile the function script
try {
String script1 = exdef + "; retval=" + function + ";";
cs = ce.compile(script1);
} catch (Exception e) {
IJ.log(e.toString());
return false;
}
return true;
}
boolean showoptions(double[] params, int[] fixes) {
// GenericDialog gd=new NonBlockingGenericDialog("Options");
GenericDialog gd = new GenericDialog("Options");
gd.addCheckbox("Check Chi Squared", checkc2);
for (int i = 0; i < 10; i++) {
gd.addNumericField("P" + (i + 1), params[i], 5, 10, null);
gd.addCheckbox("Fix?", (fixes[i] == 1));
}
gd.addCheckbox("Get_Errors", false);
gd.addCheckbox("Set_Constraints", false);
gd.addNumericField("Iterations", iterations, 0, 10, null);
gd.addNumericField("chi squared", c2, 5, 10, null);
gd.addDialogListener(this);
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
checkc2 = gd.getNextBoolean();
for (int i = 0; i < 10; i++) {
params[i] = gd.getNextNumber();
if (gd.getNextBoolean()) {
fixes[i] = 1;
} else {
fixes[i] = 0;
}
}
boolean geterrors = gd.getNextBoolean();
boolean setconstraints = gd.getNextBoolean();
for (int i = 0; i < 10; i++) {
if (function.indexOf("P" + (i + 1)) < 0) {
fixes[i] = 1;
}
}
if (geterrors) {
if (!get_errors(params, fixes)) {
return false;
}
}
if (setconstraints) constraints = get_constraints(params);
return true;
}