void createMaskFromThreshold(ImagePlus imp) { ImageProcessor ip = imp.getProcessor(); if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) { IJ.error("Create Mask", "Area selection or thresholded image required"); return; } double t1 = ip.getMinThreshold(); double t2 = ip.getMaxThreshold(); IJ.run("Duplicate...", "title=mask"); ImagePlus imp2 = WindowManager.getCurrentImage(); ImageProcessor ip2 = imp2.getProcessor(); ip2.setThreshold(t1, t2, ImageProcessor.NO_LUT_UPDATE); IJ.run("Convert to Mask"); }
void addToRoiManager(ImagePlus imp) { if (IJ.macroRunning() && Interpreter.isBatchModeRoiManager()) IJ.error("run(\"Add to Manager\") may not work in batch mode macros"); Frame frame = WindowManager.getFrame("ROI Manager"); if (frame == null) IJ.run("ROI Manager..."); if (imp == null) return; Roi roi = imp.getRoi(); if (roi == null) return; frame = WindowManager.getFrame("ROI Manager"); if (frame == null || !(frame instanceof RoiManager)) IJ.error("ROI Manager not found"); RoiManager rm = (RoiManager) frame; boolean altDown = IJ.altKeyDown(); IJ.setKeyUp(IJ.ALL_KEYS); if (altDown && !IJ.macroRunning()) IJ.setKeyDown(KeyEvent.VK_SHIFT); if (roi.getState() == Roi.CONSTRUCTING) { // wait (up to 2 sec.) until ROI finished long start = System.currentTimeMillis(); while (true) { IJ.wait(10); if (roi.getState() != Roi.CONSTRUCTING) break; if ((System.currentTimeMillis() - start) > 2000) { IJ.beep(); IJ.error("Add to Manager", "Selection is not complete"); return; } } } rm.runCommand("add"); IJ.setKeyUp(IJ.ALL_KEYS); }
void runMacro(String arg) { Roi roi = imp.getRoi(); if (IJ.macroRunning()) { String options = Macro.getOptions(); if (options != null && (options.indexOf("grid=") != -1 || options.indexOf("interpolat") != -1)) { IJ.run("Rotate... ", options); // run Image>Transform>Rotate return; } } if (roi == null) { noRoi("Rotate>Selection"); return; } roi = (Roi) roi.clone(); if (arg.equals("rotate")) { double d = Tools.parseDouble(angle); if (Double.isNaN(d)) angle = "15"; String value = IJ.runMacroFile("ij.jar:RotateSelection", angle); if (value != null) angle = value; } else if (arg.equals("enlarge")) { String value = IJ.runMacroFile("ij.jar:EnlargeSelection", enlarge); if (value != null) enlarge = value; Roi.previousRoi = roi; } }
private void rotate(ImagePlus imp) { Roi roi = imp.getRoi(); if (IJ.macroRunning()) { String options = Macro.getOptions(); if (options != null && (options.indexOf("grid=") != -1 || options.indexOf("interpolat") != -1)) { IJ.run("Rotate... ", options); // run Image>Transform>Rotate return; } } (new RoiRotator()).run(""); }
void runMacro(String arg, ImagePlus imp) { boolean rotate = arg.equals("rotate"); Roi roi = imp.getRoi(); if (rotate && IJ.macroRunning()) { String options = Macro.getOptions(); if (options != null && (options.indexOf("grid=") != -1 || options.indexOf("interpolat") != -1)) { IJ.run("Rotate... ", options); // run Image>Transform>Rotate return; } } if (roi == null) { noRoi(rotate ? "Rotate" : "Enlarge"); return; } double dangle = Tools.parseDouble(angle); if (Double.isNaN(dangle)) { dangle = 15; angle = "" + dangle; } if (rotate && (roi instanceof ImageRoi)) { dangle = IJ.getNumber("Angle (degrees):", dangle); ((ImageRoi) roi).rotate(dangle); imp.draw(); angle = "" + dangle; return; } Undo.setup(Undo.ROI, imp); roi = (Roi) roi.clone(); if (rotate) { String value = IJ.runMacroFile("ij.jar:RotateSelection", angle); Roi roi2 = imp.getRoi(); transferProperties(roi, roi2); imp.setRoi(roi2); if (value != null) angle = value; } else if (arg.equals("enlarge")) (new RoiEnlarger()).run(""); }
private void makeBand(ImagePlus imp) { Roi roi = imp.getRoi(); if (roi == null) { noRoi("Make Band"); return; } if (!roi.isArea()) { IJ.error("Make Band", "Area selection required"); return; } Calibration cal = imp.getCalibration(); double pixels = bandSize; double size = pixels * cal.pixelWidth; int decimalPlaces = 0; if ((int) size != size) decimalPlaces = 2; GenericDialog gd = new GenericDialog("Make Band"); gd.addNumericField("Band Size:", size, decimalPlaces, 4, cal.getUnits()); gd.showDialog(); if (gd.wasCanceled()) return; size = gd.getNextNumber(); if (Double.isNaN(size)) { IJ.error("Make Band", "invalid number"); return; } int n = (int) Math.round(size / cal.pixelWidth); if (n > 255) { IJ.error("Make Band", "Cannot make bands wider that 255 pixels"); return; } int width = imp.getWidth(); int height = imp.getHeight(); Rectangle r = roi.getBounds(); ImageProcessor ip = roi.getMask(); if (ip == null) { ip = new ByteProcessor(r.width, r.height); ip.invert(); } ImageProcessor mask = new ByteProcessor(width, height); mask.insert(ip, r.x, r.y); ImagePlus edm = new ImagePlus("mask", mask); boolean saveBlackBackground = Prefs.blackBackground; Prefs.blackBackground = false; IJ.run(edm, "Distance Map", ""); Prefs.blackBackground = saveBlackBackground; ip = edm.getProcessor(); ip.setThreshold(0, n, ImageProcessor.NO_LUT_UPDATE); int xx = -1, yy = -1; for (int x = r.x; x < r.x + r.width; x++) { for (int y = r.y; y < r.y + r.height; y++) { if (ip.getPixel(x, y) < n) { xx = x; yy = y; break; } } if (xx >= 0 || yy >= 0) break; } int count = IJ.doWand(edm, xx, yy, 0, null); if (count <= 0) { IJ.error("Make Band", "Unable to make band"); return; } ShapeRoi roi2 = new ShapeRoi(edm.getRoi()); if (!(roi instanceof ShapeRoi)) roi = new ShapeRoi(roi); ShapeRoi roi1 = (ShapeRoi) roi; roi2 = roi2.not(roi1); imp.setRoi(roi2); bandSize = n; }
public static void main(String args[]) { if (System.getProperty("java.version").substring(0, 3).compareTo("1.5") < 0) { javax.swing.JOptionPane.showMessageDialog( null, "ImageJ " + VERSION + " requires Java 1.5 or later."); System.exit(0); } boolean noGUI = false; int mode = STANDALONE; arguments = args; // System.setProperty("file.encoding", "UTF-8"); int nArgs = args != null ? args.length : 0; boolean commandLine = false; for (int i = 0; i < nArgs; i++) { String arg = args[i]; if (arg == null) continue; if (args[i].startsWith("-")) { if (args[i].startsWith("-batch")) noGUI = true; else if (args[i].startsWith("-debug")) IJ.setDebugMode(true); else if (args[i].startsWith("-ijpath") && i + 1 < nArgs) { if (IJ.debugMode) IJ.log("-ijpath: " + args[i + 1]); Prefs.setHomeDir(args[i + 1]); commandLine = true; args[i + 1] = null; } else if (args[i].startsWith("-port")) { int delta = (int) Tools.parseDouble(args[i].substring(5, args[i].length()), 0.0); commandLine = true; if (delta == 0) mode = EMBEDDED; else if (delta > 0 && DEFAULT_PORT + delta < 65536) port = DEFAULT_PORT + delta; } } } // If existing ImageJ instance, pass arguments to it and quit. boolean passArgs = mode == STANDALONE && !noGUI; if (IJ.isMacOSX() && !commandLine) passArgs = false; if (passArgs && isRunning(args)) return; ImageJ ij = IJ.getInstance(); if (!noGUI && (ij == null || (ij != null && !ij.isShowing()))) { ij = new ImageJ(null, mode); ij.exitWhenQuitting = true; } int macros = 0; for (int i = 0; i < nArgs; i++) { String arg = args[i]; if (arg == null) continue; if (arg.startsWith("-")) { if ((arg.startsWith("-macro") || arg.startsWith("-batch")) && i + 1 < nArgs) { String arg2 = i + 2 < nArgs ? args[i + 2] : null; Prefs.commandLineMacro = true; if (noGUI && args[i + 1].endsWith(".js")) Interpreter.batchMode = true; IJ.runMacroFile(args[i + 1], arg2); break; } else if (arg.startsWith("-eval") && i + 1 < nArgs) { String rtn = IJ.runMacro(args[i + 1]); if (rtn != null) System.out.print(rtn); args[i + 1] = null; } else if (arg.startsWith("-run") && i + 1 < nArgs) { IJ.run(args[i + 1]); args[i + 1] = null; } } else if (macros == 0 && (arg.endsWith(".ijm") || arg.endsWith(".txt"))) { IJ.runMacroFile(arg); macros++; } else if (arg.length() > 0 && arg.indexOf("ij.ImageJ") == -1) { File file = new File(arg); IJ.open(file.getAbsolutePath()); } } if (IJ.debugMode && IJ.getInstance() == null) new JavaProperties().run(""); if (noGUI) System.exit(0); }
public void build_bricks() { ImagePlus imp; ImagePlus orgimp; ImageStack stack; FileInfo finfo; if (lvImgTitle.isEmpty()) return; orgimp = WindowManager.getImage(lvImgTitle.get(0)); imp = orgimp; finfo = imp.getFileInfo(); if (finfo == null) return; int[] dims = imp.getDimensions(); int imageW = dims[0]; int imageH = dims[1]; int nCh = dims[2]; int imageD = dims[3]; int nFrame = dims[4]; int bdepth = imp.getBitDepth(); double xspc = finfo.pixelWidth; double yspc = finfo.pixelHeight; double zspc = finfo.pixelDepth; double z_aspect = Math.max(xspc, yspc) / zspc; int orgW = imageW; int orgH = imageH; int orgD = imageD; double orgxspc = xspc; double orgyspc = yspc; double orgzspc = zspc; lv = lvImgTitle.size(); if (filetype == "JPEG") { for (int l = 0; l < lv; l++) { if (WindowManager.getImage(lvImgTitle.get(l)).getBitDepth() != 8) { IJ.error("A SOURCE IMAGE MUST BE 8BIT GLAYSCALE"); return; } } } // calculate levels /* int baseXY = 256; int baseZ = 256; if (z_aspect < 0.5) baseZ = 128; if (z_aspect > 2.0) baseXY = 128; if (z_aspect >= 0.5 && z_aspect < 1.0) baseZ = (int)(baseZ*z_aspect); if (z_aspect > 1.0 && z_aspect <= 2.0) baseXY = (int)(baseXY/z_aspect); IJ.log("Z_aspect: " + z_aspect); IJ.log("BaseXY: " + baseXY); IJ.log("BaseZ: " + baseZ); */ int baseXY = 256; int baseZ = 128; int dbXY = Math.max(orgW, orgH) / baseXY; if (Math.max(orgW, orgH) % baseXY > 0) dbXY *= 2; int dbZ = orgD / baseZ; if (orgD % baseZ > 0) dbZ *= 2; lv = Math.max(log2(dbXY), log2(dbZ)) + 1; int ww = orgW; int hh = orgH; int dd = orgD; for (int l = 0; l < lv; l++) { int bwnum = ww / baseXY; if (ww % baseXY > 0) bwnum++; int bhnum = hh / baseXY; if (hh % baseXY > 0) bhnum++; int bdnum = dd / baseZ; if (dd % baseZ > 0) bdnum++; if (bwnum % 2 == 0) bwnum++; if (bhnum % 2 == 0) bhnum++; if (bdnum % 2 == 0) bdnum++; int bw = (bwnum <= 1) ? ww : ww / bwnum + 1 + (ww % bwnum > 0 ? 1 : 0); int bh = (bhnum <= 1) ? hh : hh / bhnum + 1 + (hh % bhnum > 0 ? 1 : 0); int bd = (bdnum <= 1) ? dd : dd / bdnum + 1 + (dd % bdnum > 0 ? 1 : 0); bwlist.add(bw); bhlist.add(bh); bdlist.add(bd); IJ.log("LEVEL: " + l); IJ.log(" width: " + ww); IJ.log(" hight: " + hh); IJ.log(" depth: " + dd); IJ.log(" bw: " + bw); IJ.log(" bh: " + bh); IJ.log(" bd: " + bd); int xyl2 = Math.max(ww, hh) / baseXY; if (Math.max(ww, hh) % baseXY > 0) xyl2 *= 2; if (lv - 1 - log2(xyl2) <= l) { ww /= 2; hh /= 2; } IJ.log(" xyl2: " + (lv - 1 - log2(xyl2))); int zl2 = dd / baseZ; if (dd % baseZ > 0) zl2 *= 2; if (lv - 1 - log2(zl2) <= l) dd /= 2; IJ.log(" zl2: " + (lv - 1 - log2(zl2))); if (l < lv - 1) { lvImgTitle.add(lvImgTitle.get(0) + "_level" + (l + 1)); IJ.selectWindow(lvImgTitle.get(0)); IJ.run( "Scale...", "x=- y=- z=- width=" + ww + " height=" + hh + " depth=" + dd + " interpolation=Bicubic average process create title=" + lvImgTitle.get(l + 1)); } } for (int l = 0; l < lv; l++) { IJ.log(lvImgTitle.get(l)); } Document doc = newXMLDocument(); Element root = doc.createElement("BRK"); root.setAttribute("version", "1.0"); root.setAttribute("nLevel", String.valueOf(lv)); root.setAttribute("nChannel", String.valueOf(nCh)); root.setAttribute("nFrame", String.valueOf(nFrame)); doc.appendChild(root); for (int l = 0; l < lv; l++) { IJ.showProgress(0.0); int[] dims2 = imp.getDimensions(); IJ.log( "W: " + String.valueOf(dims2[0]) + " H: " + String.valueOf(dims2[1]) + " C: " + String.valueOf(dims2[2]) + " D: " + String.valueOf(dims2[3]) + " T: " + String.valueOf(dims2[4]) + " b: " + String.valueOf(bdepth)); bw = bwlist.get(l).intValue(); bh = bhlist.get(l).intValue(); bd = bdlist.get(l).intValue(); boolean force_pow2 = false; /* if(IsPowerOf2(bw) && IsPowerOf2(bh) && IsPowerOf2(bd)) force_pow2 = true; if(force_pow2){ //force pow2 if(Pow2(bw) > bw) bw = Pow2(bw)/2; if(Pow2(bh) > bh) bh = Pow2(bh)/2; if(Pow2(bd) > bd) bd = Pow2(bd)/2; } if(bw > imageW) bw = (Pow2(imageW) == imageW) ? imageW : Pow2(imageW)/2; if(bh > imageH) bh = (Pow2(imageH) == imageH) ? imageH : Pow2(imageH)/2; if(bd > imageD) bd = (Pow2(imageD) == imageD) ? imageD : Pow2(imageD)/2; */ if (bw > imageW) bw = imageW; if (bh > imageH) bh = imageH; if (bd > imageD) bd = imageD; if (bw <= 1 || bh <= 1 || bd <= 1) break; if (filetype == "JPEG" && (bw < 8 || bh < 8)) break; Element lvnode = doc.createElement("Level"); lvnode.setAttribute("lv", String.valueOf(l)); lvnode.setAttribute("imageW", String.valueOf(imageW)); lvnode.setAttribute("imageH", String.valueOf(imageH)); lvnode.setAttribute("imageD", String.valueOf(imageD)); lvnode.setAttribute("xspc", String.valueOf(xspc)); lvnode.setAttribute("yspc", String.valueOf(yspc)); lvnode.setAttribute("zspc", String.valueOf(zspc)); lvnode.setAttribute("bitDepth", String.valueOf(bdepth)); root.appendChild(lvnode); Element brksnode = doc.createElement("Bricks"); brksnode.setAttribute("brick_baseW", String.valueOf(bw)); brksnode.setAttribute("brick_baseH", String.valueOf(bh)); brksnode.setAttribute("brick_baseD", String.valueOf(bd)); lvnode.appendChild(brksnode); ArrayList<Brick> bricks = new ArrayList<Brick>(); int mw, mh, md, mw2, mh2, md2; double tx0, ty0, tz0, tx1, ty1, tz1; double bx0, by0, bz0, bx1, by1, bz1; for (int k = 0; k < imageD; k += bd) { if (k > 0) k--; for (int j = 0; j < imageH; j += bh) { if (j > 0) j--; for (int i = 0; i < imageW; i += bw) { if (i > 0) i--; mw = Math.min(bw, imageW - i); mh = Math.min(bh, imageH - j); md = Math.min(bd, imageD - k); if (force_pow2) { mw2 = Pow2(mw); mh2 = Pow2(mh); md2 = Pow2(md); } else { mw2 = mw; mh2 = mh; md2 = md; } if (filetype == "JPEG") { if (mw2 < 8) mw2 = 8; if (mh2 < 8) mh2 = 8; } tx0 = i == 0 ? 0.0d : ((mw2 - mw + 0.5d) / mw2); ty0 = j == 0 ? 0.0d : ((mh2 - mh + 0.5d) / mh2); tz0 = k == 0 ? 0.0d : ((md2 - md + 0.5d) / md2); tx1 = 1.0d - 0.5d / mw2; if (mw < bw) tx1 = 1.0d; if (imageW - i == bw) tx1 = 1.0d; ty1 = 1.0d - 0.5d / mh2; if (mh < bh) ty1 = 1.0d; if (imageH - j == bh) ty1 = 1.0d; tz1 = 1.0d - 0.5d / md2; if (md < bd) tz1 = 1.0d; if (imageD - k == bd) tz1 = 1.0d; bx0 = i == 0 ? 0.0d : (i + 0.5d) / (double) imageW; by0 = j == 0 ? 0.0d : (j + 0.5d) / (double) imageH; bz0 = k == 0 ? 0.0d : (k + 0.5d) / (double) imageD; bx1 = Math.min((i + bw - 0.5d) / (double) imageW, 1.0d); if (imageW - i == bw) bx1 = 1.0d; by1 = Math.min((j + bh - 0.5d) / (double) imageH, 1.0d); if (imageH - j == bh) by1 = 1.0d; bz1 = Math.min((k + bd - 0.5d) / (double) imageD, 1.0d); if (imageD - k == bd) bz1 = 1.0d; int x, y, z; x = i - (mw2 - mw); y = j - (mh2 - mh); z = k - (md2 - md); bricks.add( new Brick( x, y, z, mw2, mh2, md2, 0, 0, tx0, ty0, tz0, tx1, ty1, tz1, bx0, by0, bz0, bx1, by1, bz1)); } } } Element fsnode = doc.createElement("Files"); lvnode.appendChild(fsnode); stack = imp.getStack(); int totalbricknum = nFrame * nCh * bricks.size(); int curbricknum = 0; for (int f = 0; f < nFrame; f++) { for (int ch = 0; ch < nCh; ch++) { int sizelimit = bdsizelimit * 1024 * 1024; int bytecount = 0; int filecount = 0; int pd_bufsize = Math.max(sizelimit, bw * bh * bd * bdepth / 8); byte[] packed_data = new byte[pd_bufsize]; String base_dataname = basename + "_Lv" + String.valueOf(l) + "_Ch" + String.valueOf(ch) + "_Fr" + String.valueOf(f); String current_dataname = base_dataname + "_data" + filecount; Brick b_first = bricks.get(0); if (b_first.z_ != 0) IJ.log("warning"); int st_z = b_first.z_; int ed_z = b_first.z_ + b_first.d_; LinkedList<ImageProcessor> iplist = new LinkedList<ImageProcessor>(); for (int s = st_z; s < ed_z; s++) iplist.add(stack.getProcessor(imp.getStackIndex(ch + 1, s + 1, f + 1))); // ImagePlus test; // ImageStack tsst; // test = NewImage.createByteImage("test", imageW, imageH, imageD, // NewImage.FILL_BLACK); // tsst = test.getStack(); for (int i = 0; i < bricks.size(); i++) { Brick b = bricks.get(i); if (ed_z > b.z_ || st_z < b.z_ + b.d_) { if (b.z_ > st_z) { for (int s = 0; s < b.z_ - st_z; s++) iplist.pollFirst(); st_z = b.z_; } else if (b.z_ < st_z) { IJ.log("warning"); for (int s = st_z - 1; s > b.z_; s--) iplist.addFirst(stack.getProcessor(imp.getStackIndex(ch + 1, s + 1, f + 1))); st_z = b.z_; } if (b.z_ + b.d_ > ed_z) { for (int s = ed_z; s < b.z_ + b.d_; s++) iplist.add(stack.getProcessor(imp.getStackIndex(ch + 1, s + 1, f + 1))); ed_z = b.z_ + b.d_; } else if (b.z_ + b.d_ < ed_z) { IJ.log("warning"); for (int s = 0; s < ed_z - (b.z_ + b.d_); s++) iplist.pollLast(); ed_z = b.z_ + b.d_; } } else { IJ.log("warning"); iplist.clear(); st_z = b.z_; ed_z = b.z_ + b.d_; for (int s = st_z; s < ed_z; s++) iplist.add(stack.getProcessor(imp.getStackIndex(ch + 1, s + 1, f + 1))); } if (iplist.size() != b.d_) { IJ.log("Stack Error"); return; } // int zz = st_z; int bsize = 0; byte[] bdata = new byte[b.w_ * b.h_ * b.d_ * bdepth / 8]; Iterator<ImageProcessor> ipite = iplist.iterator(); while (ipite.hasNext()) { // ImageProcessor tsip = tsst.getProcessor(zz+1); ImageProcessor ip = ipite.next(); ip.setRoi(b.x_, b.y_, b.w_, b.h_); if (bdepth == 8) { byte[] data = (byte[]) ip.crop().getPixels(); System.arraycopy(data, 0, bdata, bsize, data.length); bsize += data.length; } else if (bdepth == 16) { ByteBuffer buffer = ByteBuffer.allocate(b.w_ * b.h_ * bdepth / 8); buffer.order(ByteOrder.LITTLE_ENDIAN); short[] data = (short[]) ip.crop().getPixels(); for (short e : data) buffer.putShort(e); System.arraycopy(buffer.array(), 0, bdata, bsize, buffer.array().length); bsize += buffer.array().length; } else if (bdepth == 32) { ByteBuffer buffer = ByteBuffer.allocate(b.w_ * b.h_ * bdepth / 8); buffer.order(ByteOrder.LITTLE_ENDIAN); float[] data = (float[]) ip.crop().getPixels(); for (float e : data) buffer.putFloat(e); System.arraycopy(buffer.array(), 0, bdata, bsize, buffer.array().length); bsize += buffer.array().length; } } String filename = basename + "_Lv" + String.valueOf(l) + "_Ch" + String.valueOf(ch) + "_Fr" + String.valueOf(f) + "_ID" + String.valueOf(i); int offset = bytecount; int datasize = bdata.length; if (filetype == "RAW") { int dummy = -1; // do nothing } if (filetype == "JPEG" && bdepth == 8) { try { DataBufferByte db = new DataBufferByte(bdata, datasize); Raster raster = Raster.createPackedRaster(db, b.w_, b.h_ * b.d_, 8, null); BufferedImage img = new BufferedImage(b.w_, b.h_ * b.d_, BufferedImage.TYPE_BYTE_GRAY); img.setData(raster); ByteArrayOutputStream baos = new ByteArrayOutputStream(); ImageOutputStream ios = ImageIO.createImageOutputStream(baos); String format = "jpg"; Iterator<javax.imageio.ImageWriter> iter = ImageIO.getImageWritersByFormatName("jpeg"); javax.imageio.ImageWriter writer = iter.next(); ImageWriteParam iwp = writer.getDefaultWriteParam(); iwp.setCompressionMode(ImageWriteParam.MODE_EXPLICIT); iwp.setCompressionQuality((float) jpeg_quality * 0.01f); writer.setOutput(ios); writer.write(null, new IIOImage(img, null, null), iwp); // ImageIO.write(img, format, baos); bdata = baos.toByteArray(); datasize = bdata.length; } catch (IOException e) { e.printStackTrace(); return; } } if (filetype == "ZLIB") { byte[] tmpdata = new byte[b.w_ * b.h_ * b.d_ * bdepth / 8]; Deflater compresser = new Deflater(); compresser.setInput(bdata); compresser.setLevel(Deflater.DEFAULT_COMPRESSION); compresser.setStrategy(Deflater.DEFAULT_STRATEGY); compresser.finish(); datasize = compresser.deflate(tmpdata); bdata = tmpdata; compresser.end(); } if (bytecount + datasize > sizelimit && bytecount > 0) { BufferedOutputStream fis = null; try { File file = new File(directory + current_dataname); fis = new BufferedOutputStream(new FileOutputStream(file)); fis.write(packed_data, 0, bytecount); } catch (IOException e) { e.printStackTrace(); return; } finally { try { if (fis != null) fis.close(); } catch (IOException e) { e.printStackTrace(); return; } } filecount++; current_dataname = base_dataname + "_data" + filecount; bytecount = 0; offset = 0; System.arraycopy(bdata, 0, packed_data, bytecount, datasize); bytecount += datasize; } else { System.arraycopy(bdata, 0, packed_data, bytecount, datasize); bytecount += datasize; } Element filenode = doc.createElement("File"); filenode.setAttribute("filename", current_dataname); filenode.setAttribute("channel", String.valueOf(ch)); filenode.setAttribute("frame", String.valueOf(f)); filenode.setAttribute("brickID", String.valueOf(i)); filenode.setAttribute("offset", String.valueOf(offset)); filenode.setAttribute("datasize", String.valueOf(datasize)); filenode.setAttribute("filetype", String.valueOf(filetype)); fsnode.appendChild(filenode); curbricknum++; IJ.showProgress((double) (curbricknum) / (double) (totalbricknum)); } if (bytecount > 0) { BufferedOutputStream fis = null; try { File file = new File(directory + current_dataname); fis = new BufferedOutputStream(new FileOutputStream(file)); fis.write(packed_data, 0, bytecount); } catch (IOException e) { e.printStackTrace(); return; } finally { try { if (fis != null) fis.close(); } catch (IOException e) { e.printStackTrace(); return; } } } } } for (int i = 0; i < bricks.size(); i++) { Brick b = bricks.get(i); Element bricknode = doc.createElement("Brick"); bricknode.setAttribute("id", String.valueOf(i)); bricknode.setAttribute("st_x", String.valueOf(b.x_)); bricknode.setAttribute("st_y", String.valueOf(b.y_)); bricknode.setAttribute("st_z", String.valueOf(b.z_)); bricknode.setAttribute("width", String.valueOf(b.w_)); bricknode.setAttribute("height", String.valueOf(b.h_)); bricknode.setAttribute("depth", String.valueOf(b.d_)); brksnode.appendChild(bricknode); Element tboxnode = doc.createElement("tbox"); tboxnode.setAttribute("x0", String.valueOf(b.tx0_)); tboxnode.setAttribute("y0", String.valueOf(b.ty0_)); tboxnode.setAttribute("z0", String.valueOf(b.tz0_)); tboxnode.setAttribute("x1", String.valueOf(b.tx1_)); tboxnode.setAttribute("y1", String.valueOf(b.ty1_)); tboxnode.setAttribute("z1", String.valueOf(b.tz1_)); bricknode.appendChild(tboxnode); Element bboxnode = doc.createElement("bbox"); bboxnode.setAttribute("x0", String.valueOf(b.bx0_)); bboxnode.setAttribute("y0", String.valueOf(b.by0_)); bboxnode.setAttribute("z0", String.valueOf(b.bz0_)); bboxnode.setAttribute("x1", String.valueOf(b.bx1_)); bboxnode.setAttribute("y1", String.valueOf(b.by1_)); bboxnode.setAttribute("z1", String.valueOf(b.bz1_)); bricknode.appendChild(bboxnode); } if (l < lv - 1) { imp = WindowManager.getImage(lvImgTitle.get(l + 1)); int[] newdims = imp.getDimensions(); imageW = newdims[0]; imageH = newdims[1]; imageD = newdims[3]; xspc = orgxspc * ((double) orgW / (double) imageW); yspc = orgyspc * ((double) orgH / (double) imageH); zspc = orgzspc * ((double) orgD / (double) imageD); bdepth = imp.getBitDepth(); } } File newXMLfile = new File(directory + basename + ".vvd"); writeXML(newXMLfile, doc); for (int l = 1; l < lv; l++) { imp = WindowManager.getImage(lvImgTitle.get(l)); imp.changes = false; imp.close(); } }
/** Ask for parameters and then execute. */ public void run(String arg) { // 1 - Obtain the currently active image: ImagePlus imp = IJ.getImage(); if (null == imp) { IJ.showMessage("There must be at least one image open"); return; } if (imp.getBitDepth() != 8) { IJ.showMessage("Error", "Only 8-bit images are supported"); return; } // 2 - Ask for parameters: GenericDialog gd = new GenericDialog("Auto Local Threshold"); String[] methods = { "Try all", "Bernsen", "Contrast", "Mean", "Median", "MidGrey", "Niblack", "Otsu", "Phansalkar", "Sauvola" }; gd.addMessage("Auto Local Threshold v1.5"); gd.addChoice("Method", methods, methods[0]); gd.addNumericField("Radius", 15, 0); gd.addMessage("Special paramters (if different from default)"); gd.addNumericField("Parameter_1", 0, 0); gd.addNumericField("Parameter_2", 0, 0); gd.addCheckbox("White objects on black background", true); if (imp.getStackSize() > 1) { gd.addCheckbox("Stack", false); } gd.addMessage("Thresholded result is always shown in white [255]."); gd.showDialog(); if (gd.wasCanceled()) return; // 3 - Retrieve parameters from the dialog String myMethod = gd.getNextChoice(); int radius = (int) gd.getNextNumber(); double par1 = (double) gd.getNextNumber(); double par2 = (double) gd.getNextNumber(); boolean doIwhite = gd.getNextBoolean(); boolean doIstack = false; int stackSize = imp.getStackSize(); if (stackSize > 1) doIstack = gd.getNextBoolean(); // 4 - Execute! // long start = System.currentTimeMillis(); if (myMethod.equals("Try all")) { ImageProcessor ip = imp.getProcessor(); int xe = ip.getWidth(); int ye = ip.getHeight(); int ml = methods.length; ImagePlus imp2, imp3; ImageStack tstack = null, stackNew; if (stackSize > 1 && doIstack) { boolean doItAnyway = true; if (stackSize > 25) { YesNoCancelDialog d = new YesNoCancelDialog( IJ.getInstance(), "Auto Local Threshold", "You might run out of memory.\n \nDisplay " + stackSize + " slices?\n \n \'No\' will process without display and\noutput results to the log window."); if (!d.yesPressed()) { // doIlog=true; //will show in the log window doItAnyway = false; } if (d.cancelPressed()) return; } for (int j = 1; j <= stackSize; j++) { imp.setSlice(j); ip = imp.getProcessor(); tstack = new ImageStack(xe, ye); for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate()); imp2 = new ImagePlus("Auto Threshold", tstack); imp2.updateAndDraw(); for (int k = 1; k < ml; k++) { imp2.setSlice(k); Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite); } // if (doItAnyway){ CanvasResizer cr = new CanvasResizer(); stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1); imp3 = new ImagePlus("Auto Threshold", stackNew); imp3.updateAndDraw(); MontageMaker mm = new MontageMaker(); mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); // 3 columns and 3 rows } imp.setSlice(1); // if (doItAnyway) IJ.run("Images to Stack", "method=[Copy (center)] title=Montage"); return; } else { // single image try all tstack = new ImageStack(xe, ye); for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate()); imp2 = new ImagePlus("Auto Threshold", tstack); imp2.updateAndDraw(); for (int k = 1; k < ml; k++) { imp2.setSlice(k); // IJ.log("analyzing slice with "+methods[k]); Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite); } // imp2.setSlice(1); CanvasResizer cr = new CanvasResizer(); stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1); imp3 = new ImagePlus("Auto Threshold", stackNew); imp3.updateAndDraw(); MontageMaker mm = new MontageMaker(); mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); return; } } else { // selected a method if (stackSize > 1 && doIstack) { // whole stack // if (doIstackHistogram) {// one global histogram // Object[] result = exec(imp, myMethod, noWhite, noBlack, doIwhite, doIset, doIlog, // doIstackHistogram ); // } // else{ // slice by slice for (int k = 1; k <= stackSize; k++) { imp.setSlice(k); Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite); } // } imp.setSlice(1); } else { // just one slice Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite); } // 5 - If all went well, show the image: // not needed here as the source image is binarised } }
public void run(String arg) { imp = IJ.getImage(); int stackSize = imp.getStackSize(); if (imp == null) { IJ.noImage(); return; } // Make sure input image is a stack. if (stackSize == 1) { IJ.error("Z Project", "Stack required"); return; } // Check for inverting LUT. if (imp.getProcessor().isInvertedLut()) { if (!IJ.showMessageWithCancel("ZProjection", lutMessage)) return; } // Set default bounds. int channels = imp.getNChannels(); int frames = imp.getNFrames(); int slices = imp.getNSlices(); isHyperstack = imp.isHyperStack() || (ij.macro.Interpreter.isBatchMode() && ((frames > 1 && frames < stackSize) || (slices > 1 && slices < stackSize))); boolean simpleComposite = channels == stackSize; if (simpleComposite) isHyperstack = false; startSlice = 1; if (isHyperstack) { int nSlices = imp.getNSlices(); if (nSlices > 1) stopSlice = nSlices; else stopSlice = imp.getNFrames(); } else stopSlice = stackSize; // Build control dialog GenericDialog gd = buildControlDialog(startSlice, stopSlice); gd.showDialog(); if (gd.wasCanceled()) return; if (!imp.lock()) return; // exit if in use long tstart = System.currentTimeMillis(); setStartSlice((int) gd.getNextNumber()); setStopSlice((int) gd.getNextNumber()); method = gd.getNextChoiceIndex(); Prefs.set(METHOD_KEY, method); if (isHyperstack) { allTimeFrames = imp.getNFrames() > 1 && imp.getNSlices() > 1 ? gd.getNextBoolean() : false; doHyperStackProjection(allTimeFrames); } else if (imp.getType() == ImagePlus.COLOR_RGB) doRGBProjection(true); else doProjection(true); if (arg.equals("") && projImage != null) { long tstop = System.currentTimeMillis(); projImage.setCalibration(imp.getCalibration()); if (simpleComposite) IJ.run(projImage, "Grays", ""); projImage.show("ZProjector: " + IJ.d2s((tstop - tstart) / 1000.0, 2) + " seconds"); } imp.unlock(); IJ.register(ZProjector.class); return; }