Пример #1
0
  /*.................................................................................................................*/
  public void processAceFileWithoutContig(
      DNAData data,
      String processedAceFilePath,
      AceFile ace,
      String geneName,
      MesquiteString fullName,
      int it,
      MesquiteString voucherCode) {
    DNAData editedData = ChromaseqUtil.getEditedData(data);
    DNAData originalData = ChromaseqUtil.getOriginalData(data);
    Taxa taxa = data.getTaxa();
    ace.processFailedContig(polyThreshold);
    ace.setNameTranslation(fileNameTranslation);

    ace.renameContigs(fullName.toString(), addFragName, geneName);
    ace.setLowQualityToLowerCase(qualThresholdForLowerCase);
    ace.writeToPropertiesFile(contigPropertiesFileBuffer, fullName.toString());
    if (truncateMixedEnds) {
      ace.trimMixedEnds(mixedEndThreshold, mixedEndWindow, qualThresholdForTrim, addPhrapFailures);
    }
    MesquiteFile.putFileContents(processedAceFilePath, ace.toString(processPolymorphisms), true);
    ace.importSequence(
        taxa,
        editedData,
        it,
        originalData,
        ChromaseqUtil.getQualityData(data),
        ChromaseqUtil.getRegistryData(data),
        singleTaxaBlock,
        processPolymorphisms,
        maxChar,
        "",
        true,
        voucherCode);
  }
Пример #2
0
  /*.................................................................................................................*/
  public void processAceFileWithContig(
      CharacterData data,
      MesquiteModule ownerModule,
      String processedAceFilePath,
      String fragmentDirPath,
      AceFile ace,
      SequenceUploader uploader,
      String geneName,
      MesquiteString fullName,
      String baseName,
      MesquiteString voucherCode,
      int it) {
    DNAData editedData = ChromaseqUtil.getEditedData(data);
    DNAData originalData = ChromaseqUtil.getOriginalData(data);
    Taxa taxa = data.getTaxa();
    ace.setNameTranslation(fileNameTranslation);
    ownerModule.log(ace.contigListForLog() + StringUtil.lineEnding());
    if (processPolymorphisms) ace.processPolys(); // creates an additional CO that has polys in it
    if (renameContigsInAceFiles) ace.renameContigs(fullName.toString(), addFragName, geneName);
    ace.setLowQualityToLowerCase(qualThresholdForLowerCase);
    ace.writeToPropertiesFile(contigPropertiesFileBuffer, fullName.toString());
    if (truncateMixedEnds)
      ace.trimMixedEnds(mixedEndThreshold, mixedEndWindow, qualThresholdForTrim, addPhrapFailures);

    /*		if (uploadResultsToDatabase && StringUtil.notEmpty(databaseURL)) {
    			uploader.uploadAceFileToServer(MesquiteXMLToLUtilities.getTOLPageDatabaseURL(databaseURL), ace, processPolymorphisms, qualThresholdForTrim);
    		}
    */
    System.out.println("\n\nfasta file name: " + baseName + " ace file: " + ace);
    MesquiteFile.putFileContents(
        fragmentDirPath
            + MesquiteFile.fileSeparator
            + ChromaseqUtil.processedFastaFolder
            + MesquiteFile.fileSeparator
            + baseName
            + ".fas",
        ace.toFASTAString(processPolymorphisms, qualThresholdForTrim),
        true);
    MesquiteFile.putFileContents(processedAceFilePath, ace.toString(processPolymorphisms), true);
    ace.importSequence(
        taxa,
        editedData,
        it,
        originalData,
        ChromaseqUtil.getQualityData(data),
        ChromaseqUtil.getRegistryData(data),
        singleTaxaBlock,
        processPolymorphisms,
        maxChar,
        " contig ",
        false,
        voucherCode);
  }
Пример #3
0
  /*.................................................................................................................*/
  public void reprocessAceFileDirectory(
      MesquiteFile file, MesquiteModule ownerModule, DNAData data, int it) {
    if (data == null || file == null) return;
    String aceFileDirectoryPath =
        ChromaseqUtil.getAceFileDirectory(file.getDirectoryName(), ownerModule, data, it);
    File aceFileDirectory = new File(aceFileDirectoryPath);
    boolean addFragName = false; // control of this?
    int currentRead = -1;
    String dataFilePath = MesquiteFile.composePath(data.getProject().getHomeDirectoryName(), "");
    boolean addingPhrapFailures = false;
    AceFile ace = null;
    MesquiteProject project = data.getProject();
    if (project == null) return;
    String processedAceFilePath = "";
    MesquiteString fullName = null;
    MesquiteString voucherCode = null;
    String geneName = ChromaseqUtil.getGeneName(data);
    if (aceFileDirectory.isDirectory()) {
      int numPhdFiles = getNumPhdFilesInDirectory(aceFileDirectory, aceFileDirectoryPath);
      fileNameTranslation = new String[5][numPhdFiles];
      fillNameTranslation(data, it, numPhdFiles);

      String[] files = aceFileDirectory.list();
      for (int i = 0;
          i < files.length;
          i++) { // going through the folders and finding the ace files
        if (files[i] != null) {
          String filePath = aceFileDirectoryPath + MesquiteFile.fileSeparator + files[i];
          String infoFilePath =
              aceFileDirectoryPath + MesquiteFile.fileSeparator + ChromaseqUtil.infoFileName;
          File cFile = new File(filePath);
          if (cFile.exists()) {
            if (!cFile.isDirectory()) {
              if (files[i].endsWith(ChromaseqUtil.processedACESuffix + ".ace")) {
                // don't do anything
              } else if (files[i].endsWith(".ace")
                  && !files[i].startsWith(".")
                  && !addingPhrapFailures) {
                ownerModule.logln("Processing ACE file: " + files[i]);

                String baseName =
                    files[i].substring(
                        0, files[i].length() - 4); // this is the name of the sequence
                processedAceFilePath =
                    aceFileDirectoryPath
                        + MesquiteFile.fileSeparator
                        + baseName
                        + ChromaseqUtil.processedACESuffix
                        + ".ace";

                ace =
                    new AceFile(
                        filePath,
                        processedAceFilePath,
                        dataFilePath,
                        dataFilePath,
                        ownerModule,
                        processPolymorphisms,
                        polyThreshold,
                        false);
                if (ace == null) return;

                ace.setBaseName(baseName);
                fullName = new MesquiteString(baseName);
                voucherCode = new MesquiteString();
                ChromaseqInfoFile.processInfoFile(infoFilePath, fullName, voucherCode);
                String fragmentDirPath =
                    StringUtil.getAllButLastItem(
                        StringUtil.getAllButLastItem(
                            aceFileDirectoryPath, MesquiteFile.fileSeparator),
                        MesquiteFile.fileSeparator);
                ace.setLongSequenceName(fullName.toString());
                if (ace.getNumContigs() >= 1) {
                  processAceFileWithContig(
                      data,
                      ownerModule,
                      processedAceFilePath,
                      fragmentDirPath,
                      ace,
                      null,
                      geneName,
                      fullName,
                      baseName,
                      voucherCode,
                      it);
                } else {
                  ownerModule.logln("   ACE file contains no contigs!");
                  if (project != null) {
                    addingPhrapFailures = true;
                    i = 0;
                    ace.createEmptyContigs(
                        MesquiteFile.numFilesEndingWith(
                            aceFileDirectoryPath, files, ".phd.1")); // create an empty contig
                    ace.renameContigs(fullName.toString(), addFragName, geneName);
                  }
                }

                if (!addingPhrapFailures) ace.dispose();
              } else if (files[i].endsWith(".phd.1") && addingPhrapFailures) {
                ownerModule.logln("   Importing single-read Phred file " + files[i]);
                currentRead++;
                ace.addPhdFileAsSingleReadInContig(
                    currentRead,
                    aceFileDirectoryPath,
                    files[i],
                    processPolymorphisms,
                    polyThreshold);
              }
            }
          }
        }
      }
    }

    if (addingPhrapFailures && ace != null) { // have to process AceFile that we have manually made
      MesquiteFile.putFileContents(processedAceFilePath, ace.toString(processPolymorphisms), true);
      if (project != null) {
        processAceFileWithoutContig(
            data, processedAceFilePath, ace, geneName, fullName, it, voucherCode);
      }
      ace.dispose();
    }
  }