Пример #1
0
 /**
  * Creates a new GenericDialog with the specified title. Uses the current image image window as
  * the parent frame or the ImageJ frame if no image windows are open. Dialog parameters are
  * recorded by ImageJ's command recorder but this requires that the first word of each label be
  * unique.
  */
 public GenericDialog(String title) {
   this(
       title,
       WindowManager.getCurrentImage() != null
           ? (Frame) WindowManager.getCurrentImage().getWindow()
           : IJ.getInstance() != null ? IJ.getInstance() : new Frame());
 }
Пример #2
0
 private static Frame getParentFrame() {
   Frame parent =
       WindowManager.getCurrentImage() != null
           ? (Frame) WindowManager.getCurrentImage().getWindow()
           : IJ.getInstance() != null ? IJ.getInstance() : new Frame();
   if (IJ.isMacOSX() && IJ.isJava18()) {
     ImageJ ij = IJ.getInstance();
     if (ij != null && ij.isActive()) parent = ij;
     else parent = null;
   }
   return parent;
 }
Пример #3
0
 public void run(String arg) {
   ImageJ ij = IJ.getInstance();
   ij.setDropTarget(null);
   new DropTarget(ij, this);
   new DropTarget(Toolbar.getInstance(), this);
   new DropTarget(ij.getStatusBar(), this);
 }
Пример #4
0
 public void dragOver(DropTargetDragEvent e) {
   if (IJ.debugMode) IJ.log("DragOver: " + e.getLocation());
   Point loc = e.getLocation();
   int buttonSize = Toolbar.getButtonSize();
   int width = IJ.getInstance().getSize().width;
   openAsVirtualStack = width - loc.x <= buttonSize;
   if (openAsVirtualStack) IJ.showStatus("<<Open as Virtual Stack>>");
   else IJ.showStatus("<<Drag and Drop>>");
 }
Пример #5
0
 public ImageCanvas(ImagePlus imp) {
   this.imp = imp;
   ij = IJ.getInstance();
   int width = imp.getWidth();
   int height = imp.getHeight();
   imageWidth = width;
   imageHeight = height;
   srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
   setDrawingSize(imageWidth, (int) (imageHeight));
   magnification = 1.0;
   addMouseListener(this);
   addMouseMotionListener(this);
   addKeyListener(ij); // ImageJ handles keyboard shortcuts
   setFocusTraversalKeysEnabled(false);
 }
Пример #6
0
  /** Restores original disk or network version of image. */
  public void revertToSaved(ImagePlus imp) {
    Image img;
    ProgressBar pb = IJ.getInstance().getProgressBar();
    ImageProcessor ip;
    String path = fi.directory + fi.fileName;

    if (fi.fileFormat == fi.GIF_OR_JPG) {
      // restore gif or jpg
      img = Toolkit.getDefaultToolkit().createImage(path);
      imp.setImage(img);
      if (imp.getType() == ImagePlus.COLOR_RGB) Opener.convertGrayJpegTo8Bits(imp);
      return;
    }

    if (fi.fileFormat == fi.DICOM) {
      // restore DICOM
      ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.DICOM", path);
      if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
      return;
    }

    if (fi.fileFormat == fi.BMP) {
      // restore BMP
      ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.BMP_Reader", path);
      if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
      return;
    }

    if (fi.fileFormat == fi.PGM) {
      // restore PGM
      ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.PGM_Reader", path);
      if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
      return;
    }

    if (fi.fileFormat == fi.ZIP_ARCHIVE) {
      // restore ".zip" file
      ImagePlus imp2 = (new Opener()).openZip(path);
      if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
      return;
    }

    // restore PNG or another image opened using ImageIO
    if (fi.fileFormat == fi.IMAGEIO) {
      ImagePlus imp2 = (new Opener()).openUsingImageIO(path);
      if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
      return;
    }

    if (fi.nImages > 1) return;

    ColorModel cm;
    if (fi.url == null || fi.url.equals("")) IJ.showStatus("Loading: " + path);
    else IJ.showStatus("Loading: " + fi.url + fi.fileName);
    Object pixels = readPixels(fi);
    if (pixels == null) return;
    cm = createColorModel(fi);
    switch (fi.fileType) {
      case FileInfo.GRAY8:
      case FileInfo.COLOR8:
      case FileInfo.BITMAP:
        ip = new ByteProcessor(width, height, (byte[]) pixels, cm);
        imp.setProcessor(null, ip);
        break;
      case FileInfo.GRAY16_SIGNED:
      case FileInfo.GRAY16_UNSIGNED:
      case FileInfo.GRAY12_UNSIGNED:
        ip = new ShortProcessor(width, height, (short[]) pixels, cm);
        imp.setProcessor(null, ip);
        break;
      case FileInfo.GRAY32_INT:
      case FileInfo.GRAY32_FLOAT:
        ip = new FloatProcessor(width, height, (float[]) pixels, cm);
        imp.setProcessor(null, ip);
        break;
      case FileInfo.RGB:
      case FileInfo.BGR:
      case FileInfo.ARGB:
      case FileInfo.ABGR:
      case FileInfo.RGB_PLANAR:
        img =
            Toolkit.getDefaultToolkit()
                .createImage(new MemoryImageSource(width, height, (int[]) pixels, 0, width));
        imp.setImage(img);
        break;
    }
  }
Пример #7
0
 public static void main(String args[]) {
   if (System.getProperty("java.version").substring(0, 3).compareTo("1.5") < 0) {
     javax.swing.JOptionPane.showMessageDialog(
         null, "ImageJ " + VERSION + " requires Java 1.5 or later.");
     System.exit(0);
   }
   boolean noGUI = false;
   int mode = STANDALONE;
   arguments = args;
   // System.setProperty("file.encoding", "UTF-8");
   int nArgs = args != null ? args.length : 0;
   boolean commandLine = false;
   for (int i = 0; i < nArgs; i++) {
     String arg = args[i];
     if (arg == null) continue;
     if (args[i].startsWith("-")) {
       if (args[i].startsWith("-batch")) noGUI = true;
       else if (args[i].startsWith("-debug")) IJ.setDebugMode(true);
       else if (args[i].startsWith("-ijpath") && i + 1 < nArgs) {
         if (IJ.debugMode) IJ.log("-ijpath: " + args[i + 1]);
         Prefs.setHomeDir(args[i + 1]);
         commandLine = true;
         args[i + 1] = null;
       } else if (args[i].startsWith("-port")) {
         int delta = (int) Tools.parseDouble(args[i].substring(5, args[i].length()), 0.0);
         commandLine = true;
         if (delta == 0) mode = EMBEDDED;
         else if (delta > 0 && DEFAULT_PORT + delta < 65536) port = DEFAULT_PORT + delta;
       }
     }
   }
   // If existing ImageJ instance, pass arguments to it and quit.
   boolean passArgs = mode == STANDALONE && !noGUI;
   if (IJ.isMacOSX() && !commandLine) passArgs = false;
   if (passArgs && isRunning(args)) return;
   ImageJ ij = IJ.getInstance();
   if (!noGUI && (ij == null || (ij != null && !ij.isShowing()))) {
     ij = new ImageJ(null, mode);
     ij.exitWhenQuitting = true;
   }
   int macros = 0;
   for (int i = 0; i < nArgs; i++) {
     String arg = args[i];
     if (arg == null) continue;
     if (arg.startsWith("-")) {
       if ((arg.startsWith("-macro") || arg.startsWith("-batch")) && i + 1 < nArgs) {
         String arg2 = i + 2 < nArgs ? args[i + 2] : null;
         Prefs.commandLineMacro = true;
         if (noGUI && args[i + 1].endsWith(".js")) Interpreter.batchMode = true;
         IJ.runMacroFile(args[i + 1], arg2);
         break;
       } else if (arg.startsWith("-eval") && i + 1 < nArgs) {
         String rtn = IJ.runMacro(args[i + 1]);
         if (rtn != null) System.out.print(rtn);
         args[i + 1] = null;
       } else if (arg.startsWith("-run") && i + 1 < nArgs) {
         IJ.run(args[i + 1]);
         args[i + 1] = null;
       }
     } else if (macros == 0 && (arg.endsWith(".ijm") || arg.endsWith(".txt"))) {
       IJ.runMacroFile(arg);
       macros++;
     } else if (arg.length() > 0 && arg.indexOf("ij.ImageJ") == -1) {
       File file = new File(arg);
       IJ.open(file.getAbsolutePath());
     }
   }
   if (IJ.debugMode && IJ.getInstance() == null) new JavaProperties().run("");
   if (noGUI) System.exit(0);
 }
Пример #8
0
  String getInfo(ImagePlus imp, ImageProcessor ip) {
    String s = new String("");
    if (IJ.getInstance() != null) s += IJ.getInstance().getInfo() + "\n \n";
    s += "Title: " + imp.getTitle() + "\n";
    Calibration cal = imp.getCalibration();
    int stackSize = imp.getStackSize();
    int channels = imp.getNChannels();
    int slices = imp.getNSlices();
    int frames = imp.getNFrames();
    int digits = imp.getBitDepth() == 32 ? 4 : 0;
    int dp, dp2;
    boolean nonUniformUnits = !cal.getXUnit().equals(cal.getYUnit());
    String xunit = cal.getXUnit();
    String yunit = cal.getYUnit();
    String zunit = cal.getZUnit();
    if (cal.scaled()) {
      String xunits = cal.getUnits();
      String yunits = xunits;
      String zunits = xunits;
      if (nonUniformUnits) {
        xunits = xunit;
        yunits = yunit;
        zunits = zunit;
      }
      double pw = imp.getWidth() * cal.pixelWidth;
      double ph = imp.getHeight() * cal.pixelHeight;
      s += "Width:  " + d2s(pw) + " " + xunits + " (" + imp.getWidth() + ")\n";
      s += "Height:  " + d2s(ph) + " " + yunits + " (" + imp.getHeight() + ")\n";
      if (slices > 1) {
        double pd = slices * cal.pixelDepth;
        s += "Depth:  " + d2s(pd) + " " + zunits + " (" + slices + ")\n";
      }
      s += "Size:  " + ImageWindow.getImageSize(imp) + "\n";
      double xResolution = 1.0 / cal.pixelWidth;
      double yResolution = 1.0 / cal.pixelHeight;
      if (xResolution == yResolution)
        s += "Resolution:  " + d2s(xResolution) + " pixels per " + xunit + "\n";
      else {
        s += "X Resolution:  " + d2s(xResolution) + " pixels per " + xunit + "\n";
        s += "Y Resolution:  " + d2s(yResolution) + " pixels per " + yunit + "\n";
      }
    } else {
      s += "Width:  " + imp.getWidth() + " pixels\n";
      s += "Height:  " + imp.getHeight() + " pixels\n";
      if (stackSize > 1) s += "Depth:  " + slices + " pixels\n";
      s += "Size:  " + ImageWindow.getImageSize(imp) + "\n";
    }
    if (stackSize > 1) {
      String vunit = cal.getUnit() + "^3";
      if (nonUniformUnits) vunit = "(" + xunit + " x " + yunit + " x " + zunit + ")";
      s +=
          "Voxel size: "
              + d2s(cal.pixelWidth)
              + "x"
              + d2s(cal.pixelHeight)
              + "x"
              + d2s(cal.pixelDepth)
              + " "
              + vunit
              + "\n";
    } else {
      String punit = cal.getUnit() + "^2";
      if (nonUniformUnits) punit = "(" + xunit + " x " + yunit + ")";
      dp = Tools.getDecimalPlaces(cal.pixelWidth, cal.pixelHeight);
      s += "Pixel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + " " + punit + "\n";
    }

    s += "ID: " + imp.getID() + "\n";
    int type = imp.getType();
    switch (type) {
      case ImagePlus.GRAY8:
        s += "Bits per pixel: 8 ";
        String lut = "LUT";
        if (imp.getProcessor().isColorLut()) lut = "color " + lut;
        else lut = "grayscale " + lut;
        if (imp.isInvertedLut()) lut = "inverting " + lut;
        s += "(" + lut + ")\n";
        if (imp.getNChannels() > 1) s += displayRanges(imp);
        else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n";
        break;
      case ImagePlus.GRAY16:
      case ImagePlus.GRAY32:
        if (type == ImagePlus.GRAY16) {
          String sign = cal.isSigned16Bit() ? "signed" : "unsigned";
          s += "Bits per pixel: 16 (" + sign + ")\n";
        } else s += "Bits per pixel: 32 (float)\n";
        if (imp.getNChannels() > 1) s += displayRanges(imp);
        else {
          s += "Display range: ";
          double min = ip.getMin();
          double max = ip.getMax();
          if (cal.calibrated()) {
            min = cal.getCValue((int) min);
            max = cal.getCValue((int) max);
          }
          s += d2s(min) + " - " + d2s(max) + "\n";
        }
        break;
      case ImagePlus.COLOR_256:
        s += "Bits per pixel: 8 (color LUT)\n";
        break;
      case ImagePlus.COLOR_RGB:
        s += "Bits per pixel: 32 (RGB)\n";
        break;
    }
    double interval = cal.frameInterval;
    double fps = cal.fps;
    if (stackSize > 1) {
      ImageStack stack = imp.getStack();
      int slice = imp.getCurrentSlice();
      String number = slice + "/" + stackSize;
      String label = stack.getShortSliceLabel(slice);
      if (label != null && label.length() > 0) label = " (" + label + ")";
      else label = "";
      if (interval > 0.0 || fps != 0.0) {
        s += "Frame: " + number + label + "\n";
        if (fps != 0.0) {
          String sRate =
              Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5);
          s += "Frame rate: " + sRate + " fps\n";
        }
        if (interval != 0.0)
          s +=
              "Frame interval: "
                  + ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5))
                  + " "
                  + cal.getTimeUnit()
                  + "\n";
      } else s += "Image: " + number + label + "\n";
      if (imp.isHyperStack()) {
        if (channels > 1) s += "  Channel: " + imp.getChannel() + "/" + channels + "\n";
        if (slices > 1) s += "  Slice: " + imp.getSlice() + "/" + slices + "\n";
        if (frames > 1) s += "  Frame: " + imp.getFrame() + "/" + frames + "\n";
      }
      if (imp.isComposite()) {
        if (!imp.isHyperStack() && channels > 1) s += "  Channels: " + channels + "\n";
        String mode = ((CompositeImage) imp).getModeAsString();
        s += "  Composite mode: \"" + mode + "\"\n";
      }
    }

    if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No threshold\n";
    else {
      double lower = ip.getMinThreshold();
      double upper = ip.getMaxThreshold();
      if (cal.calibrated()) {
        lower = cal.getCValue((int) lower);
        upper = cal.getCValue((int) upper);
      }
      s += "Threshold: " + d2s(lower) + "-" + d2s(upper) + "\n";
    }
    ImageCanvas ic = imp.getCanvas();
    double mag = ic != null ? ic.getMagnification() : 1.0;
    if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n";

    if (cal.calibrated()) {
      s += " \n";
      int curveFit = cal.getFunction();
      s += "Calibration function: ";
      if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n";
      else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n";
      else s += CurveFitter.fList[curveFit] + "\n";
      double[] c = cal.getCoefficients();
      if (c != null) {
        s += "  a: " + IJ.d2s(c[0], 6) + "\n";
        s += "  b: " + IJ.d2s(c[1], 6) + "\n";
        if (c.length >= 3) s += "  c: " + IJ.d2s(c[2], 6) + "\n";
        if (c.length >= 4) s += "  c: " + IJ.d2s(c[3], 6) + "\n";
        if (c.length >= 5) s += "  c: " + IJ.d2s(c[4], 6) + "\n";
      }
      s += "  Unit: \"" + cal.getValueUnit() + "\"\n";
    } else s += "Uncalibrated\n";

    FileInfo fi = imp.getOriginalFileInfo();
    if (fi != null) {
      if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n";
      else if (fi.directory != null && fi.fileName != null)
        s += "Path: " + fi.directory + fi.fileName + "\n";
    }

    ImageWindow win = imp.getWindow();
    if (win != null) {
      Point loc = win.getLocation();
      Dimension screen = IJ.getScreenSize();
      s +=
          "Screen location: "
              + loc.x
              + ","
              + loc.y
              + " ("
              + screen.width
              + "x"
              + screen.height
              + ")\n";
    }

    String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : "";
    String origin = d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin;
    if (!origin.equals("0,0") || cal.getInvertY()) s += "Coordinate origin:  " + origin + "\n";
    if (cal.getInvertY()) s += "Inverted y coordinates\n";

    Overlay overlay = imp.getOverlay();
    if (overlay != null) {
      String hidden = imp.getHideOverlay() ? " (hidden)" : " ";
      int n = overlay.size();
      String elements = n == 1 ? " element" : " elements";
      s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n";
    } else s += "No overlay\n";

    Roi roi = imp.getRoi();
    if (roi == null) {
      if (cal.calibrated()) s += " \n";
      s += "No selection\n";
    } else if (roi instanceof EllipseRoi) {
      s += "\nElliptical selection\n";
      double[] p = ((EllipseRoi) roi).getParams();
      double dx = p[2] - p[0];
      double dy = p[3] - p[1];
      double major = Math.sqrt(dx * dx + dy * dy);
      s += "  Major: " + IJ.d2s(major, 2) + "\n";
      s += "  Minor: " + IJ.d2s(major * p[4], 2) + "\n";
      s += "  X1: " + IJ.d2s(p[0], 2) + "\n";
      s += "  Y1: " + IJ.d2s(p[1], 2) + "\n";
      s += "  X2: " + IJ.d2s(p[2], 2) + "\n";
      s += "  Y2: " + IJ.d2s(p[3], 2) + "\n";
      s += "  Aspect ratio: " + IJ.d2s(p[4], 2) + "\n";
    } else {
      s += " \n";
      s += roi.getTypeAsString() + " Selection";
      String points = null;
      if (roi instanceof PointRoi) {
        int npoints = ((PolygonRoi) roi).getNCoordinates();
        String suffix = npoints > 1 ? "s)" : ")";
        points = " (" + npoints + " point" + suffix;
      }
      String name = roi.getName();
      if (name != null) {
        s += " (\"" + name + "\")";
        if (points != null) s += "\n " + points;
      } else if (points != null) s += points;
      s += "\n";
      Rectangle r = roi.getBounds();
      if (roi instanceof Line) {
        Line line = (Line) roi;
        s += "  X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n";
        s += "  Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n";
        s += "  X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n";
        s += "  Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n";
      } else if (cal.scaled()) {
        s += "  X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n";
        s += "  Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n";
        s += "  Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n";
        s += "  Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n";
      } else {
        s += "  X: " + r.x + "\n";
        s += "  Y: " + yy(r.y, imp) + "\n";
        s += "  Width: " + r.width + "\n";
        s += "  Height: " + r.height + "\n";
      }
    }

    return s;
  }
Пример #9
0
    public BandAdjuster() {

      super("Threshold Colour");
      if (instance != null) {
        instance.toFront();
        return;
      }
      imp = WindowManager.getCurrentImage();
      if (imp == null) {
        IJ.beep();
        IJ.showStatus("No image");
        return;
      }
      IJ.run("Select None");
      thread = new Thread(this, "BandAdjuster");
      WindowManager.addWindow(this);
      instance = this;
      IJ.register(PasteController.class);

      ij = IJ.getInstance();
      Font font = new Font("SansSerif", Font.PLAIN, 10);
      GridBagLayout gridbag = new GridBagLayout();
      GridBagConstraints c = new GridBagConstraints();
      setLayout(gridbag);

      int y = 0;
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      labelh = new Label("Hue", Label.CENTER);
      add(labelh, c);

      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(7, 0, 0, 0);
      labelf = new Label("Filter type", Label.RIGHT);
      add(labelf, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(plot, c);

      // checkboxes
      panelh = new Panel();
      filterTypeH = new CheckboxGroup();
      bandPassH = new Checkbox("Pass");
      bandPassH.setCheckboxGroup(filterTypeH);
      bandPassH.addItemListener(this);
      panelh.add(bandPassH);
      bandStopH = new Checkbox("Stop");
      bandStopH.setCheckboxGroup(filterTypeH);
      bandStopH.addItemListener(this);
      panelh.add(bandStopH);
      bandPassH.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelh, c);

      // minHue slider
      minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);

      add(minSlider, c);
      minSlider.addAdjustmentListener(this);
      minSlider.setUnitIncrement(1);

      // minHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label1 = new Label("       ", Label.LEFT);
      label1.setFont(font);
      add(label1, c);

      // maxHue sliderHue
      maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider, c);
      maxSlider.addAdjustmentListener(this);
      maxSlider.setUnitIncrement(1);

      // maxHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label2 = new Label("       ", Label.LEFT);
      label2.setFont(font);
      add(label2, c);

      // =====
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labels = new Label("Saturation", Label.CENTER);
      add(labels, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(splot, c);

      // checkboxes
      panels = new Panel();
      filterTypeS = new CheckboxGroup();
      bandPassS = new Checkbox("Pass");
      bandPassS.setCheckboxGroup(filterTypeS);
      bandPassS.addItemListener(this);
      panels.add(bandPassS);
      bandStopS = new Checkbox("Stop");
      bandStopS.setCheckboxGroup(filterTypeS);
      bandStopS.addItemListener(this);
      panels.add(bandStopS);
      bandPassS.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panels, c);

      // minSat slider
      minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider2, c);
      minSlider2.addAdjustmentListener(this);
      minSlider2.setUnitIncrement(1);

      // minSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label3 = new Label("       ", Label.LEFT);
      label3.setFont(font);
      add(label3, c);

      // maxSat slider
      maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider2, c);
      maxSlider2.addAdjustmentListener(this);
      maxSlider2.setUnitIncrement(1);

      // maxSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label4 = new Label("       ", Label.LEFT);
      label4.setFont(font);
      add(label4, c);

      // =====
      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labelb = new Label("Brightness", Label.CENTER);
      add(labelb, c);

      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(bplot, c);

      // checkboxes
      panelb = new Panel();
      filterTypeB = new CheckboxGroup();
      bandPassB = new Checkbox("Pass");
      bandPassB.setCheckboxGroup(filterTypeB);
      bandPassB.addItemListener(this);
      panelb.add(bandPassB);
      bandStopB = new Checkbox("Stop");
      bandStopB.setCheckboxGroup(filterTypeB);
      bandStopB.addItemListener(this);
      panelb.add(bandStopB);
      bandPassB.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelb, c);

      // minBri slider
      minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider3, c);
      minSlider3.addAdjustmentListener(this);
      minSlider3.setUnitIncrement(1);

      // minBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label5 = new Label("       ", Label.LEFT);
      label5.setFont(font);
      add(label5, c);

      // maxBri slider
      maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider3, c);
      maxSlider3.addAdjustmentListener(this);
      maxSlider3.setUnitIncrement(1);

      // maxBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label6 = new Label("       ", Label.LEFT);
      label6.setFont(font);
      add(label6, c);

      // =====
      panelt = new Panel();
      threshold = new Checkbox("Threshold");
      threshold.addItemListener(this);
      panelt.add(threshold);

      invert = new Checkbox("Invert");
      invert.addItemListener(this);
      panelt.add(invert);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelt, c);

      // buttons
      panel = new Panel();
      // panel.setLayout(new GridLayout(2, 2, 0, 0));
      originalB = new Button("Original");
      originalB.setEnabled(false);
      originalB.addActionListener(this);
      originalB.addKeyListener(ij);
      panel.add(originalB);

      filteredB = new Button("Filtered");
      filteredB.setEnabled(false);
      filteredB.addActionListener(this);
      filteredB.addKeyListener(ij);
      panel.add(filteredB);

      stackB = new Button("Stack");
      stackB.addActionListener(this);
      stackB.addKeyListener(ij);
      panel.add(stackB);

      helpB = new Button("Help");
      helpB.addActionListener(this);
      helpB.addKeyListener(ij);
      panel.add(helpB);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panel, c);

      panelMode = new Panel();

      sampleB = new Button("Sample");
      sampleB.addActionListener(this);
      sampleB.addKeyListener(ij);
      panelMode.add(sampleB);

      colourMode = new CheckboxGroup();
      hsb = new Checkbox("HSB");
      hsb.setCheckboxGroup(colourMode);
      hsb.addItemListener(this);
      panelMode.add(hsb);
      hsb.setState(true);
      rgb = new Checkbox("RGB");
      rgb.setCheckboxGroup(colourMode);
      rgb.addItemListener(this);
      panelMode.add(rgb);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelMode, c);

      addKeyListener(ij); // ImageJ handles keyboard shortcuts
      pack();
      GUI.center(this);
      setVisible(true);

      ip = setup(imp);
      if (ip == null) {
        imp.unlock();
        IJ.beep();
        IJ.showStatus("RGB image cannot be thresholded");
        return;
      }
      thread.start();
    }