/**
* Create a common SAMFileWriter from a factory for use with GATK tools. Assumes that if the
* factory has been set to create an index, the header must be set to coordinate sorted.
*
* @param outputFile if this file has a .cram extension then a reference is required. Can not be
* null.
* @param referenceFile the reference source to use. Can not be null if a output file has a .cram
* extension.
* @param header header to be used for the output writer
* @param preSorted if true then records must already be sorted to match the header sort order
* @param factory SAMFileWriterFactory factory to use
* @return SAMFileWriter
*/
public static SAMFileWriter createCommonSAMWriterFromFactory(
final SAMFileWriterFactory factory,
final File outputFile,
final File referenceFile,
final SAMFileHeader header,
final boolean preSorted) {
Utils.nonNull(outputFile);
Utils.nonNull(header);
if (null == referenceFile && outputFile.getName().endsWith(CramIO.CRAM_FILE_EXTENSION)) {
throw new UserException("A reference file is required for writing CRAM files");
}
return factory.makeWriter(header.clone(), preSorted, outputFile, referenceFile);
}
@Override
public int doWork(String[] args) {
boolean compressed = false;
int maxRecordsInRAM = 100000;
long count = -1L;
File fileout = null;
com.github.lindenb.jvarkit.util.cli.GetOpt opt =
new com.github.lindenb.jvarkit.util.cli.GetOpt();
int c;
while ((c = opt.getopt(args, getGetOptDefault() + "o:n:N:T:b")) != -1) {
switch (c) {
case 'b':
compressed = true;
break;
case 'N':
maxRecordsInRAM = Integer.parseInt(opt.getOptArg());
break;
case 'n':
count = Long.parseLong(opt.getOptArg());
break;
case 'o':
fileout = new File(opt.getOptArg());
break;
case 'T':
this.addTmpDirectory(new File(opt.getOptArg()));
break;
default:
{
switch (handleOtherOptions(c, opt, null)) {
case EXIT_FAILURE:
return -1;
case EXIT_SUCCESS:
return 0;
default:
break;
}
}
}
}
if (count < -1L) // -1 == infinite
{
error("Bad count:" + count);
return -1;
}
SamReader samReader = null;
SAMRecordIterator iter = null;
SAMFileWriter samWriter = null;
Random random = new Random();
CloseableIterator<RandSamRecord> iter2 = null;
try {
SamFileReaderFactory.setDefaultValidationStringency(ValidationStringency.SILENT);
if (opt.getOptInd() == args.length) {
info("Reading from stdin");
samReader = SamFileReaderFactory.mewInstance().openStdin();
} else if (opt.getOptInd() + 1 == args.length) {
File filename = new File(args[opt.getOptInd()]);
info("Reading from " + filename);
samReader = SamFileReaderFactory.mewInstance().open(filename);
} else {
error("Illegal number of arguments.");
return -1;
}
SAMFileHeader header = samReader.getFileHeader();
header = header.clone();
header.setSortOrder(SortOrder.unsorted);
header.addComment("Processed with " + getProgramName() + " : " + getProgramCommandLine());
SAMFileWriterFactory sfw = new SAMFileWriterFactory();
sfw.setCreateIndex(false);
sfw.setCreateMd5File(false);
if (fileout == null) {
if (compressed) {
samWriter = sfw.makeBAMWriter(header, true, System.out);
} else {
samWriter = sfw.makeSAMWriter(header, true, System.out);
}
} else {
samWriter = sfw.makeSAMOrBAMWriter(header, true, fileout);
this.addTmpDirectory(fileout);
}
iter = samReader.iterator();
SAMSequenceDictionaryProgress progress =
new SAMSequenceDictionaryProgress(samReader.getFileHeader().getSequenceDictionary());
SortingCollection<RandSamRecord> sorter =
SortingCollection.newInstance(
RandSamRecord.class,
new RandSamRecordCodec(header),
new RandSamRecordComparator(),
maxRecordsInRAM,
getTmpDirectories());
sorter.setDestructiveIteration(true);
while (iter.hasNext()) {
RandSamRecord r = new RandSamRecord();
r.rand_index = random.nextInt();
r.samRecord = progress.watch(iter.next());
sorter.add(r);
}
iter.close();
iter = null;
sorter.doneAdding();
iter2 = sorter.iterator();
if (count == -1) {
while (iter2.hasNext()) {
samWriter.addAlignment(iter2.next().samRecord);
}
} else {
while (iter2.hasNext() && count > 0) {
samWriter.addAlignment(iter2.next().samRecord);
count--;
}
}
iter2.close();
iter2 = null;
sorter.cleanup();
progress.finish();
} catch (Exception e) {
error(e);
return -1;
} finally {
CloserUtil.close(iter);
CloserUtil.close(iter2);
CloserUtil.close(samReader);
CloserUtil.close(samWriter);
}
return 0;
}
/**
* HACK: This is used to make a copy of a header. Really, SAMFileHeader should provide a copy
* constructor or a factory method.
*/
public static SAMFileHeader cloneSAMFileHeader(final SAMFileHeader header) {
if (header == null) return null;
return header.clone();
}