Ejemplo n.º 1
0
  // for segmented lines, not polygons
  public static double[] getSectors(float[] xxf, float[] yyf, double rad) {
    int len = xxf.length;
    double[] xx = new double[len];
    double[] yy = new double[len];
    for (int jj = 0; jj < xxf.length; jj++) {
      xx[jj] = xxf[jj];
      yy[jj] = yyf[jj];
    }
    double pi = Math.PI;
    double[] vAngles = getVertexAngles(xx, yy);
    double[] segAngles = getSegmentAngles(xx, yy);
    double phiSym, phiArc, phi;
    double dx, dy; // , px, py;
    for (int jj = 1; jj < len - 1; jj++) {

      phiSym = (segAngles[jj - 1] + segAngles[jj]) / 2;
      phiArc = 180 - vAngles[jj];
      int nPoints = 3;
      float[] arcPointsX = new float[nPoints + 1];
      float[] arcPointsY = new float[nPoints + 1];
      for (int pt = 0; pt < nPoints; pt++) {
        phi = phiSym - phiArc / 2 + pt * phiArc / (nPoints - 1);
        dx = rad * Math.cos(phi / 180 * pi);
        dy = rad * -Math.sin(phi / 180 * pi);
        arcPointsX[pt] = (float) (xx[jj] + dx);
        arcPointsY[pt] = (float) (yy[jj] + dy);
      }
      arcPointsX[nPoints] = (float) (xx[jj]);
      arcPointsY[nPoints] = (float) (yy[jj]);
      PolygonRoi polygon = new PolygonRoi(arcPointsX, arcPointsY, nPoints + 1, Roi.POLYGON);
      IJ.getImage().setRoi(polygon, true);
    }

    return null;
  }
Ejemplo n.º 2
0
  public static void main(String args[]) {
    // new ImageJ();
    IJ.open("/Applications/ImageJ/Bilder/Bear.jpg");
    // IJ.open("Z:/Pictures/Beispielbilder/orchid.jpg");

    GRDM_U2 pw = new GRDM_U2();
    pw.imp = IJ.getImage();
    pw.run("");
  }
Ejemplo n.º 3
0
 void hide() {
   ImagePlus imp = IJ.getImage();
   Overlay overlay = imp.getOverlay();
   if (overlay != null) {
     overlay2 = overlay;
     imp.setOverlay(null);
   }
   RoiManager rm = RoiManager.getInstance();
   if (rm != null) rm.runCommand("show none");
 }
Ejemplo n.º 4
0
  public boolean beadCalibration3d() {
    imp = IJ.getImage();
    if (imp == null) {
      IJ.noImage();
      return false;
    } else if (imp.getStackSize() == 1) {
      IJ.error("Stack required");
      return false;
    } else if (imp.getType() != ImagePlus.GRAY8 && imp.getType() != ImagePlus.GRAY16) {
      // In order to support 32bit images, pict[] must be changed to float[], and  getPixel(x, y);
      // requires a Float.intBitsToFloat() conversion
      IJ.error("8 or 16 bit greyscale image required");
      return false;
    }
    width = imp.getWidth();
    height = imp.getHeight();
    nslices = imp.getStackSize();
    imtitle = imp.getTitle();

    models[0] = "*None*";
    models[1] = "line";
    models[2] = "2nd degree polynomial";
    models[3] = "3rd degree polynomial";
    models[4] = "4th degree polynomial";

    GenericDialog gd = new GenericDialog("3D PALM calibration");
    gd.addNumericField("Maximum FWHM (in px)", prefs.get("QuickPALM.3Dcal_fwhm", 20), 0);
    gd.addNumericField(
        "Particle local threshold (% maximum intensity)", prefs.get("QuickPALM.pthrsh", 20), 0);
    gd.addNumericField("Z-spacing (nm)", prefs.get("QuickPALM.z-step", 10), 2);
    gd.addNumericField("Calibration Z-smoothing (radius)", prefs.get("QuickPALM.window", 1), 0);
    gd.addChoice("Model", models, prefs.get("QuickPALM.model", models[3]));
    gd.addCheckbox(
        "Show divergence of bead positions against model",
        prefs.get("QuickPALM.3Dcal_showDivergence", false));
    gd.addCheckbox("Show extra particle info", prefs.get("QuickPALM.3Dcal_showExtraInfo", false));
    gd.addMessage("\n\nDon't forget to save the table in the end...");
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    fwhm = gd.getNextNumber();
    prefs.set("QuickPALM.QuickPALM.3Dcal_fwhm", fwhm);
    pthrsh = gd.getNextNumber() / 100;
    prefs.set("QuickPALM.pthrsh", pthrsh * 100);
    cal_z = gd.getNextNumber();
    prefs.set("QuickPALM.z-step", cal_z);
    window = (int) gd.getNextNumber();
    prefs.set("QuickPALM.window", window);
    model = gd.getNextChoice();
    prefs.set("QuickPALM.model", model);
    part_divergence = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showDivergence", part_divergence);
    part_extrainfo = gd.getNextBoolean();
    prefs.set("QuickPALM.3Dcal_showExtraInfo", part_extrainfo);
    return true;
  }
Ejemplo n.º 5
0
  @Override
  public String commandExecuting(String command) {
    if (command.equalsIgnoreCase("Quit") && frame_ != null) {
      try {
        if (!frame_.closeSequence(true)) {
          return null;
        }
      } catch (Exception ex) {
        // do nothing, just make sure to continue quitting
      }
      return command;
    } else if (command.equals("Crop")) {
      if (IJ.getImage().getStack() instanceof AcquisitionVirtualStack) {

        new Duplicator().run(IJ.getImage()).show();

        // abort further processing of the Crop command
        return null;
      }
    } else if (command.equals("Add Noise")) {
      // blanket method to make sure that ImageJ filters do not execute on disk-cached images
      // this may backfire!
      if (IJ.getImage().getStack() instanceof AcquisitionVirtualStack) {
        AcquisitionVirtualStack avs = (AcquisitionVirtualStack) IJ.getImage().getStack();
        if (avs.getVirtualAcquisitionDisplay().isDiskCached()) {
          // duplicate the image and then run the ImageJ command on what is now the new image
          new Duplicator().run(IJ.getImage()).show();
        } else {
          // Image is not disk chached.  Warn that data will be lost
          if (!IJ.showMessageWithCancel(
              "Micro-Manager data not saved",
              "Data are not saved and Undo is impossible. \n"
                  + "Do you really want to execute the command?")) {
            return null;
          }
        }
      }
    }

    return command;
  }
Ejemplo n.º 6
0
  @Override
  public void run(final String arg) {
    imp = IJ.getImage();

    if (imp == null) {
      IJ.error("No image open.");
      return;
    }

    if (!setup(imp)) return;

    run(imp, width, height, minLambda, minPhi, hfov, vfov);
  }
Ejemplo n.º 7
0
 void addSelection() {
   ImagePlus imp = IJ.getImage();
   String macroOptions = Macro.getOptions();
   if (macroOptions != null && IJ.macroRunning() && macroOptions.indexOf("remove") != -1) {
     imp.setOverlay(null);
     return;
   }
   Roi roi = imp.getRoi();
   if (roi == null && imp.getOverlay() != null) {
     GenericDialog gd = new GenericDialog("No Selection");
     gd.addMessage("\"Overlay>Add\" requires a selection.");
     gd.setInsets(15, 40, 0);
     gd.addCheckbox("Remove existing overlay", false);
     gd.showDialog();
     if (gd.wasCanceled()) return;
     if (gd.getNextBoolean()) imp.setOverlay(null);
     return;
   }
   if (roi == null) {
     IJ.error("This command requires a selection.");
     return;
   }
   roi = (Roi) roi.clone();
   if (roi.getStrokeColor() == null) roi.setStrokeColor(Toolbar.getForegroundColor());
   int width = Line.getWidth();
   Rectangle bounds = roi.getBounds();
   boolean tooWide = width > Math.max(bounds.width, bounds.height) / 3.0;
   if (roi.getStroke() == null && width > 1 && !tooWide) roi.setStrokeWidth(Line.getWidth());
   Overlay overlay = imp.getOverlay();
   if (overlay != null && overlay.size() > 0 && !roi.isDrawingTool()) {
     Roi roi2 = overlay.get(overlay.size() - 1);
     if (roi.getStroke() == null) roi.setStrokeWidth(roi2.getStrokeWidth());
     if (roi.getFillColor() == null) roi.setFillColor(roi2.getFillColor());
   }
   boolean points = roi instanceof PointRoi && ((PolygonRoi) roi).getNCoordinates() > 1;
   if (points) roi.setStrokeColor(Color.red);
   if (!IJ.altKeyDown() && !(roi instanceof Arrow)) {
     RoiProperties rp = new RoiProperties("Add to Overlay", roi);
     if (!rp.showDialog()) return;
   }
   String name = roi.getName();
   boolean newOverlay = name != null && name.equals("new-overlay");
   if (overlay == null || newOverlay) overlay = new Overlay();
   overlay.add(roi);
   imp.setOverlay(overlay);
   overlay2 = overlay;
   if (points || (roi instanceof ImageRoi) || (roi instanceof Arrow)) imp.killRoi();
   Undo.setup(Undo.OVERLAY_ADDITION, imp);
 }
Ejemplo n.º 8
0
 public void run(String arg) {
   ImagePlus imp = IJ.getImage();
   int size = imp.getStackSize();
   if (size == 1) {
     IJ.error("Z Project", "This command requires a stack");
     return;
   }
   if (imp.isHyperStack()) {
     new ZProjector().run("");
     return;
   }
   if (!showDialog(imp)) return;
   ImagePlus imp2 = groupZProject(imp, method, groupSize);
   if (imp != null) imp2.show();
 }
Ejemplo n.º 9
0
 public void run(String arg) {
   imp = IJ.getImage();
   Roi roi = imp.getRoi();
   if (roi != null && !roi.isArea()) imp.killRoi(); // ignore any line selection
   ImageProcessor ip = imp.getProcessor();
   if (!showDialog(ip)) return;
   if (ip.getWidth() > 1 && ip.getHeight() > 1) ip.setInterpolate(interpolate);
   else ip.setInterpolate(false);
   ip.setBackgroundValue(bgValue);
   imp.startTiming();
   try {
     if (newWindow && imp.getStackSize() > 1 && processStack) createNewStack(imp, ip);
     else scale(ip);
   } catch (OutOfMemoryError o) {
     IJ.outOfMemory("Scale");
   }
   IJ.showProgress(1.0);
 }
Ejemplo n.º 10
0
 @Override
 public void run(String arg) {
   ImagePlus imp = IJ.getImage();
   GenericDialog gd = new GenericDialog(name);
   gd.addMessage("Requires SI raw data in OMX (CPZAT) order.");
   gd.addNumericField("angles", angles, 0);
   gd.addNumericField("phases", phases, 0);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   if (gd.wasOKed()) {
     angles = (int) gd.getNextNumber();
     phases = (int) gd.getNextNumber();
   }
   if (!I1l.stackDivisibleBy(imp, phases * angles)) {
     IJ.showMessage(name, "Error: stack size not consistent with phases/angles.");
     return;
   }
   results = exec(imp);
   results.report();
 }
Ejemplo n.º 11
0
 void fromRoiManager() {
   ImagePlus imp = IJ.getImage();
   RoiManager rm = RoiManager.getInstance();
   if (rm == null) {
     IJ.error("ROI Manager is not open");
     return;
   }
   Roi[] rois = rm.getRoisAsArray();
   if (rois.length == 0) {
     IJ.error("ROI Manager is empty");
     return;
   }
   Overlay overlay = new Overlay();
   for (int i = 0; i < rois.length; i++) overlay.add((Roi) rois[i].clone());
   imp.setOverlay(overlay);
   ImageCanvas ic = imp.getCanvas();
   if (ic != null) ic.setShowAllROIs(false);
   rm.setEditMode(imp, false);
   imp.killRoi();
 }
Ejemplo n.º 12
0
  public static void main(String[] args) {
    String mriImage = args[0];
    String outImage = args[1];
    String segArgs = args[2];
    String outTable = args[3];
    String pdxArgs = args[4];

    System.out.println("Open: " + mriImage);
    IJ.open(mriImage);
    COPD_LungSegment tjPlug = new COPD_LungSegment();
    ImagePlus cImage = IJ.getImage();
    tjPlug.setup(segArgs, cImage);
    tjPlug.run(cImage.getProcessor());
    IJ.save(cImage, outImage);
    COPD_PDxLAAx pdxPlug = new COPD_PDxLAAx();
    pdxPlug.setup("", cImage);
    pdxPlug.run(cImage.getProcessor());
    ResultsTable rt = Analyzer.getResultsTable();
    try {
      rt.saveAs(outTable);
    } catch (IOException e) {
      e.printStackTrace();
    }

    System.out.println("Results Table\n");
    String prefix = "rtout, ";

    for (int i = 0; i < ResultsTable.MAX_COLUMNS; i++) {
      if (!rt.columnExists(i)) break;
      String tempOut = rt.getColumnHeading(i);
      for (double d : rt.getColumnAsDoubles(i)) {
        tempOut += ", " + d;
      }
      System.out.println(prefix + tempOut);
    }
  }
Ejemplo n.º 13
0
 void toRoiManager() {
   ImagePlus imp = IJ.getImage();
   Overlay overlay = imp.getOverlay();
   if (overlay == null) {
     IJ.error("Overlay required");
     return;
   }
   RoiManager rm = RoiManager.getInstance();
   if (rm == null) {
     if (Macro.getOptions() != null && Interpreter.isBatchMode())
       rm = Interpreter.getBatchModeRoiManager();
     if (rm == null) {
       Frame frame = WindowManager.getFrame("ROI Manager");
       if (frame == null) IJ.run("ROI Manager...");
       frame = WindowManager.getFrame("ROI Manager");
       if (frame == null || !(frame instanceof RoiManager)) return;
       rm = (RoiManager) frame;
     }
   }
   rm.runCommand("reset");
   for (int i = 0; i < overlay.size(); i++) rm.add(imp, overlay.get(i), i);
   rm.setEditMode(imp, true);
   if (rm.getCount() == overlay.size()) imp.setOverlay(null);
 }
Ejemplo n.º 14
0
 public void run(String arg) {
   imp = IJ.getImage();
   if (imp == null) return;
   stackSize = imp.getStackSize();
   Roi roi = imp.getRoi();
   Calibration cal = imp.getCalibration();
   if (roi != null && roi.getBounds().equals(prevRoi) && cal.pixelWidth == prevPixelWidth)
     roi = null;
   if (roi != null) {
     boolean rectOrOval =
         roi != null && (roi.getType() == Roi.RECTANGLE || roi.getType() == Roi.OVAL);
     oval = rectOrOval && (roi.getType() == Roi.OVAL); // Handle existing oval ROI
     Rectangle r = roi.getBounds();
     width = r.width;
     height = r.height;
     xRoi = r.x;
     yRoi = r.y;
     if (scaledUnits && cal.scaled()) {
       xRoi = xRoi * cal.pixelWidth;
       yRoi = yRoi * cal.pixelHeight;
       width = width * cal.pixelWidth;
       height = height * cal.pixelHeight;
     }
     if (centered) { // Make xRoi and yRoi consistent when centered mode is active
       xRoi += width / 2.0;
       yRoi += height / 2.0;
     }
   } else if (!validDialogValues()) {
     width = imp.getWidth() / 2;
     height = imp.getHeight() / 2;
     xRoi = width / 2;
     yRoi = height / 2;
   }
   iSlice = imp.getCurrentSlice();
   showDialog();
 }
Ejemplo n.º 15
0
 void flatten() {
   ImagePlus imp = IJ.getImage();
   ImagePlus imp2 = imp.flatten();
   imp2.setTitle(WindowManager.getUniqueName(imp.getTitle()));
   imp2.show();
 }
Ejemplo n.º 16
0
 void show() {
   ImagePlus imp = IJ.getImage();
   if (overlay2 != null) imp.setOverlay(overlay2);
   RoiManager rm = RoiManager.getInstance();
   if (rm != null) rm.runCommand("show all");
 }
Ejemplo n.º 17
0
  // TODO split run method into more sensible methods
  public void run(final String arg) {
    if (!ImageCheck.checkEnvironment()) return;
    final ImagePlus imp = IJ.getImage();
    if (null == imp) {
      IJ.noImage();
      return;
    }
    final ImageCheck ic = new ImageCheck();
    if (!ImageCheck.isBinary(imp)) {
      IJ.showMessage("Fractal Count requires a binary image.");
      return;
    }
    if (noGo) return;
    final ImagePlus surfaceImp = findSurfaceVoxels(imp);
    try {
      // Fetch data
      final int width = imp.getWidth();
      final int height = imp.getHeight();
      final int depth = imp.getStackSize();

      if (autoParam) {
        maxBox = Math.max(width, Math.max(height, depth)) / autoDiv;
        if (verboseOutput) {
          IJ.log("Automatic max box size " + maxBox + " selected");
        }
      }

      // Create variables we need and set them
      long bestCount; // keep track of best count so far
      long count = 0; // current count
      final ArrayList<Double> xList = new ArrayList<Double>();
      final ArrayList<Double> yList = new ArrayList<Double>();
      int xPos, yPos, zPos, yPart;
      int xGrid, yGrid, zGrid;
      int xStart, yStart, zStart;
      int xEnd, yEnd, zEnd;

      // Start timer
      final long startTime = System.currentTimeMillis();

      for (int boxSize = maxBox; boxSize >= minBox; boxSize /= divBox) {
        if (verboseOutput) {
          IJ.showStatus("Estimating dimension, box size: " + boxSize);
        }

        bestCount = Long.MAX_VALUE; // init count for this boxSize

        final int increment = Math.max(1, boxSize / numOffsets);

        for (int gridOffsetX = 0;
            (gridOffsetX < boxSize) && (gridOffsetX < width);
            gridOffsetX += increment) {

          for (int gridOffsetY = 0;
              (gridOffsetY < boxSize) && (gridOffsetY < height);
              gridOffsetY += increment) {

            for (int gridOffsetZ = 0;
                (gridOffsetZ < boxSize) && (gridOffsetZ < depth);
                gridOffsetZ += increment) {

              count = 0;

              final int iMax = width + gridOffsetX;
              final int jMax = height + gridOffsetY;
              final int kMax = depth + gridOffsetZ;

              // Iterate over box-grid
              for (int i = 0; i <= iMax; i += boxSize) {
                xGrid = -gridOffsetX + i;
                for (int j = 0; j <= jMax; j += boxSize) {
                  yGrid = -gridOffsetY + j;
                  for (int k = 0; k <= kMax; k += boxSize) {
                    zGrid = -gridOffsetZ + k;

                    xStart = 0;
                    if (xGrid < 0) {
                      xStart = -xGrid;
                    }
                    if ((boxSize + xGrid) >= width) {
                      xEnd = Math.min(width, (width - xGrid));
                    } else {
                      xEnd = boxSize;
                    }

                    yStart = 0;
                    if (yGrid < 0) {
                      yStart = -yGrid;
                    }
                    if ((boxSize + yGrid) >= height) {
                      yEnd = Math.min(height, (height - yGrid));
                    } else {
                      yEnd = boxSize;
                    }

                    zStart = 0;
                    if (zGrid < 0) {
                      zStart = -zGrid;
                    }
                    if ((boxSize + zGrid) >= depth) {
                      zEnd = Math.min(depth, (depth - zGrid));
                    } else {
                      zEnd = boxSize;
                    }

                    for (int x = xStart; x < xEnd; x++) {
                      xPos = x + xGrid;

                      for (int y = yStart; y < yEnd; y++) {
                        yPos = (y + yGrid);
                        yPart = yPos * width;

                        for (int z = zStart; z < zEnd; z++) {
                          zPos = z + zGrid;

                          // If pixel inside region,
                          // count it

                          if ((0xff
                                  & ((byte[]) surfaceImp.getStack().getPixels(zPos + 1))
                                      [xPos + yPart])
                              >= threshold) {
                            count++;
                            z = x = y = boxSize; // stops
                            // things
                          }
                        }
                      }
                    }
                  }
                }
              }
              if (count < bestCount) {
                bestCount = count;
              }
            }
          }
        }
        xList.add(new Double(boxSize));
        yList.add(new Double(bestCount));
      }

      if (verboseOutput) {
        IJ.log("\nDuration: " + (System.currentTimeMillis() - startTime) / 1000.0 + " seconds\n");
      }

      final double[] boxSizes = new double[xList.size()];
      final double[] boxCountSums = new double[yList.size()];
      for (int i = 0; i < boxSizes.length; i++) {
        boxSizes[i] = -Math.log((xList.get(i)).doubleValue());
        boxCountSums[i] = Math.log((yList.get(i)).doubleValue());
      }

      if (verboseOutput) {
        IJ.log(
            "Used " + boxSizes.length + " different box sizes, from " + maxBox + " to " + minBox);
        IJ.log(
            "with a reduction rate of "
                + divBox
                + " and "
                + numOffsets
                + " translations of each box.");
      }

      if (boxSizes.length == 0) {
        IJ.error(
            "\nError: No boxes!\n"
                + "Make sure that starting and ending box size and"
                + "reduction rate allow for at least one"
                + " box size to exist!");
        return;
      }

      final CurveFitter cf = new CurveFitter(boxSizes, boxCountSums);
      cf.doFit(CurveFitter.STRAIGHT_LINE);
      final double[] p = cf.getParams();
      final double RSq = cf.getRSquared();

      if (verboseOutput) {
        IJ.log(
            imp.getTitle()
                + ": Dimension estimate: "
                + IJ.d2s(p[1], 4)
                + ": R²: "
                + RSq
                + ": Settings: "
                + maxBox
                + ":"
                + minBox
                + ":"
                + divBox
                + ":"
                + numOffsets);
      }

      if (verboseOutput) {
        IJ.log("Box Size    Box Count");
        for (int i = 0; i < boxSizes.length; i++) {
          IJ.log(IJ.d2s(xList.get(i), 0) + "            " + IJ.d2s(yList.get(i), 0));
        }
      }

      final ResultInserter ri = ResultInserter.getInstance();
      ri.setResultInRow(imp, "Fractal Dimension", p[1]);
      ri.setResultInRow(imp, "R²", RSq);
      ri.updateTable();

      if (plotGraph && !Interpreter.isBatchMode()) {
        drawGraph(p, boxSizes, boxCountSums);
      }
    } catch (final Exception e) {
      e.printStackTrace();
    }
    UsageReporter.reportEvent(this).send();
  }
Ejemplo n.º 18
0
  public void run(String arg) {
    ImageCheck ic = new ImageCheck();
    if (!ImageCheck.checkEnvironment()) return;
    ImagePlus imp = IJ.getImage();
    if (!ic.isBinary(imp)) {
      IJ.error("8-bit binary (black and white only) image required.");
      return;
    }

    if (!ic.isVoxelIsotropic(imp, 1E-3)) {
      if (IJ.showMessageWithCancel(
          "Anisotropic voxels",
          "This image contains anisotropic voxels, which will\n"
              + "result in incorrect thickness calculation.\n\n"
              + "Consider rescaling your data so that voxels are isotropic\n"
              + "(Image > Scale...).\n\n"
              + "Continue anyway?")) {
      } else return;
    }
    GenericDialog gd = new GenericDialog("Options");
    gd.addCheckbox("Thickness", true);
    gd.addCheckbox("Spacing", false);
    gd.addCheckbox("Graphic Result", true);
    gd.addCheckbox("Use_ROI_Manager", false);
    gd.addCheckbox("Mask thickness map", true);
    gd.addHelp("http://bonej.org/thickness");
    gd.showDialog();
    if (gd.wasCanceled()) {
      return;
    }
    boolean doThickness = gd.getNextBoolean();
    boolean doSpacing = gd.getNextBoolean();
    boolean doGraphic = gd.getNextBoolean();
    boolean doRoi = gd.getNextBoolean();
    boolean doMask = gd.getNextBoolean();

    long startTime = System.currentTimeMillis();
    String title = stripExtension(imp.getTitle());

    RoiManager roiMan = RoiManager.getInstance();
    // calculate trabecular thickness (Tb.Th)
    if (doThickness) {
      boolean inverse = false;
      ImagePlus impLTC = new ImagePlus();
      if (doRoi && roiMan != null) {
        ImageStack stack = RoiMan.cropStack(roiMan, imp.getStack(), true, 0, 1);
        ImagePlus crop = new ImagePlus(imp.getTitle(), stack);
        crop.setCalibration(imp.getCalibration());
        impLTC = getLocalThickness(crop, inverse, doMask);
      } else impLTC = getLocalThickness(imp, inverse, doMask);
      impLTC.setTitle(title + "_Tb.Th");
      impLTC.setCalibration(imp.getCalibration());
      double[] stats = StackStats.meanStdDev(impLTC);
      insertResults(imp, stats, inverse);
      if (doGraphic && !Interpreter.isBatchMode()) {
        impLTC.show();
        impLTC.setSlice(1);
        impLTC.getProcessor().setMinAndMax(0, stats[2]);
        IJ.run("Fire");
      }
    }
    if (doSpacing) {
      boolean inverse = true;
      ImagePlus impLTCi = new ImagePlus();
      if (doRoi && roiMan != null) {
        ImageStack stack = RoiMan.cropStack(roiMan, imp.getStack(), true, 255, 1);
        ImagePlus crop = new ImagePlus(imp.getTitle(), stack);
        crop.setCalibration(imp.getCalibration());
        impLTCi = getLocalThickness(crop, inverse, doMask);
      } else impLTCi = getLocalThickness(imp, inverse, doMask);
      // check marrow cavity size (i.e. trabcular separation, Tb.Sp)
      impLTCi.setTitle(title + "_Tb.Sp");
      impLTCi.setCalibration(imp.getCalibration());
      double[] stats = StackStats.meanStdDev(impLTCi);
      insertResults(imp, stats, inverse);
      if (doGraphic && !Interpreter.isBatchMode()) {
        impLTCi.show();
        impLTCi.setSlice(1);
        impLTCi.getProcessor().setMinAndMax(0, stats[2]);
        IJ.run("Fire");
      }
    }
    IJ.showProgress(1.0);
    IJ.showStatus("Done");
    double duration = ((double) System.currentTimeMillis() - (double) startTime) / (double) 1000;
    IJ.log("Duration = " + IJ.d2s(duration, 3) + " s");
    UsageReporter.reportEvent(this).send();
    return;
  }
  public void run(String arg) {
    int wOld, hOld, wNew, hNew;
    boolean fIsStack = false;

    ImagePlus imp = IJ.getImage();
    wOld = imp.getWidth();
    hOld = imp.getHeight();

    ImageStack stackOld = imp.getStack();
    if ((stackOld != null) && (stackOld.getSize() > 1)) fIsStack = true;

    String[] sPositions = {
      "Top-Left", "Top-Center", "Top-Right",
      "Center-Left", "Center", "Center-Right",
      "Bottom-Left", "Bottom-Center", "Bottom-Right"
    };

    String strTitle = fIsStack ? "Resize Stack Canvas" : "Resize Image Canvas";
    GenericDialog gd = new GenericDialog(strTitle);
    gd.addNumericField("Width:", wOld, 0, 5, "pixels");
    gd.addNumericField("Height:", hOld, 0, 5, "pixels");
    gd.addChoice("Position:", sPositions, sPositions[4]);
    gd.addCheckbox("Zero Fill", zeroFill);
    gd.showDialog();
    if (gd.wasCanceled()) return;

    wNew = (int) gd.getNextNumber();
    hNew = (int) gd.getNextNumber();
    int iPos = gd.getNextChoiceIndex();
    zeroFill = gd.getNextBoolean();
    Prefs.set("resizer.zero", zeroFill);

    int xOff, yOff;
    int xC = (wNew - wOld) / 2; // offset for centered
    int xR = (wNew - wOld); // offset for right
    int yC = (hNew - hOld) / 2; // offset for centered
    int yB = (hNew - hOld); // offset for bottom

    switch (iPos) {
      case 0: // TL
        xOff = 0;
        yOff = 0;
        break;
      case 1: // TC
        xOff = xC;
        yOff = 0;
        break;
      case 2: // TR
        xOff = xR;
        yOff = 0;
        break;
      case 3: // CL
        xOff = 0;
        yOff = yC;
        break;
      case 4: // C
        xOff = xC;
        yOff = yC;
        break;
      case 5: // CR
        xOff = xR;
        yOff = yC;
        break;
      case 6: // BL
        xOff = 0;
        yOff = yB;
        break;
      case 7: // BC
        xOff = xC;
        yOff = yB;
        break;
      case 8: // BR
        xOff = xR;
        yOff = yB;
        break;
      default: // center
        xOff = xC;
        yOff = yC;
        break;
    }

    if (fIsStack) {
      ImageStack stackNew = expandStack(stackOld, wNew, hNew, xOff, yOff);
      imp.setStack(null, stackNew);
    } else {
      if (!IJ.macroRunning()) Undo.setup(Undo.COMPOUND_FILTER, imp);
      ImageWindow win = imp.getWindow();
      if (win != null && (win instanceof PlotWindow)) ((PlotWindow) win).getPlot().setFrozen(true);
      ImageProcessor newIP = expandImage(imp.getProcessor(), wNew, hNew, xOff, yOff);
      imp.setProcessor(null, newIP);
      if (!IJ.macroRunning()) Undo.setup(Undo.COMPOUND_FILTER_DONE, imp);
    }
    Overlay overlay = imp.getOverlay();
    if (overlay != null) overlay.translate(xOff, yOff);
  }
Ejemplo n.º 20
0
 @Override
 public void run(String arg) {
   final ImagePlus imp = IJ.getImage();
   run(IJ.getImage().getProcessor(), 0);
   imp.updateAndDraw();
 }
Ejemplo n.º 21
0
  /** Ask for parameters and then execute. */
  public void run(String arg) {
    // 1 - Obtain the currently active image:
    ImagePlus imp = IJ.getImage();

    if (null == imp) {
      IJ.showMessage("There must be at least one image open");
      return;
    }

    if (imp.getBitDepth() != 8) {
      IJ.showMessage("Error", "Only 8-bit images are supported");
      return;
    }

    // 2 - Ask for parameters:
    GenericDialog gd = new GenericDialog("Auto Local Threshold");
    String[] methods = {
      "Try all",
      "Bernsen",
      "Contrast",
      "Mean",
      "Median",
      "MidGrey",
      "Niblack",
      "Otsu",
      "Phansalkar",
      "Sauvola"
    };
    gd.addMessage("Auto Local Threshold v1.5");
    gd.addChoice("Method", methods, methods[0]);
    gd.addNumericField("Radius", 15, 0);
    gd.addMessage("Special paramters (if different from default)");
    gd.addNumericField("Parameter_1", 0, 0);
    gd.addNumericField("Parameter_2", 0, 0);
    gd.addCheckbox("White objects on black background", true);
    if (imp.getStackSize() > 1) {
      gd.addCheckbox("Stack", false);
    }
    gd.addMessage("Thresholded result is always shown in white [255].");
    gd.showDialog();
    if (gd.wasCanceled()) return;

    // 3 - Retrieve parameters from the dialog
    String myMethod = gd.getNextChoice();
    int radius = (int) gd.getNextNumber();
    double par1 = (double) gd.getNextNumber();
    double par2 = (double) gd.getNextNumber();
    boolean doIwhite = gd.getNextBoolean();
    boolean doIstack = false;

    int stackSize = imp.getStackSize();
    if (stackSize > 1) doIstack = gd.getNextBoolean();

    // 4 - Execute!
    // long start = System.currentTimeMillis();
    if (myMethod.equals("Try all")) {
      ImageProcessor ip = imp.getProcessor();
      int xe = ip.getWidth();
      int ye = ip.getHeight();
      int ml = methods.length;
      ImagePlus imp2, imp3;
      ImageStack tstack = null, stackNew;
      if (stackSize > 1 && doIstack) {
        boolean doItAnyway = true;
        if (stackSize > 25) {
          YesNoCancelDialog d =
              new YesNoCancelDialog(
                  IJ.getInstance(),
                  "Auto Local Threshold",
                  "You might run out of memory.\n \nDisplay "
                      + stackSize
                      + " slices?\n \n \'No\' will process without display and\noutput results to the log window.");
          if (!d.yesPressed()) {
            //						doIlog=true; //will show in the log window
            doItAnyway = false;
          }
          if (d.cancelPressed()) return;
        }

        for (int j = 1; j <= stackSize; j++) {
          imp.setSlice(j);
          ip = imp.getProcessor();
          tstack = new ImageStack(xe, ye);
          for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
          imp2 = new ImagePlus("Auto Threshold", tstack);
          imp2.updateAndDraw();

          for (int k = 1; k < ml; k++) {
            imp2.setSlice(k);
            Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
          }
          // if (doItAnyway){
          CanvasResizer cr = new CanvasResizer();
          stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
          imp3 = new ImagePlus("Auto Threshold", stackNew);
          imp3.updateAndDraw();
          MontageMaker mm = new MontageMaker();
          mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true); // 3 columns and 3 rows
        }
        imp.setSlice(1);
        // if (doItAnyway)
        IJ.run("Images to Stack", "method=[Copy (center)] title=Montage");
        return;
      } else { // single image try all
        tstack = new ImageStack(xe, ye);
        for (int k = 1; k < ml; k++) tstack.addSlice(methods[k], ip.duplicate());
        imp2 = new ImagePlus("Auto Threshold", tstack);
        imp2.updateAndDraw();

        for (int k = 1; k < ml; k++) {
          imp2.setSlice(k);
          // IJ.log("analyzing slice with "+methods[k]);
          Object[] result = exec(imp2, methods[k], radius, par1, par2, doIwhite);
        }
        // imp2.setSlice(1);
        CanvasResizer cr = new CanvasResizer();
        stackNew = cr.expandStack(tstack, (xe + 2), (ye + 18), 1, 1);
        imp3 = new ImagePlus("Auto Threshold", stackNew);
        imp3.updateAndDraw();
        MontageMaker mm = new MontageMaker();
        mm.makeMontage(imp3, 3, 3, 1.0, 1, (ml - 1), 1, 0, true);
        return;
      }
    } else { // selected a method
      if (stackSize > 1 && doIstack) { // whole stack
        //				if (doIstackHistogram) {// one global histogram
        //					Object[] result = exec(imp, myMethod, noWhite, noBlack, doIwhite, doIset, doIlog,
        // doIstackHistogram );
        //				}
        //				else{ // slice by slice
        for (int k = 1; k <= stackSize; k++) {
          imp.setSlice(k);
          Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
        }
        //				}
        imp.setSlice(1);
      } else { // just one slice
        Object[] result = exec(imp, myMethod, radius, par1, par2, doIwhite);
      }
      // 5 - If all went well, show the image:
      // not needed here as the source image is binarised
    }
  }
Ejemplo n.º 22
0
  void addImage() {
    ImagePlus imp = IJ.getImage();
    int[] wList = WindowManager.getIDList();
    if (wList == null || wList.length < 2) {
      IJ.error("Add Image...", "The command requires at least two open images.");
      return;
    }
    String[] titles = new String[wList.length];
    for (int i = 0; i < wList.length; i++) {
      ImagePlus imp2 = WindowManager.getImage(wList[i]);
      titles[i] = imp2 != null ? imp2.getTitle() : "";
    }
    int x = 0, y = 0;
    Roi roi = imp.getRoi();
    if (roi != null && roi.isArea()) {
      Rectangle r = roi.getBounds();
      x = r.x;
      y = r.y;
    }
    int index = 0;
    if (wList.length == 2) {
      ImagePlus i1 = WindowManager.getImage(wList[0]);
      ImagePlus i2 = WindowManager.getImage(wList[1]);
      if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) index = 1;
    } else if (imp.getID() == wList[0]) index = 1;

    GenericDialog gd = new GenericDialog("Add Image...");
    gd.addChoice("Image to add:", titles, titles[index]);
    gd.addNumericField("X location:", x, 0);
    gd.addNumericField("Y location:", y, 0);
    gd.addNumericField("Opacity (0-100%):", 100, 0);
    gd.addCheckbox("Create image selection", createImageRoi);
    gd.showDialog();
    if (gd.wasCanceled()) return;
    index = gd.getNextChoiceIndex();
    x = (int) gd.getNextNumber();
    y = (int) gd.getNextNumber();
    double opacity = gd.getNextNumber() / 100.0;
    createImageRoi = gd.getNextBoolean();
    ImagePlus overlay = WindowManager.getImage(wList[index]);
    if (wList.length == 2) {
      ImagePlus i1 = WindowManager.getImage(wList[0]);
      ImagePlus i2 = WindowManager.getImage(wList[1]);
      if (i2.getWidth() < i1.getWidth() && i2.getHeight() < i1.getHeight()) {
        imp = i1;
        overlay = i2;
      }
    }
    if (overlay == imp) {
      IJ.error(
          "Add Image...", "Image to be added cannot be the same as\n\"" + imp.getTitle() + "\".");
      return;
    }
    if (overlay.getWidth() > imp.getWidth() && overlay.getHeight() > imp.getHeight()) {
      IJ.error(
          "Add Image...", "Image to be added cannnot be larger than\n\"" + imp.getTitle() + "\".");
      return;
    }
    if (createImageRoi && x == 0 && y == 0) {
      x = imp.getWidth() / 2 - overlay.getWidth() / 2;
      y = imp.getHeight() / 2 - overlay.getHeight() / 2;
    }
    roi = new ImageRoi(x, y, overlay.getProcessor());
    roi.setName(overlay.getShortTitle());
    if (opacity != 1.0) ((ImageRoi) roi).setOpacity(opacity);
    if (createImageRoi) imp.setRoi(roi);
    else {
      Overlay overlayList = imp.getOverlay();
      if (overlayList == null) overlayList = new Overlay();
      overlayList.add(roi);
      imp.setOverlay(overlayList);
      overlay2 = overlayList;
      Undo.setup(Undo.OVERLAY_ADDITION, imp);
    }
  }
Ejemplo n.º 23
0
 public void run(String arg) {
   imp = IJ.getImage();
   showDialog();
 }
Ejemplo n.º 24
0
  public void run(String arg) {
    imp = IJ.getImage();
    int stackSize = imp.getStackSize();
    if (imp == null) {
      IJ.noImage();
      return;
    }

    //  Make sure input image is a stack.
    if (stackSize == 1) {
      IJ.error("Z Project", "Stack required");
      return;
    }

    //  Check for inverting LUT.
    if (imp.getProcessor().isInvertedLut()) {
      if (!IJ.showMessageWithCancel("ZProjection", lutMessage)) return;
    }

    // Set default bounds.
    int channels = imp.getNChannels();
    int frames = imp.getNFrames();
    int slices = imp.getNSlices();
    isHyperstack =
        imp.isHyperStack()
            || (ij.macro.Interpreter.isBatchMode()
                && ((frames > 1 && frames < stackSize) || (slices > 1 && slices < stackSize)));
    boolean simpleComposite = channels == stackSize;
    if (simpleComposite) isHyperstack = false;
    startSlice = 1;
    if (isHyperstack) {
      int nSlices = imp.getNSlices();
      if (nSlices > 1) stopSlice = nSlices;
      else stopSlice = imp.getNFrames();
    } else stopSlice = stackSize;

    // Build control dialog
    GenericDialog gd = buildControlDialog(startSlice, stopSlice);
    gd.showDialog();
    if (gd.wasCanceled()) return;

    if (!imp.lock()) return; // exit if in use
    long tstart = System.currentTimeMillis();
    setStartSlice((int) gd.getNextNumber());
    setStopSlice((int) gd.getNextNumber());
    method = gd.getNextChoiceIndex();
    Prefs.set(METHOD_KEY, method);
    if (isHyperstack) {
      allTimeFrames = imp.getNFrames() > 1 && imp.getNSlices() > 1 ? gd.getNextBoolean() : false;
      doHyperStackProjection(allTimeFrames);
    } else if (imp.getType() == ImagePlus.COLOR_RGB) doRGBProjection(true);
    else doProjection(true);

    if (arg.equals("") && projImage != null) {
      long tstop = System.currentTimeMillis();
      projImage.setCalibration(imp.getCalibration());
      if (simpleComposite) IJ.run(projImage, "Grays", "");
      projImage.show("ZProjector: " + IJ.d2s((tstop - tstart) / 1000.0, 2) + " seconds");
    }

    imp.unlock();
    IJ.register(ZProjector.class);
    return;
  }