void skeletonize(ImageProcessor ip) {
if (Prefs.blackBackground) ip.invert();
boolean edgePixels = hasEdgePixels(ip);
ImageProcessor ip2 = expand(ip, edgePixels);
((ByteProcessor) ip2).skeletonize();
ip = shrink(ip, ip2, edgePixels);
if (Prefs.blackBackground) ip.invert();
}
public static void run() {
ImagePlus imgPlus = new ImagePlus("data/in/sample1.tiff");
ImageProcessor imgProcessor = imgPlus.getProcessor();
imgProcessor.invert();
FileSaver fs = new FileSaver(imgPlus);
fs.saveAsJpeg("data/out/sample1-inverted.jpg");
}
boolean eraseOutsideRoi(ImageProcessor ip, Rectangle r, ImageProcessor mask) {
int width = ip.getWidth();
int height = ip.getHeight();
ip.setRoi(r);
if (excludeEdgeParticles && polygon != null) {
ImageStatistics stats = ImageStatistics.getStatistics(ip, MIN_MAX, null);
if (fillColor >= stats.min && fillColor <= stats.max) {
double replaceColor = level1 - 1.0;
if (replaceColor < 0.0 || replaceColor == fillColor) {
replaceColor = level2 + 1.0;
int maxColor = imageType == BYTE ? 255 : 65535;
if (replaceColor > maxColor || replaceColor == fillColor) {
IJ.error("Particle Analyzer", "Unable to remove edge particles");
return false;
}
}
for (int y = minY; y < maxY; y++) {
for (int x = minX; x < maxX; x++) {
int v = ip.getPixel(x, y);
if (v == fillColor) ip.putPixel(x, y, (int) replaceColor);
}
}
}
}
ip.setValue(fillColor);
if (mask != null) {
mask = mask.duplicate();
mask.invert();
ip.fill(mask);
}
ip.setRoi(0, 0, r.x, height);
ip.fill();
ip.setRoi(r.x, 0, r.width, r.y);
ip.fill();
ip.setRoi(r.x, r.y + r.height, r.width, height - (r.y + r.height));
ip.fill();
ip.setRoi(r.x + r.width, 0, width - (r.x + r.width), height);
ip.fill();
ip.resetRoi();
// IJ.log("erase: "+fillColor+" "+level1+" "+level2+" "+excludeEdgeParticles);
// (new ImagePlus("ip2", ip.duplicate())).show();
return true;
}
private void makeBand(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi == null) {
noRoi("Make Band");
return;
}
if (!roi.isArea()) {
IJ.error("Make Band", "Area selection required");
return;
}
Calibration cal = imp.getCalibration();
double pixels = bandSize;
double size = pixels * cal.pixelWidth;
int decimalPlaces = 0;
if ((int) size != size) decimalPlaces = 2;
GenericDialog gd = new GenericDialog("Make Band");
gd.addNumericField("Band Size:", size, decimalPlaces, 4, cal.getUnits());
gd.showDialog();
if (gd.wasCanceled()) return;
size = gd.getNextNumber();
if (Double.isNaN(size)) {
IJ.error("Make Band", "invalid number");
return;
}
int n = (int) Math.round(size / cal.pixelWidth);
if (n > 255) {
IJ.error("Make Band", "Cannot make bands wider that 255 pixels");
return;
}
int width = imp.getWidth();
int height = imp.getHeight();
Rectangle r = roi.getBounds();
ImageProcessor ip = roi.getMask();
if (ip == null) {
ip = new ByteProcessor(r.width, r.height);
ip.invert();
}
ImageProcessor mask = new ByteProcessor(width, height);
mask.insert(ip, r.x, r.y);
ImagePlus edm = new ImagePlus("mask", mask);
boolean saveBlackBackground = Prefs.blackBackground;
Prefs.blackBackground = false;
IJ.run(edm, "Distance Map", "");
Prefs.blackBackground = saveBlackBackground;
ip = edm.getProcessor();
ip.setThreshold(0, n, ImageProcessor.NO_LUT_UPDATE);
int xx = -1, yy = -1;
for (int x = r.x; x < r.x + r.width; x++) {
for (int y = r.y; y < r.y + r.height; y++) {
if (ip.getPixel(x, y) < n) {
xx = x;
yy = y;
break;
}
}
if (xx >= 0 || yy >= 0) break;
}
int count = IJ.doWand(edm, xx, yy, 0, null);
if (count <= 0) {
IJ.error("Make Band", "Unable to make band");
return;
}
ShapeRoi roi2 = new ShapeRoi(edm.getRoi());
if (!(roi instanceof ShapeRoi)) roi = new ShapeRoi(roi);
ShapeRoi roi1 = (ShapeRoi) roi;
roi2 = roi2.not(roi1);
imp.setRoi(roi2);
bandSize = n;
}
void outline(ImageProcessor ip) {
if (Prefs.blackBackground) ip.invert();
((ByteProcessor) ip).outline();
if (Prefs.blackBackground) ip.invert();
}
void apply(ImagePlus imp, ImageProcessor ip) {
// this.setCursor(wait);
// IJ.showStatus("Bandpassing slice "+previousSlice);
java.awt.Color col;
if (invert.getState()) col = Toolbar.getForegroundColor();
else col = Toolbar.getBackgroundColor();
ip.setColor(col);
int fill = ip.BLACK;
int keep = 0;
if (bandPassH.getState() && bandPassS.getState() && bandPassB.getState()) { // PPP All pass
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue < minHue) || (hue > maxHue))
|| ((sat < minSat) || (sat > maxSat))
|| ((bri < minBri) || (bri > maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (!bandPassH.getState()
&& !bandPassS.getState()
&& !bandPassB.getState()) { // SSS All stop
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue >= minHue) && (hue <= maxHue))
|| ((sat >= minSat) && (sat <= maxSat))
|| ((bri >= minBri) && (bri <= maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (bandPassH.getState() && bandPassS.getState() && !bandPassB.getState()) { // PPS
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue < minHue) || (hue > maxHue))
|| ((sat < minSat) || (sat > maxSat))
|| ((bri >= minBri) && (bri <= maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (!bandPassH.getState() && !bandPassS.getState() && bandPassB.getState()) { // SSP
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue >= minHue) && (hue <= maxHue))
|| ((sat >= minSat) && (sat <= maxSat))
|| ((bri < minBri) || (bri > maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (bandPassH.getState() && !bandPassS.getState() && !bandPassB.getState()) { // PSS
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue < minHue) || (hue > maxHue))
|| ((sat >= minSat) && (sat <= maxSat))
|| ((bri >= minBri) && (bri <= maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (!bandPassH.getState() && bandPassS.getState() && bandPassB.getState()) { // SPP
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue >= minHue) && (hue <= maxHue))
|| ((sat < minSat) || (sat > maxSat))
|| ((bri < minBri) || (bri > maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (!bandPassH.getState() && bandPassS.getState() && !bandPassB.getState()) { // SPS
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue >= minHue) && (hue <= maxHue))
|| ((sat < minSat) || (sat > maxSat))
|| ((bri >= minBri) && (bri <= maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
} else if (bandPassH.getState() && !bandPassS.getState() && bandPassB.getState()) { // PSP
for (int j = 0; j < numPixels; j++) {
int hue = hSource[j] & 0xff;
int sat = sSource[j] & 0xff;
int bri = bSource[j] & 0xff;
if (((hue < minHue) || (hue > maxHue))
|| ((sat >= minSat) && (sat <= maxSat))
|| ((bri < minBri) || (bri > maxBri))) fillMask[j] = fill;
else fillMask[j] = keep;
}
}
ip.fill(fillMask);
if (threshold.getState()) {
ip.invert();
for (int j = 0; j < numPixels; j++) {
if (fillMask[j] == fill) fillMask[j] = keep;
else fillMask[j] = fill;
}
ip.fill(fillMask);
ip.invert();
}
}