コード例 #1
0
  /** This method test the creation and reading behaviour of a MascotEnzymeReader instance. */
  public void testCreationAndReading() {
    final String inputFile = TestCaseLM.getFullFilePath("enzymes_test.txt").replace("%20", " ");
    final Vector control = new Vector(5);
    control.add("Trypsin");
    control.add("Trypsin/P");
    control.add("TrypChymo");
    control.add("PepsinA");
    control.add("None");
    control.add("dualTrypCathep");
    control.add("DuAlTrypCathep2");
    control.add("ReGeXTrypCathep");
    control.add("regexTrypCathep2");

    // With file.
    try {
      MascotEnzymeReader mer = new MascotEnzymeReader(inputFile);
      String[] names = mer.getEnzymeNames();

      // First see if the arrays are equal size.
      Assert.assertEquals(control.size(), names.length);

      Vector controlCopy = (Vector) control.clone();
      // Now check whether we can find all entries.
      for (int i = 0; i < names.length; i++) {
        for (int j = 0; j < controlCopy.size(); j++) {
          if (names[i].equals(controlCopy.get(j))) {
            controlCopy.remove(j);
            break;
          }
        }
      }
      // The control Vector should now be empty.
      Assert.assertEquals(0, controlCopy.size());

      // Next test each separate Enzyme entry.
      Enzyme e = mer.getEnzyme("Trypsin");
      Assert.assertEquals("Trypsin", e.getTitle());
      Assert.assertEquals("KR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("Trypsin/P");
      Assert.assertEquals("Trypsin/P", e.getTitle());
      Assert.assertEquals("KR", new String(e.getCleavage()));
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("TrypChymo");
      Assert.assertEquals("TrypChymo", e.getTitle());
      Assert.assertEquals("FYWLKR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("PepsinA");
      Assert.assertEquals("PepsinA", e.getTitle());
      Assert.assertEquals("FL", new String(e.getCleavage()));
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("None");
      Assert.assertEquals("None", e.getTitle());
      Assert.assertEquals("NONE", new String(e.getCleavage()));
      Assert.assertEquals("NONE", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("dualTrypCathep");
      Assert.assertEquals("dualTrypCathep", e.getTitle());
      Assert.assertEquals("DXR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof DualEnzyme);
      if (e instanceof DualEnzyme) {
        DualEnzyme dual = (DualEnzyme) e;
        Assert.assertEquals("D", new String(dual.getCleavage(DualEnzyme.NTERMINAL)));
        Assert.assertEquals("R", new String(dual.getCleavage(DualEnzyme.CTERMINAL)));
      }
      e = mer.getEnzyme("DuAlTrypCathep2");
      Assert.assertEquals("DuAlTrypCathep2", e.getTitle());
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof DualEnzyme);
      if (e instanceof DualEnzyme) {
        DualEnzyme dual = (DualEnzyme) e;
        Assert.assertEquals("DK", new String(dual.getCleavage(DualEnzyme.NTERMINAL)));
        Assert.assertEquals("RW", new String(dual.getCleavage(DualEnzyme.CTERMINAL)));
      }

      e = mer.getEnzyme("ReGeXTrypCathep");
      Assert.assertEquals("ReGeXTrypCathep", e.getTitle());
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof RegExEnzyme);
      Assert.assertTrue(e.getClass().getName().equals("com.compomics.util.protein.RegExEnzyme"));
      RegExEnzyme regex = (RegExEnzyme) e;
      Assert.assertEquals("[KR]", new String(regex.getCleavage()));

      e = mer.getEnzyme("regexTrypCathep2");
      Assert.assertEquals("regexTrypCathep2", e.getTitle());
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof RegExEnzyme);
      Assert.assertTrue(e.getClass().getName().equals("com.compomics.util.protein.RegExEnzyme"));
      regex = (RegExEnzyme) e;
      Assert.assertEquals("P+R", new String(regex.getCleavage()));
    } catch (IOException ioe) {
      fail("IOException while testing MascotEnzymeReader creation: '" + ioe.getMessage() + "'.");
    }
    // With inputstream.
    try {
      MascotEnzymeReader mer = new MascotEnzymeReader(new FileInputStream(inputFile));
      String[] names = mer.getEnzymeNames();

      // First see if the arrays are equal size.
      Assert.assertEquals(control.size(), names.length);

      Vector controlCopy = (Vector) control.clone();
      // Now check whether we can find all entries.
      for (int i = 0; i < names.length; i++) {
        for (int j = 0; j < controlCopy.size(); j++) {
          if (names[i].equals(controlCopy.get(j))) {
            controlCopy.remove(j);
            break;
          }
        }
      }
      // The control Vector should now be empty.
      Assert.assertEquals(0, controlCopy.size());

      // Next test each separate Enzyme entry.
      Enzyme e = mer.getEnzyme("Trypsin");
      Assert.assertEquals("Trypsin", e.getTitle());
      Assert.assertEquals("KR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("Trypsin/P");
      Assert.assertEquals("Trypsin/P", e.getTitle());
      Assert.assertEquals("KR", new String(e.getCleavage()));
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("TrypChymo");
      Assert.assertEquals("TrypChymo", e.getTitle());
      Assert.assertEquals("FYWLKR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("PepsinA");
      Assert.assertEquals("PepsinA", e.getTitle());
      Assert.assertEquals("FL", new String(e.getCleavage()));
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("None");
      Assert.assertEquals("None", e.getTitle());
      Assert.assertEquals("NONE", new String(e.getCleavage()));
      Assert.assertEquals("NONE", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());

      e = mer.getEnzyme("dualTrypCathep");
      Assert.assertEquals("dualTrypCathep", e.getTitle());
      Assert.assertEquals("DXR", new String(e.getCleavage()));
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof DualEnzyme);
      if (e instanceof DualEnzyme) {
        DualEnzyme dual = (DualEnzyme) e;
        Assert.assertEquals("D", new String(dual.getCleavage(DualEnzyme.NTERMINAL)));
        Assert.assertEquals("R", new String(dual.getCleavage(DualEnzyme.CTERMINAL)));
      }
      e = mer.getEnzyme("DuAlTrypCathep2");
      Assert.assertEquals("DuAlTrypCathep2", e.getTitle());
      // Assert.assertEquals("", new String(e.getCleavage()));
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof DualEnzyme);
      if (e instanceof DualEnzyme) {
        DualEnzyme dual = (DualEnzyme) e;
        Assert.assertEquals("DK", new String(dual.getCleavage(DualEnzyme.NTERMINAL)));
        Assert.assertEquals("RW", new String(dual.getCleavage(DualEnzyme.CTERMINAL)));
      }

      e = mer.getEnzyme("ReGeXTrypCathep");
      Assert.assertEquals("ReGeXTrypCathep", e.getTitle());
      Assert.assertEquals("P", new String(e.getRestrict()));
      Assert.assertEquals(Enzyme.CTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof RegExEnzyme);
      Assert.assertTrue(e.getClass().getName().equals("com.compomics.util.protein.RegExEnzyme"));
      RegExEnzyme regex = (RegExEnzyme) e;
      Assert.assertEquals("[KR]", new String(regex.getCleavage()));

      e = mer.getEnzyme("regexTrypCathep2");
      Assert.assertEquals("regexTrypCathep2", e.getTitle());
      Assert.assertTrue(e.getRestrict() == null);
      Assert.assertEquals(Enzyme.NTERM, e.getPosition());
      Assert.assertEquals(1, e.getMiscleavages());
      Assert.assertTrue(e instanceof RegExEnzyme);
      Assert.assertTrue(e.getClass().getName().equals("com.compomics.util.protein.RegExEnzyme"));
      regex = (RegExEnzyme) e;
      Assert.assertEquals("P+R", new String(regex.getCleavage()));

    } catch (IOException ioe) {
      fail("IOException while testing MascotEnzymeReader creation: '" + ioe.getMessage() + "'.");
    }

    try {
      MascotEnzymeReader mer = new MascotEnzymeReader("ThisFileIsNowhereInTHeFileSystem.grbl");
      fail(
          "MascotEnzymeReader constructor did not respond with an IOException when confrontend with fictious file 'ThisFileIsNowhereInTHeFileSystem.grbl'!");
    } catch (IOException ioe) {
      // Correct throwing of the IOException.
    }
  }
コード例 #2
0
  /**
   * This method test the 'return copy of enzyme' functionality when requesting an enzyme instance.
   */
  public void testCopyOfEnzyme() {
    final String inputFile = TestCaseLM.getFullFilePath("enzymes_test.txt").replace("%20", " ");
    // Try it for a regular Enzyme.
    try {
      MascotEnzymeReader mer = new MascotEnzymeReader(inputFile);
      Enzyme mod = mer.getEnzyme(mer.getEnzymeNames()[0]);

      int mc = mod.getMiscleavages();
      String cleave = new String(mod.getCleavage());

      mod.setMiscleavages(mc + 1);
      mod.setCleavage(cleave + "H");

      // Make sure 'mod' has changed.
      Assert.assertEquals(mc + 1, mod.getMiscleavages());
      Assert.assertEquals(cleave + "H", new String(mod.getCleavage()));

      // Now get another copy of the same enzyme, and see if it has retained the original values.
      Enzyme original = mer.getEnzyme(mer.getEnzymeNames()[0]);
      Assert.assertEquals(mc, original.getMiscleavages());
      Assert.assertEquals(cleave, new String(original.getCleavage()));
    } catch (IOException ioe) {
      fail(
          "IOException while testing whether a received Enzyme is in fact a copy: '"
              + ioe.getMessage()
              + "'.");
    }
    // Try it for a DualEnzyme.
    try {
      MascotEnzymeReader mer = new MascotEnzymeReader(inputFile);
      DualEnzyme mod = (DualEnzyme) mer.getEnzyme("dualTrypCathep");

      int mc = mod.getMiscleavages();
      String ntermCleave = new String(mod.getCleavage(DualEnzyme.NTERMINAL));
      String ctermCleave = new String(mod.getCleavage(DualEnzyme.CTERMINAL));

      mod.setMiscleavages(mc + 1);
      mod.setCleavage(ntermCleave + "H", DualEnzyme.NTERMINAL);
      mod.setCleavage(ctermCleave + "W", DualEnzyme.CTERMINAL);

      // Make sure 'mod' has changed.
      Assert.assertEquals(mc + 1, mod.getMiscleavages());
      Assert.assertEquals(ntermCleave + "H", new String(mod.getCleavage(DualEnzyme.NTERMINAL)));
      Assert.assertEquals(ctermCleave + "W", new String(mod.getCleavage(DualEnzyme.CTERMINAL)));

      // Now get another copy of the same enzyme, and see if it has retained the original values.
      DualEnzyme original = (DualEnzyme) mer.getEnzyme("dualTrypCathep");
      Assert.assertEquals(mc, original.getMiscleavages());
      Assert.assertEquals(ntermCleave, new String(original.getCleavage(DualEnzyme.NTERMINAL)));
      Assert.assertEquals(ctermCleave, new String(original.getCleavage(DualEnzyme.CTERMINAL)));
    } catch (IOException ioe) {
      fail(
          "IOException while testing whether a received Enzyme is in fact a copy: '"
              + ioe.getMessage()
              + "'.");
    }
    // Try it for a RegExEnzyme.
    try {
      MascotEnzymeReader mer = new MascotEnzymeReader(inputFile);
      RegExEnzyme mod = (RegExEnzyme) mer.getEnzyme("regexTrypCathep2");

      int mc = mod.getMiscleavages();
      String cleave = new String(mod.getCleavage());

      mod.setMiscleavages(mc + 1);
      mod.setCleavage(cleave + "H");

      // Make sure 'mod' has changed.
      Assert.assertEquals(mc + 1, mod.getMiscleavages());
      Assert.assertEquals(cleave + "H", new String(mod.getCleavage()));

      // Now get another copy of the same enzyme, and see if it has retained the original values.
      Enzyme original = mer.getEnzyme("regexTrypCathep2");
      Assert.assertEquals(mc, original.getMiscleavages());
      Assert.assertEquals(cleave, new String(original.getCleavage()));
    } catch (IOException ioe) {
      fail(
          "IOException while testing whether a received Enzyme is in fact a copy: '"
              + ioe.getMessage()
              + "'.");
    }
  }