コード例 #1
0
  /** Initialize the stratifications, evaluations, evaluation contexts, and reporting object */
  public void initialize() {
    // Just list the modules, and exit quickly.
    if (LIST) {
      variantEvalUtils.listModulesAndExit();
    }

    // maintain the full list of comps
    comps.addAll(compsProvided);
    if (dbsnp.dbsnp.isBound()) {
      comps.add(dbsnp.dbsnp);
      knowns.add(dbsnp.dbsnp);
    }

    // Add a dummy comp track if none exists
    if (comps.size() == 0)
      comps.add(
          new RodBinding<VariantContext>(VariantContext.class, "none", "UNBOUND", "", new Tags()));

    // Set up set of additional knowns
    for (RodBinding<VariantContext> compRod : comps) {
      if (KNOWN_NAMES.contains(compRod.getName())) knowns.add(compRod);
    }

    // Now that we have all the rods categorized, determine the sample list from the eval rods.
    Map<String, VCFHeader> vcfRods = VCFUtils.getVCFHeadersFromRods(getToolkit(), evals);
    Set<String> vcfSamples =
        SampleUtils.getSampleList(vcfRods, VariantContextUtils.GenotypeMergeType.REQUIRE_UNIQUE);

    // Load the sample list
    sampleNamesForEvaluation.addAll(
        SampleUtils.getSamplesFromCommandLineInput(vcfSamples, SAMPLE_EXPRESSIONS));
    numSamples = NUM_SAMPLES > 0 ? NUM_SAMPLES : sampleNamesForEvaluation.size();

    if (Arrays.asList(STRATIFICATIONS_TO_USE).contains("Sample")) {
      sampleNamesForStratification.addAll(sampleNamesForEvaluation);
    }
    sampleNamesForStratification.add(ALL_SAMPLE_NAME);

    // Initialize select expressions
    for (VariantContextUtils.JexlVCMatchExp jexl :
        VariantContextUtils.initializeMatchExps(SELECT_NAMES, SELECT_EXPS)) {
      SortableJexlVCMatchExp sjexl = new SortableJexlVCMatchExp(jexl.name, jexl.exp);
      jexlExpressions.add(sjexl);
    }

    // Initialize the set of stratifications and evaluations to use
    stratificationObjects =
        variantEvalUtils.initializeStratificationObjects(
            this, NO_STANDARD_STRATIFICATIONS, STRATIFICATIONS_TO_USE);
    Set<Class<? extends VariantEvaluator>> evaluationObjects =
        variantEvalUtils.initializeEvaluationObjects(NO_STANDARD_MODULES, MODULES_TO_USE);
    for (VariantStratifier vs : getStratificationObjects()) {
      if (vs.getName().equals("Filter")) byFilterIsEnabled = true;
      else if (vs.getName().equals("Sample")) perSampleIsEnabled = true;
    }

    if (intervalsFile != null) {
      boolean fail = true;
      for (final VariantStratifier vs : stratificationObjects) {
        if (vs.getClass().equals(IntervalStratification.class)) fail = false;
      }
      if (fail)
        throw new UserException.BadArgumentValue(
            "ST", "stratIntervals argument provided but -ST IntervalStratification not provided");
    }

    // Initialize the evaluation contexts
    evaluationContexts =
        variantEvalUtils.initializeEvaluationContexts(
            stratificationObjects, evaluationObjects, null, null);

    // Initialize report table
    report = variantEvalUtils.initializeGATKReport(stratificationObjects, evaluationObjects);

    // Load ancestral alignments
    if (ancestralAlignmentsFile != null) {
      try {
        ancestralAlignments = new IndexedFastaSequenceFile(ancestralAlignmentsFile);
      } catch (FileNotFoundException e) {
        throw new ReviewedStingException(
            String.format(
                "The ancestral alignments file, '%s', could not be found",
                ancestralAlignmentsFile.getAbsolutePath()));
      }
    }

    // initialize CNVs
    if (knownCNVsFile != null) {
      knownCNVsByContig = createIntervalTreeByContig(knownCNVsFile);
    }
  }
コード例 #2
0
ファイル: SelectVariants.java プロジェクト: singerma/gatk
  /** Set up the VCF writer, the sample expressions and regexs, and the JEXL matcher */
  public void initialize() {
    // Get list of samples to include in the output
    List<String> rodNames = Arrays.asList(variantCollection.variants.getName());

    Map<String, VCFHeader> vcfRods = VCFUtils.getVCFHeadersFromRods(getToolkit(), rodNames);
    TreeSet<String> vcfSamples =
        new TreeSet<String>(
            SampleUtils.getSampleList(
                vcfRods, VariantContextUtils.GenotypeMergeType.REQUIRE_UNIQUE));

    Collection<String> samplesFromFile = SampleUtils.getSamplesFromFiles(sampleFiles);
    Collection<String> samplesFromExpressions =
        SampleUtils.matchSamplesExpressions(vcfSamples, sampleExpressions);

    // first, add any requested samples
    samples.addAll(samplesFromFile);
    samples.addAll(samplesFromExpressions);
    samples.addAll(sampleNames);

    // if none were requested, we want all of them
    if (samples.isEmpty()) {
      samples.addAll(vcfSamples);
      NO_SAMPLES_SPECIFIED = true;
    }

    // now, exclude any requested samples
    Collection<String> XLsamplesFromFile = SampleUtils.getSamplesFromFiles(XLsampleFiles);
    samples.removeAll(XLsamplesFromFile);
    samples.removeAll(XLsampleNames);

    if (samples.size() == 0 && !NO_SAMPLES_SPECIFIED)
      throw new UserException(
          "All samples requested to be included were also requested to be excluded.");

    for (String sample : samples) logger.info("Including sample '" + sample + "'");

    // if user specified types to include, add these, otherwise, add all possible variant context
    // types to list of vc types to include
    if (TYPES_TO_INCLUDE.isEmpty()) {

      for (VariantContext.Type t : VariantContext.Type.values()) selectedTypes.add(t);

    } else {
      for (VariantContext.Type t : TYPES_TO_INCLUDE) selectedTypes.add(t);
    }
    // Initialize VCF header
    Set<VCFHeaderLine> headerLines = VCFUtils.smartMergeHeaders(vcfRods.values(), logger);
    headerLines.add(new VCFHeaderLine("source", "SelectVariants"));

    if (KEEP_ORIGINAL_CHR_COUNTS) {
      headerLines.add(
          new VCFFormatHeaderLine("AC_Orig", 1, VCFHeaderLineType.Integer, "Original AC"));
      headerLines.add(
          new VCFFormatHeaderLine("AF_Orig", 1, VCFHeaderLineType.Float, "Original AF"));
      headerLines.add(
          new VCFFormatHeaderLine("AN_Orig", 1, VCFHeaderLineType.Integer, "Original AN"));
    }
    vcfWriter.writeHeader(new VCFHeader(headerLines, samples));

    for (int i = 0; i < SELECT_EXPRESSIONS.size(); i++) {
      // It's not necessary that the user supply select names for the JEXL expressions, since those
      // expressions will only be needed for omitting records.  Make up the select names here.
      selectNames.add(String.format("select-%d", i));
    }

    jexls = VariantContextUtils.initializeMatchExps(selectNames, SELECT_EXPRESSIONS);

    // Look at the parameters to decide which analysis to perform
    DISCORDANCE_ONLY = discordanceTrack.isBound();
    if (DISCORDANCE_ONLY)
      logger.info(
          "Selecting only variants discordant with the track: " + discordanceTrack.getName());

    CONCORDANCE_ONLY = concordanceTrack.isBound();
    if (CONCORDANCE_ONLY)
      logger.info(
          "Selecting only variants concordant with the track: " + concordanceTrack.getName());

    if (MENDELIAN_VIOLATIONS) {
      if (FAMILY_STRUCTURE_FILE != null) {
        try {
          for (final String line : new XReadLines(FAMILY_STRUCTURE_FILE)) {
            MendelianViolation mv =
                new MendelianViolation(line, MENDELIAN_VIOLATION_QUAL_THRESHOLD);
            if (samples.contains(mv.getSampleChild())
                && samples.contains(mv.getSampleDad())
                && samples.contains(mv.getSampleMom())) mvSet.add(mv);
          }
        } catch (FileNotFoundException e) {
          throw new UserException.CouldNotReadInputFile(FAMILY_STRUCTURE_FILE, e);
        }
        if (outMVFile != null)
          try {
            outMVFileStream = new PrintStream(outMVFile);
          } catch (FileNotFoundException e) {
            throw new UserException.CouldNotCreateOutputFile(
                outMVFile, "Can't open output file", e);
          }
      } else
        mvSet.add(new MendelianViolation(FAMILY_STRUCTURE, MENDELIAN_VIOLATION_QUAL_THRESHOLD));
    } else if (!FAMILY_STRUCTURE.isEmpty()) {
      mvSet.add(new MendelianViolation(FAMILY_STRUCTURE, MENDELIAN_VIOLATION_QUAL_THRESHOLD));
      MENDELIAN_VIOLATIONS = true;
    }

    SELECT_RANDOM_NUMBER = numRandom > 0;
    if (SELECT_RANDOM_NUMBER) {
      logger.info("Selecting " + numRandom + " variants at random from the variant track");
      variantArray = new RandomVariantStructure[numRandom];
    }

    SELECT_RANDOM_FRACTION = fractionRandom > 0;
    if (SELECT_RANDOM_FRACTION)
      logger.info(
          "Selecting approximately "
              + 100.0 * fractionRandom
              + "% of the variants at random from the variant track");
  }