Пример #1
0
  /*.................................................................................................................*/
  public void processAceFileWithoutContig(
      DNAData data,
      String processedAceFilePath,
      AceFile ace,
      String geneName,
      MesquiteString fullName,
      int it,
      MesquiteString voucherCode) {
    DNAData editedData = ChromaseqUtil.getEditedData(data);
    DNAData originalData = ChromaseqUtil.getOriginalData(data);
    Taxa taxa = data.getTaxa();
    ace.processFailedContig(polyThreshold);
    ace.setNameTranslation(fileNameTranslation);

    ace.renameContigs(fullName.toString(), addFragName, geneName);
    ace.setLowQualityToLowerCase(qualThresholdForLowerCase);
    ace.writeToPropertiesFile(contigPropertiesFileBuffer, fullName.toString());
    if (truncateMixedEnds) {
      ace.trimMixedEnds(mixedEndThreshold, mixedEndWindow, qualThresholdForTrim, addPhrapFailures);
    }
    MesquiteFile.putFileContents(processedAceFilePath, ace.toString(processPolymorphisms), true);
    ace.importSequence(
        taxa,
        editedData,
        it,
        originalData,
        ChromaseqUtil.getQualityData(data),
        ChromaseqUtil.getRegistryData(data),
        singleTaxaBlock,
        processPolymorphisms,
        maxChar,
        "",
        true,
        voucherCode);
  }
Пример #2
0
  /*.................................................................................................................*/
  public void processAceFileWithContig(
      CharacterData data,
      MesquiteModule ownerModule,
      String processedAceFilePath,
      String fragmentDirPath,
      AceFile ace,
      SequenceUploader uploader,
      String geneName,
      MesquiteString fullName,
      String baseName,
      MesquiteString voucherCode,
      int it) {
    DNAData editedData = ChromaseqUtil.getEditedData(data);
    DNAData originalData = ChromaseqUtil.getOriginalData(data);
    Taxa taxa = data.getTaxa();
    ace.setNameTranslation(fileNameTranslation);
    ownerModule.log(ace.contigListForLog() + StringUtil.lineEnding());
    if (processPolymorphisms) ace.processPolys(); // creates an additional CO that has polys in it
    if (renameContigsInAceFiles) ace.renameContigs(fullName.toString(), addFragName, geneName);
    ace.setLowQualityToLowerCase(qualThresholdForLowerCase);
    ace.writeToPropertiesFile(contigPropertiesFileBuffer, fullName.toString());
    if (truncateMixedEnds)
      ace.trimMixedEnds(mixedEndThreshold, mixedEndWindow, qualThresholdForTrim, addPhrapFailures);

    /*		if (uploadResultsToDatabase && StringUtil.notEmpty(databaseURL)) {
    			uploader.uploadAceFileToServer(MesquiteXMLToLUtilities.getTOLPageDatabaseURL(databaseURL), ace, processPolymorphisms, qualThresholdForTrim);
    		}
    */
    System.out.println("\n\nfasta file name: " + baseName + " ace file: " + ace);
    MesquiteFile.putFileContents(
        fragmentDirPath
            + MesquiteFile.fileSeparator
            + ChromaseqUtil.processedFastaFolder
            + MesquiteFile.fileSeparator
            + baseName
            + ".fas",
        ace.toFASTAString(processPolymorphisms, qualThresholdForTrim),
        true);
    MesquiteFile.putFileContents(processedAceFilePath, ace.toString(processPolymorphisms), true);
    ace.importSequence(
        taxa,
        editedData,
        it,
        originalData,
        ChromaseqUtil.getQualityData(data),
        ChromaseqUtil.getRegistryData(data),
        singleTaxaBlock,
        processPolymorphisms,
        maxChar,
        " contig ",
        false,
        voucherCode);
  }