public void run(String arg) {
ImagePlus imp = WindowManager.getCurrentImage();
if (imp == null) {
IJ.noImage();
return;
}
ImageStack stack1 = imp.getStack();
String fileName = imp.getTitle();
int endslice = stack1.getSize();
ImagePlus imp2 = duplicateStack(imp);
imp2.show();
String duplicateName = imp2.getTitle();
// IJ.showMessage("Box",fileName);
ImageStack stack2 = imp2.getStack();
stack1.deleteSlice(1);
stack2.deleteSlice(endslice);
String calculatorstring =
("image1='"
+ fileName
+ "' operation=Subtract image2="
+ imp2.getTitle()
+ " create stack");
IJ.run("Image Calculator...", calculatorstring);
ImagePlus imp3 = WindowManager.getCurrentImage();
imp3.setTitle(fileName + " DeltaF up");
imp2.getWindow().close();
imp.getWindow().close();
}
/**
* Zooms out by making the source rectangle (srcRect) larger and centering it on (x,y). If we
* can't make it larger, then make the window smaller.
*/
public void zoomOut(int x, int y) {
if (magnification <= 0.03125) return;
double oldMag = magnification;
double newMag = getLowerZoomLevel(magnification);
double srcRatio = (double) srcRect.width / srcRect.height;
double imageRatio = (double) imageWidth / imageHeight;
double initialMag = imp.getWindow().getInitialMagnification();
if (Math.abs(srcRatio - imageRatio) > 0.05) {
double scale = oldMag / newMag;
int newSrcWidth = (int) Math.round(srcRect.width * scale);
int newSrcHeight = (int) Math.round(srcRect.height * scale);
if (newSrcWidth > imageWidth) newSrcWidth = imageWidth;
if (newSrcHeight > imageHeight) newSrcHeight = imageHeight;
int newSrcX = srcRect.x - (newSrcWidth - srcRect.width) / 2;
int newSrcY = srcRect.y - (newSrcHeight - srcRect.height) / 2;
if (newSrcX < 0) newSrcX = 0;
if (newSrcY < 0) newSrcY = 0;
srcRect = new Rectangle(newSrcX, newSrcY, newSrcWidth, newSrcHeight);
// IJ.log(newMag+" "+srcRect+" "+dstWidth+" "+dstHeight);
int newDstWidth = (int) (srcRect.width * newMag);
int newDstHeight = (int) (srcRect.height * newMag);
setMagnification(newMag);
setMaxBounds();
// IJ.log(newDstWidth+" "+dstWidth+" "+newDstHeight+" "+dstHeight);
if (newDstWidth < dstWidth || newDstHeight < dstHeight) {
// IJ.log("pack");
setDrawingSize(newDstWidth, newDstHeight);
imp.getWindow().pack();
} else repaint();
return;
}
if (imageWidth * newMag > dstWidth) {
int w = (int) Math.round(dstWidth / newMag);
if (w * newMag < dstWidth) w++;
int h = (int) Math.round(dstHeight / newMag);
if (h * newMag < dstHeight) h++;
x = offScreenX(x);
y = offScreenY(y);
Rectangle r = new Rectangle(x - w / 2, y - h / 2, w, h);
if (r.x < 0) r.x = 0;
if (r.y < 0) r.y = 0;
if (r.x + w > imageWidth) r.x = imageWidth - w;
if (r.y + h > imageHeight) r.y = imageHeight - h;
srcRect = r;
} else {
srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
setDrawingSize((int) (imageWidth * newMag), (int) (imageHeight * newMag));
// setDrawingSize(dstWidth/2, dstHeight/2);
imp.getWindow().pack();
}
// IJ.write(newMag + " " + srcRect.x+" "+srcRect.y+" "+srcRect.width+" "+srcRect.height+"
// "+dstWidth + " " + dstHeight);
setMagnification(newMag);
// IJ.write(srcRect.x + " " + srcRect.width + " " + dstWidth);
setMaxBounds();
repaint();
}
/** Implements the Image/Zoom/Original Scale command. */
public void unzoom() {
double imag = imp.getWindow().getInitialMagnification();
if (magnification == imag) return;
srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
ImageWindow win = imp.getWindow();
setDrawingSize((int) (imageWidth * imag), (int) (imageHeight * imag));
setMagnification(imag);
setMaxBounds();
win.pack();
setMaxBounds();
repaint();
}
/** Enlarge the canvas if the user enlarges the window. */
void resizeCanvas(int width, int height) {
ImageWindow win = imp.getWindow();
// IJ.log("resizeCanvas: "+srcRect+" "+imageWidth+" "+imageHeight+" "+width+" "+height+"
// "+dstWidth+" "+dstHeight+" "+win.maxBounds);
if (!maxBoundsReset
&& (width > dstWidth || height > dstHeight)
&& win != null
&& win.maxBounds != null
&& width != win.maxBounds.width - 10) {
if (resetMaxBoundsCount != 0)
resetMaxBounds(); // Works around problem that prevented window from being larger than
// maximized size
resetMaxBoundsCount++;
}
if (IJ.altKeyDown()) {
fitToWindow();
return;
}
if (srcRect.width < imageWidth || srcRect.height < imageHeight) {
if (width > imageWidth * magnification) width = (int) (imageWidth * magnification);
if (height > imageHeight * magnification) height = (int) (imageHeight * magnification);
setDrawingSize(width, height);
srcRect.width = (int) (dstWidth / magnification);
srcRect.height = (int) (dstHeight / magnification);
if ((srcRect.x + srcRect.width) > imageWidth) srcRect.x = imageWidth - srcRect.width;
if ((srcRect.y + srcRect.height) > imageHeight) srcRect.y = imageHeight - srcRect.height;
repaint();
}
// IJ.log("resizeCanvas2: "+srcRect+" "+dstWidth+" "+dstHeight+" "+width+" "+height);
}
/** Sets the cursor based on the current tool and cursor location. */
public void setCursor(int sx, int sy, int ox, int oy) {
xMouse = ox;
yMouse = oy;
mouseExited = false;
Roi roi = imp.getRoi();
ImageWindow win = imp.getWindow();
if (win == null) return;
if (IJ.spaceBarDown()) {
setCursor(handCursor);
return;
}
int id = Toolbar.getToolId();
switch (Toolbar.getToolId()) {
case Toolbar.MAGNIFIER:
setCursor(moveCursor);
break;
case Toolbar.HAND:
setCursor(handCursor);
break;
default: // selection tool
if (id == Toolbar.SPARE1 || id >= Toolbar.SPARE2) {
if (Prefs.usePointerCursor) setCursor(defaultCursor);
else setCursor(crosshairCursor);
} else if (roi != null && roi.getState() != roi.CONSTRUCTING && roi.isHandle(sx, sy) >= 0)
setCursor(handCursor);
else if (Prefs.usePointerCursor
|| (roi != null && roi.getState() != roi.CONSTRUCTING && roi.contains(ox, oy)))
setCursor(defaultCursor);
else setCursor(crosshairCursor);
}
}
public void mouseMoved(MouseEvent e) {
// if (ij==null) return;
int sx = e.getX();
int sy = e.getY();
int ox = offScreenX(sx);
int oy = offScreenY(sy);
flags = e.getModifiers();
setCursor(sx, sy, ox, oy);
IJ.setInputEvent(e);
Roi roi = imp.getRoi();
if (roi != null
&& (roi.getType() == Roi.POLYGON
|| roi.getType() == Roi.POLYLINE
|| roi.getType() == Roi.ANGLE)
&& roi.getState() == roi.CONSTRUCTING) {
PolygonRoi pRoi = (PolygonRoi) roi;
pRoi.handleMouseMove(ox, oy);
} else {
if (ox < imageWidth && oy < imageHeight) {
ImageWindow win = imp.getWindow();
// Cursor must move at least 12 pixels before text
// displayed using IJ.showStatus() is overwritten.
if ((sx - sx2) * (sx - sx2) + (sy - sy2) * (sy - sy2) > 144) showCursorStatus = true;
if (win != null && showCursorStatus) win.mouseMoved(ox, oy);
} else IJ.showStatus("");
}
}
void resetMaxBounds() {
ImageWindow win = imp.getWindow();
if (win != null && (System.currentTimeMillis() - win.setMaxBoundsTime) > 500L) {
win.setMaximizedBounds(win.maxWindowBounds);
maxBoundsReset = true;
}
}
void showDialog() {
int width = imp.getWidth();
int height = imp.getHeight();
Calibration cal = imp.getCalibration();
int places;
if (cal.scaled()) {
pixelWidth = cal.pixelWidth;
pixelHeight = cal.pixelHeight;
units = cal.getUnits();
places = 2;
} else {
pixelWidth = 1.0;
pixelHeight = 1.0;
units = "pixels";
places = 0;
}
if (areaPerPoint == 0.0)
areaPerPoint =
(width * cal.pixelWidth * height * cal.pixelHeight) / 81.0; // default to 9x9 grid
ImageWindow win = imp.getWindow();
GenericDialog gd = new GenericDialog("Grid...");
gd.addChoice("Grid Type:", types, type);
gd.addNumericField("Area per Point:", areaPerPoint, places, 6, units + "^2");
gd.addChoice("Color:", colors, color);
gd.addCheckbox("Random Offset", randomOffset);
gd.addDialogListener(this);
gd.showDialog();
if (gd.wasCanceled()) showGrid(null);
}
public void mouseExited(MouseEvent e) {
// autoScroll(e);
ImageWindow win = imp.getWindow();
if (win != null) setCursor(defaultCursor);
IJ.showStatus("");
mouseExited = true;
}
public void run(String arg) {
ImagePlus imp = WindowManager.getImage(id);
if (imp != null && imp.getWindow() != null) {
imp.getWindow().toFront();
return;
}
int colorWidth = 22;
int colorHeight = 16;
int columns = 5;
int rows = 20;
int width = columns * colorWidth;
int height = rows * colorHeight;
ColorGenerator cg = new ColorGenerator(width, height, new int[width * height], this);
cg.drawColors(colorWidth, colorHeight, columns, rows);
setProcessor("CP", cg);
id = getID();
show();
IJ.register(ColorPicker.class);
}
void setMaxBounds() {
if (maxBoundsReset) {
maxBoundsReset = false;
ImageWindow win = imp.getWindow();
if (win != null && !IJ.isLinux() && win.maxBounds != null) {
win.setMaximizedBounds(win.maxBounds);
win.setMaxBoundsTime = System.currentTimeMillis();
}
}
}
void abortPluginOrMacro(ImagePlus imp) {
if (imp != null) {
ImageWindow win = imp.getWindow();
if (win != null) {
win.running = false;
win.running2 = false;
}
}
Macro.abort();
Interpreter.abort();
if (Interpreter.getInstance() != null) IJ.beep();
}
private boolean ignoreArrowKeys(ImagePlus imp) {
Frame frame = WindowManager.getFrontWindow();
String title = frame.getTitle();
if (title != null && title.equals("ROI Manager")) return true;
// Control Panel?
if (frame != null && frame instanceof javax.swing.JFrame) return true;
ImageWindow win = imp.getWindow();
// LOCI Data Browser window?
if (imp.getStackSize() > 1 && win != null && win.getClass().getName().startsWith("loci"))
return true;
return false;
}
public void fitToWindow() {
ImageWindow win = imp.getWindow();
if (win == null) return;
Rectangle bounds = win.getBounds();
Insets insets = win.getInsets();
int sliderHeight = (win instanceof StackWindow) ? 20 : 0;
double xmag = (double) (bounds.width - 10) / srcRect.width;
double ymag = (double) (bounds.height - (10 + insets.top + sliderHeight)) / srcRect.height;
setMagnification(Math.min(xmag, ymag));
int width = (int) (imageWidth * magnification);
int height = (int) (imageHeight * magnification);
if (width == dstWidth && height == dstHeight) return;
srcRect = new Rectangle(0, 0, imageWidth, imageHeight);
setDrawingSize(width, height);
getParent().doLayout();
}
/**
* Zooms in by making the window bigger. If it can't be made bigger, then make the source
* rectangle (srcRect) smaller and center it at (sx,sy). Note that sx and sy are screen
* coordinates.
*/
public void zoomIn(int sx, int sy) {
if (magnification >= 32) return;
double newMag = getHigherZoomLevel(magnification);
int newWidth = (int) (imageWidth * newMag);
int newHeight = (int) (imageHeight * newMag);
Dimension newSize = canEnlarge(newWidth, newHeight);
if (newSize != null) {
setDrawingSize(newSize.width, newSize.height);
if (newSize.width != newWidth || newSize.height != newHeight)
adjustSourceRect(newMag, sx, sy);
else setMagnification(newMag);
imp.getWindow().pack();
} else adjustSourceRect(newMag, sx, sy);
repaint();
if (srcRect.width < imageWidth || srcRect.height < imageHeight) resetMaxBounds();
}
protected void registerActiveImage() {
ImagePlus activeImage = WindowManager.getCurrentImage();
if (activeImage != null
&& activeImage.getProcessor() != null) { // && activeImage.getImageStackSize() > 1
if (currentImage != null && currentImage.getWindow() != null && currentImage != activeImage) {
// System.out.println("remove listener:"+currentImage.getTitle());
ImageUtils.removeScrollListener(currentImage, this, this);
currentImage.killRoi();
currentImage.updateAndDraw();
currentImage = null;
}
if (currentImage != activeImage) {
// System.out.println("add listener:"+activeImage.getTitle());
ImageUtils.addScrollListener(activeImage, this, this);
this.currentImage = activeImage;
}
}
}
/** Implements the Image/Zoom/View 100% command. */
public void zoom100Percent() {
if (magnification == 1.0) return;
double imag = imp.getWindow().getInitialMagnification();
if (magnification != imag) unzoom();
if (magnification == 1.0) return;
if (magnification < 1.0) {
while (magnification < 1.0) zoomIn(imageWidth / 2, imageHeight / 2);
} else if (magnification > 1.0) {
while (magnification > 1.0) zoomOut(imageWidth / 2, imageHeight / 2);
} else return;
int x = xMouse, y = yMouse;
if (mouseExited) {
x = imageWidth / 2;
y = imageHeight / 2;
}
int sx = screenX(x);
int sy = screenY(y);
adjustSourceRect(1.0, sx, sy);
repaint();
}
protected Dimension canEnlarge(int newWidth, int newHeight) {
// if ((flags&Event.CTRL_MASK)!=0 || IJ.controlKeyDown()) return null;
ImageWindow win = imp.getWindow();
if (win == null) return null;
Rectangle r1 = win.getBounds();
Insets insets = win.getInsets();
Point loc = getLocation();
if (loc.x > insets.left + 5 || loc.y > insets.top + 5) {
r1.width = newWidth + insets.left + insets.right + 10;
r1.height = newHeight + insets.top + insets.bottom + 10;
if (win instanceof StackWindow) r1.height += 20;
} else {
r1.width = r1.width - dstWidth + newWidth + 10;
r1.height = r1.height - dstHeight + newHeight + 10;
}
Rectangle max = win.getMaxWindow(r1.x, r1.y);
boolean fitsHorizontally = r1.x + r1.width < max.x + max.width;
boolean fitsVertically = r1.y + r1.height < max.y + max.height;
if (fitsHorizontally && fitsVertically) return new Dimension(newWidth, newHeight);
else if (fitsVertically && newHeight < dstWidth) return new Dimension(dstWidth, newHeight);
else if (fitsHorizontally && newWidth < dstHeight) return new Dimension(newWidth, dstHeight);
else return null;
}
/*------------------------------------------------------------------*/
public void run(String arg) {
ImagePlus imp = WindowManager.getCurrentImage();
this.imp = imp;
if (imp == null) {
IJ.noImage();
return;
}
if ((1 < imp.getStackSize()) && (imp.getType() == imp.COLOR_256)) {
IJ.error("Stack of color images not supported (use grayscale)");
return;
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isRGB()) {
IJ.error("RGB color images not supported (use grayscale)");
return;
}
}
if (1 < imp.getStackSize()) {
if (imp.getStack().isHSB()) {
IJ.error("HSB color images not supported (use grayscale)");
return;
}
}
if (imp.getType() == imp.COLOR_256) {
IJ.error("Indexed color images not supported (use grayscale)");
return;
}
if (imp.getType() == imp.COLOR_RGB) {
IJ.error("Color images not supported (use grayscale)");
return;
}
differentialsDialog dialog =
new differentialsDialog(IJ.getInstance(), "Differentials", true, operation);
GUI.center(dialog);
dialog.setVisible(true);
cancel = dialog.getCancel();
operation = dialog.getOperation();
dialog.dispose();
if (cancel) {
return;
}
imp.startTiming();
if (1 < imp.getStackSize()) {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new StackConverter(imp).convertToGray32();
}
} else {
if (!(imp.getProcessor().getPixels() instanceof float[])) {
new ImageConverter(imp).convertToGray32();
}
}
ImageStack stack = imp.getStack();
stackSize = stack.getSize();
Undo.reset();
setupProgressBar();
resetProgressBar();
for (int i = 1; (i <= stackSize); i++) {
ImageProcessor ip = stack.getProcessor(i);
doIt(ip);
imp.getProcessor().resetMinAndMax();
imp.setSlice(i);
imp.updateAndRepaintWindow();
}
imp.getProcessor().resetMinAndMax();
imp.setSlice(1);
imp.updateAndRepaintWindow();
cleanUpProgressBar();
IJ.showTime(imp, imp.getStartTime(), "Differentials: ");
ImageWindow win = imp.getWindow();
if (win != null) {
win.running = false;
}
} /* end run */
String getInfo(ImagePlus imp, ImageProcessor ip) {
String s = new String("\n");
s += "Title: " + imp.getTitle() + "\n";
Calibration cal = imp.getCalibration();
int stackSize = imp.getStackSize();
int channels = imp.getNChannels();
int slices = imp.getNSlices();
int frames = imp.getNFrames();
int digits = imp.getBitDepth() == 32 ? 4 : 0;
if (cal.scaled()) {
String unit = cal.getUnit();
String units = cal.getUnits();
s +=
"Width: "
+ IJ.d2s(imp.getWidth() * cal.pixelWidth, 2)
+ " "
+ units
+ " ("
+ imp.getWidth()
+ ")\n";
s +=
"Height: "
+ IJ.d2s(imp.getHeight() * cal.pixelHeight, 2)
+ " "
+ units
+ " ("
+ imp.getHeight()
+ ")\n";
if (slices > 1)
s += "Depth: " + IJ.d2s(slices * cal.pixelDepth, 2) + " " + units + " (" + slices + ")\n";
double xResolution = 1.0 / cal.pixelWidth;
double yResolution = 1.0 / cal.pixelHeight;
int places = Tools.getDecimalPlaces(xResolution, yResolution);
if (xResolution == yResolution)
s += "Resolution: " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n";
else {
s += "X Resolution: " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n";
s += "Y Resolution: " + IJ.d2s(yResolution, places) + " pixels per " + unit + "\n";
}
} else {
s += "Width: " + imp.getWidth() + " pixels\n";
s += "Height: " + imp.getHeight() + " pixels\n";
if (stackSize > 1) s += "Depth: " + slices + " pixels\n";
}
if (stackSize > 1)
s +=
"Voxel size: "
+ d2s(cal.pixelWidth)
+ "x"
+ d2s(cal.pixelHeight)
+ "x"
+ d2s(cal.pixelDepth)
+ " "
+ cal.getUnit()
+ "\n";
else
s +=
"Pixel size: "
+ d2s(cal.pixelWidth)
+ "x"
+ d2s(cal.pixelHeight)
+ " "
+ cal.getUnit()
+ "\n";
s += "ID: " + imp.getID() + "\n";
String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : "";
s += "Coordinate origin: " + d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin + "\n";
int type = imp.getType();
switch (type) {
case ImagePlus.GRAY8:
s += "Bits per pixel: 8 ";
String lut = "LUT";
if (imp.getProcessor().isColorLut()) lut = "color " + lut;
else lut = "grayscale " + lut;
if (imp.isInvertedLut()) lut = "inverting " + lut;
s += "(" + lut + ")\n";
if (imp.getNChannels() > 1) s += displayRanges(imp);
else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n";
break;
case ImagePlus.GRAY16:
case ImagePlus.GRAY32:
if (type == ImagePlus.GRAY16) {
String sign = cal.isSigned16Bit() ? "signed" : "unsigned";
s += "Bits per pixel: 16 (" + sign + ")\n";
} else s += "Bits per pixel: 32 (float)\n";
if (imp.getNChannels() > 1) s += displayRanges(imp);
else {
s += "Display range: ";
double min = ip.getMin();
double max = ip.getMax();
if (cal.calibrated()) {
min = cal.getCValue((int) min);
max = cal.getCValue((int) max);
}
s += IJ.d2s(min, digits) + " - " + IJ.d2s(max, digits) + "\n";
}
break;
case ImagePlus.COLOR_256:
s += "Bits per pixel: 8 (color LUT)\n";
break;
case ImagePlus.COLOR_RGB:
s += "Bits per pixel: 32 (RGB)\n";
break;
}
double interval = cal.frameInterval;
double fps = cal.fps;
if (stackSize > 1) {
ImageStack stack = imp.getStack();
int slice = imp.getCurrentSlice();
String number = slice + "/" + stackSize;
String label = stack.getShortSliceLabel(slice);
if (label != null && label.length() > 0) label = " (" + label + ")";
else label = "";
if (interval > 0.0 || fps != 0.0) {
s += "Frame: " + number + label + "\n";
if (fps != 0.0) {
String sRate =
Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5);
s += "Frame rate: " + sRate + " fps\n";
}
if (interval != 0.0)
s +=
"Frame interval: "
+ ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5))
+ " "
+ cal.getTimeUnit()
+ "\n";
} else s += "Image: " + number + label + "\n";
if (imp.isHyperStack()) {
if (channels > 1) s += " Channel: " + imp.getChannel() + "/" + channels + "\n";
if (slices > 1) s += " Slice: " + imp.getSlice() + "/" + slices + "\n";
if (frames > 1) s += " Frame: " + imp.getFrame() + "/" + frames + "\n";
}
if (imp.isComposite()) {
if (!imp.isHyperStack() && channels > 1) s += " Channels: " + channels + "\n";
String mode = ((CompositeImage) imp).getModeAsString();
s += " Composite mode: \"" + mode + "\"\n";
}
}
if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No Threshold\n";
else {
double lower = ip.getMinThreshold();
double upper = ip.getMaxThreshold();
int dp = digits;
if (cal.calibrated()) {
lower = cal.getCValue((int) lower);
upper = cal.getCValue((int) upper);
dp = cal.isSigned16Bit() ? 0 : 4;
}
s += "Threshold: " + IJ.d2s(lower, dp) + "-" + IJ.d2s(upper, dp) + "\n";
}
ImageCanvas ic = imp.getCanvas();
double mag = ic != null ? ic.getMagnification() : 1.0;
if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n";
if (cal.calibrated()) {
s += " \n";
int curveFit = cal.getFunction();
s += "Calibration Function: ";
if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n";
else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n";
else s += CurveFitter.fList[curveFit] + "\n";
double[] c = cal.getCoefficients();
if (c != null) {
s += " a: " + IJ.d2s(c[0], 6) + "\n";
s += " b: " + IJ.d2s(c[1], 6) + "\n";
if (c.length >= 3) s += " c: " + IJ.d2s(c[2], 6) + "\n";
if (c.length >= 4) s += " c: " + IJ.d2s(c[3], 6) + "\n";
if (c.length >= 5) s += " c: " + IJ.d2s(c[4], 6) + "\n";
}
s += " Unit: \"" + cal.getValueUnit() + "\"\n";
} else s += "Uncalibrated\n";
FileInfo fi = imp.getOriginalFileInfo();
if (fi != null) {
if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n";
else if (fi.directory != null && fi.fileName != null)
s += "Path: " + fi.directory + fi.fileName + "\n";
}
ImageWindow win = imp.getWindow();
if (win != null) {
Point loc = win.getLocation();
Dimension screen = IJ.getScreenSize();
s +=
"Screen location: "
+ loc.x
+ ","
+ loc.y
+ " ("
+ screen.width
+ "x"
+ screen.height
+ ")\n";
}
Overlay overlay = imp.getOverlay();
if (overlay != null) {
String hidden = imp.getHideOverlay() ? " (hidden)" : " ";
int n = overlay.size();
String elements = n == 1 ? " element" : " elements";
s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n";
} else s += "No Overlay\n";
Roi roi = imp.getRoi();
if (roi == null) {
if (cal.calibrated()) s += " \n";
s += "No Selection\n";
} else if (roi instanceof EllipseRoi) {
s += "\nElliptical Selection\n";
double[] p = ((EllipseRoi) roi).getParams();
double dx = p[2] - p[0];
double dy = p[3] - p[1];
double major = Math.sqrt(dx * dx + dy * dy);
s += " Major: " + IJ.d2s(major, 2) + "\n";
s += " Minor: " + IJ.d2s(major * p[4], 2) + "\n";
s += " X1: " + IJ.d2s(p[0], 2) + "\n";
s += " Y1: " + IJ.d2s(p[1], 2) + "\n";
s += " X2: " + IJ.d2s(p[2], 2) + "\n";
s += " Y2: " + IJ.d2s(p[3], 2) + "\n";
s += " Aspect ratio: " + IJ.d2s(p[4], 2) + "\n";
} else {
s += " \n";
s += roi.getTypeAsString() + " Selection";
String points = null;
if (roi instanceof PointRoi) {
int npoints = ((PolygonRoi) roi).getNCoordinates();
String suffix = npoints > 1 ? "s)" : ")";
points = " (" + npoints + " point" + suffix;
}
String name = roi.getName();
if (name != null) {
s += " (\"" + name + "\")";
if (points != null) s += "\n " + points;
} else if (points != null) s += points;
s += "\n";
Rectangle r = roi.getBounds();
if (roi instanceof Line) {
Line line = (Line) roi;
s += " X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n";
s += " Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n";
s += " X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n";
s += " Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n";
} else if (cal.scaled()) {
s += " X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n";
s += " Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n";
s += " Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n";
s += " Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n";
} else {
s += " X: " + r.x + "\n";
s += " Y: " + yy(r.y, imp) + "\n";
s += " Width: " + r.width + "\n";
s += " Height: " + r.height + "\n";
}
}
return s;
}
/**
* Performs particle analysis on the specified ImagePlus and ImageProcessor. Returns false if
* there is an error.
*/
public boolean analyze(ImagePlus imp, ImageProcessor ip) {
if (this.imp == null) this.imp = imp;
showResults = (options & SHOW_RESULTS) != 0;
excludeEdgeParticles = (options & EXCLUDE_EDGE_PARTICLES) != 0;
resetCounter = (options & CLEAR_WORKSHEET) != 0;
showProgress = (options & SHOW_PROGRESS) != 0;
floodFill = (options & INCLUDE_HOLES) == 0;
recordStarts = (options & RECORD_STARTS) != 0;
addToManager = (options & ADD_TO_MANAGER) != 0;
displaySummary = (options & DISPLAY_SUMMARY) != 0;
inSituShow = (options & IN_SITU_SHOW) != 0;
outputImage = null;
ip.snapshot();
ip.setProgressBar(null);
if (Analyzer.isRedirectImage()) {
redirectImp = Analyzer.getRedirectImage(imp);
if (redirectImp == null) return false;
int depth = redirectImp.getStackSize();
if (depth > 1 && depth == imp.getStackSize()) {
ImageStack redirectStack = redirectImp.getStack();
redirectIP = redirectStack.getProcessor(imp.getCurrentSlice());
} else redirectIP = redirectImp.getProcessor();
} else if (imp.getType() == ImagePlus.COLOR_RGB) {
ImagePlus original = (ImagePlus) imp.getProperty("OriginalImage");
if (original != null
&& original.getWidth() == imp.getWidth()
&& original.getHeight() == imp.getHeight()) {
redirectImp = original;
redirectIP = original.getProcessor();
}
}
if (!setThresholdLevels(imp, ip)) return false;
width = ip.getWidth();
height = ip.getHeight();
if (!(showChoice == NOTHING || showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS)) {
blackBackground = Prefs.blackBackground && inSituShow;
if (slice == 1) outlines = new ImageStack(width, height);
if (showChoice == ROI_MASKS) drawIP = new ShortProcessor(width, height);
else drawIP = new ByteProcessor(width, height);
drawIP.setLineWidth(lineWidth);
if (showChoice == ROI_MASKS) {
} // Place holder for now...
else if (showChoice == MASKS && !blackBackground) drawIP.invertLut();
else if (showChoice == OUTLINES) {
if (!inSituShow) {
if (customLut == null) makeCustomLut();
drawIP.setColorModel(customLut);
}
drawIP.setFont(new Font("SansSerif", Font.PLAIN, fontSize));
if (fontSize > 12 && inSituShow) drawIP.setAntialiasedText(true);
}
outlines.addSlice(null, drawIP);
if (showChoice == ROI_MASKS || blackBackground) {
drawIP.setColor(Color.black);
drawIP.fill();
drawIP.setColor(Color.white);
} else {
drawIP.setColor(Color.white);
drawIP.fill();
drawIP.setColor(Color.black);
}
}
calibration = redirectImp != null ? redirectImp.getCalibration() : imp.getCalibration();
if (rt == null) {
rt = Analyzer.getResultsTable();
analyzer = new Analyzer(imp);
} else analyzer = new Analyzer(imp, measurements, rt);
if (resetCounter && slice == 1) {
if (!Analyzer.resetCounter()) return false;
}
beginningCount = Analyzer.getCounter();
byte[] pixels = null;
if (ip instanceof ByteProcessor) pixels = (byte[]) ip.getPixels();
if (r == null) {
r = ip.getRoi();
mask = ip.getMask();
if (displaySummary) {
if (mask != null) totalArea = ImageStatistics.getStatistics(ip, AREA, calibration).area;
else totalArea = r.width * calibration.pixelWidth * r.height * calibration.pixelHeight;
}
}
minX = r.x;
maxX = r.x + r.width;
minY = r.y;
maxY = r.y + r.height;
if (r.width < width || r.height < height || mask != null) {
if (!eraseOutsideRoi(ip, r, mask)) return false;
}
int offset;
double value;
int inc = Math.max(r.height / 25, 1);
int mi = 0;
ImageWindow win = imp.getWindow();
if (win != null) win.running = true;
if (measurements == 0) measurements = Analyzer.getMeasurements();
if (showChoice == ELLIPSES) measurements |= ELLIPSE;
measurements &= ~LIMIT; // ignore "Limit to Threshold"
roiNeedsImage =
(measurements & PERIMETER) != 0
|| (measurements & SHAPE_DESCRIPTORS) != 0
|| (measurements & FERET) != 0;
particleCount = 0;
wand = new Wand(ip);
pf = new PolygonFiller();
if (floodFill) {
ImageProcessor ipf = ip.duplicate();
ipf.setValue(fillColor);
ff = new FloodFiller(ipf);
}
roiType = Wand.allPoints() ? Roi.FREEROI : Roi.TRACED_ROI;
for (int y = r.y; y < (r.y + r.height); y++) {
offset = y * width;
for (int x = r.x; x < (r.x + r.width); x++) {
if (pixels != null) value = pixels[offset + x] & 255;
else if (imageType == SHORT) value = ip.getPixel(x, y);
else value = ip.getPixelValue(x, y);
if (value >= level1 && value <= level2) analyzeParticle(x, y, imp, ip);
}
if (showProgress && ((y % inc) == 0)) IJ.showProgress((double) (y - r.y) / r.height);
if (win != null) canceled = !win.running;
if (canceled) {
Macro.abort();
break;
}
}
if (showProgress) IJ.showProgress(1.0);
if (showResults) rt.updateResults();
imp.killRoi();
ip.resetRoi();
ip.reset();
if (displaySummary && IJ.getInstance() != null) updateSliceSummary();
if (addToManager && roiManager != null) roiManager.setEditMode(imp, true);
maxParticleCount = (particleCount > maxParticleCount) ? particleCount : maxParticleCount;
totalCount += particleCount;
if (!canceled) showResults();
return true;
}
public void mousePressed(MouseEvent e) {
// if (ij==null) return;
showCursorStatus = true;
int toolID = Toolbar.getToolId();
ImageWindow win = imp.getWindow();
if (win != null && win.running2 && toolID != Toolbar.MAGNIFIER) {
if (win instanceof StackWindow) ((StackWindow) win).setAnimate(false);
else win.running2 = false;
return;
}
int x = e.getX();
int y = e.getY();
flags = e.getModifiers();
// IJ.log("Mouse pressed: " + e.isPopupTrigger() + " " + ij.modifiers(flags));
// if (toolID!=Toolbar.MAGNIFIER && e.isPopupTrigger()) {
if (toolID != Toolbar.MAGNIFIER
&& (e.isPopupTrigger() || (!IJ.isMacintosh() && (flags & Event.META_MASK) != 0))) {
handlePopupMenu(e);
return;
}
int ox = offScreenX(x);
int oy = offScreenY(y);
xMouse = ox;
yMouse = oy;
if (IJ.spaceBarDown()) {
// temporarily switch to "hand" tool of space bar down
setupScroll(ox, oy);
return;
}
if (showAllROIs) {
Roi roi = imp.getRoi();
if (!(roi != null && (roi.contains(ox, oy) || roi.isHandle(x, y) >= 0))
&& roiManagerSelect(x, y)) return;
}
if (customRoi && overlay != null) return;
switch (toolID) {
case Toolbar.MAGNIFIER:
if (IJ.shiftKeyDown()) zoomToSelection(ox, oy);
else if ((flags & (Event.ALT_MASK | Event.META_MASK | Event.CTRL_MASK)) != 0) {
// IJ.run("Out");
zoomOut(x, y);
if (getMagnification() < 1.0) imp.repaintWindow();
} else {
// IJ.run("In");
zoomIn(x, y);
if (getMagnification() <= 1.0) imp.repaintWindow();
}
break;
case Toolbar.HAND:
setupScroll(ox, oy);
break;
case Toolbar.DROPPER:
setDrawingColor(ox, oy, IJ.altKeyDown());
break;
case Toolbar.WAND:
Roi roi = imp.getRoi();
if (roi != null && roi.contains(ox, oy)) {
Rectangle r = roi.getBounds();
if (r.width == imageWidth && r.height == imageHeight) imp.killRoi();
else if (!e.isAltDown()) {
handleRoiMouseDown(e);
return;
}
}
if (roi != null) {
int handle = roi.isHandle(x, y);
if (handle >= 0) {
roi.mouseDownInHandle(handle, x, y);
return;
}
}
setRoiModState(e, roi, -1);
String mode = WandToolOptions.getMode();
double tolerance = WandToolOptions.getTolerance();
int npoints = IJ.doWand(ox, oy, tolerance, mode);
if (Recorder.record && npoints > 0) {
if (tolerance == 0.0 && mode.equals("Legacy")) Recorder.record("doWand", ox, oy);
else
Recorder.recordString(
"doWand(" + ox + ", " + oy + ", " + tolerance + ", \"" + mode + "\");\n");
}
break;
case Toolbar.OVAL:
if (Toolbar.getBrushSize() > 0) new RoiBrush();
else handleRoiMouseDown(e);
break;
case Toolbar.SPARE1:
case Toolbar.SPARE2:
case Toolbar.SPARE3:
case Toolbar.SPARE4:
case Toolbar.SPARE5:
case Toolbar.SPARE6:
case Toolbar.SPARE7:
case Toolbar.SPARE8:
case Toolbar.SPARE9:
Toolbar.getInstance().runMacroTool(toolID);
break;
default: // selection tool
handleRoiMouseDown(e);
}
}