/** * Execute plugin functionality: stack FFT with window function, max projection over all slices * (phase, Z angle), blank out central 1/8 circle (set to min value), display min-max. */ public ResultSet exec(ImagePlus... imps) { ImagePlus imp = imps[0]; Util_StackFFT2D stackFFT2D = new Util_StackFFT2D(); stackFFT2D.resultTypeChoice = Util_StackFFT2D.resultType[1]; ImagePlus impF = stackFFT2D.exec(imp); IJ.run(impF, "Z Project...", "projection=[Max Intensity]"); ImagePlus impProjF = ij.WindowManager.getCurrentImage(); maskCentralRegion(impProjF); if (impProjF.isComposite()) { // display grayscale, not colored composite CompositeImage ci = (CompositeImage) impProjF; ci.setMode(IJ.GRAYSCALE); impProjF.updateAndDraw(); } displayMinToMax(impProjF); impProjF.setTitle(I1l.makeTitle(imps[0], TLA)); String shortInfo = "Maximum intensity projection of log" + " (amplitude^2) 2D FFT stack, central region masked," + " rescaled (min-max) to improve contrast of the relevant" + " frequency range."; results.addImp(shortInfo, impProjF); results.addInfo( "How to interpret", "look for clean 1st & 2nd" + " order spots, similar across angles. Note that spot" + " intensity depends on image content."); return results; }
/** * Copies the LUTs and display mode of 'imp' to this image. Does nothing if 'imp' is not a * CompositeImage or 'imp' and this image do not have the same number of channels. */ public synchronized void copyLuts(ImagePlus imp) { int channels = getNChannels(); if (!imp.isComposite() || imp.getNChannels() != channels) return; CompositeImage ci = (CompositeImage) imp; LUT[] luts = ci.getLuts(); if (luts != null && luts.length == channels) { lut = luts; cip = null; } int mode2 = ci.getMode(); setMode(mode2); if (mode2 == COMPOSITE) { boolean[] active2 = ci.getActiveChannels(); for (int i = 0; i < MAX_CHANNELS; i++) active[i] = active2[i]; } if (ci.hasCustomLuts()) customLuts = true; }
public void reset() { int nChannels = getNChannels(); if (nChannels > MAX_CHANNELS && getMode() == COMPOSITE) setMode(COLOR); setup(nChannels, getImageStack()); }
public void itemStateChanged(ItemEvent e) { ImagePlus imp = WindowManager.getCurrentImage(); if (imp == null) return; if (!imp.isComposite()) { int channels = imp.getNChannels(); if (channels == 1 && imp.getStackSize() <= 4) channels = imp.getStackSize(); if (imp.getBitDepth() == 24 || (channels > 1 && channels < CompositeImage.MAX_CHANNELS)) { GenericDialog gd = new GenericDialog(imp.getTitle()); gd.addMessage("Convert to multi-channel composite image?"); gd.showDialog(); if (gd.wasCanceled()) return; else IJ.doCommand("Make Composite"); } else { IJ.error( "Channels", "A composite image is required (e.g., " + moreLabel + " Open HeLa Cells),\nor create one using " + moreLabel + " Make Composite."); return; } } if (!imp.isComposite()) return; CompositeImage ci = (CompositeImage) imp; Object source = e.getSource(); if (source == choice) { int index = ((Choice) source).getSelectedIndex(); switch (index) { case 0: ci.setMode(IJ.COMPOSITE); break; case 1: ci.setMode(IJ.COLOR); break; case 2: ci.setMode(IJ.GRAYSCALE); break; } ci.updateAndDraw(); if (Recorder.record) { String mode = null; switch (index) { case 0: mode = "composite"; break; case 1: mode = "color"; break; case 2: mode = "grayscale"; break; } Recorder.record("Stack.setDisplayMode", mode); } } else if (source instanceof Checkbox) { for (int i = 0; i < checkbox.length; i++) { Checkbox cb = (Checkbox) source; if (cb == checkbox[i]) { if (ci.getMode() == IJ.COMPOSITE) { boolean[] active = ci.getActiveChannels(); active[i] = cb.getState(); if (Recorder.record) { String str = ""; for (int c = 0; c < ci.getNChannels(); c++) str += active[c] ? "1" : "0"; Recorder.record("Stack.setActiveChannels", str); Recorder.record("//Stack.toggleChannel", imp.getChannel()); } } else { imp.setPosition(i + 1, imp.getSlice(), imp.getFrame()); if (Recorder.record) Recorder.record("Stack.setChannel", i + 1); } ci.updateAndDraw(); return; } } } }