private String getDisplayName(SeqFeatureI sf) {
String display_name = "";
// Annot
if (sf instanceof AnnotatedFeatureI) {
display_name = sf.getName();
}
// Not Annot
else {
// FeatPair
FeatureSetI fset =
(sf.canHaveChildren() ? (FeatureSetI) sf : (FeatureSetI) sf.getRefFeature());
// cant do as seqfeature as getHitSequence() is in FeatureSet and not
// SeqFeature and furthermore cant do a getHitFeature().getRefSequence()
// as FeatureSet has no hit feature yet it has hit sequence
// SeqFeatureI fset = sf.canHaveChildren() ? sf : sf.getRefFeature();
SequenceI seq = (fset != null ? fset.getHitSequence() : null);
SeqFeatureI featPair = null; // FeaturePairI fp;
if (fset != null && fset.size() > 0 && (fset.getFeatureAt(0).hasHitFeature())) {
featPair = fset.getFeatureAt(0);
} else if (sf.hasHitFeature()) {
featPair = sf; // fp = (FeaturePairI) sf;
} // else {//fp = null; } // not necasary - already null
if (seq == null && featPair != null) seq = featPair.getHitFeature().getRefSequence();
if (seq == null) seq = sf.getFeatureSequence();
if (seq != null) {
display_name = seq.getName() != null ? seq.getName() : "";
} else if (featPair != null) {
SeqFeatureI hit = featPair.getHitFeature();
display_name = hit.getName() != null ? hit.getName() : "";
}
/* NOT FeaturePair
this will only be reached if not an annot, not a FS w fps
and not a FeaturePair (or if fp hit name is "")
in otherwords a seqfeature result
added getName in for chado gene predics which are leaf seq feats
*/
// shouldnt we check getName != null before using biotype??
else display_name = getBioTypeForDisplay(sf);
}
return display_name;
}
public CurationSet getCurationSet() throws apollo.dataadapter.ApolloAdapterException {
GbrowseAdapterGUI gui = (GbrowseAdapterGUI) getUI(ApolloDataAdapterI.OP_READ_DATA);
String chr = gui.getSelectedChr();
int chrstart = Integer.parseInt(gui.getSelectedStart());
int chrend = Integer.parseInt(gui.getSelectedEnd());
mysql = new MySQLInstance(host, user, pass, port);
db = mysql.fetchDatabaseByName(dbname);
dba = new DBAdaptor(db);
FeatureAdaptor fa = dba.getFeatureAdaptor();
FeatureSetI f = fa.fetchByReferenceStartEnd(chr, chrstart, chrend);
super.clearOldData();
CurationSet curationSet = new CurationSet();
curationSet.setChromosome(chr);
curationSet.setLow(chrstart);
curationSet.setHigh(chrend);
curationSet.setStrand(0);
StrandedFeatureSet sfset = new StrandedFeatureSet(new FeatureSet(), new FeatureSet());
for (int i = 0; i < f.size(); i++) {
SeqFeatureI sf = f.getFeatureAt(i);
sfset.addFeature(sf);
}
curationSet.setResults(sfset);
curationSet.setAnnots(new StrandedFeatureSet(new FeatureSet(), new FeatureSet()));
curationSet.setName(chr + "." + chrstart + "-" + chrend);
// super.notifyLoadingDone();
return curationSet;
}
/**
* Get component to be rendered, if pos outside of current range getFeatureAtPosition and reset
* currentRange, if feature is non null and not an instance of FeatureSetI then its an exon, and
* set isExon flag
*/
public Component getBaseComponent(int position, TierI tier, Orientation o) {
transcript = null;
int bp = tier.getBasePair(position);
int oldPos = position;
// We need to change the position if dealing with aa view
if (tier.getType() == SequenceType.AA) {
int offset = getOffset(position, tier);
bp += offset * tier.getStrand().toInt();
position = tier.getPosition(bp);
}
char base = tier.charAt(position);
if (tier.featureExsitsAt(position, TierI.Level.BOTTOM)) {
this.state = State.EXON;
SeqFeatureI feature = tier.featureAt(position, TierI.Level.BOTTOM);
Transcript parentClone = null;
Transcript otherParentClone = null;
if (tier.getType() == SequenceType.AA
&& feature.getParent() != null
&& feature.getParent().isTranscript()) {
FeatureSetI parent = feature.getParent();
int index = parent.getFeatureIndex(feature);
int start = parent.getEnd();
// the transcript start might be different than the lowest exon start
// when in the middle of an edit
if (parent.canHaveChildren()) {
for (int i = 0; i < parent.size(); i++) {
SeqFeatureI sf = parent.getFeatureAt(i);
if ((Strand.valueOf(sf.getStrand()) == Strand.FORWARD && sf.getStart() < start)
|| (Strand.valueOf(sf.getStrand()) == Strand.REVERSE && sf.getStart() > start))
start = sf.getStart();
}
}
int translationStart = parent.getTranslationStart();
int translationEnd = parent.getTranslationEnd();
int translationPos = parent.getFeaturePosition(translationStart);
int oldstart = start;
start += ((translationPos - 1) % 3) * parent.getStrand();
// Tring to avoid all the recalculation...
if (feature.getParent() == currentParent
&& start == currentStart
&& featureStart == feature.getStart()
&& featureEnd == feature.getEnd()) {
parentClone = currentParentClone;
otherParentClone = currentParentClone2;
} else {
// need to get a full translation so that we can get the amino acids
// in the UTR region
parentClone = (Transcript) feature.getParent().clone();
otherParentClone = (Transcript) feature.getParent().clone();
if (!parentClone.setTranslationStart(start)) {
start = oldstart;
TranslationI cds = otherParentClone.getTranslation();
cds.calcTranslationStartForLongestPeptide();
translationStart = otherParentClone.getTranslationStart();
translationPos = otherParentClone.getFeaturePosition(translationStart);
start += ((translationPos - 1) % 3) * otherParentClone.getStrand();
parentClone.setTranslationStart(start);
}
parentClone.setTranslationEnd(parentClone.getEnd());
parentClone.setPeptideSequence(null);
String translation = parentClone.translate();
SequenceI sequence = parentClone.getPeptideSequence();
currentParent = (Transcript) feature.getParent();
currentParentClone = parentClone;
currentParentClone2 = otherParentClone;
currentStart = start;
featureStart = feature.getStart();
featureEnd = feature.getEnd();
if (otherParentClone.getFeatureContaining(translationEnd) == null) {
TranslationI cds = otherParentClone.getTranslation();
cds.setTranslationEndFromStart();
}
}
SeqFeatureI sf = parentClone.getFeatureAt(index);
if (bp >= sf.getLow() && bp <= sf.getHigh()) {
base = getPeptide(bp, sf);
} else {
base = '\0';
}
if (otherParentClone != null) {
feature = otherParentClone.getFeatureAt(index);
}
} else if (tier.getType() == SequenceType.AA
&& feature.getParent() != null
&& !feature.getParent().isTranscript()) {
base = '\0';
}
if (isUTR(bp, feature, tier)) {
this.state = State.UTR;
}
if (isTranslationStart(bp, feature)) {
this.state = State.TRANSLATION_START;
}
if (isTranslationEnd(bp, feature)) {
this.state = State.TRANSLATION_END;
}
// Can have an error or a shift on a position but not both
if (isSequencingErrorPosition(bp, feature, tier.getType())) {
this.state = State.ERROR;
// set base to the base of the new sequence?
}
if (isShiftPosition(bp, feature, tier.getType())) {
this.state = State.SHIFT;
}
} else if (tier.featureExsitsAt(position, TierI.Level.TOP)) {
SeqFeatureI feature = tier.featureAt(position, Level.TOP);
this.state = State.INTRON;
if (tier.getType() == SequenceType.AA) {
base = '\0';
}
int start = feature.getEnd();
int end = feature.getStart();
if (feature.canHaveChildren()) {
for (int i = 0; i < feature.size(); i++) {
SeqFeatureI sf = feature.getFeatureAt(i);
if ((Strand.valueOf(sf.getStrand()) == Strand.FORWARD && sf.getStart() < start)
|| (Strand.valueOf(sf.getStrand()) == Strand.REVERSE && sf.getStart() > start))
start = sf.getStart();
if ((Strand.valueOf(sf.getStrand()) == Strand.FORWARD && sf.getEnd() > end)
|| (Strand.valueOf(sf.getStrand()) == Strand.REVERSE && sf.getEnd() < end))
end = sf.getEnd();
}
}
if (bp <= Math.min(start, end) || bp >= Math.max(start, end)) {
this.state = State.OUT;
base = '\0';
}
} else {
this.state = State.OUT;
base = '\0';
}
if (!(state == State.SHIFT || state == State.ERROR)
&& getRegionLow() <= position
&& position <= getRegionHigh()) {
this.state = State.SELECTED;
base = tier.charAt(oldPos);
}
init(base);
return this;
}
/**
* Display AnnotatedFeatureI feature. Exon, Transcript, and Gene are all GenericAnnotationI. No
* selection event is fired. (selectAnnot fires and displays)
*/
private void displayAnnot(AnnotatedFeatureI annot) {
currentAnnot = annot;
if (currentAnnot == null) {
transcriptComboBox.removeAllItems();
transcriptComboBox.addItem("<no feature selected>");
lengthLabel.setText("Translation length: <no feature selected>");
upstream_button.setLabel("");
downstream_button.setLabel("");
translationViewer.setTranscript(null, editorPanel.getSelectedTier()); // ??
return;
}
// else {
setupTranscriptComboBox(currentAnnot);
SeqFeatureI topAnnot = currentAnnot;
if (topAnnot.isTranscript()) topAnnot = currentAnnot.getRefFeature();
if (topAnnot.isProteinCodingGene()) {
String translation = currentAnnot.translate();
if (translation == null) {
lengthLabel.setText("Translation length: <no start selected>");
} else {
lengthLabel.setText("Translation length: " + currentAnnot.translate().length());
}
} else {
lengthLabel.setText(topAnnot.getFeatureType() + " annotation");
}
FeatureSetI holder = (FeatureSetI) topAnnot.getRefFeature();
neighbor_up = null;
neighbor_down = null;
if (holder != null) {
int index = holder.getFeatureIndex(topAnnot);
// get next neighbor up that has whole sequence
for (int i = index - 1; i >= 0 && neighbor_up == null; i--) {
FeatureSetI gene_sib = (FeatureSetI) holder.getFeatureAt(i);
if (gene_sib.getFeatureAt(0) instanceof Transcript) {
Transcript trans = (Transcript) gene_sib.getFeatureAt(0);
if (trans.haveWholeSequence()) // szap.getCurationSet()))
neighbor_up = trans;
}
}
// get next neighbor down that has whole sequence
for (int i = index + 1; i < holder.size() && neighbor_down == null; i++) {
FeatureSetI gene_sib = (FeatureSetI) holder.getFeatureAt(i);
if (gene_sib.getFeatureAt(0) instanceof Transcript) {
Transcript trans = (Transcript) gene_sib.getFeatureAt(0);
if (trans.haveWholeSequence()) // szap.getCurationSet()))
neighbor_down = trans;
}
}
}
upstream_button.setLabel(
neighbor_up == null
? ""
: "Go to next 5' annotation (" + neighbor_up.getParent().getName() + ")");
upstream_button.setVisible(neighbor_up != null);
downstream_button.setLabel(
neighbor_down == null
? ""
: "Go to next 3' annotation (" + neighbor_down.getParent().getName() + ")");
downstream_button.setVisible(neighbor_down != null);
// }
// todo - translationViewer take in 1 level annot
if (currentAnnot.isTranscript())
translationViewer.setTranscript((Transcript) currentAnnot, editorPanel.getSelectedTier());
}