public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) {
this.pfr = pfr;
String macroOptions = Macro.getOptions();
if (macroOptions != null) {
if (macroOptions.indexOf(" interpolate") != -1)
macroOptions.replaceAll(" interpolate", " interpolation=Bilinear");
else if (macroOptions.indexOf(" interpolation=") == -1)
macroOptions = macroOptions + " interpolation=None";
Macro.setOptions(macroOptions);
}
gd = new GenericDialog("Rotate", IJ.getInstance());
gd.addNumericField("Angle (degrees):", angle, (int) angle == angle ? 1 : 2);
gd.addNumericField("Grid Lines:", gridLines, 0);
gd.addChoice("Interpolation:", methods, methods[interpolationMethod]);
if (bitDepth == 8 || bitDepth == 24)
gd.addCheckbox("Fill with Background Color", fillWithBackground);
if (canEnlarge) gd.addCheckbox("Enlarge Image to Fit Result", enlarge);
else enlarge = false;
gd.addPreviewCheckbox(pfr);
gd.addDialogListener(this);
gd.showDialog();
drawGridLines(0);
if (gd.wasCanceled()) {
return DONE;
}
if (!enlarge) flags |= KEEP_PREVIEW; // standard filter without enlarge
else if (imp.getStackSize() == 1) flags |= NO_CHANGES; // undoable as a "compound filter"
return IJ.setupDialog(imp, flags);
}
/**
* Builds dialog to query users for projection parameters.
*
* @param start starting slice to display
* @param stop last slice
*/
protected GenericDialog buildControlDialog(int start, int stop) {
GenericDialog gd = new GenericDialog("ZProjection", IJ.getInstance());
gd.addNumericField("Start slice:", startSlice, 0 /*digits*/);
gd.addNumericField("Stop slice:", stopSlice, 0 /*digits*/);
gd.addChoice("Projection type", METHODS, METHODS[method]);
if (isHyperstack && imp.getNFrames() > 1 && imp.getNSlices() > 1)
gd.addCheckbox("All time frames", allTimeFrames);
return gd;
}
public ImageCanvas(ImagePlus imp) {
this.imp = imp;
ij = IJ.getInstance();
int width = imp.getWidth();
int height = imp.getHeight();
imageWidth = width;
imageHeight = height;
srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
setDrawingSize(imageWidth, (int) (imageHeight));
magnification = 1.0;
addMouseListener(this);
addMouseMotionListener(this);
addKeyListener(ij); // ImageJ handles keyboard shortcuts
setFocusTraversalKeysEnabled(false);
}
// Miscellaneous Options
void miscOptions() {
String key = IJ.isMacintosh() ? "command" : "control";
GenericDialog gd = new GenericDialog("Miscellaneous Options", IJ.getInstance());
gd.addStringField("Divide by zero value:", "" + FloatBlitter.divideByZeroValue, 10);
gd.addCheckbox("Use pointer cursor", Prefs.usePointerCursor);
gd.addCheckbox("Hide \"Process Stack?\" dialog", IJ.hideProcessStackDialog);
gd.addCheckbox("Require " + key + " key for shortcuts", Prefs.requireControlKey);
gd.addCheckbox("Move isolated plugins to Misc. menu", Prefs.moveToMisc);
if (!IJ.isMacOSX()) gd.addCheckbox("Run single instance listener", Prefs.runSocketListener);
gd.addCheckbox("Enhanced line tool", Prefs.enhancedLineTool);
gd.addCheckbox("Reverse CZT order of \">\" and \"<\"", Prefs.reverseNextPreviousOrder);
gd.addCheckbox("Debug mode", IJ.debugMode);
gd.addHelp(IJ.URL + "/docs/menus/edit.html#misc");
gd.showDialog();
if (gd.wasCanceled()) return;
String divValue = gd.getNextString();
if (divValue.equalsIgnoreCase("infinity") || divValue.equalsIgnoreCase("infinite"))
FloatBlitter.divideByZeroValue = Float.POSITIVE_INFINITY;
else if (divValue.equalsIgnoreCase("NaN")) FloatBlitter.divideByZeroValue = Float.NaN;
else if (divValue.equalsIgnoreCase("max")) FloatBlitter.divideByZeroValue = Float.MAX_VALUE;
else {
Float f;
try {
f = new Float(divValue);
} catch (NumberFormatException e) {
f = null;
}
if (f != null) FloatBlitter.divideByZeroValue = f.floatValue();
}
IJ.register(FloatBlitter.class);
Prefs.usePointerCursor = gd.getNextBoolean();
IJ.hideProcessStackDialog = gd.getNextBoolean();
Prefs.requireControlKey = gd.getNextBoolean();
Prefs.moveToMisc = gd.getNextBoolean();
if (!IJ.isMacOSX()) Prefs.runSocketListener = gd.getNextBoolean();
Prefs.enhancedLineTool = gd.getNextBoolean();
Prefs.reverseNextPreviousOrder = gd.getNextBoolean();
IJ.setDebugMode(gd.getNextBoolean());
}
/** Restores original disk or network version of image. */
public void revertToSaved(ImagePlus imp) {
Image img;
ProgressBar pb = IJ.getInstance().getProgressBar();
ImageProcessor ip;
String path = fi.directory + fi.fileName;
if (fi.fileFormat == fi.GIF_OR_JPG) {
// restore gif or jpg
img = Toolkit.getDefaultToolkit().createImage(path);
imp.setImage(img);
if (imp.getType() == ImagePlus.COLOR_RGB) Opener.convertGrayJpegTo8Bits(imp);
return;
}
if (fi.fileFormat == fi.DICOM) {
// restore DICOM
ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.DICOM", path);
if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
return;
}
if (fi.fileFormat == fi.BMP) {
// restore BMP
ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.BMP_Reader", path);
if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
return;
}
if (fi.fileFormat == fi.PGM) {
// restore PGM
ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.PGM_Reader", path);
if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
return;
}
if (fi.fileFormat == fi.ZIP_ARCHIVE) {
// restore ".zip" file
ImagePlus imp2 = (new Opener()).openZip(path);
if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
return;
}
// restore PNG or another image opened using ImageIO
if (fi.fileFormat == fi.IMAGEIO) {
ImagePlus imp2 = (new Opener()).openUsingImageIO(path);
if (imp2 != null) imp.setProcessor(null, imp2.getProcessor());
return;
}
if (fi.nImages > 1) return;
ColorModel cm;
if (fi.url == null || fi.url.equals("")) IJ.showStatus("Loading: " + path);
else IJ.showStatus("Loading: " + fi.url + fi.fileName);
Object pixels = readPixels(fi);
if (pixels == null) return;
cm = createColorModel(fi);
switch (fi.fileType) {
case FileInfo.GRAY8:
case FileInfo.COLOR8:
case FileInfo.BITMAP:
ip = new ByteProcessor(width, height, (byte[]) pixels, cm);
imp.setProcessor(null, ip);
break;
case FileInfo.GRAY16_SIGNED:
case FileInfo.GRAY16_UNSIGNED:
case FileInfo.GRAY12_UNSIGNED:
ip = new ShortProcessor(width, height, (short[]) pixels, cm);
imp.setProcessor(null, ip);
break;
case FileInfo.GRAY32_INT:
case FileInfo.GRAY32_FLOAT:
ip = new FloatProcessor(width, height, (float[]) pixels, cm);
imp.setProcessor(null, ip);
break;
case FileInfo.RGB:
case FileInfo.BGR:
case FileInfo.ARGB:
case FileInfo.ABGR:
case FileInfo.RGB_PLANAR:
img =
Toolkit.getDefaultToolkit()
.createImage(new MemoryImageSource(width, height, (int[]) pixels, 0, width));
imp.setImage(img);
break;
}
}
public void run(String arg) {
GenericDialog gd = new GenericDialog("Options");
double sfreq = 20000.0;
gd.addNumericField("Sampling Frequency?", sfreq, 1, 10, null);
String[] psfchoice = {"3D Gaussian", "Gaus-Lorentz^2", "2D Gaussian"};
gd.addChoice("PSF Type?", psfchoice, psfchoice[0]);
String[] filetypechoice = {
"Confocor 3 raw", "Short binary trajectory", "PlotWindow trajectory", "Ascii Text File"
};
gd.addChoice("File Type?", filetypechoice, filetypechoice[0]);
boolean ch2green = true;
gd.addCheckbox("Ch2 is green?", ch2green);
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
sfreq = gd.getNextNumber();
int psfflag = gd.getNextChoiceIndex();
int fileflag = gd.getNextChoiceIndex();
ch2green = gd.getNextBoolean();
int nfiles = 0;
Object[] histograms = null;
int xmax = 0;
int ymax = 0;
String[] names = null;
if (fileflag < 2) {
jdataio ioclass = new jdataio();
File[] filearray = ioclass.openfiles(OpenDialog.getDefaultDirectory(), IJ.getInstance());
if (filearray.length == 0) {
return;
}
String dir = filearray[0].getAbsolutePath();
int sepindex = dir.lastIndexOf(File.separator);
String newdir = dir.substring(0, sepindex + 1);
OpenDialog.setDefaultDirectory(newdir);
nfiles = filearray.length / 2;
if (nfiles > 25) {
nfiles = 25;
}
histograms = new Object[nfiles];
names = organize_c3_files(filearray);
for (int i = 0; i < nfiles; i++) {
try {
int length1 = (int) (((double) filearray[2 * i].length() - 128.0) / 4.0);
int length2 = (int) (((double) filearray[2 * i + 1].length() - 128.0) / 4.0);
int length3 = (int) (((double) filearray[2 * i].length()) / 2.0);
int length4 = (int) (((double) filearray[2 * i + 1].length()) / 2.0);
InputStream instream = new BufferedInputStream(new FileInputStream(filearray[2 * i]));
InputStream instream2 =
new BufferedInputStream(new FileInputStream(filearray[2 * i + 1]));
if (fileflag == 0) {
int[] pmdata = new int[length1];
int[] pmdata2 = new int[length2];
if (!ioclass.skipstreambytes(instream, 128)) {
showioerror();
instream.close();
return;
}
if (!ioclass.skipstreambytes(instream2, 128)) {
showioerror();
instream2.close();
return;
}
if (!ioclass.readintelintfile(instream, length1, pmdata)) {
showioerror();
instream.close();
return;
}
if (!ioclass.readintelintfile(instream2, length2, pmdata2)) {
showioerror();
instream2.close();
return;
}
if (ch2green) {
histograms[i] = (new pmodeconvert()).pm2pch(pmdata2, pmdata, sfreq, 20000000);
} else {
histograms[i] = (new pmodeconvert()).pm2pch(pmdata, pmdata2, sfreq, 20000000);
}
} else {
float[] tmdata = new float[length3];
float[] tmdata2 = new float[length4];
if (!ioclass.readintelshortfile(instream, length3, tmdata)) {
showioerror();
instream.close();
return;
}
if (!ioclass.readintelshortfile(instream2, length4, tmdata2)) {
showioerror();
instream2.close();
return;
}
if (ch2green) {
histograms[i] = (new pmodeconvert()).create_2Dhistogram(tmdata2, tmdata);
} else {
histograms[i] = (new pmodeconvert()).create_2Dhistogram(tmdata, tmdata2);
}
}
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
instream.close();
instream2.close();
} catch (IOException e) {
showioerror();
return;
}
}
} else {
if (fileflag == 2) {
ImageWindow iw = WindowManager.getCurrentWindow();
float[][] trajectories = (float[][]) jutils.runPW4VoidMethod(iw, "getYValues");
float[][] tempxvals = (float[][]) jutils.runPW4VoidMethod(iw, "getXValues");
sfreq = 1.0 / ((double) tempxvals[0][1]);
nfiles = trajectories.length / 2;
if (nfiles > 25) {
nfiles = 25;
}
names = new String[nfiles + 1];
names[nfiles] = "avg";
histograms = new Object[nfiles];
for (int i = 0; i < nfiles; i++) {
names[i] = "trajectory " + (i + 1);
if (ch2green) {
histograms[i] =
(new pmodeconvert())
.create_2Dhistogram(trajectories[2 * i + 1], trajectories[2 * i]);
} else {
histograms[i] =
(new pmodeconvert())
.create_2Dhistogram(trajectories[2 * i], trajectories[2 * i + 1]);
}
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
}
} else {
// here we read tab delimited lines from files
jdataio ioclass = new jdataio();
File[] filearray = ioclass.openfiles(OpenDialog.getDefaultDirectory(), IJ.getInstance());
if (filearray.length == 0) {
return;
}
String dir = filearray[0].getAbsolutePath();
int sepindex = dir.lastIndexOf(File.separator);
String newdir = dir.substring(0, sepindex + 1);
OpenDialog.setDefaultDirectory(newdir);
nfiles = filearray.length;
if (nfiles > 25) {
nfiles = 25;
}
histograms = new Object[nfiles];
names = new String[nfiles + 1];
names[nfiles] = "avg";
for (int i = 0; i < nfiles; i++) {
try {
names[i] = filearray[i].getName();
BufferedReader d = new BufferedReader(new FileReader(filearray[i]));
String[] lines = new String[256];
int counter = 0;
do {
lines[counter] = d.readLine();
counter++;
} while ((lines[counter - 1] != null && lines[counter - 1] != "") && counter < 256);
int numcolumns = 0;
for (int j = 0; j < counter - 1; j++) {
int temp = getncolumns(lines[j]);
if (temp > numcolumns) {
numcolumns = temp;
}
}
float[][] temphist2 = null;
if (ch2green) {
temphist2 = new float[numcolumns][counter - 1];
} else {
temphist2 = new float[counter - 1][numcolumns];
}
for (int k = 0; k < counter - 1; k++) {
float[] temp = tab_delim2float(lines[k]);
for (int j = 0; j < numcolumns; j++) {
if (ch2green) {
temphist2[j][k] = temp[j];
} else {
temphist2[k][j] = temp[j];
}
}
}
histograms[i] = temphist2;
d.close();
} catch (IOException e) {
showioerror();
return;
}
}
for (int i = 0; i < nfiles; i++) {
if (((float[][]) histograms[i]).length > xmax) {
xmax = ((float[][]) histograms[i]).length;
}
if (((float[][]) histograms[i])[0].length > ymax) {
ymax = ((float[][]) histograms[i])[0].length;
}
}
}
}
// note that here x is green and y is red
float[][][] pch = new float[nfiles][xmax][ymax];
for (int i = 0; i < nfiles; i++) {
for (int j = 0; j < ((float[][]) histograms[i]).length; j++) {
for (int k = 0; k < ((float[][]) histograms[i])[j].length; k++) {
pch[i][j][k] = ((float[][]) histograms[i])[j][k];
}
}
}
final PCH2DFitWindow cw = new PCH2DFitWindow();
cw.init(names, pch, psfflag);
final Frame f = new Frame("PCH 2D Analysis");
f.setLocation(10, 10);
f.addWindowListener(
new WindowAdapter() {
public void windowClosing(WindowEvent e) {
f.dispose();
}
});
f.add(cw);
f.pack();
f.setResizable(false);
Insets ins = f.getInsets();
cw.totalSize.height = PCH2DFitWindow.H + ins.bottom + ins.top + 65;
cw.totalSize.width = PCH2DFitWindow.WR + ins.left + ins.right;
f.setSize(cw.totalSize);
f.setVisible(true);
cw.requestFocus();
}
public static void main(String args[]) {
if (System.getProperty("java.version").substring(0, 3).compareTo("1.5") < 0) {
javax.swing.JOptionPane.showMessageDialog(
null, "ImageJ " + VERSION + " requires Java 1.5 or later.");
System.exit(0);
}
boolean noGUI = false;
int mode = STANDALONE;
arguments = args;
// System.setProperty("file.encoding", "UTF-8");
int nArgs = args != null ? args.length : 0;
boolean commandLine = false;
for (int i = 0; i < nArgs; i++) {
String arg = args[i];
if (arg == null) continue;
if (args[i].startsWith("-")) {
if (args[i].startsWith("-batch")) noGUI = true;
else if (args[i].startsWith("-debug")) IJ.setDebugMode(true);
else if (args[i].startsWith("-ijpath") && i + 1 < nArgs) {
if (IJ.debugMode) IJ.log("-ijpath: " + args[i + 1]);
Prefs.setHomeDir(args[i + 1]);
commandLine = true;
args[i + 1] = null;
} else if (args[i].startsWith("-port")) {
int delta = (int) Tools.parseDouble(args[i].substring(5, args[i].length()), 0.0);
commandLine = true;
if (delta == 0) mode = EMBEDDED;
else if (delta > 0 && DEFAULT_PORT + delta < 65536) port = DEFAULT_PORT + delta;
}
}
}
// If existing ImageJ instance, pass arguments to it and quit.
boolean passArgs = mode == STANDALONE && !noGUI;
if (IJ.isMacOSX() && !commandLine) passArgs = false;
if (passArgs && isRunning(args)) return;
ImageJ ij = IJ.getInstance();
if (!noGUI && (ij == null || (ij != null && !ij.isShowing()))) {
ij = new ImageJ(null, mode);
ij.exitWhenQuitting = true;
}
int macros = 0;
for (int i = 0; i < nArgs; i++) {
String arg = args[i];
if (arg == null) continue;
if (arg.startsWith("-")) {
if ((arg.startsWith("-macro") || arg.startsWith("-batch")) && i + 1 < nArgs) {
String arg2 = i + 2 < nArgs ? args[i + 2] : null;
Prefs.commandLineMacro = true;
if (noGUI && args[i + 1].endsWith(".js")) Interpreter.batchMode = true;
IJ.runMacroFile(args[i + 1], arg2);
break;
} else if (arg.startsWith("-eval") && i + 1 < nArgs) {
String rtn = IJ.runMacro(args[i + 1]);
if (rtn != null) System.out.print(rtn);
args[i + 1] = null;
} else if (arg.startsWith("-run") && i + 1 < nArgs) {
IJ.run(args[i + 1]);
args[i + 1] = null;
}
} else if (macros == 0 && (arg.endsWith(".ijm") || arg.endsWith(".txt"))) {
IJ.runMacroFile(arg);
macros++;
} else if (arg.length() > 0 && arg.indexOf("ij.ImageJ") == -1) {
File file = new File(arg);
IJ.open(file.getAbsolutePath());
}
}
if (IJ.debugMode && IJ.getInstance() == null) new JavaProperties().run("");
if (noGUI) System.exit(0);
}
/**
* Performs particle analysis on the specified ImagePlus and ImageProcessor. Returns false if
* there is an error.
*/
public boolean analyze(ImagePlus imp, ImageProcessor ip) {
if (this.imp == null) this.imp = imp;
showResults = (options & SHOW_RESULTS) != 0;
excludeEdgeParticles = (options & EXCLUDE_EDGE_PARTICLES) != 0;
resetCounter = (options & CLEAR_WORKSHEET) != 0;
showProgress = (options & SHOW_PROGRESS) != 0;
floodFill = (options & INCLUDE_HOLES) == 0;
recordStarts = (options & RECORD_STARTS) != 0;
addToManager = (options & ADD_TO_MANAGER) != 0;
displaySummary = (options & DISPLAY_SUMMARY) != 0;
inSituShow = (options & IN_SITU_SHOW) != 0;
outputImage = null;
ip.snapshot();
ip.setProgressBar(null);
if (Analyzer.isRedirectImage()) {
redirectImp = Analyzer.getRedirectImage(imp);
if (redirectImp == null) return false;
int depth = redirectImp.getStackSize();
if (depth > 1 && depth == imp.getStackSize()) {
ImageStack redirectStack = redirectImp.getStack();
redirectIP = redirectStack.getProcessor(imp.getCurrentSlice());
} else redirectIP = redirectImp.getProcessor();
} else if (imp.getType() == ImagePlus.COLOR_RGB) {
ImagePlus original = (ImagePlus) imp.getProperty("OriginalImage");
if (original != null
&& original.getWidth() == imp.getWidth()
&& original.getHeight() == imp.getHeight()) {
redirectImp = original;
redirectIP = original.getProcessor();
}
}
if (!setThresholdLevels(imp, ip)) return false;
width = ip.getWidth();
height = ip.getHeight();
if (!(showChoice == NOTHING || showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS)) {
blackBackground = Prefs.blackBackground && inSituShow;
if (slice == 1) outlines = new ImageStack(width, height);
if (showChoice == ROI_MASKS) drawIP = new ShortProcessor(width, height);
else drawIP = new ByteProcessor(width, height);
drawIP.setLineWidth(lineWidth);
if (showChoice == ROI_MASKS) {
} // Place holder for now...
else if (showChoice == MASKS && !blackBackground) drawIP.invertLut();
else if (showChoice == OUTLINES) {
if (!inSituShow) {
if (customLut == null) makeCustomLut();
drawIP.setColorModel(customLut);
}
drawIP.setFont(new Font("SansSerif", Font.PLAIN, fontSize));
if (fontSize > 12 && inSituShow) drawIP.setAntialiasedText(true);
}
outlines.addSlice(null, drawIP);
if (showChoice == ROI_MASKS || blackBackground) {
drawIP.setColor(Color.black);
drawIP.fill();
drawIP.setColor(Color.white);
} else {
drawIP.setColor(Color.white);
drawIP.fill();
drawIP.setColor(Color.black);
}
}
calibration = redirectImp != null ? redirectImp.getCalibration() : imp.getCalibration();
if (rt == null) {
rt = Analyzer.getResultsTable();
analyzer = new Analyzer(imp);
} else analyzer = new Analyzer(imp, measurements, rt);
if (resetCounter && slice == 1) {
if (!Analyzer.resetCounter()) return false;
}
beginningCount = Analyzer.getCounter();
byte[] pixels = null;
if (ip instanceof ByteProcessor) pixels = (byte[]) ip.getPixels();
if (r == null) {
r = ip.getRoi();
mask = ip.getMask();
if (displaySummary) {
if (mask != null) totalArea = ImageStatistics.getStatistics(ip, AREA, calibration).area;
else totalArea = r.width * calibration.pixelWidth * r.height * calibration.pixelHeight;
}
}
minX = r.x;
maxX = r.x + r.width;
minY = r.y;
maxY = r.y + r.height;
if (r.width < width || r.height < height || mask != null) {
if (!eraseOutsideRoi(ip, r, mask)) return false;
}
int offset;
double value;
int inc = Math.max(r.height / 25, 1);
int mi = 0;
ImageWindow win = imp.getWindow();
if (win != null) win.running = true;
if (measurements == 0) measurements = Analyzer.getMeasurements();
if (showChoice == ELLIPSES) measurements |= ELLIPSE;
measurements &= ~LIMIT; // ignore "Limit to Threshold"
roiNeedsImage =
(measurements & PERIMETER) != 0
|| (measurements & SHAPE_DESCRIPTORS) != 0
|| (measurements & FERET) != 0;
particleCount = 0;
wand = new Wand(ip);
pf = new PolygonFiller();
if (floodFill) {
ImageProcessor ipf = ip.duplicate();
ipf.setValue(fillColor);
ff = new FloodFiller(ipf);
}
roiType = Wand.allPoints() ? Roi.FREEROI : Roi.TRACED_ROI;
for (int y = r.y; y < (r.y + r.height); y++) {
offset = y * width;
for (int x = r.x; x < (r.x + r.width); x++) {
if (pixels != null) value = pixels[offset + x] & 255;
else if (imageType == SHORT) value = ip.getPixel(x, y);
else value = ip.getPixelValue(x, y);
if (value >= level1 && value <= level2) analyzeParticle(x, y, imp, ip);
}
if (showProgress && ((y % inc) == 0)) IJ.showProgress((double) (y - r.y) / r.height);
if (win != null) canceled = !win.running;
if (canceled) {
Macro.abort();
break;
}
}
if (showProgress) IJ.showProgress(1.0);
if (showResults) rt.updateResults();
imp.killRoi();
ip.resetRoi();
ip.reset();
if (displaySummary && IJ.getInstance() != null) updateSliceSummary();
if (addToManager && roiManager != null) roiManager.setEditMode(imp, true);
maxParticleCount = (particleCount > maxParticleCount) ? particleCount : maxParticleCount;
totalCount += particleCount;
if (!canceled) showResults();
return true;
}
String getInfo(ImagePlus imp, ImageProcessor ip) {
String s = new String("");
if (IJ.getInstance() != null) s += IJ.getInstance().getInfo() + "\n \n";
s += "Title: " + imp.getTitle() + "\n";
Calibration cal = imp.getCalibration();
int stackSize = imp.getStackSize();
int channels = imp.getNChannels();
int slices = imp.getNSlices();
int frames = imp.getNFrames();
int digits = imp.getBitDepth() == 32 ? 4 : 0;
int dp, dp2;
boolean nonUniformUnits = !cal.getXUnit().equals(cal.getYUnit());
String xunit = cal.getXUnit();
String yunit = cal.getYUnit();
String zunit = cal.getZUnit();
if (cal.scaled()) {
String xunits = cal.getUnits();
String yunits = xunits;
String zunits = xunits;
if (nonUniformUnits) {
xunits = xunit;
yunits = yunit;
zunits = zunit;
}
double pw = imp.getWidth() * cal.pixelWidth;
double ph = imp.getHeight() * cal.pixelHeight;
s += "Width: " + d2s(pw) + " " + xunits + " (" + imp.getWidth() + ")\n";
s += "Height: " + d2s(ph) + " " + yunits + " (" + imp.getHeight() + ")\n";
if (slices > 1) {
double pd = slices * cal.pixelDepth;
s += "Depth: " + d2s(pd) + " " + zunits + " (" + slices + ")\n";
}
s += "Size: " + ImageWindow.getImageSize(imp) + "\n";
double xResolution = 1.0 / cal.pixelWidth;
double yResolution = 1.0 / cal.pixelHeight;
if (xResolution == yResolution)
s += "Resolution: " + d2s(xResolution) + " pixels per " + xunit + "\n";
else {
s += "X Resolution: " + d2s(xResolution) + " pixels per " + xunit + "\n";
s += "Y Resolution: " + d2s(yResolution) + " pixels per " + yunit + "\n";
}
} else {
s += "Width: " + imp.getWidth() + " pixels\n";
s += "Height: " + imp.getHeight() + " pixels\n";
if (stackSize > 1) s += "Depth: " + slices + " pixels\n";
s += "Size: " + ImageWindow.getImageSize(imp) + "\n";
}
if (stackSize > 1) {
String vunit = cal.getUnit() + "^3";
if (nonUniformUnits) vunit = "(" + xunit + " x " + yunit + " x " + zunit + ")";
s +=
"Voxel size: "
+ d2s(cal.pixelWidth)
+ "x"
+ d2s(cal.pixelHeight)
+ "x"
+ d2s(cal.pixelDepth)
+ " "
+ vunit
+ "\n";
} else {
String punit = cal.getUnit() + "^2";
if (nonUniformUnits) punit = "(" + xunit + " x " + yunit + ")";
dp = Tools.getDecimalPlaces(cal.pixelWidth, cal.pixelHeight);
s += "Pixel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + " " + punit + "\n";
}
s += "ID: " + imp.getID() + "\n";
int type = imp.getType();
switch (type) {
case ImagePlus.GRAY8:
s += "Bits per pixel: 8 ";
String lut = "LUT";
if (imp.getProcessor().isColorLut()) lut = "color " + lut;
else lut = "grayscale " + lut;
if (imp.isInvertedLut()) lut = "inverting " + lut;
s += "(" + lut + ")\n";
if (imp.getNChannels() > 1) s += displayRanges(imp);
else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n";
break;
case ImagePlus.GRAY16:
case ImagePlus.GRAY32:
if (type == ImagePlus.GRAY16) {
String sign = cal.isSigned16Bit() ? "signed" : "unsigned";
s += "Bits per pixel: 16 (" + sign + ")\n";
} else s += "Bits per pixel: 32 (float)\n";
if (imp.getNChannels() > 1) s += displayRanges(imp);
else {
s += "Display range: ";
double min = ip.getMin();
double max = ip.getMax();
if (cal.calibrated()) {
min = cal.getCValue((int) min);
max = cal.getCValue((int) max);
}
s += d2s(min) + " - " + d2s(max) + "\n";
}
break;
case ImagePlus.COLOR_256:
s += "Bits per pixel: 8 (color LUT)\n";
break;
case ImagePlus.COLOR_RGB:
s += "Bits per pixel: 32 (RGB)\n";
break;
}
double interval = cal.frameInterval;
double fps = cal.fps;
if (stackSize > 1) {
ImageStack stack = imp.getStack();
int slice = imp.getCurrentSlice();
String number = slice + "/" + stackSize;
String label = stack.getShortSliceLabel(slice);
if (label != null && label.length() > 0) label = " (" + label + ")";
else label = "";
if (interval > 0.0 || fps != 0.0) {
s += "Frame: " + number + label + "\n";
if (fps != 0.0) {
String sRate =
Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5);
s += "Frame rate: " + sRate + " fps\n";
}
if (interval != 0.0)
s +=
"Frame interval: "
+ ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5))
+ " "
+ cal.getTimeUnit()
+ "\n";
} else s += "Image: " + number + label + "\n";
if (imp.isHyperStack()) {
if (channels > 1) s += " Channel: " + imp.getChannel() + "/" + channels + "\n";
if (slices > 1) s += " Slice: " + imp.getSlice() + "/" + slices + "\n";
if (frames > 1) s += " Frame: " + imp.getFrame() + "/" + frames + "\n";
}
if (imp.isComposite()) {
if (!imp.isHyperStack() && channels > 1) s += " Channels: " + channels + "\n";
String mode = ((CompositeImage) imp).getModeAsString();
s += " Composite mode: \"" + mode + "\"\n";
}
}
if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No threshold\n";
else {
double lower = ip.getMinThreshold();
double upper = ip.getMaxThreshold();
if (cal.calibrated()) {
lower = cal.getCValue((int) lower);
upper = cal.getCValue((int) upper);
}
s += "Threshold: " + d2s(lower) + "-" + d2s(upper) + "\n";
}
ImageCanvas ic = imp.getCanvas();
double mag = ic != null ? ic.getMagnification() : 1.0;
if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n";
if (cal.calibrated()) {
s += " \n";
int curveFit = cal.getFunction();
s += "Calibration function: ";
if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n";
else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n";
else s += CurveFitter.fList[curveFit] + "\n";
double[] c = cal.getCoefficients();
if (c != null) {
s += " a: " + IJ.d2s(c[0], 6) + "\n";
s += " b: " + IJ.d2s(c[1], 6) + "\n";
if (c.length >= 3) s += " c: " + IJ.d2s(c[2], 6) + "\n";
if (c.length >= 4) s += " c: " + IJ.d2s(c[3], 6) + "\n";
if (c.length >= 5) s += " c: " + IJ.d2s(c[4], 6) + "\n";
}
s += " Unit: \"" + cal.getValueUnit() + "\"\n";
} else s += "Uncalibrated\n";
FileInfo fi = imp.getOriginalFileInfo();
if (fi != null) {
if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n";
else if (fi.directory != null && fi.fileName != null)
s += "Path: " + fi.directory + fi.fileName + "\n";
}
ImageWindow win = imp.getWindow();
if (win != null) {
Point loc = win.getLocation();
Dimension screen = IJ.getScreenSize();
s +=
"Screen location: "
+ loc.x
+ ","
+ loc.y
+ " ("
+ screen.width
+ "x"
+ screen.height
+ ")\n";
}
String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : "";
String origin = d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin;
if (!origin.equals("0,0") || cal.getInvertY()) s += "Coordinate origin: " + origin + "\n";
if (cal.getInvertY()) s += "Inverted y coordinates\n";
Overlay overlay = imp.getOverlay();
if (overlay != null) {
String hidden = imp.getHideOverlay() ? " (hidden)" : " ";
int n = overlay.size();
String elements = n == 1 ? " element" : " elements";
s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n";
} else s += "No overlay\n";
Roi roi = imp.getRoi();
if (roi == null) {
if (cal.calibrated()) s += " \n";
s += "No selection\n";
} else if (roi instanceof EllipseRoi) {
s += "\nElliptical selection\n";
double[] p = ((EllipseRoi) roi).getParams();
double dx = p[2] - p[0];
double dy = p[3] - p[1];
double major = Math.sqrt(dx * dx + dy * dy);
s += " Major: " + IJ.d2s(major, 2) + "\n";
s += " Minor: " + IJ.d2s(major * p[4], 2) + "\n";
s += " X1: " + IJ.d2s(p[0], 2) + "\n";
s += " Y1: " + IJ.d2s(p[1], 2) + "\n";
s += " X2: " + IJ.d2s(p[2], 2) + "\n";
s += " Y2: " + IJ.d2s(p[3], 2) + "\n";
s += " Aspect ratio: " + IJ.d2s(p[4], 2) + "\n";
} else {
s += " \n";
s += roi.getTypeAsString() + " Selection";
String points = null;
if (roi instanceof PointRoi) {
int npoints = ((PolygonRoi) roi).getNCoordinates();
String suffix = npoints > 1 ? "s)" : ")";
points = " (" + npoints + " point" + suffix;
}
String name = roi.getName();
if (name != null) {
s += " (\"" + name + "\")";
if (points != null) s += "\n " + points;
} else if (points != null) s += points;
s += "\n";
Rectangle r = roi.getBounds();
if (roi instanceof Line) {
Line line = (Line) roi;
s += " X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n";
s += " Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n";
s += " X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n";
s += " Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n";
} else if (cal.scaled()) {
s += " X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n";
s += " Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n";
s += " Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n";
s += " Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n";
} else {
s += " X: " + r.x + "\n";
s += " Y: " + yy(r.y, imp) + "\n";
s += " Width: " + r.width + "\n";
s += " Height: " + r.height + "\n";
}
}
return s;
}
/** Ask for parameters and then execute. */
public void run(String arg) {
// 1 - Obtain the currently active image:
ImagePlus imp = IJ.getImage();
if (null == imp) {
IJ.showMessage("There must be at least one image open");
return;
}
if (imp.getBitDepth() != 8) {
IJ.showMessage("Error", "Only 8-bit images are supported");
return;
}
// 2 - Ask for parameters:
GenericDialog gd = new GenericDialog("Auto Local Threshold");
String[] methods = {
"Try all",
"Bernsen",
"Contrast",
"Mean",
"Median",
"MidGrey",
"Niblack",
"Otsu",
"Phansalkar",
"Sauvola"
};
gd.addMessage("Auto Local Threshold v1.5");
gd.addChoice("Method", methods, methods[0]);
gd.addNumericField("Radius", 15, 0);
gd.addMessage("Special paramters (if different from default)");
gd.addNumericField("Parameter_1", 0, 0);
gd.addNumericField("Parameter_2", 0, 0);
gd.addCheckbox("White objects on black background", true);
if (imp.getStackSize() > 1) {
gd.addCheckbox("Stack", false);
}
gd.addMessage("Thresholded result is always shown in white [255].");
gd.showDialog();
if (gd.wasCanceled()) return;
// 3 - Retrieve parameters from the dialog
String myMethod = gd.getNextChoice();
int radius = (int) gd.getNextNumber();
double par1 = (double) gd.getNextNumber();
double par2 = (double) gd.getNextNumber();
boolean doIwhite = gd.getNextBoolean();
boolean doIstack = false;
int stackSize = imp.getStackSize();
if (stackSize > 1) doIstack = gd.getNextBoolean();
// 4 - Execute!
// long start = System.currentTimeMillis();
if (myMethod.equals("Try all")) {
ImageProcessor ip = imp.getProcessor();
int xe = ip.getWidth();
int ye = ip.getHeight();
int ml = methods.length;
ImagePlus imp2, imp3;
ImageStack tstack = null, stackNew;
if (stackSize > 1 && doIstack) {
boolean doItAnyway = true;
if (stackSize > 25) {
YesNoCancelDialog d =
new YesNoCancelDialog(
IJ.getInstance(),
"Auto Local Threshold",
"You might run out of memory.\n \nDisplay "
+ stackSize
+ " slices?\n \n \'No\' will process without display and\noutput results to the log window.");
if (!d.yesPressed()) {
// doIlog=true; //will show in the log window
doItAnyway = false;
}
if (d.cancelPressed()) return;
}
for (int j = 1; j <= stackSize; j++) {
imp.setSlice(j);
ip = imp.getProcessor();
tstack = new ImageStack(xe, ye);
for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
imp2 = new ImagePlus("Auto Threshold", tstack);
imp2.updateAndDraw();
for (int k = 1; k < ml; k++) {
imp2.setSlice(k);
Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
}
// if (doItAnyway){
CanvasResizer cr = new CanvasResizer();
stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
imp3 = new ImagePlus("Auto Threshold", stackNew);
imp3.updateAndDraw();
MontageMaker mm = new MontageMaker();
mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); // 3 columns and 3 rows
}
imp.setSlice(1);
// if (doItAnyway)
IJ.run("Images to Stack", "method=[Copy (center)] title=Montage");
return;
} else { // single image try all
tstack = new ImageStack(xe, ye);
for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
imp2 = new ImagePlus("Auto Threshold", tstack);
imp2.updateAndDraw();
for (int k = 1; k < ml; k++) {
imp2.setSlice(k);
// IJ.log("analyzing slice with "+methods[k]);
Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
}
// imp2.setSlice(1);
CanvasResizer cr = new CanvasResizer();
stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
imp3 = new ImagePlus("Auto Threshold", stackNew);
imp3.updateAndDraw();
MontageMaker mm = new MontageMaker();
mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true);
return;
}
} else { // selected a method
if (stackSize > 1 && doIstack) { // whole stack
// if (doIstackHistogram) {// one global histogram
// Object[] result = exec(imp, myMethod, noWhite, noBlack, doIwhite, doIset, doIlog,
// doIstackHistogram );
// }
// else{ // slice by slice
for (int k = 1; k <= stackSize; k++) {
imp.setSlice(k);
Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
}
// }
imp.setSlice(1);
} else { // just one slice
Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
}
// 5 - If all went well, show the image:
// not needed here as the source image is binarised
}
}
public BandAdjuster() {
super("Threshold Colour");
if (instance != null) {
instance.toFront();
return;
}
imp = WindowManager.getCurrentImage();
if (imp == null) {
IJ.beep();
IJ.showStatus("No image");
return;
}
IJ.run("Select None");
thread = new Thread(this, "BandAdjuster");
WindowManager.addWindow(this);
instance = this;
IJ.register(PasteController.class);
ij = IJ.getInstance();
Font font = new Font("SansSerif", Font.PLAIN, 10);
GridBagLayout gridbag = new GridBagLayout();
GridBagConstraints c = new GridBagConstraints();
setLayout(gridbag);
int y = 0;
c.gridx = 0;
c.gridy = y;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(5, 0, 0, 0);
labelh = new Label("Hue", Label.CENTER);
add(labelh, c);
c.gridx = 1;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(7, 0, 0, 0);
labelf = new Label("Filter type", Label.RIGHT);
add(labelf, c);
// plot
c.gridx = 0;
c.gridy = y;
c.gridwidth = 1;
c.fill = c.BOTH;
c.anchor = c.CENTER;
c.insets = new Insets(0, 5, 0, 0);
add(plot, c);
// checkboxes
panelh = new Panel();
filterTypeH = new CheckboxGroup();
bandPassH = new Checkbox("Pass");
bandPassH.setCheckboxGroup(filterTypeH);
bandPassH.addItemListener(this);
panelh.add(bandPassH);
bandStopH = new Checkbox("Stop");
bandStopH.setCheckboxGroup(filterTypeH);
bandStopH.addItemListener(this);
panelh.add(bandStopH);
bandPassH.setState(true);
c.gridx = 1;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(5, 0, 0, 0);
add(panelh, c);
// minHue slider
minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 90 : 100;
c.fill = c.HORIZONTAL;
c.insets = new Insets(5, 5, 0, 0);
add(minSlider, c);
minSlider.addAdjustmentListener(this);
minSlider.setUnitIncrement(1);
// minHue slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 10 : 0;
c.insets = new Insets(5, 0, 0, 0);
label1 = new Label(" ", Label.LEFT);
label1.setFont(font);
add(label1, c);
// maxHue sliderHue
maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y;
c.gridwidth = 1;
c.weightx = 100;
c.insets = new Insets(5, 5, 0, 0);
add(maxSlider, c);
maxSlider.addAdjustmentListener(this);
maxSlider.setUnitIncrement(1);
// maxHue slider label
c.gridx = 1;
c.gridwidth = 1;
c.gridy = y++;
c.weightx = 0;
c.insets = new Insets(5, 0, 0, 0);
label2 = new Label(" ", Label.LEFT);
label2.setFont(font);
add(label2, c);
// =====
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(10, 0, 0, 0);
labels = new Label("Saturation", Label.CENTER);
add(labels, c);
// plot
c.gridx = 0;
c.gridy = y;
c.gridwidth = 1;
c.fill = c.BOTH;
c.anchor = c.CENTER;
c.insets = new Insets(0, 5, 0, 0);
add(splot, c);
// checkboxes
panels = new Panel();
filterTypeS = new CheckboxGroup();
bandPassS = new Checkbox("Pass");
bandPassS.setCheckboxGroup(filterTypeS);
bandPassS.addItemListener(this);
panels.add(bandPassS);
bandStopS = new Checkbox("Stop");
bandStopS.setCheckboxGroup(filterTypeS);
bandStopS.addItemListener(this);
panels.add(bandStopS);
bandPassS.setState(true);
c.gridx = 1;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(5, 0, 0, 0);
add(panels, c);
// minSat slider
minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 90 : 100;
c.fill = c.HORIZONTAL;
c.insets = new Insets(5, 5, 0, 0);
add(minSlider2, c);
minSlider2.addAdjustmentListener(this);
minSlider2.setUnitIncrement(1);
// minSat slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 10 : 0;
c.insets = new Insets(5, 0, 0, 0);
label3 = new Label(" ", Label.LEFT);
label3.setFont(font);
add(label3, c);
// maxSat slider
maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = 100;
c.insets = new Insets(5, 5, 0, 0);
add(maxSlider2, c);
maxSlider2.addAdjustmentListener(this);
maxSlider2.setUnitIncrement(1);
// maxSat slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(5, 0, 0, 0);
label4 = new Label(" ", Label.LEFT);
label4.setFont(font);
add(label4, c);
// =====
c.gridx = 0;
c.gridwidth = 1;
c.gridy = y++;
c.weightx = 0;
c.insets = new Insets(10, 0, 0, 0);
labelb = new Label("Brightness", Label.CENTER);
add(labelb, c);
c.gridx = 0;
c.gridwidth = 1;
c.gridy = y;
c.fill = c.BOTH;
c.anchor = c.CENTER;
c.insets = new Insets(0, 5, 0, 0);
add(bplot, c);
// checkboxes
panelb = new Panel();
filterTypeB = new CheckboxGroup();
bandPassB = new Checkbox("Pass");
bandPassB.setCheckboxGroup(filterTypeB);
bandPassB.addItemListener(this);
panelb.add(bandPassB);
bandStopB = new Checkbox("Stop");
bandStopB.setCheckboxGroup(filterTypeB);
bandStopB.addItemListener(this);
panelb.add(bandStopB);
bandPassB.setState(true);
c.gridx = 1;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(5, 0, 0, 0);
add(panelb, c);
// minBri slider
minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 90 : 100;
c.fill = c.HORIZONTAL;
c.insets = new Insets(5, 5, 0, 0);
add(minSlider3, c);
minSlider3.addAdjustmentListener(this);
minSlider3.setUnitIncrement(1);
// minBri slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = IJ.isMacintosh() ? 10 : 0;
c.insets = new Insets(5, 0, 0, 0);
label5 = new Label(" ", Label.LEFT);
label5.setFont(font);
add(label5, c);
// maxBri slider
maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 1;
c.weightx = 100;
c.insets = new Insets(5, 5, 0, 0);
add(maxSlider3, c);
maxSlider3.addAdjustmentListener(this);
maxSlider3.setUnitIncrement(1);
// maxBri slider label
c.gridx = 1;
c.gridwidth = 1;
c.weightx = 0;
c.insets = new Insets(5, 0, 0, 0);
label6 = new Label(" ", Label.LEFT);
label6.setFont(font);
add(label6, c);
// =====
panelt = new Panel();
threshold = new Checkbox("Threshold");
threshold.addItemListener(this);
panelt.add(threshold);
invert = new Checkbox("Invert");
invert.addItemListener(this);
panelt.add(invert);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(0, 0, 0, 0);
add(panelt, c);
// buttons
panel = new Panel();
// panel.setLayout(new GridLayout(2, 2, 0, 0));
originalB = new Button("Original");
originalB.setEnabled(false);
originalB.addActionListener(this);
originalB.addKeyListener(ij);
panel.add(originalB);
filteredB = new Button("Filtered");
filteredB.setEnabled(false);
filteredB.addActionListener(this);
filteredB.addKeyListener(ij);
panel.add(filteredB);
stackB = new Button("Stack");
stackB.addActionListener(this);
stackB.addKeyListener(ij);
panel.add(stackB);
helpB = new Button("Help");
helpB.addActionListener(this);
helpB.addKeyListener(ij);
panel.add(helpB);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(0, 0, 0, 0);
add(panel, c);
panelMode = new Panel();
sampleB = new Button("Sample");
sampleB.addActionListener(this);
sampleB.addKeyListener(ij);
panelMode.add(sampleB);
colourMode = new CheckboxGroup();
hsb = new Checkbox("HSB");
hsb.setCheckboxGroup(colourMode);
hsb.addItemListener(this);
panelMode.add(hsb);
hsb.setState(true);
rgb = new Checkbox("RGB");
rgb.setCheckboxGroup(colourMode);
rgb.addItemListener(this);
panelMode.add(rgb);
c.gridx = 0;
c.gridy = y++;
c.gridwidth = 2;
c.insets = new Insets(0, 0, 0, 0);
add(panelMode, c);
addKeyListener(ij); // ImageJ handles keyboard shortcuts
pack();
GUI.center(this);
setVisible(true);
ip = setup(imp);
if (ip == null) {
imp.unlock();
IJ.beep();
IJ.showStatus("RGB image cannot be thresholded");
return;
}
thread.start();
}
/*------------------------------------------------------------------*/
public class Differentials_ implements PlugIn {
/* begin class Differentials_ */
/*..................................................................*/
/* Global-scope variables */
/*..................................................................*/
static final int GRADIENT_MAGNITUDE = 1;
static final int GRADIENT_DIRECTION = 2;
static final int LAPLACIAN = 3;
static final int LARGEST_HESSIAN = 4;
static final int SMALLEST_HESSIAN = 5;
static final int HESSIAN_ORIENTATION = 6;
private final double FLT_EPSILON = (double) Float.intBitsToFloat((int) 0x33FFFFFF);
private ImagePlus imp = null;
private ProgressBar progressBar = IJ.getInstance().getProgressBar();
private long lastTime = System.currentTimeMillis();
private int completed = 1;
private int processDuration = 1;
private static int operation = LAPLACIAN;
private int stackSize = 1;
private boolean cancel = false;
/*..................................................................*/
/* Public methods */
/*..................................................................*/
/*------------------------------------------------------------------*/
public void getCrossHessian(ImageProcessor ip, double tolerance) {
getHorizontalGradient(ip, tolerance);
getVerticalGradient(ip, tolerance);
} /* end getCrossHessian */
/*------------------------------------------------------------------*/
public void getHorizontalGradient(ImageProcessor ip, double tolerance) {
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
float[] floatPixels = (float[]) ip.getPixels();
int width = ip.getWidth();
int height = ip.getHeight();
double line[] = new double[width];
for (int y = 0; (y < height); y++) {
getRow(ip, y, line);
getSplineInterpolationCoefficients(line, tolerance);
getGradient(line);
putRow(ip, y, line);
stepProgressBar();
}
} /* end getHorizontalGradient */
/*------------------------------------------------------------------*/
public void getHorizontalHessian(ImageProcessor ip, double tolerance) {
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
float[] floatPixels = (float[]) ip.getPixels();
int width = ip.getWidth();
int height = ip.getHeight();
double line[] = new double[width];
for (int y = 0; (y < height); y++) {
getRow(ip, y, line);
getSplineInterpolationCoefficients(line, tolerance);
getHessian(line);
putRow(ip, y, line);
stepProgressBar();
}
} /* end getHorizontalHessian */
/*------------------------------------------------------------------*/
public void getVerticalGradient(ImageProcessor ip, double tolerance) {
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
float[] floatPixels = (float[]) ip.getPixels();
int width = ip.getWidth();
int height = ip.getHeight();
double line[] = new double[height];
for (int x = 0; (x < width); x++) {
getColumn(ip, x, line);
getSplineInterpolationCoefficients(line, tolerance);
getGradient(line);
putColumn(ip, x, line);
stepProgressBar();
}
} /* end getVerticalGradient */
/*------------------------------------------------------------------*/
public void getVerticalHessian(ImageProcessor ip, double tolerance) {
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
float[] floatPixels = (float[]) ip.getPixels();
int width = ip.getWidth();
int height = ip.getHeight();
double line[] = new double[height];
for (int x = 0; (x < width); x++) {
getColumn(ip, x, line);
getSplineInterpolationCoefficients(line, tolerance);
getHessian(line);
putColumn(ip, x, line);
stepProgressBar();
}
} /* end getVerticalHessian */
/*------------------------------------------------------------------*/
public void run(String arg) {
ImagePlus imp = WindowManager.getCurrentImage();
this.imp = imp;
if (imp == null) {
IJ.noImage();
return;
}
if ((1 < imp.getStackSize()) && (imp.getType() == imp.COLOR_256)) {
IJ.error("Stack of color images not supported (use grayscale)");
return;
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isRGB()) {
IJ.error("RGB color images not supported (use grayscale)");
return;
}
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isHSB()) {
IJ.error("HSB color images not supported (use grayscale)");
return;
}
}
if (imp.getType() == imp.COLOR_256) {
IJ.error("Indexed color images not supported (use grayscale)");
return;
}
if (imp.getType() == imp.COLOR_RGB) {
IJ.error("Color images not supported (use grayscale)");
return;
}
differentialsDialog dialog =
new differentialsDialog(IJ.getInstance(), "Differentials", true, operation);
GUI.center(dialog);
dialog.setVisible(true);
cancel = dialog.getCancel();
operation = dialog.getOperation();
dialog.dispose();
if (cancel) {
return;
}
imp.startTiming();
if (1 < imp.getStackSize()) {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new StackConverter(imp).convertToGray32();
}
} else {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new ImageConverter(imp).convertToGray32();
}
}
ImageStack stack = imp.getStack();
stackSize = stack.getSize();
Undo.reset();
setupProgressBar();
resetProgressBar();
for (int i = 1; (i <= stackSize); i++) {
ImageProcessor ip = stack.getProcessor(i);
doIt(ip);
imp.getProcessor().resetMinAndMax();
imp.setSlice(i);
imp.updateAndRepaintWindow();
}
imp.getProcessor().resetMinAndMax();
imp.setSlice(1);
imp.updateAndRepaintWindow();
cleanUpProgressBar();
IJ.showTime(imp, imp.getStartTime(), "Differentials: ");
ImageWindow win = imp.getWindow();
if (win != null) {
win.running = false;
}
} /* end run */
/*..................................................................*/
/* Private methods */
/*..................................................................*/
/*------------------------------------------------------------------*/
void antiSymmetricFirMirrorOnBounds(double[] h, double[] c, double[] s) {
if (h.length != 2) {
throw new IndexOutOfBoundsException("The half-length filter size should be 2");
}
if (h[0] != 0.0) {
throw new IllegalArgumentException("Antisymmetry violation (should have h[0]=0.0)");
}
if (c.length != s.length) {
throw new IndexOutOfBoundsException("Incompatible size");
}
if (2 <= c.length) {
s[0] = 0.0;
for (int i = 1; (i < (s.length - 1)); i++) {
s[i] = h[1] * (c[i + 1] - c[i - 1]);
}
s[s.length - 1] = 0.0;
} else {
if (c.length == 1) {
s[0] = 0.0;
} else {
throw new NegativeArraySizeException("Invalid length of data");
}
}
} /* end antiSymmetricFirMirrorOnBounds */
/*------------------------------------------------------------------*/
void cleanUpProgressBar() {
completed = 0;
progressBar.show(2.0);
} /* end cleanUpProgressBar */
/*------------------------------------------------------------------*/
void doIt(ImageProcessor ip) {
int width = ip.getWidth();
int height = ip.getHeight();
double hLine[] = new double[width];
double vLine[] = new double[height];
if (!(ip.getPixels() instanceof float[])) {
throw new IllegalArgumentException("Float image required");
}
switch (operation) {
case GRADIENT_MAGNITUDE:
{
ImageProcessor h = ip.duplicate();
ImageProcessor v = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsH = (float[]) h.getPixels();
float[] floatPixelsV = (float[]) v.getPixels();
getHorizontalGradient(h, FLT_EPSILON);
getVerticalGradient(v, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
Math.sqrt(
floatPixelsH[k] * floatPixelsH[k] + floatPixelsV[k] * floatPixelsV[k]);
}
stepProgressBar();
}
}
break;
case GRADIENT_DIRECTION:
{
ImageProcessor h = ip.duplicate();
ImageProcessor v = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsH = (float[]) h.getPixels();
float[] floatPixelsV = (float[]) v.getPixels();
getHorizontalGradient(h, FLT_EPSILON);
getVerticalGradient(v, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] = (float) Math.atan2(floatPixelsH[k], floatPixelsV[k]);
}
stepProgressBar();
}
}
break;
case LAPLACIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] = (float) (floatPixelsHH[k] + floatPixelsVV[k]);
}
stepProgressBar();
}
}
break;
case LARGEST_HESSIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
(0.5
* (floatPixelsHH[k]
+ floatPixelsVV[k]
+ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
stepProgressBar();
}
}
break;
case SMALLEST_HESSIAN:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
floatPixels[k] =
(float)
(0.5
* (floatPixelsHH[k]
+ floatPixelsVV[k]
- Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
stepProgressBar();
}
}
break;
case HESSIAN_ORIENTATION:
{
ImageProcessor hh = ip.duplicate();
ImageProcessor vv = ip.duplicate();
ImageProcessor hv = ip.duplicate();
float[] floatPixels = (float[]) ip.getPixels();
float[] floatPixelsHH = (float[]) hh.getPixels();
float[] floatPixelsVV = (float[]) vv.getPixels();
float[] floatPixelsHV = (float[]) hv.getPixels();
getHorizontalHessian(hh, FLT_EPSILON);
getVerticalHessian(vv, FLT_EPSILON);
getCrossHessian(hv, FLT_EPSILON);
for (int y = 0, k = 0; (y < height); y++) {
for (int x = 0; (x < width); x++, k++) {
if (floatPixelsHV[k] < 0.0) {
floatPixels[k] =
(float)
(-0.5
* Math.acos(
(floatPixelsHH[k] - floatPixelsVV[k])
/ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
} else {
floatPixels[k] =
(float)
(0.5
* Math.acos(
(floatPixelsHH[k] - floatPixelsVV[k])
/ Math.sqrt(
4.0 * floatPixelsHV[k] * floatPixelsHV[k]
+ (floatPixelsHH[k] - floatPixelsVV[k])
* (floatPixelsHH[k] - floatPixelsVV[k]))));
}
}
stepProgressBar();
}
}
break;
default:
throw new IllegalArgumentException("Invalid operation");
}
ip.resetMinAndMax();
imp.updateAndDraw();
} /* end doIt */
/*------------------------------------------------------------------*/
void getColumn(ImageProcessor ip, int x, double[] column) {
int width = ip.getWidth();
if (ip.getHeight() != column.length) {
throw new IndexOutOfBoundsException("Incoherent array sizes");
}
if (ip.getPixels() instanceof float[]) {
float[] floatPixels = (float[]) ip.getPixels();
for (int i = 0; (i < column.length); i++) {
column[i] = (double) floatPixels[x];
x += width;
}
} else {
throw new IllegalArgumentException("Float image required");
}
} /* end getColumn */
/*------------------------------------------------------------------*/
void getGradient(double[] c) {
double h[] = {0.0, -1.0 / 2.0};
double s[] = new double[c.length];
antiSymmetricFirMirrorOnBounds(h, c, s);
System.arraycopy(s, 0, c, 0, s.length);
} /* end getGradient */
/*------------------------------------------------------------------*/
void getHessian(double[] c) {
double h[] = {-2.0, 1.0};
double s[] = new double[c.length];
symmetricFirMirrorOnBounds(h, c, s);
System.arraycopy(s, 0, c, 0, s.length);
} /* end getHessian */
/*------------------------------------------------------------------*/
double getInitialAntiCausalCoefficientMirrorOnBounds(double[] c, double z, double tolerance) {
return ((z * c[c.length - 2] + c[c.length - 1]) * z / (z * z - 1.0));
} /* end getInitialAntiCausalCoefficientMirrorOnBounds */
/*------------------------------------------------------------------*/
double getInitialCausalCoefficientMirrorOnBounds(double[] c, double z, double tolerance) {
double z1 = z, zn = Math.pow(z, c.length - 1);
double sum = c[0] + zn * c[c.length - 1];
int horizon = c.length;
if (0.0 < tolerance) {
horizon = 2 + (int) (Math.log(tolerance) / Math.log(Math.abs(z)));
horizon = (horizon < c.length) ? (horizon) : (c.length);
}
zn = zn * zn;
for (int n = 1; (n < (horizon - 1)); n++) {
zn = zn / z;
sum = sum + (z1 + zn) * c[n];
z1 = z1 * z;
}
return (sum / (1.0 - Math.pow(z, 2 * c.length - 2)));
} /* end getInitialCausalCoefficientMirrorOnBounds */
/*------------------------------------------------------------------*/
void getRow(ImageProcessor ip, int y, double[] row) {
int rowLength = ip.getWidth();
if (rowLength != row.length) {
throw new IndexOutOfBoundsException("Incoherent array sizes");
}
y *= rowLength;
if (ip.getPixels() instanceof float[]) {
float[] floatPixels = (float[]) ip.getPixels();
for (int i = 0; (i < rowLength); i++) {
row[i] = (double) floatPixels[y++];
}
} else {
throw new IllegalArgumentException("Float image required");
}
} /* end getRow */
/*------------------------------------------------------------------*/
void getSplineInterpolationCoefficients(double[] c, double tolerance) {
double z[] = {Math.sqrt(3.0) - 2.0};
double lambda = 1.0;
if (c.length == 1) {
return;
}
for (int k = 0; (k < z.length); k++) {
lambda = lambda * (1.0 - z[k]) * (1.0 - 1.0 / z[k]);
}
for (int n = 0; (n < c.length); n++) {
c[n] = c[n] * lambda;
}
for (int k = 0; (k < z.length); k++) {
c[0] = getInitialCausalCoefficientMirrorOnBounds(c, z[k], tolerance);
for (int n = 1; (n < c.length); n++) {
c[n] = c[n] + z[k] * c[n - 1];
}
c[c.length - 1] = getInitialAntiCausalCoefficientMirrorOnBounds(c, z[k], tolerance);
for (int n = c.length - 2; (0 <= n); n--) {
c[n] = z[k] * (c[n + 1] - c[n]);
}
}
} /* end getSplineInterpolationCoefficients */
/*------------------------------------------------------------------*/
void putColumn(ImageProcessor ip, int x, double[] column) {
int width = ip.getWidth();
if (ip.getHeight() != column.length) {
throw new IndexOutOfBoundsException("Incoherent array sizes");
}
if (ip.getPixels() instanceof float[]) {
float[] floatPixels = (float[]) ip.getPixels();
for (int i = 0; (i < column.length); i++) {
floatPixels[x] = (float) column[i];
x += width;
}
} else {
throw new IllegalArgumentException("Float image required");
}
} /* end putColumn */
/*------------------------------------------------------------------*/
void putRow(ImageProcessor ip, int y, double[] row) {
int rowLength = ip.getWidth();
if (rowLength != row.length) {
throw new IndexOutOfBoundsException("Incoherent array sizes");
}
y *= rowLength;
if (ip.getPixels() instanceof float[]) {
float[] floatPixels = (float[]) ip.getPixels();
for (int i = 0; (i < rowLength); i++) {
floatPixels[y++] = (float) row[i];
}
} else {
throw new IllegalArgumentException("Float image required");
}
} /* end putRow */
/*------------------------------------------------------------------*/
void resetProgressBar() {
completed = 0;
lastTime = System.currentTimeMillis();
progressBar.show(2.0);
} /* end resetProgressBar */
/*------------------------------------------------------------------*/
void setupProgressBar() {
int height = imp.getHeight();
int width = imp.getWidth();
completed = 0;
lastTime = System.currentTimeMillis();
switch (operation) {
case GRADIENT_MAGNITUDE:
processDuration = stackSize * (width + 2 * height);
break;
case GRADIENT_DIRECTION:
processDuration = stackSize * (width + 2 * height);
break;
case LAPLACIAN:
processDuration = stackSize * (width + 2 * height);
break;
case LARGEST_HESSIAN:
processDuration = stackSize * (2 * width + 3 * height);
break;
case SMALLEST_HESSIAN:
processDuration = stackSize * (2 * width + 3 * height);
break;
case HESSIAN_ORIENTATION:
processDuration = stackSize * (2 * width + 3 * height);
break;
default:
throw new IllegalArgumentException("Invalid operation");
}
} /* end setupProgressBar */
/*------------------------------------------------------------------*/
void stepProgressBar() {
long timeStamp = System.currentTimeMillis();
completed = completed + 1;
if (50L < (timeStamp - lastTime)) {
lastTime = timeStamp;
progressBar.show((double) completed / (double) processDuration);
}
} /* end stepProgressBar */
/*------------------------------------------------------------------*/
void symmetricFirMirrorOnBounds(double[] h, double[] c, double[] s) {
if (h.length != 2) {
throw new IndexOutOfBoundsException("The half-length filter size should be 2");
}
if (c.length != s.length) {
throw new IndexOutOfBoundsException("Incompatible size");
}
if (2 <= c.length) {
s[0] = h[0] * c[0] + 2.0 * h[1] * c[1];
for (int i = 1; (i < (s.length - 1)); i++) {
s[i] = h[0] * c[i] + h[1] * (c[i - 1] + c[i + 1]);
}
s[s.length - 1] = h[0] * c[c.length - 1] + 2.0 * h[1] * c[c.length - 2];
} else {
switch (c.length) {
case 1:
s[0] = (h[0] + 2.0 * h[1]) * c[0];
break;
default:
throw new NegativeArraySizeException("Invalid length of data");
}
}
} /* end symmetricFirMirrorOnBounds */
} /* end class Differentials_ */
/*------------------------------------------------------------------*/
public void run(String arg) {
ImagePlus imp = WindowManager.getCurrentImage();
this.imp = imp;
if (imp == null) {
IJ.noImage();
return;
}
if ((1 < imp.getStackSize()) && (imp.getType() == imp.COLOR_256)) {
IJ.error("Stack of color images not supported (use grayscale)");
return;
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isRGB()) {
IJ.error("RGB color images not supported (use grayscale)");
return;
}
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isHSB()) {
IJ.error("HSB color images not supported (use grayscale)");
return;
}
}
if (imp.getType() == imp.COLOR_256) {
IJ.error("Indexed color images not supported (use grayscale)");
return;
}
if (imp.getType() == imp.COLOR_RGB) {
IJ.error("Color images not supported (use grayscale)");
return;
}
differentialsDialog dialog =
new differentialsDialog(IJ.getInstance(), "Differentials", true, operation);
GUI.center(dialog);
dialog.setVisible(true);
cancel = dialog.getCancel();
operation = dialog.getOperation();
dialog.dispose();
if (cancel) {
return;
}
imp.startTiming();
if (1 < imp.getStackSize()) {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new StackConverter(imp).convertToGray32();
}
} else {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new ImageConverter(imp).convertToGray32();
}
}
ImageStack stack = imp.getStack();
stackSize = stack.getSize();
Undo.reset();
setupProgressBar();
resetProgressBar();
for (int i = 1; (i <= stackSize); i++) {
ImageProcessor ip = stack.getProcessor(i);
doIt(ip);
imp.getProcessor().resetMinAndMax();
imp.setSlice(i);
imp.updateAndRepaintWindow();
}
imp.getProcessor().resetMinAndMax();
imp.setSlice(1);
imp.updateAndRepaintWindow();
cleanUpProgressBar();
IJ.showTime(imp, imp.getStartTime(), "Differentials: ");
ImageWindow win = imp.getWindow();
if (win != null) {
win.running = false;
}
} /* end run */