void analyzeParticle(int x, int y, ImagePlus imp, ImageProcessor ip) { // Wand wand = new Wand(ip); ImageProcessor ip2 = redirectIP != null ? redirectIP : ip; wand.autoOutline(x, y, level1, level2, wandMode); if (wand.npoints == 0) { IJ.log("wand error: " + x + " " + y); return; } Roi roi = new PolygonRoi(wand.xpoints, wand.ypoints, wand.npoints, roiType); Rectangle r = roi.getBounds(); if (r.width > 1 && r.height > 1) { PolygonRoi proi = (PolygonRoi) roi; pf.setPolygon(proi.getXCoordinates(), proi.getYCoordinates(), proi.getNCoordinates()); ip2.setMask(pf.getMask(r.width, r.height)); if (floodFill) ff.particleAnalyzerFill(x, y, level1, level2, ip2.getMask(), r); } ip2.setRoi(r); ip.setValue(fillColor); ImageStatistics stats = getStatistics(ip2, measurements, calibration); boolean include = true; if (excludeEdgeParticles) { if (r.x == minX || r.y == minY || r.x + r.width == maxX || r.y + r.height == maxY) include = false; if (polygon != null) { Rectangle bounds = roi.getBounds(); int x1 = bounds.x + wand.xpoints[wand.npoints - 1]; int y1 = bounds.y + wand.ypoints[wand.npoints - 1]; int x2, y2; for (int i = 0; i < wand.npoints; i++) { x2 = bounds.x + wand.xpoints[i]; y2 = bounds.y + wand.ypoints[i]; if (!polygon.contains(x2, y2)) { include = false; break; } if ((x1 == x2 && ip.getPixel(x1, y1 - 1) == fillColor) || (y1 == y2 && ip.getPixel(x1 - 1, y1) == fillColor)) { include = false; break; } x1 = x2; y1 = y2; } } } ImageProcessor mask = ip2.getMask(); if (minCircularity > 0.0 || maxCircularity < 1.0) { double perimeter = roi.getLength(); double circularity = perimeter == 0.0 ? 0.0 : 4.0 * Math.PI * (stats.pixelCount / (perimeter * perimeter)); if (circularity > 1.0) circularity = 1.0; // IJ.log(circularity+" "+perimeter+" "+stats.area); if (circularity < minCircularity || circularity > maxCircularity) include = false; } if (stats.pixelCount >= minSize && stats.pixelCount <= maxSize && include) { particleCount++; if (roiNeedsImage) roi.setImage(imp); stats.xstart = x; stats.ystart = y; saveResults(stats, roi); if (showChoice != NOTHING) drawParticle(drawIP, roi, stats, mask); } if (redirectIP != null) ip.setRoi(r); ip.fill(mask); }