Exemplo n.º 1
0
  /** Called from io/Opener.java. */
  public void run(String path) {
    if (path.equals("")) return;
    File theFile = new File(path);
    String directory = theFile.getParent();
    String fileName = theFile.getName();
    if (directory == null) directory = "";

    // Try and recognise file type and load the file if recognised
    ImagePlus imp = openImage(directory, fileName, path);
    if (imp == null) {
      IJ.showStatus("");
      return; // failed to load file or plugin has opened and displayed it
    }
    ImageStack stack = imp.getStack();
    // get the title from the stack (falling back to the fileName)
    String title = imp.getTitle().equals("") ? fileName : imp.getTitle();
    // set the stack of this HandleExtraFileTypes object
    // to that attached to the ImagePlus object returned by openImage()
    setStack(title, stack);
    // copy over the calibration info since it doesn't come with the ImageProcessor
    setCalibration(imp.getCalibration());
    // also copy the Show Info field over if it exists
    if (imp.getProperty("Info") != null) setProperty("Info", imp.getProperty("Info"));
    // copy the FileInfo
    setFileInfo(imp.getOriginalFileInfo());
    // copy dimensions
    if (IJ.getVersion().compareTo("1.38s") >= 0)
      setDimensions(imp.getNChannels(), imp.getNSlices(), imp.getNFrames());
    if (IJ.getVersion().compareTo("1.41o") >= 0) setOpenAsHyperStack(imp.getOpenAsHyperStack());
  }
Exemplo n.º 2
0
  public void run(String arg) {
    ImagePlus imp = WindowManager.getCurrentImage();
    if (imp == null) {
      IJ.noImage();
      return;
    }

    ImageStack stack1 = imp.getStack();
    String fileName = imp.getTitle();
    int endslice = stack1.getSize();

    ImagePlus imp2 = duplicateStack(imp);
    imp2.show();
    String duplicateName = imp2.getTitle();
    // IJ.showMessage("Box",fileName);
    ImageStack stack2 = imp2.getStack();
    stack1.deleteSlice(1);
    stack2.deleteSlice(endslice);

    String calculatorstring =
        ("image1='"
            + fileName
            + "' operation=Subtract image2="
            + imp2.getTitle()
            + " create stack");

    IJ.run("Image Calculator...", calculatorstring);
    ImagePlus imp3 = WindowManager.getCurrentImage();
    imp3.setTitle(fileName + " DeltaF up");
    imp2.getWindow().close();
    imp.getWindow().close();
  }
Exemplo n.º 3
0
  public boolean beadCalibration3d() {
    imp = IJ.getImage();
    if (imp == null) {
      IJ.noImage();
      return false;
    } else if (imp.getStackSize() == 1) {
      IJ.error("Stack required");
      return false;
    } else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
      // In order to support 32bit images, pict[] must be changed to float[], and  getPixel(x, y);
      // requires a Float.intBitsToFloat() conversion
      IJ.error("8 or 16 bit greyscale image required");
      return false;
    }
    width = imp.getWidth();
    height = imp.getHeight();
    nslices = imp.getStackSize();
    imtitle = imp.getTitle();

    models[0] = "*None*";
    models[1] = "line";
    models[2] = "2nd degree polynomial";
    models[3] = "3rd degree polynomial";
    models[4] = "4th degree polynomial";

    GenericDialog gd = new GenericDialog("3D PALM calibration");
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
    gd.addNumericField(
        "Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
    gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
    gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
    gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
    gd.addCheckbox(
        "Show divergence of bead positions against model",
        prefs.get("QuickPALM.3Dcal_showDivergence", false));
    gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
    gd.addMessage("\n\nDon't forget to save the table in the end...");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.pthrsh", pthrsh * 100);
    cal_z = gd.getNextNumber();
    prefs.set("QuickPALM.z-step", cal_z);
    window = (int) gd.getNextNumber();
    prefs.set("QuickPALM.window", window);
    model = gd.getNextChoice();
    prefs.set("QuickPALM.model", model);
    part_divergence = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
    part_extrainfo = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
    return true;
  }
Exemplo n.º 4
0
  private boolean showDialog() {
    String[] types = {"RAW", "JPEG", "ZLIB"};
    GenericDialog gd = new GenericDialog("Generate Bricks");
    gd.addChoice("FileType", types, filetype);
    gd.addNumericField("JPEG quality", jpeg_quality, 0);
    gd.addNumericField("Max file size (MB)", bdsizelimit, 0);

    int[] wlist = WindowManager.getIDList();
    if (wlist == null) return false;

    String[] titles = new String[wlist.length];
    for (int i = 0; i < wlist.length; i++) titles[i] = "";

    int tnum = 0;
    for (int i = 0; i < wlist.length; i++) {
      ImagePlus imp = WindowManager.getImage(wlist[i]);
      if (imp != null) {
        titles[tnum] = imp.getTitle();
        tnum++;
      }
    }
    gd.addChoice("Source image: ", titles, titles[0]);

    gd.showDialog();
    if (gd.wasCanceled()) return false;

    filetype = types[gd.getNextChoiceIndex()];
    jpeg_quality = (int) gd.getNextNumber();
    if (jpeg_quality > 100) jpeg_quality = 100;
    if (jpeg_quality < 0) jpeg_quality = 0;
    bdsizelimit = (int) gd.getNextNumber();

    int id = gd.getNextChoiceIndex();
    lvImgTitle = new ArrayList<String>();
    lvImgTitle.add(titles[id]);

    Prefs.set("filetype.string", filetype);
    Prefs.set("jpeg_quality.int", jpeg_quality);
    Prefs.set("bdsizelimit.int", bdsizelimit);

    return true;
  }
Exemplo n.º 5
0
  public void Calc_5Fr(ImagePlus imp1, ImagePlus imp2) {
    if (imp1.getType() != imp2.getType()) {
      error();
      return;
    }
    if (imp1.getType() == 0) { // getType returns 0 for 8-bit, 1 for 16-bit
      bitDepth = "8-bit";
      Prefs.set("ps.bitDepth", bitDepth);
    } else {
      bitDepth = "16-bit";
      Prefs.set("ps.bitDepth", bitDepth);
    }
    int width = imp1.getWidth();
    int height = imp1.getHeight();
    if (width != imp2.getWidth() || height != imp2.getHeight()) {
      error();
      return;
    }

    ImageStack stack1 = imp1.getStack();
    //		if (bgStackTitle != "NoBg") ImageStack stack2 = imp2.getStack();
    ImageStack stack2 = imp2.getStack();

    ImageProcessor ip = imp1.getProcessor();
    int dimension = width * height;
    byte[] pixB;
    short[] pixS;
    float[][] pixF = new float[5][dimension];
    float[][] pixFBg = new float[5][dimension];

    float a;
    float b;
    float den;
    float aSmp;
    float bSmp;
    float denSmp;
    float aBg;
    float bBg;
    float denBg;
    float retF;
    float azimF;

    byte[] retB = new byte[dimension];
    short[] retS = new short[dimension];
    byte[] azimB = new byte[dimension];
    short[] azimS = new short[dimension];
    // Derived Variables:
    float swingAngle = 2f * (float) Math.PI * swing;
    float tanSwingAngleDiv2 = (float) Math.tan(swingAngle / 2.f);
    float tanSwingAngleDiv2DivSqrt2 = (float) (Math.tan(swingAngle / 2.f) / Math.sqrt(2));
    float wavelengthDiv2Pi = wavelength / (2f * (float) Math.PI);

    // get the pixels of each slice in the stack and convert to float
    for (int i = 0; i < 5; i++) {
      if (bitDepth == "8-bit") {
        pixB = (byte[]) stack1.getPixels(i + 3);
        for (int j = 0; j < dimension; j++) pixF[i][j] = 0xff & pixB[j];
        if (bgStackTitle != "NoBg") {
          pixB = (byte[]) stack2.getPixels(i + 3);
          for (int j = 0; j < dimension; j++) pixFBg[i][j] = 0xff & pixB[j];
        }
      } else {
        pixS = (short[]) stack1.getPixels(i + 3);
        for (int j = 0; j < dimension; j++) pixF[i][j] = (float) pixS[j];
        if (bgStackTitle != "NoBg") {
          pixS = (short[]) stack2.getPixels(i + 3);
          for (int j = 0; j < dimension; j++) pixFBg[i][j] = (float) pixS[j];
        }
      }
    }

    // Algorithm
    // terms a and b
    for (int j = 0; j < dimension; j++) {
      denSmp = (pixF[1][j] + pixF[2][j] + pixF[3][j] + pixF[4][j] - 4 * pixF[0][j]) / 2;
      denBg = denSmp;
      a = (pixF[4][j] - pixF[1][j]);
      aSmp = a;
      aBg = a;
      b = (pixF[2][j] - pixF[3][j]);
      bSmp = b;
      bBg = b;
      if (bgStackTitle != "NoBg") {
        denBg = (pixFBg[1][j] + pixFBg[2][j] + pixFBg[3][j] + pixFBg[4][j] - 4 * pixFBg[0][j]) / 2;
        aBg = pixFBg[4][j] - pixFBg[1][j];
        bBg = pixFBg[2][j] - pixFBg[3][j];
      }
      // Special case of sample retardance half wave, denSmp = 0
      if (denSmp == 0) {
        retF = (float) wavelength / 4;
        azimF =
            (float) (a == 0 & b == 0 ? 0 : (azimRef + 90 + 90 * Math.atan2(a, b) / Math.PI) % 180);
      } else {
        // Retardance, the background correction can be improved by separately considering sample
        // retardance values larger than a quarter wave
        if (bgStackTitle != "NoBg") {
          a = aSmp / denSmp - aBg / denBg;
          b = bSmp / denSmp - bBg / denBg;
        } else {
          a = aSmp / denSmp;
          b = bSmp / denSmp;
        }
        retF = (float) Math.atan(tanSwingAngleDiv2 * Math.sqrt(a * a + b * b));
        if (denSmp < 0) retF = (float) Math.PI - retF;
        retF = retF * wavelengthDiv2Pi; // convert to nm
        if (retF > retCeiling) retF = retCeiling;

        // Orientation
        if ((bgStackTitle == "NoBg") || ((bgStackTitle != "NoBg") && (Math.abs(denSmp) < 1))) {
          a = aSmp;
          b = bSmp;
        }
        azimF =
            (float) (a == 0 & b == 0 ? 0 : (azimRef + 90 + 90 * Math.atan2(a, b) / Math.PI) % 180);
      }
      if (bitDepth == "8-bit") retB[j] = (byte) (((int) (255 * retF / retCeiling)) & 0xff);
      else retS[j] = (short) (4095 * retF / retCeiling);
      if (mirror == "Yes") azimF = 180 - azimF;
      if (bitDepth == "8-bit") azimB[j] = (byte) (((int) azimF) & 0xff);
      else azimS[j] = (short) (azimF * 10f);
    }
    // show the resulting images in slice 1 and 2
    imp1.setSlice(3);
    if (bitDepth == "8-bit") {
      stack1.setPixels(retB, 1);
      stack1.setPixels(azimB, 2);
    } else {
      stack1.setPixels(retS, 1);
      stack1.setPixels(azimS, 2);
    }
    imp1.setSlice(1);
    IJ.selectWindow(imp1.getTitle());

    Prefs.set("ps.sampleStackTitle", sampleStackTitle);
    Prefs.set("ps.bgStackTitle", bgStackTitle);
    Prefs.set("ps.mirror", mirror);
    Prefs.set("ps.wavelength", wavelength);
    Prefs.set("ps.swing", swing);
    Prefs.set("ps.retCeiling", retCeiling);
    Prefs.set("ps.azimRef", azimRef);
    Prefs.savePreferences();
  }
Exemplo n.º 6
0
  public boolean showDialog() {

    String bgChoice = "NoBg";
    int[] wList = WindowManager.getIDList();
    if (wList == null) {
      IJ.noImage();
      return false;
    }

    String[] sampleTitles = new String[wList.length];
    for (int i = 0; i < wList.length; i++) {
      ImagePlus imp = WindowManager.getImage(wList[i]);
      sampleTitles[i] = imp != null ? imp.getTitle() : "";
    }
    String[] bgTitles = new String[wList.length + 1];
    bgTitles[0] = "NoBg";
    for (int i = 1; i < wList.length + 1; i++) {
      bgTitles[i] = sampleTitles[i - 1];
    }
    for (int i = 1; i < (wList.length + 1); i++) {
      if (bgTitles[i] == bgStackTitle) bgChoice = bgStackTitle;
    }
    String sampleChoice = sampleTitles[0];
    for (int i = 1; i < (wList.length); i++) {
      if (sampleTitles[i] == sampleStackTitle) sampleChoice = sampleStackTitle;
    }

    String[] mirrors = new String[2];
    mirrors[0] = "No";
    mirrors[1] = "Yes";

    GenericDialog gd = new GenericDialog("PolScope 5Frame Calc");
    gd.addChoice("Sample:", sampleTitles, sampleChoice);
    gd.addChoice("Background:", bgTitles, bgChoice);
    gd.addChoice("Mirror:", mirrors, mirror);
    gd.addNumericField("Wavelength: ", wavelength, 1, 8, " nm");
    gd.addNumericField("Swing: ", swing, 3, 8, " wavelength");
    gd.addNumericField("Ret. Ceiling: ", retCeiling, 1, 8, " nm");
    gd.addNumericField("Orient. Ref.: ", azimRef, 1, 8, " degree");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    int index1 = gd.getNextChoiceIndex();
    int index2 = gd.getNextChoiceIndex();
    int index3 = gd.getNextChoiceIndex();
    wavelength = (float) gd.getNextNumber();
    swing = (float) gd.getNextNumber();
    retCeiling = (float) gd.getNextNumber();
    azimRef = (float) gd.getNextNumber();

    imp1 = WindowManager.getImage(wList[index1]);
    sampleStackTitle = sampleTitles[index1];
    bgStackTitle = bgTitles[index2];
    if (bgStackTitle == "NoBg") {
      imp2 =
          WindowManager.getImage(
              wList[
                  index1]); // only to assign a valid ImagePlus to imp2 which is not used when NoBg
    } else {
      imp2 = WindowManager.getImage(wList[index2 - 1]);
    }
    mirror = mirrors[index3];

    return true;
  }
Exemplo n.º 7
0
 public void itemStateChanged(ItemEvent e) {
   ImagePlus imp = WindowManager.getCurrentImage();
   if (imp == null) return;
   if (!imp.isComposite()) {
     int channels = imp.getNChannels();
     if (channels == 1 && imp.getStackSize() <= 4) channels = imp.getStackSize();
     if (imp.getBitDepth() == 24 || (channels > 1 && channels < CompositeImage.MAX_CHANNELS)) {
       GenericDialog gd = new GenericDialog(imp.getTitle());
       gd.addMessage("Convert to multi-channel composite image?");
       gd.showDialog();
       if (gd.wasCanceled()) return;
       else IJ.doCommand("Make Composite");
     } else {
       IJ.error(
           "Channels",
           "A composite image is required (e.g., "
               + moreLabel
               + " Open HeLa Cells),\nor create one using "
               + moreLabel
               + " Make Composite.");
       return;
     }
   }
   if (!imp.isComposite()) return;
   CompositeImage ci = (CompositeImage) imp;
   Object source = e.getSource();
   if (source == choice) {
     int index = ((Choice) source).getSelectedIndex();
     switch (index) {
       case 0:
         ci.setMode(IJ.COMPOSITE);
         break;
       case 1:
         ci.setMode(IJ.COLOR);
         break;
       case 2:
         ci.setMode(IJ.GRAYSCALE);
         break;
     }
     ci.updateAndDraw();
     if (Recorder.record) {
       String mode = null;
       switch (index) {
         case 0:
           mode = "composite";
           break;
         case 1:
           mode = "color";
           break;
         case 2:
           mode = "grayscale";
           break;
       }
       Recorder.record("Stack.setDisplayMode", mode);
     }
   } else if (source instanceof Checkbox) {
     for (int i = 0; i < checkbox.length; i++) {
       Checkbox cb = (Checkbox) source;
       if (cb == checkbox[i]) {
         if (ci.getMode() == IJ.COMPOSITE) {
           boolean[] active = ci.getActiveChannels();
           active[i] = cb.getState();
           if (Recorder.record) {
             String str = "";
             for (int c = 0; c < ci.getNChannels(); c++) str += active[c] ? "1" : "0";
             Recorder.record("Stack.setActiveChannels", str);
             Recorder.record("//Stack.toggleChannel", imp.getChannel());
           }
         } else {
           imp.setPosition(i + 1, imp.getSlice(), imp.getFrame());
           if (Recorder.record) Recorder.record("Stack.setChannel", i + 1);
         }
         ci.updateAndDraw();
         return;
       }
     }
   }
 }