public static boolean validateOverhangs(ArrayList<RGraph> graphs) {
boolean valid = true;
for (RGraph graph : graphs) {
ArrayList<RNode> queue = new ArrayList<RNode>();
HashSet<RNode> seenNodes = new HashSet<RNode>();
RNode root = graph.getRootNode();
queue.add(root);
while (!queue.isEmpty()) {
RNode current = queue.get(0);
queue.remove(0);
seenNodes.add(current);
if (!("EX".equals(current.getLOverhang()) && "SP".equals(current.getROverhang()))) {
return false;
}
ArrayList<RNode> neighbors = current.getNeighbors();
for (RNode neighbor : neighbors) {
if (!seenNodes.contains(neighbor)) {
queue.add(neighbor);
}
}
}
}
return valid;
}
/**
* First step of overhang assignment - enforce numeric place holders for overhangs, ie no overhang
* redundancy in any step *
*/
private void assignBioBricksOverhangs(
ArrayList<RGraph> optimalGraphs, HashMap<Integer, Vector> stageVectors) {
// Initialize fields that record information to save complexity for future steps
_rootBasicNodeHash = new HashMap<RNode, ArrayList<RNode>>();
HashMap<Integer, RVector> stageRVectors = new HashMap<Integer, RVector>();
for (Integer stage : stageVectors.keySet()) {
RVector vec = ClothoReader.vectorImportClotho(stageVectors.get(stage));
stageRVectors.put(stage, vec);
}
// If the stageVector hash is empty, make a new default vector
if (stageRVectors.size() == 1) {
if (stageRVectors.get(1) == null) {
stageRVectors.put(0, new RVector("EX", "SP", -1, "pSK1A2", null));
}
}
// Loop through each optimal graph and grab the root node to prime for the traversal
for (RGraph graph : optimalGraphs) {
RNode root = graph.getRootNode();
RVector vector = stageRVectors.get(root.getStage() % stageRVectors.size());
RVector rootVector = new RVector("EX", "SP", -1, vector.getName(), null);
root.setVector(rootVector);
root.setLOverhang("EX");
root.setROverhang("SP");
ArrayList<RNode> l0nodes = new ArrayList<RNode>();
_rootBasicNodeHash.put(root, l0nodes);
ArrayList<RNode> neighbors = root.getNeighbors();
assignBioBricksOverhangsHelper(root, neighbors, root, stageRVectors);
}
// Determine which nodes impact which level to form the stageDirectionAssignHash
for (RGraph graph : optimalGraphs) {
RNode root = graph.getRootNode();
ArrayList<String> rootDir = new ArrayList<String>();
ArrayList<String> direction = root.getDirection();
rootDir.addAll(direction);
ArrayList<RNode> l0Nodes = _rootBasicNodeHash.get(root);
// Determine which levels each basic node impacts
for (int i = 0; i < l0Nodes.size(); i++) {
// Determine direction of basic level 0 nodes
RNode l0Node = l0Nodes.get(i);
String l0Direction = rootDir.get(0);
if (l0Node.getComposition().size() == 1) {
ArrayList<String> l0Dir = new ArrayList<String>();
l0Dir.add(l0Direction);
l0Node.setDirection(l0Dir);
}
int size = l0Node.getDirection().size();
rootDir.subList(0, size).clear();
}
}
}
/** Determine overhang scars * */
private void assignScars(ArrayList<RGraph> optimalGraphs) {
// Loop through each optimal graph and grab the root node to prime for the traversal
for (RGraph graph : optimalGraphs) {
RNode root = graph.getRootNode();
ArrayList<RNode> children = root.getNeighbors();
assignScarsHelper(root, children);
}
}
