public void run(ImageProcessor ip) { dimz = stack.getSize(); dimy = stack.getWidth(); dimx = stack.getHeight(); SaveDialog sd = new SaveDialog("Save Measurements as Text...", "res", ".dat"); String name = sd.getFileName(); if (name == null) return; String directory = sd.getDirectory(); nb = calculnb(stack); // -1; IJ.showStatus("Measure parameters for the " + nb + " objects ..."); if (nb < 1) { IJ.showMessage("volume must be labeled"); } else { double[] volume_m = new double[nb]; int[] volume_p = new int[nb]; double[] surface = new double[nb]; double[] surfacenb = new double[nb]; double[][][] I = new double[3][3][nb]; double[][] J = new double[3][nb]; double[][][] dir = new double[3][3][nb]; double[] xg = new double[nb]; double[] yg = new double[nb]; double[] zg = new double[nb]; byte[] bord = new byte[nb]; // double[] a = new double[nb]; // double[] b = new double[nb]; // double[] c = new double[nb]; // double[] Fab = new double[nb]; // double[] Fac = new double[nb]; // double[] Fbc = new double[nb]; // double[] sp = new double[nb]; double[][] lmin = new double[nb][3]; double[][] lmax = new double[nb][3]; IJ.showStatus("Measure surfaces ..."); calculmarchsurfstack(stack, nb, surface, volume_m); calculmarchsurfstacknb(stack, nb, surfacenb); // calculvolumestack(stack,nb,volume_p); IJ.showStatus("Measure volumes and inertia ..."); calculcgstack(stack, nb, volume_p, xg, yg, zg); calculinertiestack(stack, nb, xg, yg, zg, I); inertie(nb, I, J, dir); IJ.showStatus("Measure bounding boxes ..."); boitestack(stack, nb, xg, yg, zg, dir, lmin, lmax); borderstack(stack, nb, bord); IJ.showStatus("Save results ..."); sauvegarde( volume_p, volume_m, surface, surfacenb, xg, yg, zg, J, dir, nb, bord, lmin, lmax, directory, name); volume_m = null; volume_p = null; surface = null; xg = null; yg = null; zg = null; } }
void borderstack(final ImageStack stack1, int nb, byte[] b) { final AtomicInteger ai = new AtomicInteger(0); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final byte[][] bord = new byte[dimZ][nb]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi < dimZ; superi = ai.getAndIncrement()) { int k = superi; ImageProcessor ip = stack1.getProcessor(k + 1); for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int val = (int) (ip.getPixelValue(i, j)); if ((val != 0) && (i == 0 || j == 0 || k == 0 || i == dimX - 1 || j == dimY - 1 || k == dimZ - 1)) // bord[k][val-2]=1; bord[k][val - 1] = 1; } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { b[j] *= (1 - bord[i][j]); } } for (int j = 0; j < nb; j++) { b[j] = (byte) (1 - b[j]); } }
public int calculnb(final ImageStack stack1) { final AtomicInteger ai = new AtomicInteger(1); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final int[] value = new int[dimZ]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi <= dimZ; superi = ai.getAndIncrement()) { int current = superi; ImageProcessor ip = stack1.getProcessor(current); for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int val = (int) (ip.getPixelValue(i, j)); if (val > value[current - 1]) value[current - 1] = val; } } } } }; } startAndJoin(threads); int val = 0; for (int i = 0; i < dimZ; i++) { if (val < value[i]) val = value[i]; } return val; }
void calculvolumestack(final ImageStack stack1, int nb, int v[]) { final AtomicInteger ai = new AtomicInteger(1); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final int[][] vol = new int[dimZ][nb]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi <= dimZ; superi = ai.getAndIncrement()) { int current = superi; ImageProcessor ip = stack1.getProcessor(current); for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int val = (int) (ip.getPixelValue(i, j)); // if (val!=0) vol[current-1][val-2]++; if (val != 0) vol[current - 1][val - 1]++; } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { v[j] += vol[i][j]; } } }
void boitestack( final ImageStack stack1, final int nb, final double[] xg, final double[] yg, final double[] zg, final double[][][] dir, double[][] lmin, double[][] lmax) { final AtomicInteger ai = new AtomicInteger(0); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final double[][][] lmint = new double[dimZ][nb][3]; final double[][][] lmaxt = new double[dimZ][nb][3]; for (int k = 0; k < nb; k++) { lmin[k][0] = 100000; lmin[k][1] = 100000; lmin[k][2] = 100000; lmax[k][0] = -100000; lmax[k][1] = -100000; lmax[k][2] = -100000; } for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi < dimZ; superi = ai.getAndIncrement()) { int k = superi; ImageProcessor ip = stack1.getProcessor(k + 1); for (int l = 0; l < nb; l++) { lmint[k][l][0] = 100000; lmint[k][l][1] = 100000; lmint[k][l][2] = 100000; lmaxt[k][l][0] = -100000; lmaxt[k][l][1] = -100000; lmaxt[k][l][2] = -100000; } for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int pix = (int) (ip.getPixelValue(i, j)); if (pix != 0) { // double v1 = // (i-xg[pix-2])*dir[0][0][pix-2]+(j-yg[pix-2])*dir[1][0][pix-2]+(k-zg[pix-2])*dir[2][0][pix-2]; // double v2 = // (i-xg[pix-2])*dir[0][1][pix-2]+(j-yg[pix-2])*dir[1][1][pix-2]+(k-zg[pix-2])*dir[2][1][pix-2]; // double v3 = // (i-xg[pix-2])*dir[0][2][pix-2]+(j-yg[pix-2])*dir[1][2][pix-2]+(k-zg[pix-2])*dir[2][2][pix-2]; // if (v1<lmint[k][pix-2][0]) lmint[k][pix-2][0]=v1; // if (v1>lmaxt[k][pix-2][0]) lmaxt[k][pix-2][0]=v1; // if (v2<lmint[k][pix-2][1]) lmint[k][pix-2][1]=v2; // if (v2>lmaxt[k][pix-2][1]) lmaxt[k][pix-2][1]=v2; // if (v3<lmint[k][pix-2][2]) lmint[k][pix-2][2]=v3; // if (v3>lmaxt[k][pix-2][2]) lmaxt[k][pix-2][2]=v3; double v1 = (i - xg[pix - 1]) * dir[0][0][pix - 1] + (j - yg[pix - 1]) * dir[1][0][pix - 1] + (k - zg[pix - 1]) * dir[2][0][pix - 1]; double v2 = (i - xg[pix - 1]) * dir[0][1][pix - 1] + (j - yg[pix - 1]) * dir[1][1][pix - 1] + (k - zg[pix - 1]) * dir[2][1][pix - 1]; double v3 = (i - xg[pix - 1]) * dir[0][2][pix - 1] + (j - yg[pix - 1]) * dir[1][2][pix - 1] + (k - zg[pix - 1]) * dir[2][2][pix - 1]; if (v1 < lmint[k][pix - 1][0]) lmint[k][pix - 1][0] = v1; if (v1 > lmaxt[k][pix - 1][0]) lmaxt[k][pix - 1][0] = v1; if (v2 < lmint[k][pix - 1][1]) lmint[k][pix - 1][1] = v2; if (v2 > lmaxt[k][pix - 1][1]) lmaxt[k][pix - 1][1] = v2; if (v3 < lmint[k][pix - 1][2]) lmint[k][pix - 1][2] = v3; if (v3 > lmaxt[k][pix - 1][2]) lmaxt[k][pix - 1][2] = v3; } } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { for (int l = 0; l < 3; l++) { if (lmint[i][j][l] < lmin[j][l]) lmin[j][l] = lmint[i][j][l]; if (lmaxt[i][j][l] > lmax[j][l]) lmax[j][l] = lmaxt[i][j][l]; } } lmint[i] = null; lmaxt[i] = null; } }
void calculinertiestack( final ImageStack stack1, int nb, final double[] xg, final double[] yg, final double[] zg, double[][][] I) { final AtomicInteger ai = new AtomicInteger(0); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final double[][][][] inert = new double[dimZ][nb][3][3]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi < dimZ; superi = ai.getAndIncrement()) { int k = superi; ImageProcessor ip = stack1.getProcessor(k + 1); for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int pix = (int) (ip.getPixelValue(i, j)); if (pix != 0) { // // inert[k][pix-2][0][0]+=(i-yg[pix-2])*(i-yg[pix-2])+(k-zg[pix-2])*(k-zg[pix-2])+1.0/6.0; // inert[k][pix-2][0][1]-=(j-xg[pix-2])*(i-yg[pix-2]); // inert[k][pix-2][0][2]-=(j-xg[pix-2])*(k-zg[pix-2]); // // inert[k][pix-2][1][1]+=(j-xg[pix-2])*(j-xg[pix-2])+(k-zg[pix-2])*(k-zg[pix-2])+1.0/6.0; // inert[k][pix-2][1][2]-=(i-yg[pix-2])*(k-zg[pix-2]); // // inert[k][pix-2][2][2]+=(j-xg[pix-2])*(j-xg[pix-2])+(i-yg[pix-2])*(i-yg[pix-2])+1.0/6.0; inert[k][pix - 1][0][0] += (j - yg[pix - 1]) * (j - yg[pix - 1]) + (k - zg[pix - 1]) * (k - zg[pix - 1]) + 1.0 / 6.0; inert[k][pix - 1][0][1] -= (i - xg[pix - 1]) * (j - yg[pix - 1]); inert[k][pix - 1][0][2] -= (i - xg[pix - 1]) * (k - zg[pix - 1]); inert[k][pix - 1][1][1] += (i - xg[pix - 1]) * (i - xg[pix - 1]) + (k - zg[pix - 1]) * (k - zg[pix - 1]) + 1.0 / 6.0; inert[k][pix - 1][1][2] -= (j - yg[pix - 1]) * (k - zg[pix - 1]); inert[k][pix - 1][2][2] += (i - xg[pix - 1]) * (i - xg[pix - 1]) + (j - yg[pix - 1]) * (j - yg[pix - 1]) + 1.0 / 6.0; } } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { for (int l = 0; l < 3; l++) { for (int m = 0; m <= l; m++) { I[l][m][j] += inert[i][j][l][m]; } } } inert[i] = null; } for (int j = 0; j < nb; j++) { I[1][0][j] = I[0][1][j]; I[2][0][j] = I[0][2][j]; I[2][1][j] = I[1][2][j]; } }
void calculcgstack( final ImageStack stack1, int nb, final int[] v, final double[] xg, final double[] yg, final double[] zg) { final AtomicInteger ai = new AtomicInteger(0); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final int[][] vol = new int[dimZ][nb]; final int[][] tmpxg = new int[dimZ][nb]; final int[][] tmpyg = new int[dimZ][nb]; final int[][] tmpzg = new int[dimZ][nb]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi < dimZ; superi = ai.getAndIncrement()) { int k = superi; ImageProcessor ip = stack1.getProcessor(k + 1); for (int j = 0; j < dimY; j++) { for (int i = 0; i < dimX; i++) { int pix = (int) (ip.getPixelValue(i, j)); if (pix != 0) { // tmpxg[k][pix-2] += j; // tmpyg[k][pix-2] += i; // tmpzg[k][pix-2] += k; // vol[k][pix-2]++; tmpxg[k][pix - 1] += i; tmpyg[k][pix - 1] += j; tmpzg[k][pix - 1] += k; vol[k][pix - 1]++; } } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { v[j] += vol[i][j]; xg[j] += tmpxg[i][j]; yg[j] += tmpyg[i][j]; zg[j] += tmpzg[i][j]; } } for (int i = 0; i < nb; i++) { xg[i] = (1.0 * xg[i] / v[i]); yg[i] = (1.0 * yg[i] / v[i]); zg[i] = (1.0 * zg[i] / v[i]); } }
void calculsurfacemarch3stack(final ImageStack stack1, int nb, final double[] tri, double[] s2) { final AtomicInteger ai = new AtomicInteger(0); final int dimX = stack1.getWidth(); final int dimY = stack1.getHeight(); final int dimZ = stack1.getSize(); final double[][] surf = new double[dimZ][nb]; for (int ithread = 0; ithread < threads.length; ithread++) { // Concurrently run in as many threads as CPUs threads[ithread] = new Thread() { public void run() { for (int superi = ai.getAndIncrement(); superi < dimZ - 1; superi = ai.getAndIncrement()) { int k = superi; ImageProcessor ip1, ip2; ip1 = stack1.getProcessor(k + 1); ip2 = stack1.getProcessor(k + 2); for (int j = 0; j < dimY - 1; j++) { for (int i = 0; i < dimX - 1; i++) { int[] p = new int[8]; int p1, p2, p3, p4, p5, p6, p7, p8, ptot; int[] nontab = new int[8]; ptot = (short) 0; p[0] = (int) ip1.getPixelValue(i, j); p[1] = (int) ip1.getPixelValue(i, j + 1); p[2] = (int) ip2.getPixelValue(i, j); p[3] = (int) ip2.getPixelValue(i, j + 1); p[4] = (int) ip1.getPixelValue(i + 1, j); p[5] = (int) ip1.getPixelValue(i + 1, j + 1); p[6] = (int) ip2.getPixelValue(i + 1, j); p[7] = (int) ip2.getPixelValue(i + 1, j + 1); int pixcoul = 0; for (int l = 0; l < 8; l++) nontab[l] = 0; int cpt = 0; // look for different colors for (int l = 0; l < 8; l++) { if (p[l] != 0 && appart(p[l], nontab, 8) == 1) { nontab[cpt] = p[l]; cpt++; } } for (int mm = 0; mm < cpt; mm++) { p1 = 0; p2 = 0; p3 = 0; p4 = 0; p5 = 0; p6 = 0; p7 = 0; p8 = 0; if (p[0] != 0 && p[0] == nontab[mm]) { pixcoul = nontab[mm]; p1 = 1; } if (p[1] != 0 && p[1] == nontab[mm]) { pixcoul = nontab[mm]; p2 = 4; } if (p[2] != 0 && p[2] == nontab[mm]) { pixcoul = nontab[mm]; p3 = 2; } if (p[3] != 0 && p[3] == nontab[mm]) { pixcoul = nontab[mm]; p4 = 8; } if (p[4] != 0 && p[4] == nontab[mm]) { pixcoul = nontab[mm]; p5 = 16; } if (p[5] != 0 && p[5] == nontab[mm]) { pixcoul = nontab[mm]; p6 = 64; } if (p[6] != 0 && p[6] == nontab[mm]) { pixcoul = nontab[mm]; p7 = 32; } if (p[7] != 0 && p[7] == nontab[mm]) { pixcoul = nontab[mm]; p8 = 128; } ptot = (p1 + p2 + p3 + p4 + p5 + p6 + p7 + p8); if (pixcoul != 0) { surf[k][(int) (pixcoul - 1)] += tri[(int) (ptot)]; // surf[k][(int)(pixcoul-2)]+=tri[(int)(ptot)]; } } } } } } }; } startAndJoin(threads); for (int i = 0; i < dimZ; i++) { for (int j = 0; j < nb; j++) { s2[j] += surf[i][j]; } } }