/** Perform the algorithm here */
@Override
public boolean run(JEXEntry entry, HashMap<String, JEXData> inputs) {
try {
/*
* inputNames[2] = new TypeName(IMAGE, "Outer Boundary Image"); inputNames[3] = new TypeName(IMAGE, "Inner Boundary Image"); inputNames[4] = new TypeName(IMAGE, "Image To Quantify");
*/
// Collect the inputs
JEXData maximaData = getInputAs(inputs, "Maxima", ROI);
if (maximaData == null) {
return false;
}
JEXData segData = getInputAs(inputs, "Segmented Image", IMAGE);
if (segData == null) {
return false;
}
JEXData maskData = getInputAs(inputs, "Mask Image", IMAGE);
if (maskData == null) {
return false;
}
JEXData imageData = getInputAs(inputs, "Image To Quantify", IMAGE);
if (imageData == null) {
return false;
}
// Gather parameters
// String op = this.parameters.getValueOfParameter("Measurement");
DimTable imageTable = imageData.getDimTable();
TreeMap<DimensionMap, ROIPlus> maximaMap = RoiReader.readObjectToRoiMap(maximaData);
TreeMap<DimensionMap, String> segMap = ImageReader.readObjectToImagePathTable(segData);
TreeMap<DimensionMap, String> maskMap = ImageReader.readObjectToImagePathTable(maskData);
TreeMap<DimensionMap, String> imageMap = ImageReader.readObjectToImagePathTable(imageData);
TreeMap<DimensionMap, Double> results = new TreeMap<DimensionMap, Double>();
double count = 0;
double total = imageTable.mapCount();
for (DimensionMap map : imageTable.getMapIterator()) {
if (this.isCanceled()) {
return false;
}
this.runStuff(map, maximaMap, segMap, maskMap, imageMap, results, this);
count = count + 1;
JEXStatics.statusBar.setProgressPercentage((int) (100 * count / total));
}
String resultsFile = JEXTableWriter.writeTable(this.outputNames[0].getName(), results);
JEXData output0 = FileWriter.makeFileObject(this.outputNames[0].getName(), null, resultsFile);
// Set the outputs
this.realOutputs.add(output0);
// Return status
return true;
} catch (Exception e) {
e.printStackTrace();
return false;
}
}
@Override
public boolean run(JEXEntry optionalEntry) {
if (imageData == null || !imageData.getTypeName().getType().equals(JEXData.IMAGE)) {
return false;
}
// Run the function
TreeMap<DimensionMap, String> images = ImageReader.readObjectToImagePathTable(imageData);
TreeMap<DimensionMap, String> outputMap = new TreeMap<DimensionMap, String>();
int count = 0;
int total = images.size();
for (DimensionMap dim : images.keySet()) {
if (this.isCanceled()) {
return false;
}
String path = images.get(dim);
// File f = new File(path);
// get the image
ImagePlus im = new ImagePlus(path);
ij.process.ImageProcessor imProc = im.getProcessor();
if (imProc == null) continue;
FloatProcessor imp =
(FloatProcessor) im.getProcessor().convertToFloat(); // should be a float processor
int newWidth = (int) (imp.getWidth() * scale);
imp.setInterpolationMethod(ImageProcessor.BILINEAR);
imp = (FloatProcessor) imp.resize(newWidth);
ImagePlus toSave = FunctionUtility.makeImageToSave(imp, "false", bitDepth);
String finalPath = JEXWriter.saveImage(toSave);
outputMap.put(dim.copy(), finalPath);
Logs.log("Finished processing " + count + " of " + total + ".", 1, this);
count++;
// Status bar
int percentage = (int) (100 * ((double) count / (double) images.size()));
JEXStatics.statusBar.setProgressPercentage(percentage);
}
// Set the outputs
output = ImageWriter.makeImageStackFromPaths("temp", outputMap);
output.setDataObjectInfo("Stack binned using binning function");
// Return status
return true;
}
AdjustImageHelperFunction(
JEXData imset, JEXEntry entry, TypeName[] outputNames, ParameterSet parameters) {
// Pass the variables
this.imset = imset;
this.params = parameters;
this.entry = entry;
this.outputNames = outputNames;
// //// Get params
auto = Boolean.parseBoolean(params.getValueOfParameter("Automatic"));
oldMin = Double.parseDouble(params.getValueOfParameter("Old Min"));
oldMax = Double.parseDouble(params.getValueOfParameter("Old Max"));
newMin = Double.parseDouble(params.getValueOfParameter("New Min"));
newMax = Double.parseDouble(params.getValueOfParameter("New Max"));
gamma = Double.parseDouble(params.getValueOfParameter("Gamma"));
depth = Integer.parseInt(params.getValueOfParameter("Output Bit Depth"));
TreeMap<DimensionMap, JEXDataSingle> map = imset.getDataMap();
int length = map.size();
images = new String[length];
dimensions = new DimensionMap[length];
int i = 0;
TreeMap<DimensionMap, String> pathMap = ImageReader.readObjectToImagePathTable(imset);
for (DimensionMap dim : pathMap.keySet()) {
String path = pathMap.get(dim);
dimensions[i] = dim;
images[i] = path;
i++;
}
// Prepare the graphics
imagepanel = new ImagePanel(this, "Adjust image");
displayImage(index);
wrap = new GraphicalFunctionWrap(this, params);
wrap.addStep(
0,
"Select roi",
new String[] {"Automatic", "Old Min", "Old Max", "New Min", "New Max", "Output Bit Depth"});
wrap.setInCentralPanel(imagepanel);
wrap.setDisplayLoopPanel(true);
}
/** Apply the roi to all other images */
public void finishIT() {
auto = Boolean.parseBoolean(params.getValueOfParameter("Automatic"));
oldMin = Double.parseDouble(params.getValueOfParameter("Old Min"));
oldMax = Double.parseDouble(params.getValueOfParameter("Old Max"));
newMin = Double.parseDouble(params.getValueOfParameter("New Min"));
newMax = Double.parseDouble(params.getValueOfParameter("New Max"));
gamma = Double.parseDouble(params.getValueOfParameter("Gamma"));
depth = Integer.parseInt(params.getValueOfParameter("Output Bit Depth"));
output = new JEXData(JEXData.IMAGE, outputNames[0].getName(), "Adjusted image");
// Run the function
// TreeMap<DimensionMap,JEXDataSingle> map = imset.getDataMap();
TreeMap<DimensionMap, String> pathMap = ImageReader.readObjectToImagePathTable(imset);
int count = 0;
int total = pathMap.size();
JEXStatics.statusBar.setProgressPercentage(0);
TreeMap<DimensionMap, String> imMap = new TreeMap<DimensionMap, String>();
for (DimensionMap dim : pathMap.keySet()) {
// JEXDataSingle ds = map.get(dim);
// String imagePath = ds.get(JEXDataSingle.FOLDERNAME) +
// File.separator + ds.get(JEXDataSingle.FILENAME);
String imagePath = pathMap.get(dim);
// File imageFile = new File(imagePath);
// String imageName = imageFile.getName();
// get the image
im = new ImagePlus(imagePath);
imp = (FloatProcessor) im.getProcessor().convertToFloat(); // should
// be a
// float
// processor
// //// Begin Actual Function
adjustImage();
// //// End Actual Function
// //// Save the results
ImagePlus toSave = FunctionUtility.makeImageToSave(imp, "false", depth);
String imPath = JEXWriter.saveImage(toSave);
// Put into imMap
imMap.put(dim, imPath);
// String localDir = JEXWriter.getEntryFolder(entry);
// String newFileName = FunctionUtility.getNextName(localDir,
// imageName, "A");
// String newImagePath = localDir + File.separator + newFileName;
// FunctionUtility.imSave(imp, "false", depth, newImagePath);
//
// // Put into imMap
// imMap.put(dim, localDir + File.separator + newFileName) ;
// JEXDataSingle outputds = new DefaultJEXDataSingle();
// outputds.put(JEXDataSingle.FOLDERNAME, localDir);
// outputds.put(JEXDataSingle.FILENAME, newFileName);
// output.addData(dim,outputds);
// Increment count
Logs.log("Finished processing " + count + " of " + total + ".", 1, this);
count++;
// Status bar
int percentage = (int) (100 * ((double) count / (double) pathMap.size()));
JEXStatics.statusBar.setProgressPercentage(percentage);
}
output = ImageWriter.makeImageStackFromPaths(outputNames[0].getName(), imMap);
}
/** Perform the algorithm here */
@Override
public boolean run(JEXEntry entry, HashMap<String, JEXData> inputs) {
// Collect the inputs
JEXData data1 = inputs.get("Image to quantify");
JEXData data2 = inputs.get("Mask");
if (data1 == null || !data1.getTypeName().getType().equals(JEXData.IMAGE)) return false;
// Run the function
TreeMap<DimensionMap, String> images = ImageReader.readObjectToImagePathTable(data1);
TreeMap<DimensionMap, String> masks = ImageReader.readObjectToImagePathTable(data2);
// Prepare the outputs
TreeMap<DimensionMap, String> means = new TreeMap<DimensionMap, String>();
TreeMap<DimensionMap, String> areas = new TreeMap<DimensionMap, String>();
TreeMap<DimensionMap, String> intdens = new TreeMap<DimensionMap, String>();
// Do the calculation
for (DimensionMap dim : images.keySet()) {
// prepare the variables
double mean = 0;
double area = 0;
double intden = 0;
// Open the image to quantify
String imPath = images.get(dim);
ImagePlus imp = new ImagePlus(imPath);
// Open the mask
String maskPath = masks.get(dim);
ImagePlus imm = new ImagePlus(maskPath);
// calculate integrated intensity in the image to quantify
ImageStatistics imStat = imp.getStatistics();
intden = imStat.mean * imStat.area;
// Calculate the area of the mask
ImageStatistics maskStat = imm.getStatistics();
area = maskStat.mean * maskStat.area / maskStat.max;
// Calculate the mean intensity in the image to quanfity
mean = intden / area;
// save the variables
means.put(dim, "" + mean);
areas.put(dim, "" + area);
intdens.put(dim, "" + intden);
}
// Make the outputs
JEXData output1 = ValueWriter.makeValueTable(outputNames[0].getName(), means);
JEXData output2 = ValueWriter.makeValueTable(outputNames[1].getName(), areas);
JEXData output3 = ValueWriter.makeValueTable(outputNames[2].getName(), intdens);
// Set the outputs
realOutputs.add(output1);
realOutputs.add(output2);
realOutputs.add(output3);
// Return status
return true;
}
