private void doHSRGBProjection(ImagePlus rgbImp) { ImageStack stack = rgbImp.getStack(); ImageStack stack2 = new ImageStack(stack.getWidth(), stack.getHeight()); for (int i = startSlice; i <= stopSlice; i++) stack2.addSlice(null, stack.getProcessor(i)); startSlice = 1; stopSlice = stack2.getSize(); doRGBProjection(stack2); }
void createNewStack(ImagePlus imp, ImageProcessor ip) { int nSlices = imp.getStackSize(); int w = imp.getWidth(), h = imp.getHeight(); ImagePlus imp2 = imp.createImagePlus(); Rectangle r = ip.getRoi(); boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight(); ImageStack stack1 = imp.getStack(); ImageStack stack2 = new ImageStack(newWidth, newHeight); ImageProcessor ip1, ip2; int method = interpolationMethod; if (w == 1 || h == 1) method = ImageProcessor.NONE; for (int i = 1; i <= nSlices; i++) { IJ.showStatus("Scale: " + i + "/" + nSlices); ip1 = stack1.getProcessor(i); String label = stack1.getSliceLabel(i); if (crop) { ip1.setRoi(r); ip1 = ip1.crop(); } ip1.setInterpolationMethod(method); ip2 = ip1.resize(newWidth, newHeight, averageWhenDownsizing); if (ip2 != null) stack2.addSlice(label, ip2); IJ.showProgress(i, nSlices); } imp2.setStack(title, stack2); Calibration cal = imp2.getCalibration(); if (cal.scaled()) { cal.pixelWidth *= 1.0 / xscale; cal.pixelHeight *= 1.0 / yscale; } IJ.showProgress(1.0); int[] dim = imp.getDimensions(); imp2.setDimensions(dim[2], dim[3], dim[4]); if (imp.isComposite()) { imp2 = new CompositeImage(imp2, ((CompositeImage) imp).getMode()); ((CompositeImage) imp2).copyLuts(imp); } if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true); if (newDepth > 0 && newDepth != oldDepth) imp2 = (new Resizer()).zScale(imp2, newDepth, interpolationMethod); if (imp2 != null) { imp2.show(); imp2.changes = true; } }
public void doHyperStackProjection(boolean allTimeFrames) { int start = startSlice; int stop = stopSlice; int firstFrame = 1; int lastFrame = imp.getNFrames(); if (!allTimeFrames) firstFrame = lastFrame = imp.getFrame(); ImageStack stack = new ImageStack(imp.getWidth(), imp.getHeight()); int channels = imp.getNChannels(); int slices = imp.getNSlices(); if (slices == 1) { slices = imp.getNFrames(); firstFrame = lastFrame = 1; } int frames = lastFrame - firstFrame + 1; increment = channels; boolean rgb = imp.getBitDepth() == 24; for (int frame = firstFrame; frame <= lastFrame; frame++) { for (int channel = 1; channel <= channels; channel++) { startSlice = (frame - 1) * channels * slices + (start - 1) * channels + channel; stopSlice = (frame - 1) * channels * slices + (stop - 1) * channels + channel; if (rgb) doHSRGBProjection(imp); else doProjection(); stack.addSlice(null, projImage.getProcessor()); } } projImage = new ImagePlus(makeTitle(), stack); projImage.setDimensions(channels, 1, frames); if (channels > 1) { projImage = new CompositeImage(projImage, 0); ((CompositeImage) projImage).copyLuts(imp); if (method == SUM_METHOD || method == SD_METHOD) ((CompositeImage) projImage).resetDisplayRanges(); } if (frames > 1) projImage.setOpenAsHyperStack(true); Overlay overlay = imp.getOverlay(); if (overlay != null) { startSlice = start; stopSlice = stop; if (imp.getType() == ImagePlus.COLOR_RGB) projImage.setOverlay(projectRGBHyperStackRois(overlay)); else projImage.setOverlay(projectHyperStackRois(overlay)); } IJ.showProgress(1, 1); }
void createNewStack(ImagePlus imp, ImageProcessor ip) { Rectangle r = ip.getRoi(); boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight(); int nSlices = imp.getStackSize(); ImageStack stack1 = imp.getStack(); ImageStack stack2 = new ImageStack(newWidth, newHeight); ImageProcessor ip1, ip2; boolean interp = interpolate; if (imp.getWidth() == 1 || imp.getHeight() == 1) interp = false; for (int i = 1; i <= nSlices; i++) { IJ.showStatus("Scale: " + i + "/" + nSlices); ip1 = stack1.getProcessor(i); String label = stack1.getSliceLabel(i); if (crop) { ip1.setRoi(r); ip1 = ip1.crop(); } ip1.setInterpolate(interp); ip2 = ip1.resize(newWidth, newHeight); if (ip2 != null) stack2.addSlice(label, ip2); IJ.showProgress(i, nSlices); } ImagePlus imp2 = imp.createImagePlus(); imp2.setStack(title, stack2); Calibration cal = imp2.getCalibration(); if (cal.scaled()) { cal.pixelWidth *= 1.0 / xscale; cal.pixelHeight *= 1.0 / yscale; } int[] dim = imp.getDimensions(); imp2.setDimensions(dim[2], dim[3], dim[4]); IJ.showProgress(1.0); if (imp.isComposite()) { imp2 = new CompositeImage(imp2, 0); ((CompositeImage) imp2).copyLuts(imp); } if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true); imp2.show(); imp2.changes = true; }