public void run(String arg) { ImagePlus imp = WindowManager.getCurrentImage(); if (imp == null) { IJ.noImage(); return; } ImageStack stack1 = imp.getStack(); String fileName = imp.getTitle(); int endslice = stack1.getSize(); ImagePlus imp2 = duplicateStack(imp); imp2.show(); String duplicateName = imp2.getTitle(); // IJ.showMessage("Box",fileName); ImageStack stack2 = imp2.getStack(); stack1.deleteSlice(1); stack2.deleteSlice(endslice); String calculatorstring = ("image1='" + fileName + "' operation=Subtract image2=" + imp2.getTitle() + " create stack"); IJ.run("Image Calculator...", calculatorstring); ImagePlus imp3 = WindowManager.getCurrentImage(); imp3.setTitle(fileName + " DeltaF up"); imp2.getWindow().close(); imp.getWindow().close(); }
/** * Execute plugin functionality: stack FFT with window function, max projection over all slices * (phase, Z angle), blank out central 1/8 circle (set to min value), display min-max. */ public ResultSet exec(ImagePlus... imps) { ImagePlus imp = imps[0]; Util_StackFFT2D stackFFT2D = new Util_StackFFT2D(); stackFFT2D.resultTypeChoice = Util_StackFFT2D.resultType[1]; ImagePlus impF = stackFFT2D.exec(imp); IJ.run(impF, "Z Project...", "projection=[Max Intensity]"); ImagePlus impProjF = ij.WindowManager.getCurrentImage(); maskCentralRegion(impProjF); if (impProjF.isComposite()) { // display grayscale, not colored composite CompositeImage ci = (CompositeImage) impProjF; ci.setMode(IJ.GRAYSCALE); impProjF.updateAndDraw(); } displayMinToMax(impProjF); impProjF.setTitle(I1l.makeTitle(imps[0], TLA)); String shortInfo = "Maximum intensity projection of log" + " (amplitude^2) 2D FFT stack, central region masked," + " rescaled (min-max) to improve contrast of the relevant" + " frequency range."; results.addImp(shortInfo, impProjF); results.addInfo( "How to interpret", "look for clean 1st & 2nd" + " order spots, similar across angles. Note that spot" + " intensity depends on image content."); return results; }