/**
* Use the GUI results to set up the variables needed to run the algorithm.
*
* @return <code>true</code> if parameters set successfully, <code>false</code> otherwise.
*/
private boolean setVariables() {
String tmpStr;
int i;
int totLength = image.getExtents()[0];
for (i = 1; i < image.getNDims(); i++) {
totLength *= image.getExtents()[i];
}
tmpStr = greenMergingText.getText();
mergingDistance = Float.parseFloat(tmpStr);
if (mergingDistance < 0.0f) {
MipavUtil.displayError("Merging distance cannot be less than 0");
greenMergingText.requestFocus();
greenMergingText.selectAll();
return false;
}
tmpStr = redMinText.getText();
redMin = Integer.parseInt(tmpStr);
if (redMin < 1) {
MipavUtil.displayError("red minimum must be at least 1");
redMinText.requestFocus();
redMinText.selectAll();
return false;
} else if (redMin > totLength) {
MipavUtil.displayError("red minimum must not exceed " + totLength);
redMinText.requestFocus();
redMinText.selectAll();
return false;
}
tmpStr = redFractionText.getText();
redFraction = Float.parseFloat(tmpStr);
if (redFraction <= 0.0f) {
MipavUtil.displayError("red fraction must be greater than zero");
redFractionText.requestFocus();
redFractionText.selectAll();
return false;
} else if (redFraction > 1.0f) {
MipavUtil.displayError("red fraction must not exceed one");
redFractionText.requestFocus();
redFractionText.selectAll();
return false;
}
tmpStr = greenMinText.getText();
greenMin = Integer.parseInt(tmpStr);
if (greenMin < 1) {
MipavUtil.displayError("green minimum must be at least 1");
greenMinText.requestFocus();
greenMinText.selectAll();
return false;
} else if (greenMin > totLength) {
MipavUtil.displayError("green minimum must not exceed " + totLength);
greenMinText.requestFocus();
greenMinText.selectAll();
return false;
}
tmpStr = greenFractionText.getText();
greenFraction = Float.parseFloat(tmpStr);
if (greenFraction <= 0.0f) {
MipavUtil.displayError("green fraction must be greater than zero");
greenFractionText.requestFocus();
greenFractionText.selectAll();
return false;
} else if (greenFraction > 1.0f) {
MipavUtil.displayError("green fraction must not exceed one");
greenFractionText.requestFocus();
greenFractionText.selectAll();
return false;
}
tmpStr = blueMinText.getText();
blueMin = Integer.parseInt(tmpStr);
if (blueMin <= 0) {
MipavUtil.displayError("Number of blue pixels must be greater than 0");
blueMinText.requestFocus();
blueMinText.selectAll();
return false;
} else if (blueMin > totLength) {
MipavUtil.displayError("blue minimum must not exceed " + totLength);
blueMinText.requestFocus();
blueMinText.selectAll();
return false;
}
if (oneButton.isSelected()) {
greenRegionNumber = 1;
} else if (twoButton.isSelected()) {
greenRegionNumber = 2;
} else if (threeButton.isSelected()) {
greenRegionNumber = 3;
} else {
greenRegionNumber = 4;
}
twoGreenLevels = twoBox.isSelected();
tmpStr = blueValueText.getText();
blueBoundaryFraction = Float.parseFloat(tmpStr);
if (blueBoundaryFraction < 0.0f) {
MipavUtil.displayError("Blue boundary fraction cannot be less than 0.0");
blueValueText.requestFocus();
blueValueText.selectAll();
return false;
} else if (blueBoundaryFraction > 1.0f) {
MipavUtil.displayError("Blue boundary value cannot be greater than 1.0");
blueValueText.requestFocus();
blueValueText.selectAll();
return false;
}
blueSmooth = blueSmoothBox.isSelected();
tmpStr = interpolationText.getText();
interpolationDivisor = Float.parseFloat(tmpStr);
if (interpolationDivisor <= 1.0f) {
MipavUtil.displayError("Interpolation divisor must be greater than 1");
interpolationText.requestFocus();
interpolationText.selectAll();
return false;
}
return true;
} // end setVariables()
/** Sets up the GUI (panels, buttons, etc) and displays it on the screen. */
private void init() {
DecimalFormat df;
int xUnits;
String unitStr;
String distStr;
setForeground(Color.black);
setTitle("Center Distances version 2 07/14/08");
df = new DecimalFormat("0.000E0");
GridBagConstraints gbc = new GridBagConstraints();
int yPos = 0;
gbc.gridwidth = 1;
gbc.gridheight = 1;
gbc.anchor = GridBagConstraints.WEST;
gbc.weightx = 1;
gbc.insets = new Insets(3, 3, 3, 3);
gbc.fill = GridBagConstraints.HORIZONTAL;
gbc.gridx = 0;
gbc.gridy = yPos++;
JPanel mainPanel = new JPanel(new GridBagLayout());
mainPanel.setForeground(Color.black);
mainPanel.setBorder(buildTitledBorder("Input parameters"));
blueMinLabel = new JLabel("Minimum number of blue pixels per nucleus");
blueMinLabel.setForeground(Color.black);
blueMinLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(blueMinLabel, gbc);
blueMinText = new JTextField(5);
if (image.getNDims() == 2) {
blueMinText.setText("1000");
} else {
blueMinText.setText("20000");
}
blueMinText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(blueMinText, gbc);
redMinLabel = new JLabel("Minimum red pixel count");
redMinLabel.setForeground(Color.black);
redMinLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(redMinLabel, gbc);
redMinText = new JTextField(5);
redMinText.setText("50");
redMinText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(redMinText, gbc);
redFractionLabel = new JLabel("Fraction of red pixels to consider");
redFractionLabel.setForeground(Color.black);
redFractionLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(redFractionLabel, gbc);
redFractionText = new JTextField(5);
redFractionText.setText("0.15");
redFractionText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(redFractionText, gbc);
xUnits = image.getFileInfo(0).getUnitsOfMeasure()[0];
if (xUnits != Unit.UNKNOWN_MEASURE.getLegacyNum()) {
unitStr = (Unit.getUnitFromLegacyNum(xUnits)).toString();
greenMergingLabel = new JLabel("Green merging radius around peak (" + unitStr + ")");
} else {
greenMergingLabel = new JLabel("Green merging radius around peak");
}
greenMergingLabel.setForeground(Color.black);
greenMergingLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(greenMergingLabel, gbc);
if (image.getNDims() == 2) {
// mergingDistance = 8.0f * image.getFileInfo(0).getResolutions()[0];
mergingDistance = 0.0f;
} else {
// mergingDistance = 4.0f * image.getFileInfo(0).getResolutions()[0];
mergingDistance = 0.0f;
}
distStr = df.format(mergingDistance);
greenMergingText = new JTextField(10);
greenMergingText.setText(distStr);
greenMergingText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(greenMergingText, gbc);
greenMinLabel = new JLabel("Minimum green pixel count");
greenMinLabel.setForeground(Color.black);
greenMinLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(greenMinLabel, gbc);
greenMinText = new JTextField(5);
greenMinText.setText("10");
greenMinText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(greenMinText, gbc);
greenFractionLabel = new JLabel("Fraction of green pixels to consider");
greenFractionLabel.setForeground(Color.black);
greenFractionLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(greenFractionLabel, gbc);
greenFractionText = new JTextField(5);
greenFractionText.setText("0.01");
greenFractionText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(greenFractionText, gbc);
greenRegionsLabel = new JLabel("Green regions per cell");
greenRegionsLabel.setForeground(Color.black);
greenRegionsLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(greenRegionsLabel, gbc);
JPanel buttonPanel = new JPanel(new GridBagLayout());
greenGroup = new ButtonGroup();
oneButton = new JRadioButton("1", false);
oneButton.setForeground(Color.black);
oneButton.setFont(serif12);
greenGroup.add(oneButton);
gbc.gridx = 0;
gbc.gridy = 0;
buttonPanel.add(oneButton, gbc);
twoButton = new JRadioButton("2", true);
twoButton.setForeground(Color.black);
twoButton.setFont(serif12);
greenGroup.add(twoButton);
gbc.gridx = 1;
gbc.gridy = 0;
buttonPanel.add(twoButton, gbc);
threeButton = new JRadioButton("3", false);
threeButton.setForeground(Color.black);
threeButton.setFont(serif12);
greenGroup.add(threeButton);
gbc.gridx = 2;
gbc.gridy = 0;
buttonPanel.add(threeButton, gbc);
fourButton = new JRadioButton("4", false);
fourButton.setForeground(Color.black);
fourButton.setFont(serif12);
greenGroup.add(fourButton);
gbc.gridx = 3;
gbc.gridy = 0;
buttonPanel.add(fourButton, gbc);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(buttonPanel, gbc);
twoBox = new JCheckBox("Use 2 top gray levels in green segmentation", true);
twoBox.setForeground(Color.black);
twoBox.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos++;
mainPanel.add(twoBox, gbc);
blueValueLabel =
new JLabel("Fraction of blue transition from image min to max at nucleus boundary");
blueValueLabel.setForeground(Color.black);
blueValueLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(blueValueLabel, gbc);
blueValueText = new JTextField(5);
blueValueText.setText("0.15");
blueValueText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(blueValueText, gbc);
blueSmoothBox = new JCheckBox("Smooth blue VOI contours with AlgorithmBSmooth", true);
blueSmoothBox.setForeground(Color.black);
blueSmoothBox.setFont(serif12);
blueSmoothBox.addActionListener(this);
gbc.gridx = 0;
gbc.gridy = yPos++;
mainPanel.add(blueSmoothBox, gbc);
interpolationLabel = new JLabel("Number of interpolation points determined by divisor (> 1.0)");
interpolationLabel.setForeground(Color.black);
interpolationLabel.setFont(serif12);
gbc.gridx = 0;
gbc.gridy = yPos;
mainPanel.add(interpolationLabel, gbc);
interpolationText = new JTextField(5);
interpolationText.setText("24.0");
interpolationText.setFont(serif12);
gbc.gridx = 1;
gbc.gridy = yPos++;
mainPanel.add(interpolationText, gbc);
getContentPane().add(mainPanel, BorderLayout.CENTER);
getContentPane().add(buildButtons(), BorderLayout.SOUTH);
pack();
setVisible(true);
setResizable(false);
System.gc();
} // end init()