Exemplo n.º 1
0
 void createEllipse(ImagePlus imp) {
   IJ.showStatus("Fitting ellipse");
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("Fit Ellipse");
     return;
   }
   if (roi.isLine()) {
     IJ.error("Fit Ellipse", "\"Fit Ellipse\" does not work with line selections");
     return;
   }
   ImageProcessor ip = imp.getProcessor();
   ip.setRoi(roi);
   int options = Measurements.CENTROID + Measurements.ELLIPSE;
   ImageStatistics stats = ImageStatistics.getStatistics(ip, options, null);
   double dx = stats.major * Math.cos(stats.angle / 180.0 * Math.PI) / 2.0;
   double dy = -stats.major * Math.sin(stats.angle / 180.0 * Math.PI) / 2.0;
   double x1 = stats.xCentroid - dx;
   double x2 = stats.xCentroid + dx;
   double y1 = stats.yCentroid - dy;
   double y2 = stats.yCentroid + dy;
   double aspectRatio = stats.minor / stats.major;
   imp.killRoi();
   imp.setRoi(new EllipseRoi(x1, y1, x2, y2, aspectRatio));
 }
Exemplo n.º 2
0
 void interpolate() {
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("Interpolate");
     return;
   }
   if (roi.getType() == Roi.POINT) return;
   if (IJ.isMacro() && Macro.getOptions() == null) Macro.setOptions("interval=1");
   GenericDialog gd = new GenericDialog("Interpolate");
   gd.addNumericField("Interval:", 1.0, 1, 4, "pixel");
   gd.addCheckbox("Smooth", IJ.isMacro() ? false : smooth);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   double interval = gd.getNextNumber();
   smooth = gd.getNextBoolean();
   Undo.setup(Undo.ROI, imp);
   FloatPolygon poly = roi.getInterpolatedPolygon(interval, smooth);
   int t = roi.getType();
   int type = roi.isLine() ? Roi.FREELINE : Roi.FREEROI;
   if (t == Roi.POLYGON && interval > 1.0) type = Roi.POLYGON;
   if ((t == Roi.RECTANGLE || t == Roi.OVAL || t == Roi.FREEROI) && interval >= 5.0)
     type = Roi.POLYGON;
   if ((t == Roi.LINE || t == Roi.FREELINE) && interval >= 5.0) type = Roi.POLYLINE;
   if (t == Roi.POLYLINE && interval >= 1.0) type = Roi.POLYLINE;
   ImageCanvas ic = imp.getCanvas();
   if (poly.npoints <= 150 && ic != null && ic.getMagnification() >= 12.0)
     type = roi.isLine() ? Roi.POLYLINE : Roi.POLYGON;
   Roi p = new PolygonRoi(poly, type);
   if (roi.getStroke() != null) p.setStrokeWidth(roi.getStrokeWidth());
   p.setStrokeColor(roi.getStrokeColor());
   p.setName(roi.getName());
   transferProperties(roi, p);
   imp.setRoi(p);
 }
Exemplo n.º 3
0
 void lineToArea(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null || !roi.isLine()) {
     IJ.error("Line to Area", "Line selection required");
     return;
   }
   if (roi.getType() == Roi.LINE && roi.getStrokeWidth() == 1) {
     IJ.error("Line to Area", "Straight line width must be > 1");
     return;
   }
   ImageProcessor ip2 = new ByteProcessor(imp.getWidth(), imp.getHeight());
   ip2.setColor(255);
   if (roi.getType() == Roi.LINE) ip2.fillPolygon(roi.getPolygon());
   else {
     roi.drawPixels(ip2);
     // BufferedImage bi = new BufferedImage(imp.getWidth(), imp.getHeight(),
     // BufferedImage.TYPE_BYTE_GRAY);
     // Graphics g = bi.getGraphics();
     // Roi roi2 = (Roi)roi.clone();
     // roi2.setStrokeColor(Color.white);
     // roi2.drawOverlay(g);
     // ip2 = new ByteProcessor(bi);
   }
   // new ImagePlus("ip2", ip2.duplicate()).show();
   ip2.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
   ThresholdToSelection tts = new ThresholdToSelection();
   Roi roi2 = tts.convert(ip2);
   imp.setRoi(roi2);
   Roi.previousRoi = (Roi) roi.clone();
 }
Exemplo n.º 4
0
 /** Called by ImageJ when the user selects Quit. */
 public void quit() {
   quitMacro = IJ.macroRunning();
   Thread thread = new Thread(this, "Quit");
   thread.setPriority(Thread.NORM_PRIORITY);
   thread.start();
   IJ.wait(10);
 }
Exemplo n.º 5
0
 private void openDirectory(File f, String path) {
   if (path == null) return;
   if (!(path.endsWith(File.separator) || path.endsWith("/"))) path += File.separator;
   String[] names = f.list();
   names = (new FolderOpener()).trimFileList(names);
   if (names == null) return;
   String msg = "Open all " + names.length + " images in \"" + f.getName() + "\" as a stack?";
   GenericDialog gd = new GenericDialog("Open Folder");
   gd.setInsets(10, 5, 0);
   gd.addMessage(msg);
   gd.setInsets(15, 35, 0);
   gd.addCheckbox("Convert to RGB", convertToRGB);
   gd.setInsets(0, 35, 0);
   gd.addCheckbox("Use Virtual Stack", virtualStack);
   gd.enableYesNoCancel();
   gd.showDialog();
   if (gd.wasCanceled()) return;
   if (gd.wasOKed()) {
     convertToRGB = gd.getNextBoolean();
     virtualStack = gd.getNextBoolean();
     String options = " sort";
     if (convertToRGB) options += " convert_to_rgb";
     if (virtualStack) options += " use";
     IJ.run("Image Sequence...", "open=[" + path + "]" + options);
     DirectoryChooser.setDefaultDirectory(path);
   } else {
     for (int k = 0; k < names.length; k++) {
       IJ.redirectErrorMessages();
       if (!names[k].startsWith(".")) (new Opener()).open(path + names[k]);
     }
   }
   IJ.register(DragAndDrop.class);
 }
Exemplo n.º 6
0
 void runMacro(String arg) {
   Roi roi = imp.getRoi();
   if (IJ.macroRunning()) {
     String options = Macro.getOptions();
     if (options != null
         && (options.indexOf("grid=") != -1 || options.indexOf("interpolat") != -1)) {
       IJ.run("Rotate... ", options); // run Image>Transform>Rotate
       return;
     }
   }
   if (roi == null) {
     noRoi("Rotate>Selection");
     return;
   }
   roi = (Roi) roi.clone();
   if (arg.equals("rotate")) {
     double d = Tools.parseDouble(angle);
     if (Double.isNaN(d)) angle = "15";
     String value = IJ.runMacroFile("ij.jar:RotateSelection", angle);
     if (value != null) angle = value;
   } else if (arg.equals("enlarge")) {
     String value = IJ.runMacroFile("ij.jar:EnlargeSelection", enlarge);
     if (value != null) enlarge = value;
     Roi.previousRoi = roi;
   }
 }
 public int setup(String arg, ImagePlus imp) {
   this.arg = arg;
   this.imp = imp;
   IJ.register(ParticleAnalyzer.class);
   if (imp == null) {
     IJ.noImage();
     return DONE;
   }
   if (imp.getBitDepth() == 24 && !isThresholdedRGB(imp)) {
     IJ.error(
         "Particle Analyzer",
         "RGB images must be thresholded using\n" + "Image>Adjust>Color Threshold.");
     return DONE;
   }
   if (!showDialog()) return DONE;
   int baseFlags = DOES_ALL + NO_CHANGES + NO_UNDO;
   int flags = IJ.setupDialog(imp, baseFlags);
   processStack = (flags & DOES_STACKS) != 0;
   slice = 0;
   saveRoi = imp.getRoi();
   if (saveRoi != null && saveRoi.getType() != Roi.RECTANGLE && saveRoi.isArea())
     polygon = saveRoi.getPolygon();
   imp.startTiming();
   nextFontSize = defaultFontSize;
   nextLineWidth = 1;
   return flags;
 }
Exemplo n.º 8
0
 void fitSpline() {
   Roi roi = imp.getRoi();
   if (roi == null) {
     IJ.error("Spline", "Selection required");
     return;
   }
   int type = roi.getType();
   boolean segmentedSelection = type == Roi.POLYGON || type == Roi.POLYLINE;
   if (!(segmentedSelection
       || type == Roi.FREEROI
       || type == Roi.TRACED_ROI
       || type == Roi.FREELINE)) {
     IJ.error("Spline", "Polygon or polyline selection required");
     return;
   }
   if (roi instanceof EllipseRoi) return;
   PolygonRoi p = (PolygonRoi) roi;
   if (!segmentedSelection) p = trimPolygon(p, p.getUncalibratedLength());
   String options = Macro.getOptions();
   if (options != null && options.indexOf("straighten") != -1) p.fitSplineForStraightening();
   else if (options != null && options.indexOf("remove") != -1) p.removeSplineFit();
   else p.fitSpline();
   imp.draw();
   LineWidthAdjuster.update();
 }
Exemplo n.º 9
0
 void loadTransformation(String filename, ResultsTable res) {
   try {
     String line;
     FileReader fr = new FileReader(filename);
     BufferedReader br = new BufferedReader(fr);
     if (!br.readLine().equals(" 	Z-Step	Raw Width minus Heigh	Calibration Width minus Height")) {
       IJ.error("File does not seam to be an Astigmatism calibration file");
       return;
     }
     // java.lang.String [] elements = new java.lang.String [3];
     java.lang.String[] elements;
     int counter = 1;
     res.reset();
     while ((line = br.readLine()) != null) {
       IJ.showStatus("Loading element " + counter + "... sit back and relax.");
       counter++;
       line.trim();
       elements = line.split("\t");
       res.incrementCounter();
       res.addValue("Z-Step", Double.parseDouble(elements[1]));
       res.addValue("Raw Width minus Heigh", Double.parseDouble(elements[2]));
       res.addValue("Calibration Width minus Height", Double.parseDouble(elements[3]));
     }
     fr.close();
   } catch (FileNotFoundException e) {
     IJ.error("File not found exception" + e);
     return;
   } catch (IOException e) {
     IJ.error("IOException exception" + e);
     return;
   } catch (NumberFormatException e) {
     IJ.error("Number format exception" + e);
     return;
   }
 }
Exemplo n.º 10
0
  public void run(String arg) {
    ImagePlus imp = WindowManager.getCurrentImage();
    if (imp == null) {
      IJ.noImage();
      return;
    }

    ImageStack stack1 = imp.getStack();
    String fileName = imp.getTitle();
    int endslice = stack1.getSize();

    ImagePlus imp2 = duplicateStack(imp);
    imp2.show();
    String duplicateName = imp2.getTitle();
    // IJ.showMessage("Box",fileName);
    ImageStack stack2 = imp2.getStack();
    stack1.deleteSlice(1);
    stack2.deleteSlice(endslice);

    String calculatorstring =
        ("image1='"
            + fileName
            + "' operation=Subtract image2="
            + imp2.getTitle()
            + " create stack");

    IJ.run("Image Calculator...", calculatorstring);
    ImagePlus imp3 = WindowManager.getCurrentImage();
    imp3.setTitle(fileName + " DeltaF up");
    imp2.getWindow().close();
    imp.getWindow().close();
  }
Exemplo n.º 11
0
 public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) {
   if (doOptions) {
     this.imp = imp;
     this.pfr = pfr;
     GenericDialog gd = new GenericDialog("Binary Options");
     gd.addNumericField("Iterations (1-" + MAX_ITERATIONS + "):", iterations, 0, 3, "");
     gd.addNumericField("Count (1-8):", count, 0, 3, "");
     gd.addCheckbox("Black background", Prefs.blackBackground);
     gd.addCheckbox("Pad edges when eroding", Prefs.padEdges);
     gd.addChoice("EDM output:", outputTypes, outputTypes[EDM.getOutputType()]);
     if (imp != null) {
       gd.addChoice("Do:", operations, operation);
       gd.addPreviewCheckbox(pfr);
       gd.addDialogListener(this);
       previewing = true;
     }
     gd.addHelp(IJ.URL + "/docs/menus/process.html#options");
     gd.showDialog();
     previewing = false;
     if (gd.wasCanceled()) return DONE;
     if (imp == null) { // options dialog only, no do/preview
       dialogItemChanged(gd, null); // read dialog result
       return DONE;
     }
     return operation.equals(NO_OPERATION) ? DONE : IJ.setupDialog(imp, flags);
   } else { // no dialog, 'arg' is operation type
     if (!((ByteProcessor) imp.getProcessor()).isBinary()) {
       IJ.error("8-bit binary (black and white only) image required.");
       return DONE;
     }
     return IJ.setupDialog(imp, flags);
   }
 }
Exemplo n.º 12
0
 public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) {
   this.pfr = pfr;
   String macroOptions = Macro.getOptions();
   if (macroOptions != null) {
     if (macroOptions.indexOf(" interpolate") != -1)
       macroOptions.replaceAll(" interpolate", " interpolation=Bilinear");
     else if (macroOptions.indexOf(" interpolation=") == -1)
       macroOptions = macroOptions + " interpolation=None";
     Macro.setOptions(macroOptions);
   }
   gd = new GenericDialog("Rotate", IJ.getInstance());
   gd.addNumericField("Angle (degrees):", angle, (int) angle == angle ? 1 : 2);
   gd.addNumericField("Grid Lines:", gridLines, 0);
   gd.addChoice("Interpolation:", methods, methods[interpolationMethod]);
   if (bitDepth == 8 || bitDepth == 24)
     gd.addCheckbox("Fill with Background Color", fillWithBackground);
   if (canEnlarge) gd.addCheckbox("Enlarge Image to Fit Result", enlarge);
   else enlarge = false;
   gd.addPreviewCheckbox(pfr);
   gd.addDialogListener(this);
   gd.showDialog();
   drawGridLines(0);
   if (gd.wasCanceled()) {
     return DONE;
   }
   if (!enlarge) flags |= KEEP_PREVIEW; // standard filter without enlarge
   else if (imp.getStackSize() == 1) flags |= NO_CHANGES; // undoable as a "compound filter"
   return IJ.setupDialog(imp, flags);
 }
Exemplo n.º 13
0
 /** Handle menu events. */
 public void actionPerformed(ActionEvent e) {
   if ((e.getSource() instanceof MenuItem)) {
     MenuItem item = (MenuItem) e.getSource();
     String cmd = e.getActionCommand();
     commandName = cmd;
     ImagePlus imp = null;
     if (item.getParent() == Menus.getOpenRecentMenu()) {
       new RecentOpener(cmd); // open image in separate thread
       return;
     } else if (item.getParent() == Menus.getPopupMenu()) {
       Object parent = Menus.getPopupMenu().getParent();
       if (parent instanceof ImageCanvas) imp = ((ImageCanvas) parent).getImage();
     }
     int flags = e.getModifiers();
     hotkey = false;
     actionPerformedTime = System.currentTimeMillis();
     long ellapsedTime = actionPerformedTime - keyPressedTime;
     if (cmd != null && (ellapsedTime >= 200L || !cmd.equals(lastKeyCommand))) {
       if ((flags & Event.ALT_MASK) != 0) IJ.setKeyDown(KeyEvent.VK_ALT);
       if ((flags & Event.SHIFT_MASK) != 0) IJ.setKeyDown(KeyEvent.VK_SHIFT);
       new Executer(cmd, imp);
     }
     lastKeyCommand = null;
     if (IJ.debugMode) IJ.log("actionPerformed: time=" + ellapsedTime + ", " + e);
   }
 }
Exemplo n.º 14
0
 public void reduceHyperstack(ImagePlus imp, int factor, boolean reduceSlices) {
   int channels = imp.getNChannels();
   int slices = imp.getNSlices();
   int frames = imp.getNFrames();
   int zfactor = reduceSlices ? factor : 1;
   int tfactor = reduceSlices ? 1 : factor;
   ImageStack stack = imp.getStack();
   ImageStack stack2 = new ImageStack(imp.getWidth(), imp.getHeight());
   boolean virtual = stack.isVirtual();
   int slices2 = slices / zfactor + ((slices % zfactor) != 0 ? 1 : 0);
   int frames2 = frames / tfactor + ((frames % tfactor) != 0 ? 1 : 0);
   int n = channels * slices2 * frames2;
   int count = 1;
   for (int t = 1; t <= frames; t += tfactor) {
     for (int z = 1; z <= slices; z += zfactor) {
       for (int c = 1; c <= channels; c++) {
         int i = imp.getStackIndex(c, z, t);
         IJ.showProgress(i, n);
         ImageProcessor ip = stack.getProcessor(imp.getStackIndex(c, z, t));
         // IJ.log(count++ +"  "+i+" "+c+" "+z+" "+t);
         stack2.addSlice(stack.getSliceLabel(i), ip);
       }
     }
   }
   imp.setStack(stack2, channels, slices2, frames2);
   Calibration cal = imp.getCalibration();
   if (cal.scaled()) cal.pixelDepth *= zfactor;
   if (virtual) imp.setTitle(imp.getTitle());
   IJ.showProgress(1.0);
 }
Exemplo n.º 15
0
    public synchronized void adjustmentValueChanged(AdjustmentEvent e) {

      if (!checkImage()) {
        IJ.beep();
        IJ.showStatus("No Image");
        return;
      }

      if (e.getSource() == minSlider) {
        adjustMinHue((int) minSlider.getValue());
      } else if (e.getSource() == maxSlider) {
        adjustMaxHue((int) maxSlider.getValue());
      } else if (e.getSource() == minSlider2) {
        adjustMinSat((int) minSlider2.getValue());
      } else if (e.getSource() == maxSlider2) {
        adjustMaxSat((int) maxSlider2.getValue());
      } else if (e.getSource() == minSlider3) {
        adjustMinBri((int) minSlider3.getValue());
      } else if (e.getSource() == maxSlider3) {
        adjustMaxBri((int) maxSlider3.getValue());
      }
      originalB.setEnabled(true);
      updateLabels();
      updatePlot();
      notify();
    }
Exemplo n.º 16
0
  public Channels() {
    super("Channels");
    if (instance != null) {
      instance.toFront();
      return;
    }
    WindowManager.addWindow(this);
    instance = this;
    GridBagLayout gridbag = new GridBagLayout();
    GridBagConstraints c = new GridBagConstraints();
    setLayout(gridbag);
    int y = 0;
    c.gridx = 0;
    c.gridy = y++;
    c.gridwidth = 1;
    c.fill = GridBagConstraints.BOTH;
    c.anchor = GridBagConstraints.CENTER;
    int margin = 32;
    if (IJ.isMacOSX()) margin = 20;
    c.insets = new Insets(10, margin, 10, margin);
    choice = new Choice();
    for (int i = 0; i < modes.length; i++) choice.addItem(modes[i]);
    choice.select(0);
    choice.addItemListener(this);
    add(choice, c);

    CompositeImage ci = getImage();
    int nCheckBoxes = ci != null ? ci.getNChannels() : 3;
    if (nCheckBoxes > CompositeImage.MAX_CHANNELS) nCheckBoxes = CompositeImage.MAX_CHANNELS;
    checkbox = new Checkbox[nCheckBoxes];
    for (int i = 0; i < nCheckBoxes; i++) {
      checkbox[i] = new Checkbox("Channel " + (i + 1), true);
      c.insets = new Insets(0, 25, i < nCheckBoxes - 1 ? 0 : 10, 5);
      c.gridy = y++;
      add(checkbox[i], c);
      checkbox[i].addItemListener(this);
    }

    c.insets = new Insets(0, 15, 10, 15);
    c.fill = GridBagConstraints.NONE;
    c.gridy = y++;
    moreButton = new Button(moreLabel);
    moreButton.addActionListener(this);
    add(moreButton, c);
    update();

    pm = new PopupMenu();
    for (int i = 0; i < menuItems.length; i++) addPopupItem(menuItems[i]);
    add(pm);

    addKeyListener(IJ.getInstance()); // ImageJ handles keyboard shortcuts
    setResizable(false);
    pack();
    if (location == null) {
      GUI.center(this);
      location = getLocation();
    } else setLocation(location);
    show();
  }
Exemplo n.º 17
0
 public void actionPerformed(ActionEvent e) {
   String command = e.getActionCommand();
   if (command == null) return;
   if (command.equals(moreLabel)) {
     Point bloc = moreButton.getLocation();
     pm.show(this, bloc.x, bloc.y);
   } else if (command.equals("Convert to RGB")) IJ.doCommand("Stack to RGB");
   else IJ.doCommand(command);
 }
Exemplo n.º 18
0
 public void dragOver(DropTargetDragEvent e) {
   if (IJ.debugMode) IJ.log("DragOver: " + e.getLocation());
   Point loc = e.getLocation();
   int buttonSize = Toolbar.getButtonSize();
   int width = IJ.getInstance().getSize().width;
   openAsVirtualStack = width - loc.x <= buttonSize;
   if (openAsVirtualStack) IJ.showStatus("<<Open as Virtual Stack>>");
   else IJ.showStatus("<<Drag and Drop>>");
 }
  public void run(String arg) {
    ImageWindow iw = WindowManager.getCurrentWindow();
    pw = jutils.getPW4SelCopy(iw);
    String title = pw.getTitle();
    float[][] yvals = pw.getYValues();
    float[][] xvals = pw.getXValues();
    int length = yvals[0].length;
    if (pw.getShowErrors()) errs = pw.getErrors(0, false);
    int[] colors = pw.getColors();
    colors[0] = 0;
    ScriptEngineManager manager = new ScriptEngineManager();
    engine = manager.getEngineByName("js");
    ce = (Compilable) engine;
    // hitcounter=0;

    c2 = 0.0f;
    iterations = 0;
    checkc2 = false;

    double[] stats = new double[3];
    tempx = new float[length];
    tempdata = new float[length];
    System.arraycopy(xvals[0], 0, tempx, 0, length);
    System.arraycopy(yvals[0], 0, tempdata, 0, length);
    pw.addPoints(tempx, new float[tempx.length], false);
    series = pw.getNpts().length - 1;
    double[] params = new double[10];
    int[] fixes = {0, 0, 0, 1, 1, 1, 1, 1, 1, 1};
    init_options(params, fixes);
    if (!init_functions()) {
      return;
    }

    while (showoptions(params, fixes)) {
      NLLSfit_v2 fitclass;
      if (checkc2) {
        fitclass = new NLLSfit_v2(this, 0);
      } else {
        fitclass = new NLLSfit_v2(this, 0.0001, 50, 0.1);
      }
      float[] fit = fitclass.fitdata(params, fixes, constraints, yvals[0], weights, stats, true);
      pw.updateSeries(fit, series, false);
      c2 = (float) stats[1];
      iterations = (int) stats[0];
    }

    IJ.log("Chi Squared = " + (float) stats[1]);
    IJ.log("Iterations = " + (int) stats[0]);
    for (int i = 0; i < 10; i++) {
      IJ.log("P" + (i + 1) + " = " + (float) params[i] + " fixed = " + fixes[i]);
    }
    IJ.log("AIC = " + (float) stats[2]);
    // IJ.log("hits = "+hitcounter);
    set_options(params, fixes);
  }
Exemplo n.º 20
0
  public boolean beadCalibration3d() {
    imp = IJ.getImage();
    if (imp == null) {
      IJ.noImage();
      return false;
    } else if (imp.getStackSize() == 1) {
      IJ.error("Stack required");
      return false;
    } else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
      // In order to support 32bit images, pict[] must be changed to float[], and  getPixel(x, y);
      // requires a Float.intBitsToFloat() conversion
      IJ.error("8 or 16 bit greyscale image required");
      return false;
    }
    width = imp.getWidth();
    height = imp.getHeight();
    nslices = imp.getStackSize();
    imtitle = imp.getTitle();

    models[0] = "*None*";
    models[1] = "line";
    models[2] = "2nd degree polynomial";
    models[3] = "3rd degree polynomial";
    models[4] = "4th degree polynomial";

    GenericDialog gd = new GenericDialog("3D PALM calibration");
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
    gd.addNumericField(
        "Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
    gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
    gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
    gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
    gd.addCheckbox(
        "Show divergence of bead positions against model",
        prefs.get("QuickPALM.3Dcal_showDivergence", false));
    gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
    gd.addMessage("\n\nDon't forget to save the table in the end...");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.pthrsh", pthrsh * 100);
    cal_z = gd.getNextNumber();
    prefs.set("QuickPALM.z-step", cal_z);
    window = (int) gd.getNextNumber();
    prefs.set("QuickPALM.window", window);
    model = gd.getNextChoice();
    prefs.set("QuickPALM.model", model);
    part_divergence = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
    part_extrainfo = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
    return true;
  }
Exemplo n.º 21
0
  /** Performs actual projection using specified method. */
  public void doProjection() {
    if (imp == null) return;
    sliceCount = 0;
    if (method < AVG_METHOD || method > MEDIAN_METHOD) method = AVG_METHOD;
    for (int slice = startSlice; slice <= stopSlice; slice += increment) sliceCount++;
    if (method == MEDIAN_METHOD) {
      projImage = doMedianProjection();
      return;
    }

    // Create new float processor for projected pixels.
    FloatProcessor fp = new FloatProcessor(imp.getWidth(), imp.getHeight());
    ImageStack stack = imp.getStack();
    RayFunction rayFunc = getRayFunction(method, fp);
    if (IJ.debugMode == true) {
      IJ.log("\nProjecting stack from: " + startSlice + " to: " + stopSlice);
    }

    // Determine type of input image. Explicit determination of
    // processor type is required for subsequent pixel
    // manipulation.  This approach is more efficient than the
    // more general use of ImageProcessor's getPixelValue and
    // putPixel methods.
    int ptype;
    if (stack.getProcessor(1) instanceof ByteProcessor) ptype = BYTE_TYPE;
    else if (stack.getProcessor(1) instanceof ShortProcessor) ptype = SHORT_TYPE;
    else if (stack.getProcessor(1) instanceof FloatProcessor) ptype = FLOAT_TYPE;
    else {
      IJ.error("Z Project", "Non-RGB stack required");
      return;
    }

    // Do the projection.
    for (int n = startSlice; n <= stopSlice; n += increment) {
      IJ.showStatus("ZProjection " + color + ": " + n + "/" + stopSlice);
      IJ.showProgress(n - startSlice, stopSlice - startSlice);
      projectSlice(stack.getPixels(n), rayFunc, ptype);
    }

    // Finish up projection.
    if (method == SUM_METHOD) {
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else if (method == SD_METHOD) {
      rayFunc.postProcess();
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else {
      rayFunc.postProcess();
      projImage = makeOutputImage(imp, fp, ptype);
    }

    if (projImage == null) IJ.error("Z Project", "Error computing projection.");
  }
Exemplo n.º 22
0
  public void run(String arg) {
    int[] wList = WindowManager.getIDList();
    if (wList == null) {
      IJ.error("No images are open.");
      return;
    }

    double thalf = 0.5;
    boolean keep;

    GenericDialog gd = new GenericDialog("Bleach correction");

    gd.addNumericField("t½:", thalf, 1);
    gd.addCheckbox("Keep source stack:", true);
    gd.showDialog();
    if (gd.wasCanceled()) return;

    long start = System.currentTimeMillis();
    thalf = gd.getNextNumber();
    keep = gd.getNextBoolean();
    if (keep) IJ.run("Duplicate...", "title='Bleach corrected' duplicate");
    ImagePlus imp1 = WindowManager.getCurrentImage();
    int d1 = imp1.getStackSize();
    double v1, v2;
    int width = imp1.getWidth();
    int height = imp1.getHeight();
    ImageProcessor ip1, ip2, ip3;

    int slices = imp1.getStackSize();
    ImageStack stack1 = imp1.getStack();
    ImageStack stack2 = imp1.getStack();
    int currentSlice = imp1.getCurrentSlice();

    for (int n = 1; n <= slices; n++) {
      ip1 = stack1.getProcessor(n);
      ip3 = stack1.getProcessor(1);
      ip2 = stack2.getProcessor(n);
      for (int x = 0; x < width; x++) {
        for (int y = 0; y < height; y++) {
          v1 = ip1.getPixelValue(x, y);
          v2 = ip3.getPixelValue(x, y);

          // =B8/(EXP(-C$7*A8))
          v1 = (v1 / Math.exp(-n * thalf));
          ip2.putPixelValue(x, y, v1);
        }
      }
      IJ.showProgress((double) n / slices);
      IJ.showStatus(n + "/" + slices);
    }

    // stack2.show();
    imp1.updateAndDraw();
  }
Exemplo n.º 23
0
 private boolean checkImage() {
   imp = WindowManager.getCurrentImage();
   if (imp == null) {
     IJ.beep();
     IJ.showStatus("No image");
     return false;
   }
   ip = setup(imp);
   if (ip == null) return false;
   return true;
 }
Exemplo n.º 24
0
 private void rotate(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (IJ.macroRunning()) {
     String options = Macro.getOptions();
     if (options != null
         && (options.indexOf("grid=") != -1 || options.indexOf("interpolat") != -1)) {
       IJ.run("Rotate... ", options); // run Image>Transform>Rotate
       return;
     }
   }
   (new RoiRotator()).run("");
 }
Exemplo n.º 25
0
 boolean setProperties(String title, Roi roi) {
   Frame f = WindowManager.getFrontWindow();
   if (f != null && f.getTitle().indexOf("3D Viewer") != -1) return false;
   if (roi == null) {
     IJ.error("This command requires a selection.");
     return false;
   }
   RoiProperties rp = new RoiProperties(title, roi);
   boolean ok = rp.showDialog();
   if (IJ.debugMode) IJ.log(roi.getDebugInfo());
   return ok;
 }
Exemplo n.º 26
0
 void createMaskFromThreshold(ImagePlus imp) {
   ImageProcessor ip = imp.getProcessor();
   if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) {
     IJ.error("Create Mask", "Area selection or thresholded image required");
     return;
   }
   double t1 = ip.getMinThreshold();
   double t2 = ip.getMaxThreshold();
   IJ.run("Duplicate...", "title=mask");
   ImagePlus imp2 = WindowManager.getCurrentImage();
   ImageProcessor ip2 = imp2.getProcessor();
   ip2.setThreshold(t1, t2, ImageProcessor.NO_LUT_UPDATE);
   IJ.run("Convert to Mask");
 }
Exemplo n.º 27
0
 private String displayRanges(ImagePlus imp) {
   LUT[] luts = imp.getLuts();
   if (luts == null) return "";
   String s = "Display ranges\n";
   int n = luts.length;
   if (n > 7) n = 7;
   for (int i = 0; i < n; i++) {
     double min = luts[i].min;
     double max = luts[i].max;
     int digits = (int) min == min && (int) max == max ? 0 : 2;
     s += "  " + (i + 1) + ": " + IJ.d2s(min, digits) + "-" + IJ.d2s(max, digits) + "\n";
   }
   return s;
 }
Exemplo n.º 28
0
 void doIterations(ImageProcessor ip, String mode) {
   if (escapePressed) return;
   if (!previewing && iterations > 1) IJ.showStatus(arg + "... press ESC to cancel");
   for (int i = 0; i < iterations; i++) {
     if (Thread.currentThread().isInterrupted()) return;
     if (IJ.escapePressed()) {
       escapePressed = true;
       ip.reset();
       return;
     }
     if (mode.equals("erode")) ((ByteProcessor) ip).erode(count, background);
     else ((ByteProcessor) ip).dilate(count, background);
   }
 }
Exemplo n.º 29
0
 /** Quit using a separate thread, hopefully avoiding thread deadlocks. */
 public void run() {
   quitting = true;
   boolean changes = false;
   int[] wList = WindowManager.getIDList();
   if (wList != null) {
     for (int i = 0; i < wList.length; i++) {
       ImagePlus imp = WindowManager.getImage(wList[i]);
       if (imp != null && imp.changes == true) {
         changes = true;
         break;
       }
     }
   }
   Frame[] frames = WindowManager.getNonImageWindows();
   if (frames != null) {
     for (int i = 0; i < frames.length; i++) {
       if (frames[i] != null && (frames[i] instanceof Editor)) {
         if (((Editor) frames[i]).fileChanged()) {
           changes = true;
           break;
         }
       }
     }
   }
   if (windowClosed
       && !changes
       && Menus.window.getItemCount() > Menus.WINDOW_MENU_ITEMS
       && !(IJ.macroRunning() && WindowManager.getImageCount() == 0)) {
     GenericDialog gd = new GenericDialog("ImageJ", this);
     gd.addMessage("Are you sure you want to quit ImageJ?");
     gd.showDialog();
     quitting = !gd.wasCanceled();
     windowClosed = false;
   }
   if (!quitting) return;
   if (!WindowManager.closeAllWindows()) {
     quitting = false;
     return;
   }
   // IJ.log("savePreferences");
   if (applet == null) {
     saveWindowLocations();
     Prefs.savePreferences();
   }
   IJ.cleanup();
   // setVisible(false);
   // IJ.log("dispose");
   dispose();
   if (exitWhenQuitting) System.exit(0);
 }
 boolean init_functions() {
   GenericDialog gd = new GenericDialog("Fitting Options");
   gd.addStringField("Extra Definitions", exdef, 50);
   gd.addCheckbox("Weight Using Plot Errors", false);
   gd.addStringField("Weighting Equation (y is for data)", weightfunction, 50);
   gd.addStringField("Fit_Equation", function, 50);
   gd.showDialog();
   if (gd.wasCanceled()) {
     return false;
   }
   exdef = gd.getNextString();
   boolean errweights = gd.getNextBoolean();
   weightfunction = gd.getNextString();
   function = gd.getNextString();
   // first initialize the weights
   weights = new float[tempdata.length];
   if (errweights
       || weightfunction.equals("")
       || weightfunction == null
       || weightfunction == "1.0") {
     if (errweights) {
       for (int i = 0; i < tempdata.length; i++) weights[i] = 1.0f / (errs[i] * errs[i]);
     } else {
       for (int i = 0; i < tempdata.length; i++) weights[i] = 1.0f;
     }
   } else {
     for (int i = 0; i < tempdata.length; i++) {
       String script =
           "y =" + tempdata[i] + "; " + "x =" + tempx[i] + "; " + "retval=" + weightfunction + ";";
       Double temp = new Double(0.0);
       try {
         temp = (Double) engine.eval(script);
       } catch (Exception e) {
         IJ.log(e.getMessage());
       }
       if (!(temp.isInfinite() || temp.isNaN())) {
         weights[i] = temp.floatValue();
       }
     }
   }
   // now compile the function script
   try {
     String script1 = exdef + "; retval=" + function + ";";
     cs = ce.compile(script1);
   } catch (Exception e) {
     IJ.log(e.toString());
     return false;
   }
   return true;
 }