Example #1
0
 void abortPluginOrMacro(ImagePlus imp) {
   if (imp != null) {
     ImageWindow win = imp.getWindow();
     if (win != null) {
       win.running = false;
       win.running2 = false;
     }
   }
   Macro.abort();
   Interpreter.abort();
   if (Interpreter.getInstance() != null) IJ.beep();
 }
Example #2
0
 private boolean ignoreArrowKeys(ImagePlus imp) {
   Frame frame = WindowManager.getFrontWindow();
   String title = frame.getTitle();
   if (title != null && title.equals("ROI Manager")) return true;
   // Control Panel?
   if (frame != null && frame instanceof javax.swing.JFrame) return true;
   ImageWindow win = imp.getWindow();
   // LOCI Data Browser window?
   if (imp.getStackSize() > 1 && win != null && win.getClass().getName().startsWith("loci"))
     return true;
   return false;
 }
  public void run(String arg) {
    GenericDialog gd = new GenericDialog("Options");
    gd.addCheckbox("Acceptor_First", true);
    gd.showDialog();
    if (gd.wasCanceled()) return;
    boolean a1 = gd.getNextBoolean();
    int asp = 0;
    int dsp = 1;
    int aop = 2;
    int dop = 3;
    int atp = 4;
    int dtp = 5;
    if (!a1) {
      asp = 1;
      dsp = 0;
      aop = 3;
      dop = 2;
      atp = 5;
      dtp = 4;
    }
    ImageWindow iw = WindowManager.getCurrentWindow();
    float[][] xvals = (float[][]) jutils.runPW4VoidMethod(iw, "getXValues");
    float[][] yvals = (float[][]) jutils.runPW4VoidMethod(iw, "getYValues");
    int[] npts = (int[]) jutils.runPW4VoidMethod(iw, "getNpts");
    int maxpts = (int) jstatistics.getstatistic("Max", npts, null);
    int nsets = npts.length / 6;
    float[][] rtxvals = new float[nsets][maxpts];
    float[][] rtavals = new float[nsets][maxpts];
    float[][] rtdvals = new float[nsets][maxpts];
    int[] rtnpts = new int[nsets];
    int fretlength = 10;
    float[][] stfretvals = new float[nsets][fretlength];
    float[][] offfretvals = new float[nsets][fretlength];
    TextWindow tw = jutils.selectTable("DNA Damage FRET");
    if (tw == null)
      tw =
          new TextWindow(
              "DNA Damage FRET",
              "title\tacceptor\tdonor\testripe\teoff\tmaxart\tmaxdrt",
              "",
              400,
              200);
    // note that st stands for stripe and off is for areas off the damage stripe
    for (int i = 0; i < npts.length / 6; i++) {
      int len = npts[i * 6];
      int damageindex = findbleach(yvals[i * 6 + dsp], len);
      int fretindex = findbleach(yvals[i * 6 + atp], len);
      IJ.log("set " + i + " damage pos = " + damageindex + " , fret pos = " + fretindex);
      int predamagestart = damageindex - 4;
      int prefretstart = fretindex - 5;
      float staccpredam = getavg(yvals[i * 6 + asp], len, predamagestart, damageindex - 1);
      float stdonpredam = getavg(yvals[i * 6 + dsp], len, predamagestart, damageindex - 1);
      float nucaccpredam = getavg(yvals[i * 6 + atp], len, predamagestart, damageindex - 1);
      float nucdonpredam = getavg(yvals[i * 6 + dtp], len, predamagestart, damageindex - 1);
      float stdonprefret = getavg(yvals[i * 6 + dsp], len, prefretstart, fretindex - 2);
      float stdonafret = getavg(yvals[i * 6 + dsp], len, fretindex, fretindex + 3);
      float offdonprefret = getavg(yvals[i * 6 + dop], len, prefretstart, fretindex - 2);
      float offdonafret = getavg(yvals[i * 6 + dop], len, fretindex, fretindex + 3);
      float estripe = 1.0f - stdonprefret / stdonafret;
      float eoff = 1.0f - offdonprefret / offdonafret;
      rtnpts[i] = len;
      for (int j = 0; j < len; j++) {
        rtxvals[i][j] = j - damageindex - 1;
        rtavals[i][j] =
            (yvals[i * 6 + asp][j] / staccpredam) / (yvals[i * 6 + atp][j] / nucaccpredam);
        rtdvals[i][j] =
            (yvals[i * 6 + dsp][j] / stdonpredam) / (yvals[i * 6 + dtp][j] / nucdonpredam);
      }
      float[] smart = (float[]) algutils.get_subarray(rtavals[i], 0, fretindex);
      float[] smdrt = (float[]) algutils.get_subarray(rtdvals[i], 0, fretindex);
      jsmooth.blur1D(smart, 2.0f);
      jsmooth.blur1D(smdrt, 2.0f);
      float maxart = 0.0f;
      float maxdrt = 0.0f;
      for (int j = 0; j < fretindex - 2; j++) {
        if (smart[j] > maxart) maxart = smart[j];
        if (smdrt[j] > maxdrt) maxdrt = smdrt[j];
      }

      stfretvals[i] = getregion(yvals[i * 6 + dsp], len, prefretstart, fretlength);
      offfretvals[i] = getregion(yvals[i * 6 + dop], len, prefretstart, fretlength);
      tw.append(
          iw.getTitle()
              + "-"
              + (i + 1)
              + "\t"
              + staccpredam
              + "\t"
              + stdonpredam
              + "\t"
              + estripe
              + "\t"
              + eoff
              + "\t"
              + maxart
              + "\t"
              + maxdrt);
    }
    new PlotWindow4("Stripe_FRET_profiles", "time", "intensity", stfretvals, null).draw();
    new PlotWindow4("OffStripe_FRET_profiles", "time", "intensity", offfretvals, null).draw();
    new PlotWindow4("Acc_Rt_profiles", "time", "intensity", rtxvals, rtavals, rtnpts).draw();
    new PlotWindow4("Don_Rt_profiles", "time", "intensity", rtxvals, rtdvals, rtnpts).draw();
  }
 public void run(String arg) {
   String[] labels = {
     "Masked_Chromosomes", "Unmixed_Image", "Spectral_Image(optional)", "Spectra(optional)"
   };
   ImagePlus[] imps = jutils.selectImages(true, 4, labels);
   if (imps == null) {
     return;
   }
   if (imps[0] == null) {
     return;
   }
   float[] mask = (float[]) imps[0].getStack().getPixels(2);
   findblobs3 fb = new findblobs3(imps[0].getWidth(), imps[0].getHeight());
   float[] objects = fb.dofindblobs(mask, 0.5f);
   WaitForUserDialog dg =
       new WaitForUserDialog(
           "Optional Input", "Place RoiManager Points on Chromosome Segments (if desired)");
   dg.show();
   if (!dg.escPressed()) {
     RoiManager rman = RoiManager.getInstance();
     while (rman != null && rman.getCount() > 1) {
       Roi[] rois = rman.getRoisAsArray();
       int[] ids = new int[rois.length];
       for (int i = 0; i < rois.length; i++) {
         Rectangle r = rois[i].getBounds();
         ids[i] = (int) objects[r.x + fb.width * r.y];
       }
       objects = fb.link_objects(objects, ids);
       rman.reset();
       dg =
           new WaitForUserDialog(
               "Optional Input",
               "Place More RoiManager Points on Chromosome Segments (if desired)");
       dg.show();
       if (dg.escPressed()) break;
     }
   }
   int[] areas = fb.get_areas(objects);
   int[] temprank = jsort.get_javasort_order(areas);
   int[] arearank = jsort.get_javasort_order(temprank);
   for (int i = 0; i < fb.nobjects; i++) {
     arearank[i] = fb.nobjects - arearank[i] - 1;
   }
   // if the spectra are available, get them
   float[][][] spectra = null;
   Object[] data = null;
   if (imps[1] != null && imps[2] != null && imps[3] != null) {
     ImageWindow iw = imps[3].getWindow();
     if (iw.getClass().getName().equals("jguis.PlotWindow4")) {
       float[][] yvals = (float[][]) jutils.runPW4VoidMethod(iw, "getYValues");
       data = jutils.stack2array(imps[2].getStack());
       Object[] coef = jutils.stack2array(imps[1].getStack());
       spectra = new float[fb.nobjects][2][];
       for (int i = 0; i < fb.nobjects; i++) {
         spectra[i][0] = fb.get_object_spectrum(objects, (i + 1), data, "Sum");
         spectra[i][1] = new float[yvals[0].length];
         float[] tempcoef = fb.get_object_spectrum(objects, (i + 1), coef, "Sum");
         for (int j = 0; j < yvals[0].length; j++) {
           for (int k = 0; k < 5; k++) {
             spectra[i][1][j] += tempcoef[k] * yvals[k][j];
           }
         }
       }
     }
   }
   CompositeImage imp = (CompositeImage) imps[0];
   imp.setPosition(1, 1, 1);
   LUT graylut = jutils.get_lut_for_color(Color.white);
   imp.setChannelColorModel(graylut);
   imp.setPosition(2, 1, 1);
   LUT redlut = jutils.get_lut_for_color(Color.red);
   imp.setChannelColorModel(redlut);
   imp.setPosition(1, 1, 1);
   imp.updateAndRepaintWindow();
   SkyPanel_v3 sp = new SkyPanel_v3();
   int skychan = 6;
   if (imps[1] != null) skychan = imps[1].getNChannels();
   // assume that the sky image has 6 channels and that the second is the unknown green
   // shift the unknown green to the end
   ImagePlus skyimp = null;
   if (imps[1] != null) {
     Object[] skystack = jutils.stack2array(imps[1].getStack());
     // Object[]
     // skystack2={skystack[0],skystack[2],skystack[3],skystack[4],skystack[5],skystack[1]};
     Object[] skystack2 = null;
     if (skychan == 6)
       skystack2 = new Object[] {skystack[0], skystack[2], skystack[3], skystack[4], skystack[5]};
     else
       skystack2 = new Object[] {skystack[0], skystack[1], skystack[2], skystack[3], skystack[4]};
     skyimp =
         new ImagePlus(
             "rearranged", jutils.array2stack(skystack2, imps[1].getWidth(), imps[1].getHeight()));
   }
   int nch = 5;
   if (skyimp != null) nch = skyimp.getStack().getSize();
   GenericDialog gd2 = new GenericDialog("Options");
   gd2.addNumericField("Area Accuracy (percent)", 30, 0);
   for (int i = 0; i < nch; i++) {
     gd2.addNumericField("Ch_" + (i + 1) + "_Contr_Thresh", 0.35, 5, 15, null);
   }
   // gd2.addNumericField("Contribution Threshold",0.35,5,15,null);
   gd2.addCheckbox("Mouse?", false);
   gd2.addNumericField("Box_Width", 150, 0);
   gd2.addNumericField("Box_Height", 100, 0);
   gd2.showDialog();
   if (gd2.wasCanceled()) {
     return;
   }
   sp.areathresh = (float) gd2.getNextNumber();
   sp.objthresh2 = new float[nch];
   for (int i = 0; i < nch; i++) sp.objthresh2[i] = (float) gd2.getNextNumber();
   // sp.objthresh=(float)gd2.getNextNumber();
   boolean mouse = gd2.getNextBoolean();
   int bwidth = (int) gd2.getNextNumber();
   int bheight = (int) gd2.getNextNumber();
   int[] colorindices = {4, 1, 2, 6, 3};
   GenericDialog gd3 = new GenericDialog("Color Options");
   for (int i = 0; i < 5; i++)
     gd3.addChoice(
         "Ch" + (i + 1) + " Color",
         SkyPanel_v3.colornames,
         SkyPanel_v3.colornames[colorindices[i]]);
   gd3.showDialog();
   if (gd3.wasCanceled()) return;
   for (int i = 0; i < 5; i++) colorindices[i] = gd3.getNextChoiceIndex();
   sp.colorindices = colorindices;
   sp.nch = 5;
   sp.dapilast = false;
   sp.cellwidth = bwidth;
   sp.cellheight = bheight;
   sp.init(imps[0], skyimp, objects, areas, arearank, fb, true, spectra, data, mouse);
   SkyPanel_v3.launch_frame(sp);
 }