public synchronized void adjustmentValueChanged(AdjustmentEvent e) {

      if (!checkImage()) {
        IJ.beep();
        IJ.showStatus("No Image");
        return;
      }

      if (e.getSource() == minSlider) {
        adjustMinHue((int) minSlider.getValue());
      } else if (e.getSource() == maxSlider) {
        adjustMaxHue((int) maxSlider.getValue());
      } else if (e.getSource() == minSlider2) {
        adjustMinSat((int) minSlider2.getValue());
      } else if (e.getSource() == maxSlider2) {
        adjustMaxSat((int) maxSlider2.getValue());
      } else if (e.getSource() == minSlider3) {
        adjustMinBri((int) minSlider3.getValue());
      } else if (e.getSource() == maxSlider3) {
        adjustMaxBri((int) maxSlider3.getValue());
      }
      originalB.setEnabled(true);
      updateLabels();
      updatePlot();
      notify();
    }
Example #2
0
  public Channels() {
    super("Channels");
    if (instance != null) {
      instance.toFront();
      return;
    }
    WindowManager.addWindow(this);
    instance = this;
    GridBagLayout gridbag = new GridBagLayout();
    GridBagConstraints c = new GridBagConstraints();
    setLayout(gridbag);
    int y = 0;
    c.gridx = 0;
    c.gridy = y++;
    c.gridwidth = 1;
    c.fill = GridBagConstraints.BOTH;
    c.anchor = GridBagConstraints.CENTER;
    int margin = 32;
    if (IJ.isMacOSX()) margin = 20;
    c.insets = new Insets(10, margin, 10, margin);
    choice = new Choice();
    for (int i = 0; i < modes.length; i++) choice.addItem(modes[i]);
    choice.select(0);
    choice.addItemListener(this);
    add(choice, c);

    CompositeImage ci = getImage();
    int nCheckBoxes = ci != null ? ci.getNChannels() : 3;
    if (nCheckBoxes > CompositeImage.MAX_CHANNELS) nCheckBoxes = CompositeImage.MAX_CHANNELS;
    checkbox = new Checkbox[nCheckBoxes];
    for (int i = 0; i < nCheckBoxes; i++) {
      checkbox[i] = new Checkbox("Channel " + (i + 1), true);
      c.insets = new Insets(0, 25, i < nCheckBoxes - 1 ? 0 : 10, 5);
      c.gridy = y++;
      add(checkbox[i], c);
      checkbox[i].addItemListener(this);
    }

    c.insets = new Insets(0, 15, 10, 15);
    c.fill = GridBagConstraints.NONE;
    c.gridy = y++;
    moreButton = new Button(moreLabel);
    moreButton.addActionListener(this);
    add(moreButton, c);
    update();

    pm = new PopupMenu();
    for (int i = 0; i < menuItems.length; i++) addPopupItem(menuItems[i]);
    add(pm);

    addKeyListener(IJ.getInstance()); // ImageJ handles keyboard shortcuts
    setResizable(false);
    pack();
    if (location == null) {
      GUI.center(this);
      location = getLocation();
    } else setLocation(location);
    show();
  }
Example #3
0
 public void actionPerformed(ActionEvent e) {
   String command = e.getActionCommand();
   if (command == null) return;
   if (command.equals(moreLabel)) {
     Point bloc = moreButton.getLocation();
     pm.show(this, bloc.x, bloc.y);
   } else if (command.equals("Convert to RGB")) IJ.doCommand("Stack to RGB");
   else IJ.doCommand(command);
 }
    // GL
    public synchronized void itemStateChanged(ItemEvent e) {
      if (e.getSource() == hsb) isRGB = false;
      if (e.getSource() == rgb) isRGB = true;

      if (e.getSource() == hsb || e.getSource() == rgb) {
        flag = true;
        originalB.setEnabled(false);
        filteredB.setEnabled(false);

        minHue = minSat = minBri = 0;
        maxHue = maxSat = maxBri = 255;

        bandPassH.setState(true);
        bandPassS.setState(true);
        bandPassB.setState(true);
      }
      reset(imp, ip);
      ip = setup(imp);
      apply(imp, ip);
      updateNames();
      notify();
    }
    public void actionPerformed(ActionEvent e) {
      Button b = (Button) e.getSource();
      if (b == null) return;

      boolean imageThere = checkImage();

      if (imageThere) {
        if (b == originalB) {
          reset(imp, ip);
          filteredB.setEnabled(true);
        } else if (b == filteredB) {
          apply(imp, ip);
        } else if (b == sampleB) {
          reset(imp, ip);
          sample();
          apply(imp, ip);
        } else if (b == stackB) {
          applyStack();
        } else if (b == helpB) {
          IJ.showMessage(
              "Help",
              "Threshold Colour  v1.0\n \n"
                  + "Modification of Bob Dougherty's BandPass2 plugin by G.Landini to\n"
                  + "threshold 24 bit RGB images based on Hue, Saturation and Brightness\n"
                  + "or Red, Green and Blue components.\n \n"
                  + "Pass: Band-pass filter (anything within range is displayed).\n \n"
                  + "Stop: Band-reject filter (anything within range is NOT displayed).\n \n"
                  + "Original: Shows the original image and updates the buffer when\n"
                  + " switching to another image.\n \n"
                  + "Filtered: Shows the filtered image.\n \n"
                  + "Stack: Processes the rest of the slices in the stack (if any)\n"
                  + " using the current settings.\n \n"
                  + "Threshold: Shows the object/background in the foreground and\n"
                  + " background colours selected in the ImageJ toolbar.\n \n"
                  + "Invert: Swaps the fore/background colours.\n \n"
                  + "Sample: (experimental) Sets the ranges of the filters based on the\n"
                  + " pixel value componentd in a rectangular, user-defined, ROI.\n \n"
                  + "HSB RGB: Selects HSB or RGB space and resets all the filters.\n \n"
                  + "Note that the \'thresholded\' image is RGB, not 8 bit grey.");
        }
        updatePlot();
        updateLabels();
        imp.updateAndDraw();
      } else {
        IJ.beep();
        IJ.showStatus("No Image");
      }
      notify();
    }
Example #6
0
  void init(String[] names1, float[][][] pch1, int psfflag1) {
    setLayout(null);
    names = names1;
    pch = pch1;
    psfflag = psfflag1;
    ncurves = pch.length;
    nparams = 11;
    xpts = pch[0].length;
    ypts = pch[0][0].length;

    checkarray = new Checkbox[ncurves];
    include = new boolean[ncurves];
    namearray = new TextField[ncurves + 1];
    int1array = new TextField[ncurves + 1];
    intensity1 = new double[ncurves + 1];
    e1array = new TextField[ncurves + 1];
    n1array = new TextField[ncurves + 1];
    bright1 = new double[ncurves + 1];
    number1 = new double[ncurves + 1];
    int2array = new TextField[ncurves + 1];
    intensity2 = new double[ncurves + 1];
    e2array = new TextField[ncurves + 1];
    n2array = new TextField[ncurves + 1];
    bright2 = new double[ncurves + 1];
    number2 = new double[ncurves + 1];
    eccarray = new TextField[ncurves + 1];
    brightcc = new double[ncurves + 1];
    eminccarray = new TextField[ncurves + 1];
    brightmincc = new double[ncurves + 1];
    c2array = new TextField[ncurves + 1];
    c2 = new double[ncurves + 1];
    nmeas = new int[ncurves + 1];
    avg = new float[xpts][ypts];
    indices = new int[ncurves];
    beta = 0.05;

    getintbright();
    for (int i = 0; i < ncurves; i++) {
      include[i] = true;
      indices[i] = i;
    }
    updateavg();

    int starty = 60;
    int startx = 10;
    int yinc = 25;
    for (int i = 0; i <= ncurves; i++) {
      if (i != ncurves) {
        checkarray[i] = new Checkbox("", include[i]);
        checkarray[i].setBounds(startx, starty + i * yinc, 20, 20);
        checkarray[i].addItemListener(this);
        add(checkarray[i]);
      }

      namearray[i] = new TextField(names[i]);
      namearray[i].setBounds(startx + 30, starty + i * yinc, 200, 20);
      add(namearray[i]);

      int1array[i] = new TextField("" + (float) intensity1[i]);
      int1array[i].setBounds(startx + 30 + 210, starty + i * yinc, 40, 20);
      add(int1array[i]);

      e1array[i] = new TextField("" + (float) bright1[i]);
      e1array[i].setBounds(startx + 30 + 210 + 50, starty + i * yinc, 40, 20);
      add(e1array[i]);

      n1array[i] = new TextField("" + (float) number1[i]);
      n1array[i].setBounds(startx + 30 + 210 + 50 + 50, starty + i * yinc, 40, 20);
      add(n1array[i]);

      int2array[i] = new TextField("" + (float) intensity2[i]);
      int2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(int2array[i]);

      e2array[i] = new TextField("" + (float) bright2[i]);
      e2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(e2array[i]);

      n2array[i] = new TextField("" + (float) number2[i]);
      n2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(n2array[i]);

      eccarray[i] = new TextField("" + (float) brightcc[i]);
      eccarray[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(eccarray[i]);

      eminccarray[i] = new TextField("" + (float) brightmincc[i]);
      eminccarray[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(eminccarray[i]);

      c2[i] = 0.0;
      c2array[i] = new TextField("" + (float) c2[i]);
      c2array[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 80, 20);
      add(c2array[i]);
    }

    namelabel = new Label("Filename");
    namelabel.setBounds(startx + 30, starty - 25, 100, 20);
    add(namelabel);

    intlabel = new Label("<Ig>");
    intlabel.setBounds(startx + 30 + 210, starty - 25, 40, 20);
    add(intlabel);

    brightlabel = new Label("<eg>");
    brightlabel.setBounds(startx + 30 + 210 + 50, starty - 25, 40, 20);
    add(brightlabel);

    nlabel = new Label("<Ng>");
    nlabel.setBounds(startx + 30 + 210 + 50 + 50, starty - 25, 40, 20);
    add(nlabel);

    int2label = new Label("<Ir>");
    int2label.setBounds(startx + 30 + 210 + 50 + 50 + 50, starty - 25, 40, 20);
    add(int2label);

    bright2label = new Label("<er>");
    bright2label.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(bright2label);

    n2label = new Label("<Nr>");
    n2label.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(n2label);

    brightcclabel = new Label("<ecc>");
    brightcclabel.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(brightcclabel);

    brightccminlabel = new Label("min");
    brightccminlabel.setBounds(
        startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(brightccminlabel);

    c2label = new Label("chi^2");
    c2label.setBounds(
        startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 80, 20);
    add(c2label);

    int buttonsx = startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 90;

    fitavgbutton = new Button("Fit Avg");
    fitavgbutton.setBounds(buttonsx, starty - 25, 100, 40);
    fitavgbutton.addActionListener(this);
    add(fitavgbutton);

    fitglobalbutton = new Button("Fit Global");
    fitglobalbutton.setBounds(buttonsx, starty - 25 + 50, 100, 40);
    fitglobalbutton.addActionListener(this);
    add(fitglobalbutton);

    clearparamsbutton = new Button("Reset Fit Params");
    clearparamsbutton.setBounds(buttonsx, starty - 25 + 50 + 50, 100, 40);
    clearparamsbutton.addActionListener(this);
    add(clearparamsbutton);

    checkc2 = false;

    fitclass = new NLLSfit(this, 0.0001, 50, 0.1);
    globalfitclass = new NLLSglobalfit(this, 0.0001, 50, 0.1);
    pchfunc = new pch2D((int) ((double) xpts * 1.5), (int) ((double) ypts * 1.5), psfflag);
    avgfit = new float[xpts][ypts];
    fit = new float[ncurves][xpts][ypts];

    xvals = new float[ncurves][xpts][ypts];
    for (int i = 0; i < ncurves; i++) {
      for (int j = 0; j < xpts; j++) {
        for (int k = 0; k < ypts; k++) {
          xvals[i][j][k] = (float) k;
          fit[i][j][k] = 1.0f;
        }
      }
    }

    globalc2label = new Label("Global chi^2 = " + (float) 0.0);
    globalc2label.setBounds(buttonsx, starty - 25 + 50 + 50 + 50, 140, 20);
    add(globalc2label);

    dispcurvelabel = new Label("Display Fit #");
    dispcurvelabel.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30, 70, 20);
    add(dispcurvelabel);

    dispcurvechoice = new Choice();
    for (int i = 0; i < ncurves; i++) {
      dispcurvechoice.add("" + (i + 1));
    }
    dispcurve = 0;
    dispcurvechoice.select(0);
    dispcurvechoice.setBounds(buttonsx + 80, starty - 25 + 50 + 50 + 50 + 30, 40, 20);
    dispcurvechoice.addItemListener(this);
    add(dispcurvechoice);

    betalabel = new Label("Bleedthrough f");
    betalabel.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30, 100, 20);
    add(betalabel);
    betaval = new TextField("" + (float) beta);
    betaval.setBounds(buttonsx + 110, starty - 25 + 50 + 50 + 50 + 30 + 30, 40, 20);
    betaval.addActionListener(this);
    add(betaval);

    beta = Double.parseDouble(betaval.getText());
    updatebeta();

    undobutton = new Button("Undo Global Fit");
    undobutton.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30 + 50, 100, 40);
    undobutton.addActionListener(this);
    add(undobutton);

    geterrorsbutton = new Button("Get Errors");
    geterrorsbutton.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30 + 50 + 50, 100, 40);
    geterrorsbutton.addActionListener(this);
    add(geterrorsbutton);

    copylabel = new Label("copyright 2009 Jay Unruh ([email protected]) non-profit use only");
    copylabel.setBounds(10, 790, 400, 20);
    add(copylabel);

    n_b_label = new Label("N and B Analysis");
    n_b_label.setBounds(250, 10, 100, 20);
    add(n_b_label);

    pwavg = new PlotWindow3D("Avg", "kg", "kr", "Frequency", avg, 0);
    pwavg.setLogAxes(false, false, true);
    pwavg.draw();
    pwavg.addPoints(avgfit, true, 0);
    float[] temp = pwavg.getLimits();
    temp[4] = 1.0f;
    pwavg.setLimits(temp);

    float[][] temppch = new float[xpts][ypts];
    for (int i = 0; i < xpts; i++) {
      System.arraycopy(pch[dispcurve][i], 0, temppch[i], 0, ypts);
    }
    pwfit = new PlotWindow3D("Selected Curve", "kg", "kr", "Frequency", temppch, 0);
    pwfit.setLogAxes(false, false, true);
    pwfit.draw();
    pwfit.addPoints(fit[dispcurve], true, 0);
    float[] temp2 = pwfit.getLimits();
    temp2[4] = 1.0f;
    pwfit.setLimits(temp2);

    resetparams();
    repaint();
  }
    public BandAdjuster() {

      super("Threshold Colour");
      if (instance != null) {
        instance.toFront();
        return;
      }
      imp = WindowManager.getCurrentImage();
      if (imp == null) {
        IJ.beep();
        IJ.showStatus("No image");
        return;
      }
      IJ.run("Select None");
      thread = new Thread(this, "BandAdjuster");
      WindowManager.addWindow(this);
      instance = this;
      IJ.register(PasteController.class);

      ij = IJ.getInstance();
      Font font = new Font("SansSerif", Font.PLAIN, 10);
      GridBagLayout gridbag = new GridBagLayout();
      GridBagConstraints c = new GridBagConstraints();
      setLayout(gridbag);

      int y = 0;
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      labelh = new Label("Hue", Label.CENTER);
      add(labelh, c);

      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(7, 0, 0, 0);
      labelf = new Label("Filter type", Label.RIGHT);
      add(labelf, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(plot, c);

      // checkboxes
      panelh = new Panel();
      filterTypeH = new CheckboxGroup();
      bandPassH = new Checkbox("Pass");
      bandPassH.setCheckboxGroup(filterTypeH);
      bandPassH.addItemListener(this);
      panelh.add(bandPassH);
      bandStopH = new Checkbox("Stop");
      bandStopH.setCheckboxGroup(filterTypeH);
      bandStopH.addItemListener(this);
      panelh.add(bandStopH);
      bandPassH.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelh, c);

      // minHue slider
      minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);

      add(minSlider, c);
      minSlider.addAdjustmentListener(this);
      minSlider.setUnitIncrement(1);

      // minHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label1 = new Label("       ", Label.LEFT);
      label1.setFont(font);
      add(label1, c);

      // maxHue sliderHue
      maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider, c);
      maxSlider.addAdjustmentListener(this);
      maxSlider.setUnitIncrement(1);

      // maxHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label2 = new Label("       ", Label.LEFT);
      label2.setFont(font);
      add(label2, c);

      // =====
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labels = new Label("Saturation", Label.CENTER);
      add(labels, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(splot, c);

      // checkboxes
      panels = new Panel();
      filterTypeS = new CheckboxGroup();
      bandPassS = new Checkbox("Pass");
      bandPassS.setCheckboxGroup(filterTypeS);
      bandPassS.addItemListener(this);
      panels.add(bandPassS);
      bandStopS = new Checkbox("Stop");
      bandStopS.setCheckboxGroup(filterTypeS);
      bandStopS.addItemListener(this);
      panels.add(bandStopS);
      bandPassS.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panels, c);

      // minSat slider
      minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider2, c);
      minSlider2.addAdjustmentListener(this);
      minSlider2.setUnitIncrement(1);

      // minSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label3 = new Label("       ", Label.LEFT);
      label3.setFont(font);
      add(label3, c);

      // maxSat slider
      maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider2, c);
      maxSlider2.addAdjustmentListener(this);
      maxSlider2.setUnitIncrement(1);

      // maxSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label4 = new Label("       ", Label.LEFT);
      label4.setFont(font);
      add(label4, c);

      // =====
      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labelb = new Label("Brightness", Label.CENTER);
      add(labelb, c);

      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(bplot, c);

      // checkboxes
      panelb = new Panel();
      filterTypeB = new CheckboxGroup();
      bandPassB = new Checkbox("Pass");
      bandPassB.setCheckboxGroup(filterTypeB);
      bandPassB.addItemListener(this);
      panelb.add(bandPassB);
      bandStopB = new Checkbox("Stop");
      bandStopB.setCheckboxGroup(filterTypeB);
      bandStopB.addItemListener(this);
      panelb.add(bandStopB);
      bandPassB.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelb, c);

      // minBri slider
      minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider3, c);
      minSlider3.addAdjustmentListener(this);
      minSlider3.setUnitIncrement(1);

      // minBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label5 = new Label("       ", Label.LEFT);
      label5.setFont(font);
      add(label5, c);

      // maxBri slider
      maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider3, c);
      maxSlider3.addAdjustmentListener(this);
      maxSlider3.setUnitIncrement(1);

      // maxBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label6 = new Label("       ", Label.LEFT);
      label6.setFont(font);
      add(label6, c);

      // =====
      panelt = new Panel();
      threshold = new Checkbox("Threshold");
      threshold.addItemListener(this);
      panelt.add(threshold);

      invert = new Checkbox("Invert");
      invert.addItemListener(this);
      panelt.add(invert);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelt, c);

      // buttons
      panel = new Panel();
      // panel.setLayout(new GridLayout(2, 2, 0, 0));
      originalB = new Button("Original");
      originalB.setEnabled(false);
      originalB.addActionListener(this);
      originalB.addKeyListener(ij);
      panel.add(originalB);

      filteredB = new Button("Filtered");
      filteredB.setEnabled(false);
      filteredB.addActionListener(this);
      filteredB.addKeyListener(ij);
      panel.add(filteredB);

      stackB = new Button("Stack");
      stackB.addActionListener(this);
      stackB.addKeyListener(ij);
      panel.add(stackB);

      helpB = new Button("Help");
      helpB.addActionListener(this);
      helpB.addKeyListener(ij);
      panel.add(helpB);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panel, c);

      panelMode = new Panel();

      sampleB = new Button("Sample");
      sampleB.addActionListener(this);
      sampleB.addKeyListener(ij);
      panelMode.add(sampleB);

      colourMode = new CheckboxGroup();
      hsb = new Checkbox("HSB");
      hsb.setCheckboxGroup(colourMode);
      hsb.addItemListener(this);
      panelMode.add(hsb);
      hsb.setState(true);
      rgb = new Checkbox("RGB");
      rgb.setCheckboxGroup(colourMode);
      rgb.addItemListener(this);
      panelMode.add(rgb);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelMode, c);

      addKeyListener(ij); // ImageJ handles keyboard shortcuts
      pack();
      GUI.center(this);
      setVisible(true);

      ip = setup(imp);
      if (ip == null) {
        imp.unlock();
        IJ.beep();
        IJ.showStatus("RGB image cannot be thresholded");
        return;
      }
      thread.start();
    }
    void sample() {
      byte[] hsSource, ssSource, bsSource;
      // ImageProcessor ip2;
      // ip2 = imp.getProcessor();
      Rectangle myroi = ip.getRoi();
      int swidth = myroi.width;
      int sheight = myroi.height;
      int sy = myroi.y;
      int sx = myroi.x;
      if (swidth == width && sheight == height) {
        IJ.showMessage("Select a rectangular ROI");
        IJ.beep();
        return;
      }

      IJ.run("Select None");

      int snumPixels = swidth * sheight;

      hsSource = new byte[snumPixels];
      ssSource = new byte[snumPixels];
      bsSource = new byte[snumPixels];

      int[] pixs = new int[snumPixels];
      int[] bin = new int[256];

      int counter = 0, pi = 0, rangePassH = 0, rangeStopH = 0, rangePassL = 0, rangeStopL = 0, i, j;

      for (i = sy; i < sy + sheight; i++) {
        for (j = sx; j < sx + swidth; j++) {
          pixs[counter++] = ip.getPixel(j, i);
        }
      }

      // Get hsb or rgb from roi.
      ColorProcessor cp2 = new ColorProcessor(swidth, sheight, pixs);

      int iminhue = 256, imaxhue = -1, iminsat = 256, imaxsat = -1, iminbri = 256, imaxbri = -1;
      int iminred = 256, imaxred = -1, imingre = 256, imaxgre = -1, iminblu = 256, imaxblu = -1;

      if (isRGB) cp2.getRGB(hsSource, ssSource, bsSource);
      else cp2.getHSB(hsSource, ssSource, bsSource);

      for (i = 0; i < snumPixels; i++) {
        bin[hsSource[i] & 255] = 1;
        if ((hsSource[i] & 255) > imaxhue) imaxhue = (hsSource[i] & 255);
        if ((hsSource[i] & 255) < iminhue) iminhue = (hsSource[i] & 255);
        if ((ssSource[i] & 255) > imaxsat) imaxsat = (ssSource[i] & 255);
        if ((ssSource[i] & 255) < iminsat) iminsat = (ssSource[i] & 255);
        if ((bsSource[i] & 255) > imaxbri) imaxbri = (bsSource[i] & 255);
        if ((bsSource[i] & 255) < iminbri) iminbri = (bsSource[i] & 255);
        // IJ.showMessage("h:"+minhue+"H:"+maxhue+"s:"+minsat+"S:"+maxsat+"b:"+minbri+"B:"+maxbri);
      }

      if (!isRGB) { // get pass or stop filter whichever has a narrower range
        for (i = 0; i < 256; i++) {
          if (bin[i] > 0) {
            rangePassL = i;
            break;
          }
        }
        for (i = 255; i >= 0; i--) {
          if (bin[i] > 0) {
            rangePassH = i;
            break;
          }
        }
        for (i = 0; i < 256; i++) {
          if (bin[i] == 0) {
            rangeStopL = i;
            break;
          }
        }
        for (i = 255; i >= 0; i--) {
          if (bin[i] == 0) {
            rangeStopH = i;
            break;
          }
        }
        if ((rangePassH - rangePassL) < (rangeStopH - rangeStopL)) {
          bandPassH.setState(true);
          bandStopH.setState(false);
          iminhue = rangePassL;
          imaxhue = rangePassH;
        } else {
          bandPassH.setState(false);
          bandStopH.setState(true);
          iminhue = rangeStopL;
          imaxhue = rangeStopH;
        }
      } else {
        bandPassH.setState(true);
        bandStopH.setState(false);
      }

      adjustMinHue(iminhue);
      minSlider.setValue(iminhue);
      adjustMaxHue(imaxhue);
      maxSlider.setValue(imaxhue);
      adjustMinSat(iminsat);
      minSlider2.setValue(iminsat);
      adjustMaxSat(imaxsat);
      maxSlider2.setValue(imaxsat);
      adjustMinBri(iminbri);
      minSlider3.setValue(iminbri);
      adjustMaxBri(imaxbri);
      maxSlider3.setValue(imaxbri);
      originalB.setEnabled(true);
      // IJ.showStatus("done");
    }