Ejemplo n.º 1
0
  public static void main(String[] args)
      throws DependencyException, FormatException, IOException, ServiceException {
    if (args.length < 1) {
      System.out.println("Please specify a (small) image file.");
      System.exit(1);
    }
    String path = args[0];

    // read in entire file
    System.out.println("Reading file into memory from disk...");
    File inputFile = new File(path);
    int fileSize = (int) inputFile.length();
    DataInputStream in = new DataInputStream(new FileInputStream(inputFile));
    byte[] inBytes = new byte[fileSize];
    in.readFully(inBytes);
    System.out.println(fileSize + " bytes read.");

    // determine input file suffix
    String fileName = inputFile.getName();
    int dot = fileName.lastIndexOf(".");
    String suffix = dot < 0 ? "" : fileName.substring(dot);

    // map input id string to input byte array
    String inId = "inBytes" + suffix;
    Location.mapFile(inId, new ByteArrayHandle(inBytes));

    // read data from byte array using ImageReader
    System.out.println();
    System.out.println("Reading image data from memory...");

    ServiceFactory factory = new ServiceFactory();
    OMEXMLService service = factory.getInstance(OMEXMLService.class);
    IMetadata omeMeta = service.createOMEXMLMetadata();

    ImageReader reader = new ImageReader();
    reader.setMetadataStore(omeMeta);
    reader.setId(inId);
    int seriesCount = reader.getSeriesCount();
    int imageCount = reader.getImageCount();
    int sizeX = reader.getSizeX();
    int sizeY = reader.getSizeY();
    int sizeZ = reader.getSizeZ();
    int sizeC = reader.getSizeC();
    int sizeT = reader.getSizeT();

    // output some details
    System.out.println("Series count: " + seriesCount);
    System.out.println("First series:");
    System.out.println("\tImage count = " + imageCount);
    System.out.println("\tSizeX = " + sizeX);
    System.out.println("\tSizeY = " + sizeY);
    System.out.println("\tSizeZ = " + sizeZ);
    System.out.println("\tSizeC = " + sizeC);
    System.out.println("\tSizeT = " + sizeT);

    // map output id string to output byte array
    String outId = fileName + ".ome.tif";
    ByteArrayHandle outputFile = new ByteArrayHandle();
    Location.mapFile(outId, outputFile);

    // write data to byte array using ImageWriter
    System.out.println();
    System.out.print("Writing planes to destination in memory: ");
    ImageWriter writer = new ImageWriter();
    writer.setMetadataRetrieve(omeMeta);
    writer.setId(outId);

    byte[] plane = null;
    for (int i = 0; i < imageCount; i++) {
      if (plane == null) {
        // allow reader to allocate a new byte array
        plane = reader.openBytes(i);
      } else {
        // reuse previously allocated byte array
        reader.openBytes(i, plane);
      }
      writer.saveBytes(i, plane);
      System.out.print(".");
    }
    reader.close();
    writer.close();
    System.out.println();

    byte[] outBytes = outputFile.getBytes();
    outputFile.close();

    // flush output byte array to disk
    System.out.println();
    System.out.println("Flushing image data to disk...");
    File outFile = new File(fileName + ".ome.tif");
    DataOutputStream out = new DataOutputStream(new FileOutputStream(outFile));
    out.write(outBytes);
    out.close();
  }
  public static double[] readImageInfo(String id) {
    try {
      reader.setId(id);
    } catch (FormatException e) {
      // TODO Auto-generated catch block
      e.printStackTrace();
    } catch (IOException e) {
      // TODO Auto-generated catch block
      e.printStackTrace();
    }

    // ORDERING: 0 little, 1 seriesCount, 2 pixelType, 3 bpp, 4 itkComponentType, 5 sizeX,
    //           6 sizeY, 7 sizeZ, 8 sizeT, 9 sizeC, 10 effSizeC, 11 rgbChannelCount, 12 imageCount
    //           13 physX, 14 physY, 15 physZ, 16 timeIncrement
    double[] returnValues = new double[17];

    // return this and use SetByteOrderToLittleEndian or SetByteOrderToBigEndian in C++ land
    boolean little = reader.isLittleEndian();

    if (little) {
      returnValues[0] = 1;
    } else {
      returnValues[0] = 0;
    }

    returnValues[1] = reader.getSeriesCount();

    // return bpp and set an IOComponent based on it
    int pixelType = reader.getPixelType();
    returnValues[2] = (double) pixelType;
    returnValues[3] = (double) FormatTools.getBytesPerPixel((int) returnValues[2]);

    // 0 UCHAR, 1 CHAR, 2 USHORT, 3 SHORT, 4 UINT, 5 INT, 6 FLOAT, 7 DOUBLE, 8 UNKNOWN
    if (pixelType == FormatTools.UINT8) returnValues[4] = (double) 0;
    else if (pixelType == FormatTools.INT8) returnValues[4] = (double) 1;
    else if (pixelType == FormatTools.UINT16) returnValues[4] = (double) 2;
    else if (pixelType == FormatTools.INT16) returnValues[4] = (double) 3;
    else if (pixelType == FormatTools.UINT32) returnValues[4] = (double) 4;
    else if (pixelType == FormatTools.INT32) returnValues[4] = (double) 5;
    else if (pixelType == FormatTools.FLOAT) returnValues[4] = (double) 6;
    else if (pixelType == FormatTools.DOUBLE) returnValues[4] = (double) 7;
    else returnValues[4] = (double) 8;

    // return these
    returnValues[5] = (double) reader.getSizeX();
    returnValues[6] = (double) reader.getSizeY();
    returnValues[7] = (double) reader.getSizeZ();
    returnValues[8] = (double) reader.getSizeT();
    returnValues[9] = (double) reader.getSizeC();
    returnValues[10] = (double) reader.getEffectiveSizeC();
    returnValues[11] = (double) reader.getRGBChannelCount();
    returnValues[12] = (double) reader.getImageCount();

    MetadataRetrieve retrieve = MetadataTools.asRetrieve(reader.getMetadataStore());
    Double d = retrieve.getPixelsPhysicalSizeX(0).getValue();
    double d2 = d == null ? 1.0 : d.doubleValue();
    returnValues[13] = d2;
    d = retrieve.getPixelsPhysicalSizeY(0).getValue();
    d2 = d == null ? 1.0 : d.doubleValue();
    returnValues[14] = d2;
    d = retrieve.getPixelsPhysicalSizeZ(0).getValue();
    d2 = d == null ? 1.0 : d.doubleValue();
    returnValues[15] = d2;
    d = retrieve.getPixelsTimeIncrement(0);
    d2 = d == null ? 1.0 : d.doubleValue();
    returnValues[16] = d2;

    return returnValues;
  }