void hide() {
ImagePlus imp = IJ.getImage();
Overlay overlay = imp.getOverlay();
if (overlay != null) {
overlay2 = overlay;
imp.setOverlay(null);
}
RoiManager rm = RoiManager.getInstance();
if (rm != null) rm.runCommand("show none");
}
public boolean beadCalibration3d() {
imp = IJ.getImage();
if (imp == null) {
IJ.noImage();
return false;
} else if (imp.getStackSize() == 1) {
IJ.error("Stack required");
return false;
} else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
// In order to support 32bit images, pict[] must be changed to float[], and getPixel(x, y);
// requires a Float.intBitsToFloat() conversion
IJ.error("8 or 16 bit greyscale image required");
return false;
}
width = imp.getWidth();
height = imp.getHeight();
nslices = imp.getStackSize();
imtitle = imp.getTitle();
models[0] = "*None*";
models[1] = "line";
models[2] = "2nd degree polynomial";
models[3] = "3rd degree polynomial";
models[4] = "4th degree polynomial";
GenericDialog gd = new GenericDialog("3D PALM calibration");
gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
gd.addNumericField(
"Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
gd.addCheckbox(
"Show divergence of bead positions against model",
prefs.get("QuickPALM.3Dcal_showDivergence", false));
gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
gd.addMessage("\n\nDon't forget to save the table in the end...");
gd.showDialog();
if (gd.wasCanceled()) return false;
fwhm = gd.getNextNumber();
prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
pthrsh = gd.getNextNumber() / 100;
prefs.set("QuickPALM.pthrsh", pthrsh * 100);
cal_z = gd.getNextNumber();
prefs.set("QuickPALM.z-step", cal_z);
window = (int) gd.getNextNumber();
prefs.set("QuickPALM.window", window);
model = gd.getNextChoice();
prefs.set("QuickPALM.model", model);
part_divergence = gd.getNextBoolean();
prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
part_extrainfo = gd.getNextBoolean();
prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
return true;
}
void addSelection() {
ImagePlus imp = IJ.getImage();
String macroOptions = Macro.getOptions();
if (macroOptions != null && IJ.macroRunning() && macroOptions.indexOf("remove") != -1) {
imp.setOverlay(null);
return;
}
Roi roi = imp.getRoi();
if (roi == null && imp.getOverlay() != null) {
GenericDialog gd = new GenericDialog("No Selection");
gd.addMessage("\"Overlay>Add\" requires a selection.");
gd.setInsets(15, 40, 0);
gd.addCheckbox("Remove existing overlay", false);
gd.showDialog();
if (gd.wasCanceled()) return;
if (gd.getNextBoolean()) imp.setOverlay(null);
return;
}
if (roi == null) {
IJ.error("This command requires a selection.");
return;
}
roi = (Roi) roi.clone();
if (roi.getStrokeColor() == null) roi.setStrokeColor(Toolbar.getForegroundColor());
int width = Line.getWidth();
Rectangle bounds = roi.getBounds();
boolean tooWide = width > Math.max(bounds.width, bounds.height) / 3.0;
if (roi.getStroke() == null && width > 1 && !tooWide) roi.setStrokeWidth(Line.getWidth());
Overlay overlay = imp.getOverlay();
if (overlay != null && overlay.size() > 0 && !roi.isDrawingTool()) {
Roi roi2 = overlay.get(overlay.size() - 1);
if (roi.getStroke() == null) roi.setStrokeWidth(roi2.getStrokeWidth());
if (roi.getFillColor() == null) roi.setFillColor(roi2.getFillColor());
}
boolean points = roi instanceof PointRoi && ((PolygonRoi) roi).getNCoordinates() > 1;
if (points) roi.setStrokeColor(Color.red);
if (!IJ.altKeyDown() && !(roi instanceof Arrow)) {
RoiProperties rp = new RoiProperties("Add to Overlay", roi);
if (!rp.showDialog()) return;
}
String name = roi.getName();
boolean newOverlay = name != null && name.equals("new-overlay");
if (overlay == null || newOverlay) overlay = new Overlay();
overlay.add(roi);
imp.setOverlay(overlay);
overlay2 = overlay;
if (points || (roi instanceof ImageRoi) || (roi instanceof Arrow)) imp.killRoi();
Undo.setup(Undo.OVERLAY_ADDITION, imp);
}
public void run(String arg) {
ImagePlus imp = IJ.getImage();
int size = imp.getStackSize();
if (size == 1) {
IJ.error("Z Project", "This command requires a stack");
return;
}
if (imp.isHyperStack()) {
new ZProjector().run("");
return;
}
if (!showDialog(imp)) return;
ImagePlus imp2 = groupZProject(imp, method, groupSize);
if (imp != null) imp2.show();
}
public void run(String arg) {
imp = IJ.getImage();
Roi roi = imp.getRoi();
if (roi != null && !roi.isArea()) imp.killRoi(); // ignore any line selection
ImageProcessor ip = imp.getProcessor();
if (!showDialog(ip)) return;
if (ip.getWidth() > 1 && ip.getHeight() > 1) ip.setInterpolate(interpolate);
else ip.setInterpolate(false);
ip.setBackgroundValue(bgValue);
imp.startTiming();
try {
if (newWindow && imp.getStackSize() > 1 && processStack) createNewStack(imp, ip);
else scale(ip);
} catch (OutOfMemoryError o) {
IJ.outOfMemory("Scale");
}
IJ.showProgress(1.0);
}
void fromRoiManager() {
ImagePlus imp = IJ.getImage();
RoiManager rm = RoiManager.getInstance();
if (rm == null) {
IJ.error("ROI Manager is not open");
return;
}
Roi[] rois = rm.getRoisAsArray();
if (rois.length == 0) {
IJ.error("ROI Manager is empty");
return;
}
Overlay overlay = new Overlay();
for (int i = 0; i < rois.length; i++) overlay.add((Roi) rois[i].clone());
imp.setOverlay(overlay);
ImageCanvas ic = imp.getCanvas();
if (ic != null) ic.setShowAllROIs(false);
rm.setEditMode(imp, false);
imp.killRoi();
}
void toRoiManager() {
ImagePlus imp = IJ.getImage();
Overlay overlay = imp.getOverlay();
if (overlay == null) {
IJ.error("Overlay required");
return;
}
RoiManager rm = RoiManager.getInstance();
if (rm == null) {
if (Macro.getOptions() != null && Interpreter.isBatchMode())
rm = Interpreter.getBatchModeRoiManager();
if (rm == null) {
Frame frame = WindowManager.getFrame("ROI Manager");
if (frame == null) IJ.run("ROI Manager...");
frame = WindowManager.getFrame("ROI Manager");
if (frame == null || !(frame instanceof RoiManager)) return;
rm = (RoiManager) frame;
}
}
rm.runCommand("reset");
for (int i = 0; i < overlay.size(); i++) rm.add(imp, overlay.get(i), i);
rm.setEditMode(imp, true);
if (rm.getCount() == overlay.size()) imp.setOverlay(null);
}
public void run(String arg) {
imp = IJ.getImage();
if (imp == null) return;
stackSize = imp.getStackSize();
Roi roi = imp.getRoi();
Calibration cal = imp.getCalibration();
if (roi != null && roi.getBounds().equals(prevRoi) && cal.pixelWidth == prevPixelWidth)
roi = null;
if (roi != null) {
boolean rectOrOval =
roi != null && (roi.getType() == Roi.RECTANGLE || roi.getType() == Roi.OVAL);
oval = rectOrOval && (roi.getType() == Roi.OVAL); // Handle existing oval ROI
Rectangle r = roi.getBounds();
width = r.width;
height = r.height;
xRoi = r.x;
yRoi = r.y;
if (scaledUnits && cal.scaled()) {
xRoi = xRoi * cal.pixelWidth;
yRoi = yRoi * cal.pixelHeight;
width = width * cal.pixelWidth;
height = height * cal.pixelHeight;
}
if (centered) { // Make xRoi and yRoi consistent when centered mode is active
xRoi += width / 2.0;
yRoi += height / 2.0;
}
} else if (!validDialogValues()) {
width = imp.getWidth() / 2;
height = imp.getHeight() / 2;
xRoi = width / 2;
yRoi = height / 2;
}
iSlice = imp.getCurrentSlice();
showDialog();
}
public void run(String arg) {
imp = IJ.getImage();
showDialog();
}
void addImage() {
ImagePlus imp = IJ.getImage();
int[] wList = WindowManager.getIDList();
if (wList == null || wList.length < 2) {
IJ.error("Add Image...", "The command requires at least two open images.");
return;
}
String[] titles = new String[wList.length];
for (int i = 0; i < wList.length; i++) {
ImagePlus imp2 = WindowManager.getImage(wList[i]);
titles[i] = imp2 != null ? imp2.getTitle() : "";
}
int x = 0, y = 0;
Roi roi = imp.getRoi();
if (roi != null && roi.isArea()) {
Rectangle r = roi.getBounds();
x = r.x;
y = r.y;
}
int index = 0;
if (wList.length == 2) {
ImagePlus i1 = WindowManager.getImage(wList[0]);
ImagePlus i2 = WindowManager.getImage(wList[1]);
if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) index = 1;
} else if (imp.getID() == wList[0]) index = 1;
GenericDialog gd = new GenericDialog("Add Image...");
gd.addChoice("Image to add:", titles, titles[index]);
gd.addNumericField("X location:", x, 0);
gd.addNumericField("Y location:", y, 0);
gd.addNumericField("Opacity (0-100%):", 100, 0);
gd.addCheckbox("Create image selection", createImageRoi);
gd.showDialog();
if (gd.wasCanceled()) return;
index = gd.getNextChoiceIndex();
x = (int) gd.getNextNumber();
y = (int) gd.getNextNumber();
double opacity = gd.getNextNumber() / 100.0;
createImageRoi = gd.getNextBoolean();
ImagePlus overlay = WindowManager.getImage(wList[index]);
if (wList.length == 2) {
ImagePlus i1 = WindowManager.getImage(wList[0]);
ImagePlus i2 = WindowManager.getImage(wList[1]);
if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) {
imp = i1;
overlay = i2;
}
}
if (overlay == imp) {
IJ.error(
"Add Image...", "Image to be added cannot be the same as\n\"" + imp.getTitle() + "\".");
return;
}
if (overlay.getWidth() > imp.getWidth() && overlay.getHeight() > imp.getHeight()) {
IJ.error(
"Add Image...", "Image to be added cannnot be larger than\n\"" + imp.getTitle() + "\".");
return;
}
if (createImageRoi && x == 0 && y == 0) {
x = imp.getWidth() / 2 - overlay.getWidth() / 2;
y = imp.getHeight() / 2 - overlay.getHeight() / 2;
}
roi = new ImageRoi(x, y, overlay.getProcessor());
roi.setName(overlay.getShortTitle());
if (opacity != 1.0) ((ImageRoi) roi).setOpacity(opacity);
if (createImageRoi) imp.setRoi(roi);
else {
Overlay overlayList = imp.getOverlay();
if (overlayList == null) overlayList = new Overlay();
overlayList.add(roi);
imp.setOverlay(overlayList);
overlay2 = overlayList;
Undo.setup(Undo.OVERLAY_ADDITION, imp);
}
}
void flatten() {
ImagePlus imp = IJ.getImage();
ImagePlus imp2 = imp.flatten();
imp2.setTitle(WindowManager.getUniqueName(imp.getTitle()));
imp2.show();
}
void show() {
ImagePlus imp = IJ.getImage();
if (overlay2 != null) imp.setOverlay(overlay2);
RoiManager rm = RoiManager.getInstance();
if (rm != null) rm.runCommand("show all");
}
public void run(String arg) {
ImageCheck ic = new ImageCheck();
if (!ImageCheck.checkEnvironment()) return;
ImagePlus imp = IJ.getImage();
if (!ic.isBinary(imp)) {
IJ.error("8-bit binary (black and white only) image required.");
return;
}
if (!ic.isVoxelIsotropic(imp, 1E-3)) {
if (IJ.showMessageWithCancel(
"Anisotropic voxels",
"This image contains anisotropic voxels, which will\n"
+ "result in incorrect thickness calculation.\n\n"
+ "Consider rescaling your data so that voxels are isotropic\n"
+ "(Image > Scale...).\n\n"
+ "Continue anyway?")) {
} else return;
}
GenericDialog gd = new GenericDialog("Options");
gd.addCheckbox("Thickness", true);
gd.addCheckbox("Spacing", false);
gd.addCheckbox("Graphic Result", true);
gd.addCheckbox("Use_ROI_Manager", false);
gd.addCheckbox("Mask thickness map", true);
gd.addHelp("http://bonej.org/thickness");
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
boolean doThickness = gd.getNextBoolean();
boolean doSpacing = gd.getNextBoolean();
boolean doGraphic = gd.getNextBoolean();
boolean doRoi = gd.getNextBoolean();
boolean doMask = gd.getNextBoolean();
long startTime = System.currentTimeMillis();
String title = stripExtension(imp.getTitle());
RoiManager roiMan = RoiManager.getInstance();
// calculate trabecular thickness (Tb.Th)
if (doThickness) {
boolean inverse = false;
ImagePlus impLTC = new ImagePlus();
if (doRoi && roiMan != null) {
ImageStack stack = RoiMan.cropStack(roiMan, imp.getStack(), true, 0, 1);
ImagePlus crop = new ImagePlus(imp.getTitle(), stack);
crop.setCalibration(imp.getCalibration());
impLTC = getLocalThickness(crop, inverse, doMask);
} else impLTC = getLocalThickness(imp, inverse, doMask);
impLTC.setTitle(title + "_Tb.Th");
impLTC.setCalibration(imp.getCalibration());
double[] stats = StackStats.meanStdDev(impLTC);
insertResults(imp, stats, inverse);
if (doGraphic && !Interpreter.isBatchMode()) {
impLTC.show();
impLTC.setSlice(1);
impLTC.getProcessor().setMinAndMax(0, stats[2]);
IJ.run("Fire");
}
}
if (doSpacing) {
boolean inverse = true;
ImagePlus impLTCi = new ImagePlus();
if (doRoi && roiMan != null) {
ImageStack stack = RoiMan.cropStack(roiMan, imp.getStack(), true, 255, 1);
ImagePlus crop = new ImagePlus(imp.getTitle(), stack);
crop.setCalibration(imp.getCalibration());
impLTCi = getLocalThickness(crop, inverse, doMask);
} else impLTCi = getLocalThickness(imp, inverse, doMask);
// check marrow cavity size (i.e. trabcular separation, Tb.Sp)
impLTCi.setTitle(title + "_Tb.Sp");
impLTCi.setCalibration(imp.getCalibration());
double[] stats = StackStats.meanStdDev(impLTCi);
insertResults(imp, stats, inverse);
if (doGraphic && !Interpreter.isBatchMode()) {
impLTCi.show();
impLTCi.setSlice(1);
impLTCi.getProcessor().setMinAndMax(0, stats[2]);
IJ.run("Fire");
}
}
IJ.showProgress(1.0);
IJ.showStatus("Done");
double duration = ((double) System.currentTimeMillis() - (double) startTime) / (double) 1000;
IJ.log("Duration = " + IJ.d2s(duration, 3) + " s");
UsageReporter.reportEvent(this).send();
return;
}
/** Ask for parameters and then execute. */
public void run(String arg) {
// 1 - Obtain the currently active image:
ImagePlus imp = IJ.getImage();
if (null == imp) {
IJ.showMessage("There must be at least one image open");
return;
}
if (imp.getBitDepth() != 8) {
IJ.showMessage("Error", "Only 8-bit images are supported");
return;
}
// 2 - Ask for parameters:
GenericDialog gd = new GenericDialog("Auto Local Threshold");
String[] methods = {
"Try all",
"Bernsen",
"Contrast",
"Mean",
"Median",
"MidGrey",
"Niblack",
"Otsu",
"Phansalkar",
"Sauvola"
};
gd.addMessage("Auto Local Threshold v1.5");
gd.addChoice("Method", methods, methods[0]);
gd.addNumericField("Radius", 15, 0);
gd.addMessage("Special paramters (if different from default)");
gd.addNumericField("Parameter_1", 0, 0);
gd.addNumericField("Parameter_2", 0, 0);
gd.addCheckbox("White objects on black background", true);
if (imp.getStackSize() > 1) {
gd.addCheckbox("Stack", false);
}
gd.addMessage("Thresholded result is always shown in white [255].");
gd.showDialog();
if (gd.wasCanceled()) return;
// 3 - Retrieve parameters from the dialog
String myMethod = gd.getNextChoice();
int radius = (int) gd.getNextNumber();
double par1 = (double) gd.getNextNumber();
double par2 = (double) gd.getNextNumber();
boolean doIwhite = gd.getNextBoolean();
boolean doIstack = false;
int stackSize = imp.getStackSize();
if (stackSize > 1) doIstack = gd.getNextBoolean();
// 4 - Execute!
// long start = System.currentTimeMillis();
if (myMethod.equals("Try all")) {
ImageProcessor ip = imp.getProcessor();
int xe = ip.getWidth();
int ye = ip.getHeight();
int ml = methods.length;
ImagePlus imp2, imp3;
ImageStack tstack = null, stackNew;
if (stackSize > 1 && doIstack) {
boolean doItAnyway = true;
if (stackSize > 25) {
YesNoCancelDialog d =
new YesNoCancelDialog(
IJ.getInstance(),
"Auto Local Threshold",
"You might run out of memory.\n \nDisplay "
+ stackSize
+ " slices?\n \n \'No\' will process without display and\noutput results to the log window.");
if (!d.yesPressed()) {
// doIlog=true; //will show in the log window
doItAnyway = false;
}
if (d.cancelPressed()) return;
}
for (int j = 1; j <= stackSize; j++) {
imp.setSlice(j);
ip = imp.getProcessor();
tstack = new ImageStack(xe, ye);
for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
imp2 = new ImagePlus("Auto Threshold", tstack);
imp2.updateAndDraw();
for (int k = 1; k < ml; k++) {
imp2.setSlice(k);
Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
}
// if (doItAnyway){
CanvasResizer cr = new CanvasResizer();
stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
imp3 = new ImagePlus("Auto Threshold", stackNew);
imp3.updateAndDraw();
MontageMaker mm = new MontageMaker();
mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); // 3 columns and 3 rows
}
imp.setSlice(1);
// if (doItAnyway)
IJ.run("Images to Stack", "method=[Copy (center)] title=Montage");
return;
} else { // single image try all
tstack = new ImageStack(xe, ye);
for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
imp2 = new ImagePlus("Auto Threshold", tstack);
imp2.updateAndDraw();
for (int k = 1; k < ml; k++) {
imp2.setSlice(k);
// IJ.log("analyzing slice with "+methods[k]);
Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
}
// imp2.setSlice(1);
CanvasResizer cr = new CanvasResizer();
stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
imp3 = new ImagePlus("Auto Threshold", stackNew);
imp3.updateAndDraw();
MontageMaker mm = new MontageMaker();
mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true);
return;
}
} else { // selected a method
if (stackSize > 1 && doIstack) { // whole stack
// if (doIstackHistogram) {// one global histogram
// Object[] result = exec(imp, myMethod, noWhite, noBlack, doIwhite, doIset, doIlog,
// doIstackHistogram );
// }
// else{ // slice by slice
for (int k = 1; k <= stackSize; k++) {
imp.setSlice(k);
Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
}
// }
imp.setSlice(1);
} else { // just one slice
Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
}
// 5 - If all went well, show the image:
// not needed here as the source image is binarised
}
}
public void run(String arg) {
imp = IJ.getImage();
int stackSize = imp.getStackSize();
if (imp == null) {
IJ.noImage();
return;
}
// Make sure input image is a stack.
if (stackSize == 1) {
IJ.error("Z Project", "Stack required");
return;
}
// Check for inverting LUT.
if (imp.getProcessor().isInvertedLut()) {
if (!IJ.showMessageWithCancel("ZProjection", lutMessage)) return;
}
// Set default bounds.
int channels = imp.getNChannels();
int frames = imp.getNFrames();
int slices = imp.getNSlices();
isHyperstack =
imp.isHyperStack()
|| (ij.macro.Interpreter.isBatchMode()
&& ((frames > 1 && frames < stackSize) || (slices > 1 && slices < stackSize)));
boolean simpleComposite = channels == stackSize;
if (simpleComposite) isHyperstack = false;
startSlice = 1;
if (isHyperstack) {
int nSlices = imp.getNSlices();
if (nSlices > 1) stopSlice = nSlices;
else stopSlice = imp.getNFrames();
} else stopSlice = stackSize;
// Build control dialog
GenericDialog gd = buildControlDialog(startSlice, stopSlice);
gd.showDialog();
if (gd.wasCanceled()) return;
if (!imp.lock()) return; // exit if in use
long tstart = System.currentTimeMillis();
setStartSlice((int) gd.getNextNumber());
setStopSlice((int) gd.getNextNumber());
method = gd.getNextChoiceIndex();
Prefs.set(METHOD_KEY, method);
if (isHyperstack) {
allTimeFrames = imp.getNFrames() > 1 && imp.getNSlices() > 1 ? gd.getNextBoolean() : false;
doHyperStackProjection(allTimeFrames);
} else if (imp.getType() == ImagePlus.COLOR_RGB) doRGBProjection(true);
else doProjection(true);
if (arg.equals("") && projImage != null) {
long tstop = System.currentTimeMillis();
projImage.setCalibration(imp.getCalibration());
if (simpleComposite) IJ.run(projImage, "Grays", "");
projImage.show("ZProjector: " + IJ.d2s((tstop - tstart) / 1000.0, 2) + " seconds");
}
imp.unlock();
IJ.register(ZProjector.class);
return;
}