boolean eraseOutsideRoi(ImageProcessor ip, Rectangle r, ImageProcessor mask) {
int width = ip.getWidth();
int height = ip.getHeight();
ip.setRoi(r);
if (excludeEdgeParticles && polygon != null) {
ImageStatistics stats = ImageStatistics.getStatistics(ip, MIN_MAX, null);
if (fillColor >= stats.min && fillColor <= stats.max) {
double replaceColor = level1 - 1.0;
if (replaceColor < 0.0 || replaceColor == fillColor) {
replaceColor = level2 + 1.0;
int maxColor = imageType == BYTE ? 255 : 65535;
if (replaceColor > maxColor || replaceColor == fillColor) {
IJ.error("Particle Analyzer", "Unable to remove edge particles");
return false;
}
}
for (int y = minY; y < maxY; y++) {
for (int x = minX; x < maxX; x++) {
int v = ip.getPixel(x, y);
if (v == fillColor) ip.putPixel(x, y, (int) replaceColor);
}
}
}
}
ip.setValue(fillColor);
if (mask != null) {
mask = mask.duplicate();
mask.invert();
ip.fill(mask);
}
ip.setRoi(0, 0, r.x, height);
ip.fill();
ip.setRoi(r.x, 0, r.width, r.y);
ip.fill();
ip.setRoi(r.x, r.y + r.height, r.width, height - (r.y + r.height));
ip.fill();
ip.setRoi(r.x + r.width, 0, width - (r.x + r.width), height);
ip.fill();
ip.resetRoi();
// IJ.log("erase: "+fillColor+" "+level1+" "+level2+" "+excludeEdgeParticles);
// (new ImagePlus("ip2", ip.duplicate())).show();
return true;
}
/**
* Performs particle analysis on the specified ImagePlus and ImageProcessor. Returns false if
* there is an error.
*/
public boolean analyze(ImagePlus imp, ImageProcessor ip) {
if (this.imp == null) this.imp = imp;
showResults = (options & SHOW_RESULTS) != 0;
excludeEdgeParticles = (options & EXCLUDE_EDGE_PARTICLES) != 0;
resetCounter = (options & CLEAR_WORKSHEET) != 0;
showProgress = (options & SHOW_PROGRESS) != 0;
floodFill = (options & INCLUDE_HOLES) == 0;
recordStarts = (options & RECORD_STARTS) != 0;
addToManager = (options & ADD_TO_MANAGER) != 0;
displaySummary = (options & DISPLAY_SUMMARY) != 0;
inSituShow = (options & IN_SITU_SHOW) != 0;
outputImage = null;
ip.snapshot();
ip.setProgressBar(null);
if (Analyzer.isRedirectImage()) {
redirectImp = Analyzer.getRedirectImage(imp);
if (redirectImp == null) return false;
int depth = redirectImp.getStackSize();
if (depth > 1 && depth == imp.getStackSize()) {
ImageStack redirectStack = redirectImp.getStack();
redirectIP = redirectStack.getProcessor(imp.getCurrentSlice());
} else redirectIP = redirectImp.getProcessor();
} else if (imp.getType() == ImagePlus.COLOR_RGB) {
ImagePlus original = (ImagePlus) imp.getProperty("OriginalImage");
if (original != null
&& original.getWidth() == imp.getWidth()
&& original.getHeight() == imp.getHeight()) {
redirectImp = original;
redirectIP = original.getProcessor();
}
}
if (!setThresholdLevels(imp, ip)) return false;
width = ip.getWidth();
height = ip.getHeight();
if (!(showChoice == NOTHING || showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS)) {
blackBackground = Prefs.blackBackground && inSituShow;
if (slice == 1) outlines = new ImageStack(width, height);
if (showChoice == ROI_MASKS) drawIP = new ShortProcessor(width, height);
else drawIP = new ByteProcessor(width, height);
drawIP.setLineWidth(lineWidth);
if (showChoice == ROI_MASKS) {
} // Place holder for now...
else if (showChoice == MASKS && !blackBackground) drawIP.invertLut();
else if (showChoice == OUTLINES) {
if (!inSituShow) {
if (customLut == null) makeCustomLut();
drawIP.setColorModel(customLut);
}
drawIP.setFont(new Font("SansSerif", Font.PLAIN, fontSize));
if (fontSize > 12 && inSituShow) drawIP.setAntialiasedText(true);
}
outlines.addSlice(null, drawIP);
if (showChoice == ROI_MASKS || blackBackground) {
drawIP.setColor(Color.black);
drawIP.fill();
drawIP.setColor(Color.white);
} else {
drawIP.setColor(Color.white);
drawIP.fill();
drawIP.setColor(Color.black);
}
}
calibration = redirectImp != null ? redirectImp.getCalibration() : imp.getCalibration();
if (rt == null) {
rt = Analyzer.getResultsTable();
analyzer = new Analyzer(imp);
} else analyzer = new Analyzer(imp, measurements, rt);
if (resetCounter && slice == 1) {
if (!Analyzer.resetCounter()) return false;
}
beginningCount = Analyzer.getCounter();
byte[] pixels = null;
if (ip instanceof ByteProcessor) pixels = (byte[]) ip.getPixels();
if (r == null) {
r = ip.getRoi();
mask = ip.getMask();
if (displaySummary) {
if (mask != null) totalArea = ImageStatistics.getStatistics(ip, AREA, calibration).area;
else totalArea = r.width * calibration.pixelWidth * r.height * calibration.pixelHeight;
}
}
minX = r.x;
maxX = r.x + r.width;
minY = r.y;
maxY = r.y + r.height;
if (r.width < width || r.height < height || mask != null) {
if (!eraseOutsideRoi(ip, r, mask)) return false;
}
int offset;
double value;
int inc = Math.max(r.height / 25, 1);
int mi = 0;
ImageWindow win = imp.getWindow();
if (win != null) win.running = true;
if (measurements == 0) measurements = Analyzer.getMeasurements();
if (showChoice == ELLIPSES) measurements |= ELLIPSE;
measurements &= ~LIMIT; // ignore "Limit to Threshold"
roiNeedsImage =
(measurements & PERIMETER) != 0
|| (measurements & SHAPE_DESCRIPTORS) != 0
|| (measurements & FERET) != 0;
particleCount = 0;
wand = new Wand(ip);
pf = new PolygonFiller();
if (floodFill) {
ImageProcessor ipf = ip.duplicate();
ipf.setValue(fillColor);
ff = new FloodFiller(ipf);
}
roiType = Wand.allPoints() ? Roi.FREEROI : Roi.TRACED_ROI;
for (int y = r.y; y < (r.y + r.height); y++) {
offset = y * width;
for (int x = r.x; x < (r.x + r.width); x++) {
if (pixels != null) value = pixels[offset + x] & 255;
else if (imageType == SHORT) value = ip.getPixel(x, y);
else value = ip.getPixelValue(x, y);
if (value >= level1 && value <= level2) analyzeParticle(x, y, imp, ip);
}
if (showProgress && ((y % inc) == 0)) IJ.showProgress((double) (y - r.y) / r.height);
if (win != null) canceled = !win.running;
if (canceled) {
Macro.abort();
break;
}
}
if (showProgress) IJ.showProgress(1.0);
if (showResults) rt.updateResults();
imp.killRoi();
ip.resetRoi();
ip.reset();
if (displaySummary && IJ.getInstance() != null) updateSliceSummary();
if (addToManager && roiManager != null) roiManager.setEditMode(imp, true);
maxParticleCount = (particleCount > maxParticleCount) ? particleCount : maxParticleCount;
totalCount += particleCount;
if (!canceled) showResults();
return true;
}
/*------------------------------------------------------------------*/
void doIt(ImageProcessor ip) {
int width = ip.getWidth();
int height = ip.getHeight();
double hLine[] = new double[width];
double vLine[] = new double[height];
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
switch (operation) {
case GRADIENT_MAGNITUDE:
{
ImageProcessor h = ip.duplicate();
ImageProcessor v = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsH = (float[]) h.getPixels();
float[] floatPixelsV = (float[]) v.getPixels();
getHorizontalGradient(h, FLT_EPSILON);
getVerticalGradient(v, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
Math.sqrt(
floatPixelsH[k] * floatPixelsH[k] + floatPixelsV[k] * floatPixelsV[k]);
}
stepProgressBar();
}
}
break;
case GRADIENT_DIRECTION:
{
ImageProcessor h = ip.duplicate();
ImageProcessor v = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsH = (float[]) h.getPixels();
float[] floatPixelsV = (float[]) v.getPixels();
getHorizontalGradient(h, FLT_EPSILON);
getVerticalGradient(v, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] = (float) Math.atan2(floatPixelsH[k], floatPixelsV[k]);
}
stepProgressBar();
}
}
break;
case LAPLACIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] = (float) (floatPixelsHH[k] + floatPixelsVV[k]);
}
stepProgressBar();
}
}
break;
case LARGEST_HESSIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
(0.5
* (floatPixelsHH[k]
+ floatPixelsVV[k]
+ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
stepProgressBar();
}
}
break;
case SMALLEST_HESSIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
(0.5
* (floatPixelsHH[k]
+ floatPixelsVV[k]
- Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
stepProgressBar();
}
}
break;
case HESSIAN_ORIENTATION:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
if (floatPixelsHV[k] < 0.0) {
floatPixels[k] =
(float)
(-0.5
* Math.acos(
(floatPixelsHH[k] - floatPixelsVV[k])
/ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
} else {
floatPixels[k] =
(float)
(0.5
* Math.acos(
(floatPixelsHH[k] - floatPixelsVV[k])
/ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
}
stepProgressBar();
}
}
break;
default:
throw new IllegalArgumentException("Invalid operation");
}
ip.resetMinAndMax();
imp.updateAndDraw();
} /* end doIt */