public ImageCanvas(ImagePlus imp) { this.imp = imp; ij = IJ.getInstance(); int width = imp.getWidth(); int height = imp.getHeight(); imageWidth = width; imageHeight = height; srcRect = new Rectangle(0, 0, imageWidth, imageHeight); setDrawingSize(imageWidth, (int) (imageHeight)); magnification = 1.0; addMouseListener(this); addMouseMotionListener(this); addKeyListener(ij); // ImageJ handles keyboard shortcuts setFocusTraversalKeysEnabled(false); }
/** Restores original disk or network version of image. */ public void revertToSaved(ImagePlus imp) { Image img; ProgressBar pb = IJ.getInstance().getProgressBar(); ImageProcessor ip; String path = fi.directory + fi.fileName; if (fi.fileFormat == fi.GIF_OR_JPG) { // restore gif or jpg img = Toolkit.getDefaultToolkit().createImage(path); imp.setImage(img); if (imp.getType() == ImagePlus.COLOR_RGB) Opener.convertGrayJpegTo8Bits(imp); return; } if (fi.fileFormat == fi.DICOM) { // restore DICOM ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.DICOM", path); if (imp2 != null) imp.setProcessor(null, imp2.getProcessor()); return; } if (fi.fileFormat == fi.BMP) { // restore BMP ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.BMP_Reader", path); if (imp2 != null) imp.setProcessor(null, imp2.getProcessor()); return; } if (fi.fileFormat == fi.PGM) { // restore PGM ImagePlus imp2 = (ImagePlus) IJ.runPlugIn("ij.plugin.PGM_Reader", path); if (imp2 != null) imp.setProcessor(null, imp2.getProcessor()); return; } if (fi.fileFormat == fi.ZIP_ARCHIVE) { // restore ".zip" file ImagePlus imp2 = (new Opener()).openZip(path); if (imp2 != null) imp.setProcessor(null, imp2.getProcessor()); return; } // restore PNG or another image opened using ImageIO if (fi.fileFormat == fi.IMAGEIO) { ImagePlus imp2 = (new Opener()).openUsingImageIO(path); if (imp2 != null) imp.setProcessor(null, imp2.getProcessor()); return; } if (fi.nImages > 1) return; ColorModel cm; if (fi.url == null || fi.url.equals("")) IJ.showStatus("Loading: " + path); else IJ.showStatus("Loading: " + fi.url + fi.fileName); Object pixels = readPixels(fi); if (pixels == null) return; cm = createColorModel(fi); switch (fi.fileType) { case FileInfo.GRAY8: case FileInfo.COLOR8: case FileInfo.BITMAP: ip = new ByteProcessor(width, height, (byte[]) pixels, cm); imp.setProcessor(null, ip); break; case FileInfo.GRAY16_SIGNED: case FileInfo.GRAY16_UNSIGNED: case FileInfo.GRAY12_UNSIGNED: ip = new ShortProcessor(width, height, (short[]) pixels, cm); imp.setProcessor(null, ip); break; case FileInfo.GRAY32_INT: case FileInfo.GRAY32_FLOAT: ip = new FloatProcessor(width, height, (float[]) pixels, cm); imp.setProcessor(null, ip); break; case FileInfo.RGB: case FileInfo.BGR: case FileInfo.ARGB: case FileInfo.ABGR: case FileInfo.RGB_PLANAR: img = Toolkit.getDefaultToolkit() .createImage(new MemoryImageSource(width, height, (int[]) pixels, 0, width)); imp.setImage(img); break; } }
/** * Performs particle analysis on the specified ImagePlus and ImageProcessor. Returns false if * there is an error. */ public boolean analyze(ImagePlus imp, ImageProcessor ip) { if (this.imp == null) this.imp = imp; showResults = (options & SHOW_RESULTS) != 0; excludeEdgeParticles = (options & EXCLUDE_EDGE_PARTICLES) != 0; resetCounter = (options & CLEAR_WORKSHEET) != 0; showProgress = (options & SHOW_PROGRESS) != 0; floodFill = (options & INCLUDE_HOLES) == 0; recordStarts = (options & RECORD_STARTS) != 0; addToManager = (options & ADD_TO_MANAGER) != 0; displaySummary = (options & DISPLAY_SUMMARY) != 0; inSituShow = (options & IN_SITU_SHOW) != 0; outputImage = null; ip.snapshot(); ip.setProgressBar(null); if (Analyzer.isRedirectImage()) { redirectImp = Analyzer.getRedirectImage(imp); if (redirectImp == null) return false; int depth = redirectImp.getStackSize(); if (depth > 1 && depth == imp.getStackSize()) { ImageStack redirectStack = redirectImp.getStack(); redirectIP = redirectStack.getProcessor(imp.getCurrentSlice()); } else redirectIP = redirectImp.getProcessor(); } else if (imp.getType() == ImagePlus.COLOR_RGB) { ImagePlus original = (ImagePlus) imp.getProperty("OriginalImage"); if (original != null && original.getWidth() == imp.getWidth() && original.getHeight() == imp.getHeight()) { redirectImp = original; redirectIP = original.getProcessor(); } } if (!setThresholdLevels(imp, ip)) return false; width = ip.getWidth(); height = ip.getHeight(); if (!(showChoice == NOTHING || showChoice == OVERLAY_OUTLINES || showChoice == OVERLAY_MASKS)) { blackBackground = Prefs.blackBackground && inSituShow; if (slice == 1) outlines = new ImageStack(width, height); if (showChoice == ROI_MASKS) drawIP = new ShortProcessor(width, height); else drawIP = new ByteProcessor(width, height); drawIP.setLineWidth(lineWidth); if (showChoice == ROI_MASKS) { } // Place holder for now... else if (showChoice == MASKS && !blackBackground) drawIP.invertLut(); else if (showChoice == OUTLINES) { if (!inSituShow) { if (customLut == null) makeCustomLut(); drawIP.setColorModel(customLut); } drawIP.setFont(new Font("SansSerif", Font.PLAIN, fontSize)); if (fontSize > 12 && inSituShow) drawIP.setAntialiasedText(true); } outlines.addSlice(null, drawIP); if (showChoice == ROI_MASKS || blackBackground) { drawIP.setColor(Color.black); drawIP.fill(); drawIP.setColor(Color.white); } else { drawIP.setColor(Color.white); drawIP.fill(); drawIP.setColor(Color.black); } } calibration = redirectImp != null ? redirectImp.getCalibration() : imp.getCalibration(); if (rt == null) { rt = Analyzer.getResultsTable(); analyzer = new Analyzer(imp); } else analyzer = new Analyzer(imp, measurements, rt); if (resetCounter && slice == 1) { if (!Analyzer.resetCounter()) return false; } beginningCount = Analyzer.getCounter(); byte[] pixels = null; if (ip instanceof ByteProcessor) pixels = (byte[]) ip.getPixels(); if (r == null) { r = ip.getRoi(); mask = ip.getMask(); if (displaySummary) { if (mask != null) totalArea = ImageStatistics.getStatistics(ip, AREA, calibration).area; else totalArea = r.width * calibration.pixelWidth * r.height * calibration.pixelHeight; } } minX = r.x; maxX = r.x + r.width; minY = r.y; maxY = r.y + r.height; if (r.width < width || r.height < height || mask != null) { if (!eraseOutsideRoi(ip, r, mask)) return false; } int offset; double value; int inc = Math.max(r.height / 25, 1); int mi = 0; ImageWindow win = imp.getWindow(); if (win != null) win.running = true; if (measurements == 0) measurements = Analyzer.getMeasurements(); if (showChoice == ELLIPSES) measurements |= ELLIPSE; measurements &= ~LIMIT; // ignore "Limit to Threshold" roiNeedsImage = (measurements & PERIMETER) != 0 || (measurements & SHAPE_DESCRIPTORS) != 0 || (measurements & FERET) != 0; particleCount = 0; wand = new Wand(ip); pf = new PolygonFiller(); if (floodFill) { ImageProcessor ipf = ip.duplicate(); ipf.setValue(fillColor); ff = new FloodFiller(ipf); } roiType = Wand.allPoints() ? Roi.FREEROI : Roi.TRACED_ROI; for (int y = r.y; y < (r.y + r.height); y++) { offset = y * width; for (int x = r.x; x < (r.x + r.width); x++) { if (pixels != null) value = pixels[offset + x] & 255; else if (imageType == SHORT) value = ip.getPixel(x, y); else value = ip.getPixelValue(x, y); if (value >= level1 && value <= level2) analyzeParticle(x, y, imp, ip); } if (showProgress && ((y % inc) == 0)) IJ.showProgress((double) (y - r.y) / r.height); if (win != null) canceled = !win.running; if (canceled) { Macro.abort(); break; } } if (showProgress) IJ.showProgress(1.0); if (showResults) rt.updateResults(); imp.killRoi(); ip.resetRoi(); ip.reset(); if (displaySummary && IJ.getInstance() != null) updateSliceSummary(); if (addToManager && roiManager != null) roiManager.setEditMode(imp, true); maxParticleCount = (particleCount > maxParticleCount) ? particleCount : maxParticleCount; totalCount += particleCount; if (!canceled) showResults(); return true; }
String getInfo(ImagePlus imp, ImageProcessor ip) { String s = new String(""); if (IJ.getInstance() != null) s += IJ.getInstance().getInfo() + "\n \n"; s += "Title: " + imp.getTitle() + "\n"; Calibration cal = imp.getCalibration(); int stackSize = imp.getStackSize(); int channels = imp.getNChannels(); int slices = imp.getNSlices(); int frames = imp.getNFrames(); int digits = imp.getBitDepth() == 32 ? 4 : 0; int dp, dp2; boolean nonUniformUnits = !cal.getXUnit().equals(cal.getYUnit()); String xunit = cal.getXUnit(); String yunit = cal.getYUnit(); String zunit = cal.getZUnit(); if (cal.scaled()) { String xunits = cal.getUnits(); String yunits = xunits; String zunits = xunits; if (nonUniformUnits) { xunits = xunit; yunits = yunit; zunits = zunit; } double pw = imp.getWidth() * cal.pixelWidth; double ph = imp.getHeight() * cal.pixelHeight; s += "Width: " + d2s(pw) + " " + xunits + " (" + imp.getWidth() + ")\n"; s += "Height: " + d2s(ph) + " " + yunits + " (" + imp.getHeight() + ")\n"; if (slices > 1) { double pd = slices * cal.pixelDepth; s += "Depth: " + d2s(pd) + " " + zunits + " (" + slices + ")\n"; } s += "Size: " + ImageWindow.getImageSize(imp) + "\n"; double xResolution = 1.0 / cal.pixelWidth; double yResolution = 1.0 / cal.pixelHeight; if (xResolution == yResolution) s += "Resolution: " + d2s(xResolution) + " pixels per " + xunit + "\n"; else { s += "X Resolution: " + d2s(xResolution) + " pixels per " + xunit + "\n"; s += "Y Resolution: " + d2s(yResolution) + " pixels per " + yunit + "\n"; } } else { s += "Width: " + imp.getWidth() + " pixels\n"; s += "Height: " + imp.getHeight() + " pixels\n"; if (stackSize > 1) s += "Depth: " + slices + " pixels\n"; s += "Size: " + ImageWindow.getImageSize(imp) + "\n"; } if (stackSize > 1) { String vunit = cal.getUnit() + "^3"; if (nonUniformUnits) vunit = "(" + xunit + " x " + yunit + " x " + zunit + ")"; s += "Voxel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + "x" + d2s(cal.pixelDepth) + " " + vunit + "\n"; } else { String punit = cal.getUnit() + "^2"; if (nonUniformUnits) punit = "(" + xunit + " x " + yunit + ")"; dp = Tools.getDecimalPlaces(cal.pixelWidth, cal.pixelHeight); s += "Pixel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + " " + punit + "\n"; } s += "ID: " + imp.getID() + "\n"; int type = imp.getType(); switch (type) { case ImagePlus.GRAY8: s += "Bits per pixel: 8 "; String lut = "LUT"; if (imp.getProcessor().isColorLut()) lut = "color " + lut; else lut = "grayscale " + lut; if (imp.isInvertedLut()) lut = "inverting " + lut; s += "(" + lut + ")\n"; if (imp.getNChannels() > 1) s += displayRanges(imp); else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n"; break; case ImagePlus.GRAY16: case ImagePlus.GRAY32: if (type == ImagePlus.GRAY16) { String sign = cal.isSigned16Bit() ? "signed" : "unsigned"; s += "Bits per pixel: 16 (" + sign + ")\n"; } else s += "Bits per pixel: 32 (float)\n"; if (imp.getNChannels() > 1) s += displayRanges(imp); else { s += "Display range: "; double min = ip.getMin(); double max = ip.getMax(); if (cal.calibrated()) { min = cal.getCValue((int) min); max = cal.getCValue((int) max); } s += d2s(min) + " - " + d2s(max) + "\n"; } break; case ImagePlus.COLOR_256: s += "Bits per pixel: 8 (color LUT)\n"; break; case ImagePlus.COLOR_RGB: s += "Bits per pixel: 32 (RGB)\n"; break; } double interval = cal.frameInterval; double fps = cal.fps; if (stackSize > 1) { ImageStack stack = imp.getStack(); int slice = imp.getCurrentSlice(); String number = slice + "/" + stackSize; String label = stack.getShortSliceLabel(slice); if (label != null && label.length() > 0) label = " (" + label + ")"; else label = ""; if (interval > 0.0 || fps != 0.0) { s += "Frame: " + number + label + "\n"; if (fps != 0.0) { String sRate = Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5); s += "Frame rate: " + sRate + " fps\n"; } if (interval != 0.0) s += "Frame interval: " + ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5)) + " " + cal.getTimeUnit() + "\n"; } else s += "Image: " + number + label + "\n"; if (imp.isHyperStack()) { if (channels > 1) s += " Channel: " + imp.getChannel() + "/" + channels + "\n"; if (slices > 1) s += " Slice: " + imp.getSlice() + "/" + slices + "\n"; if (frames > 1) s += " Frame: " + imp.getFrame() + "/" + frames + "\n"; } if (imp.isComposite()) { if (!imp.isHyperStack() && channels > 1) s += " Channels: " + channels + "\n"; String mode = ((CompositeImage) imp).getModeAsString(); s += " Composite mode: \"" + mode + "\"\n"; } } if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No threshold\n"; else { double lower = ip.getMinThreshold(); double upper = ip.getMaxThreshold(); if (cal.calibrated()) { lower = cal.getCValue((int) lower); upper = cal.getCValue((int) upper); } s += "Threshold: " + d2s(lower) + "-" + d2s(upper) + "\n"; } ImageCanvas ic = imp.getCanvas(); double mag = ic != null ? ic.getMagnification() : 1.0; if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n"; if (cal.calibrated()) { s += " \n"; int curveFit = cal.getFunction(); s += "Calibration function: "; if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n"; else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n"; else s += CurveFitter.fList[curveFit] + "\n"; double[] c = cal.getCoefficients(); if (c != null) { s += " a: " + IJ.d2s(c[0], 6) + "\n"; s += " b: " + IJ.d2s(c[1], 6) + "\n"; if (c.length >= 3) s += " c: " + IJ.d2s(c[2], 6) + "\n"; if (c.length >= 4) s += " c: " + IJ.d2s(c[3], 6) + "\n"; if (c.length >= 5) s += " c: " + IJ.d2s(c[4], 6) + "\n"; } s += " Unit: \"" + cal.getValueUnit() + "\"\n"; } else s += "Uncalibrated\n"; FileInfo fi = imp.getOriginalFileInfo(); if (fi != null) { if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n"; else if (fi.directory != null && fi.fileName != null) s += "Path: " + fi.directory + fi.fileName + "\n"; } ImageWindow win = imp.getWindow(); if (win != null) { Point loc = win.getLocation(); Dimension screen = IJ.getScreenSize(); s += "Screen location: " + loc.x + "," + loc.y + " (" + screen.width + "x" + screen.height + ")\n"; } String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : ""; String origin = d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin; if (!origin.equals("0,0") || cal.getInvertY()) s += "Coordinate origin: " + origin + "\n"; if (cal.getInvertY()) s += "Inverted y coordinates\n"; Overlay overlay = imp.getOverlay(); if (overlay != null) { String hidden = imp.getHideOverlay() ? " (hidden)" : " "; int n = overlay.size(); String elements = n == 1 ? " element" : " elements"; s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n"; } else s += "No overlay\n"; Roi roi = imp.getRoi(); if (roi == null) { if (cal.calibrated()) s += " \n"; s += "No selection\n"; } else if (roi instanceof EllipseRoi) { s += "\nElliptical selection\n"; double[] p = ((EllipseRoi) roi).getParams(); double dx = p[2] - p[0]; double dy = p[3] - p[1]; double major = Math.sqrt(dx * dx + dy * dy); s += " Major: " + IJ.d2s(major, 2) + "\n"; s += " Minor: " + IJ.d2s(major * p[4], 2) + "\n"; s += " X1: " + IJ.d2s(p[0], 2) + "\n"; s += " Y1: " + IJ.d2s(p[1], 2) + "\n"; s += " X2: " + IJ.d2s(p[2], 2) + "\n"; s += " Y2: " + IJ.d2s(p[3], 2) + "\n"; s += " Aspect ratio: " + IJ.d2s(p[4], 2) + "\n"; } else { s += " \n"; s += roi.getTypeAsString() + " Selection"; String points = null; if (roi instanceof PointRoi) { int npoints = ((PolygonRoi) roi).getNCoordinates(); String suffix = npoints > 1 ? "s)" : ")"; points = " (" + npoints + " point" + suffix; } String name = roi.getName(); if (name != null) { s += " (\"" + name + "\")"; if (points != null) s += "\n " + points; } else if (points != null) s += points; s += "\n"; Rectangle r = roi.getBounds(); if (roi instanceof Line) { Line line = (Line) roi; s += " X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n"; s += " Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n"; s += " X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n"; s += " Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n"; } else if (cal.scaled()) { s += " X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n"; s += " Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n"; s += " Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n"; s += " Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n"; } else { s += " X: " + r.x + "\n"; s += " Y: " + yy(r.y, imp) + "\n"; s += " Width: " + r.width + "\n"; s += " Height: " + r.height + "\n"; } } return s; }
public BandAdjuster() { super("Threshold Colour"); if (instance != null) { instance.toFront(); return; } imp = WindowManager.getCurrentImage(); if (imp == null) { IJ.beep(); IJ.showStatus("No image"); return; } IJ.run("Select None"); thread = new Thread(this, "BandAdjuster"); WindowManager.addWindow(this); instance = this; IJ.register(PasteController.class); ij = IJ.getInstance(); Font font = new Font("SansSerif", Font.PLAIN, 10); GridBagLayout gridbag = new GridBagLayout(); GridBagConstraints c = new GridBagConstraints(); setLayout(gridbag); int y = 0; c.gridx = 0; c.gridy = y; c.gridwidth = 1; c.weightx = 0; c.insets = new Insets(5, 0, 0, 0); labelh = new Label("Hue", Label.CENTER); add(labelh, c); c.gridx = 1; c.gridy = y++; c.gridwidth = 1; c.weightx = 0; c.insets = new Insets(7, 0, 0, 0); labelf = new Label("Filter type", Label.RIGHT); add(labelf, c); // plot c.gridx = 0; c.gridy = y; c.gridwidth = 1; c.fill = c.BOTH; c.anchor = c.CENTER; c.insets = new Insets(0, 5, 0, 0); add(plot, c); // checkboxes panelh = new Panel(); filterTypeH = new CheckboxGroup(); bandPassH = new Checkbox("Pass"); bandPassH.setCheckboxGroup(filterTypeH); bandPassH.addItemListener(this); panelh.add(bandPassH); bandStopH = new Checkbox("Stop"); bandStopH.setCheckboxGroup(filterTypeH); bandStopH.addItemListener(this); panelh.add(bandStopH); bandPassH.setState(true); c.gridx = 1; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(5, 0, 0, 0); add(panelh, c); // minHue slider minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 90 : 100; c.fill = c.HORIZONTAL; c.insets = new Insets(5, 5, 0, 0); add(minSlider, c); minSlider.addAdjustmentListener(this); minSlider.setUnitIncrement(1); // minHue slider label c.gridx = 1; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 10 : 0; c.insets = new Insets(5, 0, 0, 0); label1 = new Label(" ", Label.LEFT); label1.setFont(font); add(label1, c); // maxHue sliderHue maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y; c.gridwidth = 1; c.weightx = 100; c.insets = new Insets(5, 5, 0, 0); add(maxSlider, c); maxSlider.addAdjustmentListener(this); maxSlider.setUnitIncrement(1); // maxHue slider label c.gridx = 1; c.gridwidth = 1; c.gridy = y++; c.weightx = 0; c.insets = new Insets(5, 0, 0, 0); label2 = new Label(" ", Label.LEFT); label2.setFont(font); add(label2, c); // ===== c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = 0; c.insets = new Insets(10, 0, 0, 0); labels = new Label("Saturation", Label.CENTER); add(labels, c); // plot c.gridx = 0; c.gridy = y; c.gridwidth = 1; c.fill = c.BOTH; c.anchor = c.CENTER; c.insets = new Insets(0, 5, 0, 0); add(splot, c); // checkboxes panels = new Panel(); filterTypeS = new CheckboxGroup(); bandPassS = new Checkbox("Pass"); bandPassS.setCheckboxGroup(filterTypeS); bandPassS.addItemListener(this); panels.add(bandPassS); bandStopS = new Checkbox("Stop"); bandStopS.setCheckboxGroup(filterTypeS); bandStopS.addItemListener(this); panels.add(bandStopS); bandPassS.setState(true); c.gridx = 1; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(5, 0, 0, 0); add(panels, c); // minSat slider minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 90 : 100; c.fill = c.HORIZONTAL; c.insets = new Insets(5, 5, 0, 0); add(minSlider2, c); minSlider2.addAdjustmentListener(this); minSlider2.setUnitIncrement(1); // minSat slider label c.gridx = 1; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 10 : 0; c.insets = new Insets(5, 0, 0, 0); label3 = new Label(" ", Label.LEFT); label3.setFont(font); add(label3, c); // maxSat slider maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = 100; c.insets = new Insets(5, 5, 0, 0); add(maxSlider2, c); maxSlider2.addAdjustmentListener(this); maxSlider2.setUnitIncrement(1); // maxSat slider label c.gridx = 1; c.gridwidth = 1; c.weightx = 0; c.insets = new Insets(5, 0, 0, 0); label4 = new Label(" ", Label.LEFT); label4.setFont(font); add(label4, c); // ===== c.gridx = 0; c.gridwidth = 1; c.gridy = y++; c.weightx = 0; c.insets = new Insets(10, 0, 0, 0); labelb = new Label("Brightness", Label.CENTER); add(labelb, c); c.gridx = 0; c.gridwidth = 1; c.gridy = y; c.fill = c.BOTH; c.anchor = c.CENTER; c.insets = new Insets(0, 5, 0, 0); add(bplot, c); // checkboxes panelb = new Panel(); filterTypeB = new CheckboxGroup(); bandPassB = new Checkbox("Pass"); bandPassB.setCheckboxGroup(filterTypeB); bandPassB.addItemListener(this); panelb.add(bandPassB); bandStopB = new Checkbox("Stop"); bandStopB.setCheckboxGroup(filterTypeB); bandStopB.addItemListener(this); panelb.add(bandStopB); bandPassB.setState(true); c.gridx = 1; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(5, 0, 0, 0); add(panelb, c); // minBri slider minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 90 : 100; c.fill = c.HORIZONTAL; c.insets = new Insets(5, 5, 0, 0); add(minSlider3, c); minSlider3.addAdjustmentListener(this); minSlider3.setUnitIncrement(1); // minBri slider label c.gridx = 1; c.gridwidth = 1; c.weightx = IJ.isMacintosh() ? 10 : 0; c.insets = new Insets(5, 0, 0, 0); label5 = new Label(" ", Label.LEFT); label5.setFont(font); add(label5, c); // maxBri slider maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange); c.gridx = 0; c.gridy = y++; c.gridwidth = 1; c.weightx = 100; c.insets = new Insets(5, 5, 0, 0); add(maxSlider3, c); maxSlider3.addAdjustmentListener(this); maxSlider3.setUnitIncrement(1); // maxBri slider label c.gridx = 1; c.gridwidth = 1; c.weightx = 0; c.insets = new Insets(5, 0, 0, 0); label6 = new Label(" ", Label.LEFT); label6.setFont(font); add(label6, c); // ===== panelt = new Panel(); threshold = new Checkbox("Threshold"); threshold.addItemListener(this); panelt.add(threshold); invert = new Checkbox("Invert"); invert.addItemListener(this); panelt.add(invert); c.gridx = 0; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(0, 0, 0, 0); add(panelt, c); // buttons panel = new Panel(); // panel.setLayout(new GridLayout(2, 2, 0, 0)); originalB = new Button("Original"); originalB.setEnabled(false); originalB.addActionListener(this); originalB.addKeyListener(ij); panel.add(originalB); filteredB = new Button("Filtered"); filteredB.setEnabled(false); filteredB.addActionListener(this); filteredB.addKeyListener(ij); panel.add(filteredB); stackB = new Button("Stack"); stackB.addActionListener(this); stackB.addKeyListener(ij); panel.add(stackB); helpB = new Button("Help"); helpB.addActionListener(this); helpB.addKeyListener(ij); panel.add(helpB); c.gridx = 0; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(0, 0, 0, 0); add(panel, c); panelMode = new Panel(); sampleB = new Button("Sample"); sampleB.addActionListener(this); sampleB.addKeyListener(ij); panelMode.add(sampleB); colourMode = new CheckboxGroup(); hsb = new Checkbox("HSB"); hsb.setCheckboxGroup(colourMode); hsb.addItemListener(this); panelMode.add(hsb); hsb.setState(true); rgb = new Checkbox("RGB"); rgb.setCheckboxGroup(colourMode); rgb.addItemListener(this); panelMode.add(rgb); c.gridx = 0; c.gridy = y++; c.gridwidth = 2; c.insets = new Insets(0, 0, 0, 0); add(panelMode, c); addKeyListener(ij); // ImageJ handles keyboard shortcuts pack(); GUI.center(this); setVisible(true); ip = setup(imp); if (ip == null) { imp.unlock(); IJ.beep(); IJ.showStatus("RGB image cannot be thresholded"); return; } thread.start(); }