예제 #1
0
  @Test(dataProvider = "RepeatDetectorTest")
  public void testRepeatDetectorTest(RepeatDetectorTest cfg) {

    // test alleles are equal
    Assert.assertEquals(
        VariantContextUtils.isTandemRepeat(cfg.vc, cfg.ref.getBytes()), cfg.isTrueRepeat);
  }
예제 #2
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  @Test(dataProvider = "mergeAlleles")
  public void testMergeAlleles(MergeAllelesTest cfg) {
    final List<VariantContext> inputs = new ArrayList<VariantContext>();

    int i = 0;
    for (final List<Allele> alleles : cfg.inputs) {
      final String name = "vcf" + ++i;
      inputs.add(makeVC(name, alleles));
    }

    final List<String> priority = vcs2priority(inputs);

    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            inputs,
            priority,
            VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
            VariantContextUtils.GenotypeMergeType.PRIORITIZE,
            false,
            false,
            "set",
            false,
            false);

    Assert.assertEquals(merged.getAlleles(), cfg.expected);
  }
예제 #3
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  @Test
  public void testAnnotationSet() {
    for (final boolean annotate : Arrays.asList(true, false)) {
      for (final String set : Arrays.asList("set", "combine", "x")) {
        final List<String> priority = Arrays.asList("1", "2");
        VariantContext vc1 = makeVC("1", Arrays.asList(Aref, T), VariantContext.PASSES_FILTERS);
        VariantContext vc2 = makeVC("2", Arrays.asList(Aref, T), VariantContext.PASSES_FILTERS);

        final VariantContext merged =
            VariantContextUtils.simpleMerge(
                genomeLocParser,
                Arrays.asList(vc1, vc2),
                priority,
                VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
                VariantContextUtils.GenotypeMergeType.PRIORITIZE,
                annotate,
                false,
                set,
                false,
                false);

        if (annotate)
          Assert.assertEquals(merged.getAttribute(set), VariantContextUtils.MERGE_INTERSECTION);
        else Assert.assertFalse(merged.hasAttribute(set));
      }
    }
  }
예제 #4
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  @Test(dataProvider = "mergeFiltered")
  public void testMergeFiltered(MergeFilteredTest cfg) {
    final List<String> priority = vcs2priority(cfg.inputs);
    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            cfg.inputs,
            priority,
            cfg.type,
            VariantContextUtils.GenotypeMergeType.PRIORITIZE,
            true,
            false,
            "set",
            false,
            false);

    // test alleles are equal
    Assert.assertEquals(merged.getAlleles(), cfg.expected.getAlleles());

    // test set field
    Assert.assertEquals(merged.getAttribute("set"), cfg.setExpected);

    // test filter field
    Assert.assertEquals(merged.getFilters(), cfg.expected.getFilters());
  }
예제 #5
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파일: PhasingUtils.java 프로젝트: nh13/gatk
  static boolean mergeIntoMNPvalidationCheck(
      GenomeLocParser genomeLocParser, VariantContext vc1, VariantContext vc2) {
    GenomeLoc loc1 = VariantContextUtils.getLocation(genomeLocParser, vc1);
    GenomeLoc loc2 = VariantContextUtils.getLocation(genomeLocParser, vc2);

    if (!loc1.onSameContig(loc2))
      throw new ReviewedStingException("Can only merge vc1, vc2 if on the same chromosome");

    if (!loc1.isBefore(loc2))
      throw new ReviewedStingException("Can only merge if vc1 is BEFORE vc2");

    if (vc1.isFiltered() || vc2.isFiltered()) return false;

    if (!vc1.getSampleNames()
        .equals(vc2.getSampleNames())) // vc1, vc2 refer to different sample sets
    return false;

    if (!allGenotypesAreUnfilteredAndCalled(vc1) || !allGenotypesAreUnfilteredAndCalled(vc2))
      return false;

    return true;
  }
예제 #6
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  @Test(expectedExceptions = UserException.class)
  public void testMergeGenotypesRequireUnique() {
    final VariantContext vc1 = makeVC("1", Arrays.asList(Aref, T), makeG("s1", Aref, T, -1));
    final VariantContext vc2 = makeVC("2", Arrays.asList(Aref, T), makeG("s1", Aref, T, -2));

    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            Arrays.asList(vc1, vc2),
            null,
            VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
            VariantContextUtils.GenotypeMergeType.REQUIRE_UNIQUE,
            false,
            false,
            "set",
            false,
            false);
  }
예제 #7
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  @Test(dataProvider = "mergeGenotypes")
  public void testMergeGenotypes(MergeGenotypesTest cfg) {
    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            cfg.inputs,
            cfg.priority,
            VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
            VariantContextUtils.GenotypeMergeType.PRIORITIZE,
            true,
            false,
            "set",
            false,
            false);

    // test alleles are equal
    Assert.assertEquals(merged.getAlleles(), cfg.expected.getAlleles());

    // test genotypes
    assertGenotypesAreMostlyEqual(merged.getGenotypes(), cfg.expected.getGenotypes());
  }
예제 #8
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  @Test
  public void testMergeGenotypesUniquify() {
    final VariantContext vc1 = makeVC("1", Arrays.asList(Aref, T), makeG("s1", Aref, T, -1));
    final VariantContext vc2 = makeVC("2", Arrays.asList(Aref, T), makeG("s1", Aref, T, -2));

    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            Arrays.asList(vc1, vc2),
            null,
            VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
            VariantContextUtils.GenotypeMergeType.UNIQUIFY,
            false,
            false,
            "set",
            false,
            false);

    // test genotypes
    Assert.assertEquals(
        merged.getSampleNames(), new HashSet<String>(Arrays.asList("s1.1", "s1.2")));
  }
  /**
   * Method for creating JexlVCMatchExp from input walker arguments names and exps. These two arrays
   * contain the name associated with each JEXL expression. initializeMatchExps will parse each
   * expression and return a list of JexlVCMatchExp, in order, that correspond to the names and
   * exps. These are suitable input to match() below.
   *
   * @param names names
   * @param exps expressions
   * @return list of matches
   */
  public static List<JexlVCMatchExp> initializeMatchExps(String[] names, String[] exps) {
    if (names == null || exps == null)
      throw new ReviewedStingException(
          "BUG: neither names nor exps can be null: names "
              + Arrays.toString(names)
              + " exps="
              + Arrays.toString(exps));

    if (names.length != exps.length)
      throw new UserException(
          "Inconsistent number of provided filter names and expressions: names="
              + Arrays.toString(names)
              + " exps="
              + Arrays.toString(exps));

    Map<String, String> map = new HashMap<String, String>();
    for (int i = 0; i < names.length; i++) {
      map.put(names[i], exps[i]);
    }

    return VariantContextUtils.initializeMatchExps(map);
  }
예제 #10
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  @Test(dataProvider = "simplemergersiddata")
  public void testRSIDMerge(SimpleMergeRSIDTest cfg) {
    VariantContext snpVC1 = makeVC("snpvc1", Arrays.asList(Aref, T));
    final List<VariantContext> inputs = new ArrayList<VariantContext>();

    for (final String id : cfg.inputs) {
      inputs.add(new VariantContextBuilder(snpVC1).id(id).make());
    }

    final VariantContext merged =
        VariantContextUtils.simpleMerge(
            genomeLocParser,
            inputs,
            null,
            VariantContextUtils.FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED,
            VariantContextUtils.GenotypeMergeType.UNSORTED,
            false,
            false,
            "set",
            false,
            false);
    Assert.assertEquals(merged.getID(), cfg.expected);
  }
예제 #11
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  public void writeBeagleOutput(
      VariantContext preferredVC, VariantContext otherVC, boolean isValidationSite, double prior) {
    GenomeLoc currentLoc =
        VariantContextUtils.getLocation(getToolkit().getGenomeLocParser(), preferredVC);
    StringBuffer beagleOut = new StringBuffer();

    String marker = String.format("%s:%d ", currentLoc.getContig(), currentLoc.getStart());
    beagleOut.append(marker);
    if (markers != null)
      markers.append(marker).append("\t").append(Integer.toString(markerCounter++)).append("\t");
    for (Allele allele : preferredVC.getAlleles()) {
      String bglPrintString;
      if (allele.isNoCall() || allele.isNull()) bglPrintString = "-";
      else bglPrintString = allele.getBaseString(); // get rid of * in case of reference allele

      beagleOut.append(String.format("%s ", bglPrintString));
      if (markers != null) markers.append(bglPrintString).append("\t");
    }
    if (markers != null) markers.append("\n");

    GenotypesContext preferredGenotypes = preferredVC.getGenotypes();
    GenotypesContext otherGenotypes = goodSite(otherVC) ? otherVC.getGenotypes() : null;
    for (String sample : samples) {
      boolean isMaleOnChrX = CHECK_IS_MALE_ON_CHR_X && getSample(sample).getGender() == Gender.MALE;

      Genotype genotype;
      boolean isValidation;
      // use sample as key into genotypes structure
      if (preferredGenotypes.containsSample(sample)) {
        genotype = preferredGenotypes.get(sample);
        isValidation = isValidationSite;
      } else if (otherGenotypes != null && otherGenotypes.containsSample(sample)) {
        genotype = otherGenotypes.get(sample);
        isValidation = !isValidationSite;
      } else {
        // there is magically no genotype for this sample.
        throw new StingException(
            "Sample "
                + sample
                + " arose with no genotype in variant or validation VCF. This should never happen.");
      }

      /*
       * Use likelihoods if: is validation, prior is negative; or: is not validation, has genotype key
       */
      double[] log10Likelihoods = null;
      if ((isValidation && prior < 0.0) || genotype.hasLikelihoods()) {
        log10Likelihoods = genotype.getLikelihoods().getAsVector();

        // see if we need to randomly mask out genotype in this position.
        if (GenomeAnalysisEngine.getRandomGenerator().nextDouble() <= insertedNoCallRate) {
          // we are masking out this genotype
          log10Likelihoods =
              isMaleOnChrX ? HAPLOID_FLAT_LOG10_LIKELIHOODS : DIPLOID_FLAT_LOG10_LIKELIHOODS;
        }

        if (isMaleOnChrX) {
          log10Likelihoods[1] = -255; // todo -- warning this is dangerous for multi-allele case
        }
      }
      /** otherwise, use the prior uniformly */
      else if (!isValidation && genotype.isCalled() && !genotype.hasLikelihoods()) {
        // hack to deal with input VCFs with no genotype likelihoods.  Just assume the called
        // genotype
        // is confident.  This is useful for Hapmap and 1KG release VCFs.
        double AA = (1.0 - prior) / 2.0;
        double AB = (1.0 - prior) / 2.0;
        double BB = (1.0 - prior) / 2.0;

        if (genotype.isHomRef()) {
          AA = prior;
        } else if (genotype.isHet()) {
          AB = prior;
        } else if (genotype.isHomVar()) {
          BB = prior;
        }

        log10Likelihoods = MathUtils.toLog10(new double[] {AA, isMaleOnChrX ? 0.0 : AB, BB});
      } else {
        log10Likelihoods =
            isMaleOnChrX ? HAPLOID_FLAT_LOG10_LIKELIHOODS : DIPLOID_FLAT_LOG10_LIKELIHOODS;
      }

      writeSampleLikelihoods(beagleOut, preferredVC, log10Likelihoods);
    }

    beagleWriter.println(beagleOut.toString());
  }
예제 #12
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 @Test(dataProvider = "ReverseClippingPositionTestProvider")
 public void testReverseClippingPositionTestProvider(ReverseClippingPositionTestProvider cfg) {
   int result =
       VariantContextUtils.computeReverseClipping(cfg.alleles, cfg.ref.getBytes(), 0, false);
   Assert.assertEquals(result, cfg.expectedClip);
 }
예제 #13
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파일: PhasingUtils.java 프로젝트: nh13/gatk
  static VariantContext reallyMergeIntoMNP(
      VariantContext vc1, VariantContext vc2, ReferenceSequenceFile referenceFile) {
    int startInter = vc1.getEnd() + 1;
    int endInter = vc2.getStart() - 1;
    byte[] intermediateBases = null;
    if (startInter <= endInter) {
      intermediateBases =
          referenceFile.getSubsequenceAt(vc1.getChr(), startInter, endInter).getBases();
      StringUtil.toUpperCase(intermediateBases);
    }
    MergedAllelesData mergeData =
        new MergedAllelesData(
            intermediateBases, vc1, vc2); // ensures that the reference allele is added

    GenotypesContext mergedGenotypes = GenotypesContext.create();
    for (final Genotype gt1 : vc1.getGenotypes()) {
      Genotype gt2 = vc2.getGenotype(gt1.getSampleName());

      List<Allele> site1Alleles = gt1.getAlleles();
      List<Allele> site2Alleles = gt2.getAlleles();

      List<Allele> mergedAllelesForSample = new LinkedList<Allele>();

      /* NOTE: Since merged alleles are added to mergedAllelesForSample in the SAME order as in the input VC records,
        we preserve phase information (if any) relative to whatever precedes vc1:
      */
      Iterator<Allele> all2It = site2Alleles.iterator();
      for (Allele all1 : site1Alleles) {
        Allele all2 = all2It.next(); // this is OK, since allSamplesAreMergeable()

        Allele mergedAllele = mergeData.ensureMergedAllele(all1, all2);
        mergedAllelesForSample.add(mergedAllele);
      }

      double mergedGQ = Math.max(gt1.getLog10PError(), gt2.getLog10PError());
      Set<String> mergedGtFilters =
          new HashSet<
              String>(); // Since gt1 and gt2 were unfiltered, the Genotype remains unfiltered

      Map<String, Object> mergedGtAttribs = new HashMap<String, Object>();
      PhaseAndQuality phaseQual = calcPhaseForMergedGenotypes(gt1, gt2);
      if (phaseQual.PQ != null) mergedGtAttribs.put(ReadBackedPhasingWalker.PQ_KEY, phaseQual.PQ);

      Genotype mergedGt =
          new Genotype(
              gt1.getSampleName(),
              mergedAllelesForSample,
              mergedGQ,
              mergedGtFilters,
              mergedGtAttribs,
              phaseQual.isPhased);
      mergedGenotypes.add(mergedGt);
    }

    String mergedName = mergeVariantContextNames(vc1.getSource(), vc2.getSource());
    double mergedLog10PError = Math.min(vc1.getLog10PError(), vc2.getLog10PError());
    Set<String> mergedFilters =
        new HashSet<
            String>(); // Since vc1 and vc2 were unfiltered, the merged record remains unfiltered
    Map<String, Object> mergedAttribs = mergeVariantContextAttributes(vc1, vc2);

    // ids
    List<String> mergedIDs = new ArrayList<String>();
    if (vc1.hasID()) mergedIDs.add(vc1.getID());
    if (vc2.hasID()) mergedIDs.add(vc2.getID());
    String mergedID =
        mergedIDs.isEmpty()
            ? VCFConstants.EMPTY_ID_FIELD
            : Utils.join(VCFConstants.ID_FIELD_SEPARATOR, mergedIDs);

    VariantContextBuilder mergedBuilder =
        new VariantContextBuilder(
                mergedName,
                vc1.getChr(),
                vc1.getStart(),
                vc2.getEnd(),
                mergeData.getAllMergedAlleles())
            .id(mergedID)
            .genotypes(mergedGenotypes)
            .log10PError(mergedLog10PError)
            .filters(mergedFilters)
            .attributes(mergedAttribs);
    VariantContextUtils.calculateChromosomeCounts(mergedBuilder, true);
    return mergedBuilder.make();
  }
예제 #14
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  /**
   * Main entry function to calculate genotypes of a given VC with corresponding GL's
   *
   * @param tracker Tracker
   * @param refContext Reference context
   * @param rawContext Raw context
   * @param stratifiedContexts Stratified alignment contexts
   * @param vc Input VC
   * @param model GL calculation model
   * @param inheritAttributesFromInputVC Output VC will contain attributes inherited from input vc
   * @return VC with assigned genotypes
   */
  public VariantCallContext calculateGenotypes(
      final RefMetaDataTracker tracker,
      final ReferenceContext refContext,
      final AlignmentContext rawContext,
      Map<String, AlignmentContext> stratifiedContexts,
      final VariantContext vc,
      final GenotypeLikelihoodsCalculationModel.Model model,
      final boolean inheritAttributesFromInputVC,
      final Map<String, org.broadinstitute.sting.utils.genotyper.PerReadAlleleLikelihoodMap>
          perReadAlleleLikelihoodMap) {

    boolean limitedContext =
        tracker == null || refContext == null || rawContext == null || stratifiedContexts == null;

    // initialize the data for this thread if that hasn't been done yet
    if (afcm.get() == null) {
      afcm.set(AFCalcFactory.createAFCalc(UAC, N, logger));
    }

    // estimate our confidence in a reference call and return
    if (vc.getNSamples() == 0) {
      if (limitedContext) return null;
      return (UAC.OutputMode != OUTPUT_MODE.EMIT_ALL_SITES
          ? estimateReferenceConfidence(vc, stratifiedContexts, getTheta(model), false, 1.0)
          : generateEmptyContext(tracker, refContext, stratifiedContexts, rawContext));
    }

    AFCalcResult AFresult = afcm.get().getLog10PNonRef(vc, getAlleleFrequencyPriors(model));

    // is the most likely frequency conformation AC=0 for all alternate alleles?
    boolean bestGuessIsRef = true;

    // determine which alternate alleles have AF>0
    final List<Allele> myAlleles = new ArrayList<Allele>(vc.getAlleles().size());
    final List<Integer> alleleCountsofMLE = new ArrayList<Integer>(vc.getAlleles().size());
    myAlleles.add(vc.getReference());
    for (int i = 0; i < AFresult.getAllelesUsedInGenotyping().size(); i++) {
      final Allele alternateAllele = AFresult.getAllelesUsedInGenotyping().get(i);
      if (alternateAllele.isReference()) continue;

      // we are non-ref if the probability of being non-ref > the emit confidence.
      // the emit confidence is phred-scaled, say 30 => 10^-3.
      // the posterior AF > 0 is log10: -5 => 10^-5
      // we are non-ref if 10^-5 < 10^-3 => -5 < -3
      final boolean isNonRef =
          AFresult.isPolymorphic(alternateAllele, UAC.STANDARD_CONFIDENCE_FOR_EMITTING / -10.0);

      // if the most likely AC is not 0, then this is a good alternate allele to use
      if (isNonRef) {
        myAlleles.add(alternateAllele);
        alleleCountsofMLE.add(AFresult.getAlleleCountAtMLE(alternateAllele));
        bestGuessIsRef = false;
      }
      // if in GENOTYPE_GIVEN_ALLELES mode, we still want to allow the use of a poor allele
      else if (UAC.GenotypingMode
          == GenotypeLikelihoodsCalculationModel.GENOTYPING_MODE.GENOTYPE_GIVEN_ALLELES) {
        myAlleles.add(alternateAllele);
        alleleCountsofMLE.add(AFresult.getAlleleCountAtMLE(alternateAllele));
      }
    }

    final double PoFGT0 = Math.pow(10, AFresult.getLog10PosteriorOfAFGT0());

    // note the math.abs is necessary because -10 * 0.0 => -0.0 which isn't nice
    final double phredScaledConfidence =
        Math.abs(
            !bestGuessIsRef
                    || UAC.GenotypingMode
                        == GenotypeLikelihoodsCalculationModel.GENOTYPING_MODE
                            .GENOTYPE_GIVEN_ALLELES
                ? -10 * AFresult.getLog10PosteriorOfAFEq0()
                : -10 * AFresult.getLog10PosteriorOfAFGT0());

    // return a null call if we don't pass the confidence cutoff or the most likely allele frequency
    // is zero
    if (UAC.OutputMode != OUTPUT_MODE.EMIT_ALL_SITES
        && !passesEmitThreshold(phredScaledConfidence, bestGuessIsRef)) {
      // technically, at this point our confidence in a reference call isn't accurately estimated
      //  because it didn't take into account samples with no data, so let's get a better estimate
      return limitedContext
          ? null
          : estimateReferenceConfidence(vc, stratifiedContexts, getTheta(model), true, PoFGT0);
    }

    // start constructing the resulting VC
    final GenomeLoc loc = genomeLocParser.createGenomeLoc(vc);
    final VariantContextBuilder builder =
        new VariantContextBuilder(
            "UG_call", loc.getContig(), loc.getStart(), loc.getStop(), myAlleles);
    builder.log10PError(phredScaledConfidence / -10.0);
    if (!passesCallThreshold(phredScaledConfidence)) builder.filters(filter);

    // create the genotypes
    final GenotypesContext genotypes = afcm.get().subsetAlleles(vc, myAlleles, true, ploidy);
    builder.genotypes(genotypes);

    // print out stats if we have a writer
    if (verboseWriter != null && !limitedContext)
      printVerboseData(refContext.getLocus().toString(), vc, PoFGT0, phredScaledConfidence, model);

    // *** note that calculating strand bias involves overwriting data structures, so we do that
    // last
    final HashMap<String, Object> attributes = new HashMap<String, Object>();

    // inherit attributed from input vc if requested
    if (inheritAttributesFromInputVC) attributes.putAll(vc.getAttributes());
    // if the site was downsampled, record that fact
    if (!limitedContext && rawContext.hasPileupBeenDownsampled())
      attributes.put(VCFConstants.DOWNSAMPLED_KEY, true);

    if (UAC.ANNOTATE_NUMBER_OF_ALLELES_DISCOVERED)
      attributes.put(NUMBER_OF_DISCOVERED_ALLELES_KEY, vc.getAlternateAlleles().size());

    // add the MLE AC and AF annotations
    if (alleleCountsofMLE.size() > 0) {
      attributes.put(VCFConstants.MLE_ALLELE_COUNT_KEY, alleleCountsofMLE);
      final int AN = builder.make().getCalledChrCount();
      final ArrayList<Double> MLEfrequencies = new ArrayList<Double>(alleleCountsofMLE.size());
      // the MLEAC is allowed to be larger than the AN (e.g. in the case of all PLs being 0, the GT
      // is ./. but the exact model may arbitrarily choose an AC>1)
      for (int AC : alleleCountsofMLE) MLEfrequencies.add(Math.min(1.0, (double) AC / (double) AN));
      attributes.put(VCFConstants.MLE_ALLELE_FREQUENCY_KEY, MLEfrequencies);
    }

    if (UAC.COMPUTE_SLOD && !limitedContext && !bestGuessIsRef) {
      // final boolean DEBUG_SLOD = false;

      // the overall lod
      // double overallLog10PofNull = AFresult.log10AlleleFrequencyPosteriors[0];
      double overallLog10PofF = AFresult.getLog10LikelihoodOfAFGT0();
      // if ( DEBUG_SLOD ) System.out.println("overallLog10PofF=" + overallLog10PofF);

      List<Allele> allAllelesToUse = builder.make().getAlleles();

      // the forward lod
      VariantContext vcForward =
          calculateLikelihoods(
              tracker,
              refContext,
              stratifiedContexts,
              AlignmentContextUtils.ReadOrientation.FORWARD,
              allAllelesToUse,
              false,
              model,
              perReadAlleleLikelihoodMap);
      AFresult = afcm.get().getLog10PNonRef(vcForward, getAlleleFrequencyPriors(model));
      // double[] normalizedLog10Posteriors =
      // MathUtils.normalizeFromLog10(AFresult.log10AlleleFrequencyPosteriors, true);
      double forwardLog10PofNull = AFresult.getLog10LikelihoodOfAFEq0();
      double forwardLog10PofF = AFresult.getLog10LikelihoodOfAFGT0();
      // if ( DEBUG_SLOD ) System.out.println("forwardLog10PofNull=" + forwardLog10PofNull + ",
      // forwardLog10PofF=" + forwardLog10PofF);

      // the reverse lod
      VariantContext vcReverse =
          calculateLikelihoods(
              tracker,
              refContext,
              stratifiedContexts,
              AlignmentContextUtils.ReadOrientation.REVERSE,
              allAllelesToUse,
              false,
              model,
              perReadAlleleLikelihoodMap);
      AFresult = afcm.get().getLog10PNonRef(vcReverse, getAlleleFrequencyPriors(model));
      // normalizedLog10Posteriors =
      // MathUtils.normalizeFromLog10(AFresult.log10AlleleFrequencyPosteriors, true);
      double reverseLog10PofNull = AFresult.getLog10LikelihoodOfAFEq0();
      double reverseLog10PofF = AFresult.getLog10LikelihoodOfAFGT0();
      // if ( DEBUG_SLOD ) System.out.println("reverseLog10PofNull=" + reverseLog10PofNull + ",
      // reverseLog10PofF=" + reverseLog10PofF);

      double forwardLod = forwardLog10PofF + reverseLog10PofNull - overallLog10PofF;
      double reverseLod = reverseLog10PofF + forwardLog10PofNull - overallLog10PofF;
      // if ( DEBUG_SLOD ) System.out.println("forward lod=" + forwardLod + ", reverse lod=" +
      // reverseLod);

      // strand score is max bias between forward and reverse strands
      double strandScore = Math.max(forwardLod, reverseLod);
      // rescale by a factor of 10
      strandScore *= 10.0;
      // logger.debug(String.format("SLOD=%f", strandScore));

      if (!Double.isNaN(strandScore)) attributes.put("SB", strandScore);
    }

    // finish constructing the resulting VC
    builder.attributes(attributes);
    VariantContext vcCall = builder.make();

    // if we are subsetting alleles (either because there were too many or because some were not
    // polymorphic)
    // then we may need to trim the alleles (because the original VariantContext may have had to pad
    // at the end).
    if (myAlleles.size() != vc.getAlleles().size()
        && !limitedContext) // limitedContext callers need to handle allele trimming on their own to
                            // keep their perReadAlleleLikelihoodMap alleles in sync
    vcCall = VariantContextUtils.reverseTrimAlleles(vcCall);

    if (annotationEngine != null
        && !limitedContext) { // limitedContext callers need to handle annotations on their own by
                              // calling their own annotationEngine
      // Note: we want to use the *unfiltered* and *unBAQed* context for the annotations
      final ReadBackedPileup pileup = rawContext.getBasePileup();
      stratifiedContexts = AlignmentContextUtils.splitContextBySampleName(pileup);

      vcCall =
          annotationEngine.annotateContext(
              tracker, refContext, stratifiedContexts, vcCall, perReadAlleleLikelihoodMap);
    }

    return new VariantCallContext(vcCall, confidentlyCalled(phredScaledConfidence, PoFGT0));
  }