Ejemplo n.º 1
0
 void addToRoiManager(ImagePlus imp) {
   if (IJ.macroRunning() && Interpreter.isBatchModeRoiManager())
     IJ.error("run(\"Add to Manager\") may not work in batch mode macros");
   Frame frame = WindowManager.getFrame("ROI Manager");
   if (frame == null) IJ.run("ROI Manager...");
   if (imp == null) return;
   Roi roi = imp.getRoi();
   if (roi == null) return;
   frame = WindowManager.getFrame("ROI Manager");
   if (frame == null || !(frame instanceof RoiManager)) IJ.error("ROI Manager not found");
   RoiManager rm = (RoiManager) frame;
   boolean altDown = IJ.altKeyDown();
   IJ.setKeyUp(IJ.ALL_KEYS);
   if (altDown && !IJ.macroRunning()) IJ.setKeyDown(KeyEvent.VK_SHIFT);
   if (roi.getState() == Roi.CONSTRUCTING) { // wait (up to 2 sec.) until ROI finished
     long start = System.currentTimeMillis();
     while (true) {
       IJ.wait(10);
       if (roi.getState() != Roi.CONSTRUCTING) break;
       if ((System.currentTimeMillis() - start) > 2000) {
         IJ.beep();
         IJ.error("Add to Manager", "Selection is not complete");
         return;
       }
     }
   }
   rm.runCommand("add");
   IJ.setKeyUp(IJ.ALL_KEYS);
 }
 /**
  * Saves statistics for one particle in a results table. This is a method subclasses may want to
  * override.
  */
 protected void saveResults(ImageStatistics stats, Roi roi) {
   analyzer.saveResults(stats, roi);
   if (recordStarts) {
     rt.addValue("XStart", stats.xstart);
     rt.addValue("YStart", stats.ystart);
   }
   if (addToManager) {
     if (roiManager == null) {
       if (Macro.getOptions() != null && Interpreter.isBatchMode())
         roiManager = Interpreter.getBatchModeRoiManager();
       if (roiManager == null) {
         Frame frame = WindowManager.getFrame("ROI Manager");
         if (frame == null) IJ.run("ROI Manager...");
         frame = WindowManager.getFrame("ROI Manager");
         if (frame == null || !(frame instanceof RoiManager)) {
           addToManager = false;
           return;
         }
         roiManager = (RoiManager) frame;
       }
       if (resetCounter) roiManager.runCommand("reset");
     }
     if (imp.getStackSize() > 1) roi.setPosition(imp.getCurrentSlice());
     if (lineWidth != 1) roi.setStrokeWidth(lineWidth);
     roiManager.add(imp, roi, rt.getCounter());
   }
   if (showResults) rt.addResults();
 }
  public String[] getOpenImageNames() {
    int[] imageIDList = WindowManager.getIDList();
    String[] imageNames = new String[imageIDList.length];
    // imageNames[0] = "None";

    if (!imageIDList.equals(null) && imageIDList.length > 1) {
      for (int i = 0; i < imageIDList.length; i++) {
        ImagePlus tempIMP = WindowManager.getImage(imageIDList[i]);
        imageNames[i] = tempIMP.getTitle();
      }
      return imageNames;
    } else {
      imageNames[0] = "None";
      return imageNames;
    }
  }
Ejemplo n.º 4
0
 public void run(String arg) {
   imp = WindowManager.getCurrentImage();
   if (arg.equals("add")) {
     addToRoiManager(imp);
     return;
   }
   if (imp == null) {
     IJ.noImage();
     return;
   }
   if (arg.equals("all")) imp.setRoi(0, 0, imp.getWidth(), imp.getHeight());
   else if (arg.equals("none")) imp.killRoi();
   else if (arg.equals("restore")) imp.restoreRoi();
   else if (arg.equals("spline")) fitSpline();
   else if (arg.equals("circle")) fitCircle(imp);
   else if (arg.equals("ellipse")) createEllipse(imp);
   else if (arg.equals("hull")) convexHull(imp);
   else if (arg.equals("mask")) createMask(imp);
   else if (arg.equals("from")) createSelectionFromMask(imp);
   else if (arg.equals("inverse")) invert(imp);
   else if (arg.equals("toarea")) lineToArea(imp);
   else if (arg.equals("toline")) areaToLine(imp);
   else if (arg.equals("properties")) {
     setProperties("Properties ", imp.getRoi());
     imp.draw();
   } else if (arg.equals("band")) makeBand(imp);
   else if (arg.equals("tobox")) toBoundingBox(imp);
   else runMacro(arg);
 }
Ejemplo n.º 5
0
 void createMask(ImagePlus imp) {
   Roi roi = imp.getRoi();
   boolean useInvertingLut = Prefs.useInvertingLut;
   Prefs.useInvertingLut = false;
   if (roi == null || !(roi.isArea() || roi.getType() == Roi.POINT)) {
     createMaskFromThreshold(imp);
     Prefs.useInvertingLut = useInvertingLut;
     return;
   }
   ImagePlus maskImp = null;
   Frame frame = WindowManager.getFrame("Mask");
   if (frame != null && (frame instanceof ImageWindow))
     maskImp = ((ImageWindow) frame).getImagePlus();
   if (maskImp == null) {
     ImageProcessor ip = new ByteProcessor(imp.getWidth(), imp.getHeight());
     if (!Prefs.blackBackground) ip.invertLut();
     maskImp = new ImagePlus("Mask", ip);
     maskImp.show();
   }
   ImageProcessor ip = maskImp.getProcessor();
   ip.setRoi(roi);
   ip.setValue(255);
   ip.fill(ip.getMask());
   maskImp.updateAndDraw();
   Prefs.useInvertingLut = useInvertingLut;
 }
 void updateSliceSummary() {
   int slices = imp.getStackSize();
   float[] areas = rt.getColumn(ResultsTable.AREA);
   if (areas == null) areas = new float[0];
   String label = imp.getTitle();
   if (slices > 1) {
     label = imp.getStack().getShortSliceLabel(slice);
     label = label != null && !label.equals("") ? label : "" + slice;
   }
   String aLine = null;
   double sum = 0.0;
   int start = areas.length - particleCount;
   if (start < 0) return;
   for (int i = start; i < areas.length; i++) sum += areas[i];
   int places = Analyzer.getPrecision();
   Calibration cal = imp.getCalibration();
   String total = "\t" + ResultsTable.d2s(sum, places);
   String average = "\t" + ResultsTable.d2s(sum / particleCount, places);
   String fraction = "\t" + ResultsTable.d2s(sum * 100.0 / totalArea, 1);
   aLine = label + "\t" + particleCount + total + average + fraction;
   aLine = addMeans(aLine, areas.length > 0 ? start : -1);
   if (slices == 1) {
     Frame frame = WindowManager.getFrame("Summary");
     if (frame != null && (frame instanceof TextWindow) && summaryHdr.equals(prevHdr))
       tw = (TextWindow) frame;
   }
   if (tw == null) {
     String title = slices == 1 ? "Summary" : "Summary of " + imp.getTitle();
     tw = new TextWindow(title, summaryHdr, aLine, 450, 300);
     prevHdr = summaryHdr;
   } else tw.append(aLine);
 }
Ejemplo n.º 7
0
 void addToRoiManager(ImagePlus imp) {
   if (IJ.macroRunning() && Interpreter.isBatchModeRoiManager())
     IJ.error("run(\"Add to Manager\") may not work in batch mode macros");
   Frame frame = WindowManager.getFrame("ROI Manager");
   if (frame == null) IJ.run("ROI Manager...");
   if (imp == null) return;
   Roi roi = imp.getRoi();
   if (roi == null) return;
   frame = WindowManager.getFrame("ROI Manager");
   if (frame == null || !(frame instanceof RoiManager)) IJ.error("ROI Manager not found");
   RoiManager rm = (RoiManager) frame;
   boolean altDown = IJ.altKeyDown();
   IJ.setKeyUp(IJ.ALL_KEYS);
   if (altDown && !IJ.macroRunning()) IJ.setKeyDown(KeyEvent.VK_SHIFT);
   rm.runCommand("add");
   IJ.setKeyUp(IJ.ALL_KEYS);
 }
Ejemplo n.º 8
0
 boolean setProperties(String title, Roi roi) {
   Frame f = WindowManager.getFrontWindow();
   if (f != null && f.getTitle().indexOf("3D Viewer") != -1) return false;
   if (roi == null) {
     IJ.error("This command requires a selection.");
     return false;
   }
   RoiProperties rp = new RoiProperties(title, roi);
   return rp.showDialog();
 }
Ejemplo n.º 9
0
 /** Sets the color used used for the ROI Manager "Show All" mode. */
 public static void setShowAllColor(Color c) {
   if (c == null) return;
   showAllColor = c;
   labelColor = null;
   ImagePlus img = WindowManager.getCurrentImage();
   if (img != null) {
     ImageCanvas ic = img.getCanvas();
     if (ic != null && ic.getShowAllROIs()) img.draw();
   }
 }
Ejemplo n.º 10
0
 private boolean checkImage() {
   imp = WindowManager.getCurrentImage();
   if (imp == null) {
     IJ.beep();
     IJ.showStatus("No image");
     return false;
   }
   ip = setup(imp);
   if (ip == null) return false;
   return true;
 }
Ejemplo n.º 11
0
 void createMaskFromThreshold(ImagePlus imp) {
   ImageProcessor ip = imp.getProcessor();
   if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) {
     IJ.error("Create Mask", "Area selection or thresholded image required");
     return;
   }
   double t1 = ip.getMinThreshold();
   double t2 = ip.getMaxThreshold();
   IJ.run("Duplicate...", "title=mask");
   ImagePlus imp2 = WindowManager.getCurrentImage();
   ImageProcessor ip2 = imp2.getProcessor();
   ip2.setThreshold(t1, t2, ImageProcessor.NO_LUT_UPDATE);
   IJ.run("Convert to Mask");
 }
Ejemplo n.º 12
0
 void checkForCalibrationConflict(ImagePlus imp, Calibration cal) {
   Calibration gcal = imp.getGlobalCalibration();
   if (gcal == null || !showConflictMessage || IJ.isMacro()) return;
   if (cal.pixelWidth == gcal.pixelWidth && cal.getUnit().equals(gcal.getUnit())) return;
   GenericDialog gd = new GenericDialog(imp.getTitle());
   gd.addMessage("The calibration of this image conflicts\nwith the current global calibration.");
   gd.addCheckbox("Disable_Global Calibration", true);
   gd.addCheckbox("Disable_these Messages", false);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   boolean disable = gd.getNextBoolean();
   if (disable) {
     imp.setGlobalCalibration(null);
     imp.setCalibration(cal);
     WindowManager.repaintImageWindows();
   }
   boolean dontShow = gd.getNextBoolean();
   if (dontShow) showConflictMessage = false;
 }
Ejemplo n.º 13
0
 void createMask(ImagePlus imp) {
   Roi roi = imp.getRoi();
   boolean useInvertingLut = Prefs.useInvertingLut;
   Prefs.useInvertingLut = false;
   boolean selectAll =
       roi != null
           && roi.getType() == Roi.RECTANGLE
           && roi.getBounds().width == imp.getWidth()
           && roi.getBounds().height == imp.getHeight()
           && imp.isThreshold();
   if (roi == null || !(roi.isArea() || roi.getType() == Roi.POINT) || selectAll) {
     createMaskFromThreshold(imp);
     Prefs.useInvertingLut = useInvertingLut;
     return;
   }
   ImagePlus maskImp = null;
   Frame frame = WindowManager.getFrame("Mask");
   if (frame != null && (frame instanceof ImageWindow))
     maskImp = ((ImageWindow) frame).getImagePlus();
   if (maskImp == null) {
     ImageProcessor ip = new ByteProcessor(imp.getWidth(), imp.getHeight());
     if (!Prefs.blackBackground) ip.invertLut();
     maskImp = new ImagePlus("Mask", ip);
     maskImp.show();
   }
   ImageProcessor ip = maskImp.getProcessor();
   ip.setRoi(roi);
   ip.setValue(255);
   ip.fill(ip.getMask());
   Calibration cal = imp.getCalibration();
   if (cal.scaled()) {
     Calibration cal2 = maskImp.getCalibration();
     cal2.pixelWidth = cal.pixelWidth;
     cal2.pixelHeight = cal.pixelHeight;
     cal2.setUnit(cal.getUnit());
   }
   maskImp.updateAndRepaintWindow();
   Prefs.useInvertingLut = useInvertingLut;
 }
Ejemplo n.º 14
0
  public void run(String arg) {
    ImagePlus imp = WindowManager.getCurrentImage();
    Calibration cal = imp.getCalibration();
    GenericDialog gd = new GenericDialog("Options");
    int subsize = 32;
    gd.addNumericField("Subregion Size (pixels)?", subsize, 0);
    int stepsize = 16;
    gd.addNumericField("Step Size?", stepsize, 0);
    int shift = 3;
    gd.addNumericField("STICS temporal Shift?", shift, 0);
    float xoffset = 0.0f;
    gd.addNumericField("X_Offset", xoffset, 5, 15, null);
    float yoffset = 0.0f;
    gd.addNumericField("Y_Offset", yoffset, 5, 15, null);
    float multiplier = 8.0f;
    gd.addNumericField("Velocity Multiplier", multiplier, 5, 15, null);
    float ftime = 1.0f;
    gd.addNumericField("Frame_Time(min)", ftime, 5, 15, null);
    float scaling = (float) cal.pixelWidth;
    gd.addNumericField("Pixel_Size(um)", scaling, 5, 15, null);
    boolean norm = true;
    gd.addCheckbox("Normalize_Vector_lengths?", norm);
    boolean centered = true;
    gd.addCheckbox("Center_Vectors?", centered);
    float magthresh = 0.0f;
    gd.addNumericField("Magnitude_Threshhold?", magthresh, 5, 15, null);
    int rlength = 10;
    gd.addNumericField("Running_avg_length", rlength, 0);
    int inc = 5;
    gd.addNumericField("Start_frame_increment", inc, 0);
    gd.showDialog();
    if (gd.wasCanceled()) {
      return;
    }
    subsize = (int) gd.getNextNumber();
    stepsize = (int) gd.getNextNumber();
    shift = (int) gd.getNextNumber();
    xoffset = (float) gd.getNextNumber();
    yoffset = (float) gd.getNextNumber();
    multiplier = (float) gd.getNextNumber();
    ftime = (float) gd.getNextNumber();
    scaling = (float) gd.getNextNumber();
    norm = gd.getNextBoolean();
    centered = gd.getNextBoolean();
    magthresh = (float) gd.getNextNumber();
    rlength = (int) gd.getNextNumber();
    inc = (int) gd.getNextNumber();

    int width = imp.getWidth();
    int xregions = 1 + (int) (((float) width - (float) subsize) / (float) stepsize);
    int newwidth = xregions * subsize;
    int height = imp.getHeight();
    int yregions = 1 + (int) (((float) height - (float) subsize) / (float) stepsize);
    int newheight = yregions * subsize;
    ImageStack stack = imp.getStack();
    int slices = imp.getNSlices();
    int channels = imp.getNChannels();
    int frames = imp.getNFrames();
    if (frames == 1) {
      frames = slices;
      slices = 1;
    }

    Roi roi = imp.getRoi();
    if (roi == null) {
      roi = new Roi(0, 0, width, height);
    }

    STICS_map map = new STICS_map(subsize, stepsize);
    Object[] tseries = jutils.get3DTSeries(stack, 0, 0, frames, slices, channels);
    map.update_STICS_map(tseries, width, height, 0, rlength, roi.getPolygon(), shift);
    FloatProcessor fp =
        map.get_map(scaling, ftime, stepsize, centered, norm, multiplier, stepsize, magthresh);
    ImageStack vector_stack = new ImageStack(fp.getWidth(), fp.getHeight());
    vector_stack.addSlice("", fp);
    float[][] vel = map.get_scaled_velocities(scaling, ftime, stepsize);
    ImageStack velstack = new ImageStack(map.xregions, map.yregions);
    velstack.addSlice("", vel[0]);
    velstack.addSlice("", vel[1]);
    int velframes = 2;
    IJ.showStatus("frame " + 0 + " calculated");
    for (int i = inc; i < (frames - rlength); i += inc) {
      map.update_STICS_map(tseries, width, height, i, rlength, roi.getPolygon(), shift);
      FloatProcessor fp2 =
          map.get_map(scaling, ftime, stepsize, centered, norm, multiplier, stepsize, magthresh);
      vector_stack.addSlice("", fp2);
      vel = map.get_scaled_velocities(scaling, ftime, stepsize);
      velstack.addSlice("", vel[0]);
      velstack.addSlice("", vel[1]);
      velframes += 2;
      IJ.showStatus("frame " + i + " calculated");
    }
    (new ImagePlus("STICS Vectors", vector_stack)).show();
    ImagePlus imp3 = new ImagePlus("Velocities", velstack);
    imp3.setOpenAsHyperStack(true);
    imp3.setDimensions(2, 1, velframes / 2);
    new CompositeImage(imp3, CompositeImage.COLOR).show();
  }
Ejemplo n.º 15
0
    public BandAdjuster() {

      super("Threshold Colour");
      if (instance != null) {
        instance.toFront();
        return;
      }
      imp = WindowManager.getCurrentImage();
      if (imp == null) {
        IJ.beep();
        IJ.showStatus("No image");
        return;
      }
      IJ.run("Select None");
      thread = new Thread(this, "BandAdjuster");
      WindowManager.addWindow(this);
      instance = this;
      IJ.register(PasteController.class);

      ij = IJ.getInstance();
      Font font = new Font("SansSerif", Font.PLAIN, 10);
      GridBagLayout gridbag = new GridBagLayout();
      GridBagConstraints c = new GridBagConstraints();
      setLayout(gridbag);

      int y = 0;
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      labelh = new Label("Hue", Label.CENTER);
      add(labelh, c);

      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(7, 0, 0, 0);
      labelf = new Label("Filter type", Label.RIGHT);
      add(labelf, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(plot, c);

      // checkboxes
      panelh = new Panel();
      filterTypeH = new CheckboxGroup();
      bandPassH = new Checkbox("Pass");
      bandPassH.setCheckboxGroup(filterTypeH);
      bandPassH.addItemListener(this);
      panelh.add(bandPassH);
      bandStopH = new Checkbox("Stop");
      bandStopH.setCheckboxGroup(filterTypeH);
      bandStopH.addItemListener(this);
      panelh.add(bandStopH);
      bandPassH.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelh, c);

      // minHue slider
      minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);

      add(minSlider, c);
      minSlider.addAdjustmentListener(this);
      minSlider.setUnitIncrement(1);

      // minHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label1 = new Label("       ", Label.LEFT);
      label1.setFont(font);
      add(label1, c);

      // maxHue sliderHue
      maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider, c);
      maxSlider.addAdjustmentListener(this);
      maxSlider.setUnitIncrement(1);

      // maxHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label2 = new Label("       ", Label.LEFT);
      label2.setFont(font);
      add(label2, c);

      // =====
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labels = new Label("Saturation", Label.CENTER);
      add(labels, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(splot, c);

      // checkboxes
      panels = new Panel();
      filterTypeS = new CheckboxGroup();
      bandPassS = new Checkbox("Pass");
      bandPassS.setCheckboxGroup(filterTypeS);
      bandPassS.addItemListener(this);
      panels.add(bandPassS);
      bandStopS = new Checkbox("Stop");
      bandStopS.setCheckboxGroup(filterTypeS);
      bandStopS.addItemListener(this);
      panels.add(bandStopS);
      bandPassS.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panels, c);

      // minSat slider
      minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider2, c);
      minSlider2.addAdjustmentListener(this);
      minSlider2.setUnitIncrement(1);

      // minSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label3 = new Label("       ", Label.LEFT);
      label3.setFont(font);
      add(label3, c);

      // maxSat slider
      maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider2, c);
      maxSlider2.addAdjustmentListener(this);
      maxSlider2.setUnitIncrement(1);

      // maxSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label4 = new Label("       ", Label.LEFT);
      label4.setFont(font);
      add(label4, c);

      // =====
      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labelb = new Label("Brightness", Label.CENTER);
      add(labelb, c);

      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(bplot, c);

      // checkboxes
      panelb = new Panel();
      filterTypeB = new CheckboxGroup();
      bandPassB = new Checkbox("Pass");
      bandPassB.setCheckboxGroup(filterTypeB);
      bandPassB.addItemListener(this);
      panelb.add(bandPassB);
      bandStopB = new Checkbox("Stop");
      bandStopB.setCheckboxGroup(filterTypeB);
      bandStopB.addItemListener(this);
      panelb.add(bandStopB);
      bandPassB.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelb, c);

      // minBri slider
      minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider3, c);
      minSlider3.addAdjustmentListener(this);
      minSlider3.setUnitIncrement(1);

      // minBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label5 = new Label("       ", Label.LEFT);
      label5.setFont(font);
      add(label5, c);

      // maxBri slider
      maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider3, c);
      maxSlider3.addAdjustmentListener(this);
      maxSlider3.setUnitIncrement(1);

      // maxBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label6 = new Label("       ", Label.LEFT);
      label6.setFont(font);
      add(label6, c);

      // =====
      panelt = new Panel();
      threshold = new Checkbox("Threshold");
      threshold.addItemListener(this);
      panelt.add(threshold);

      invert = new Checkbox("Invert");
      invert.addItemListener(this);
      panelt.add(invert);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelt, c);

      // buttons
      panel = new Panel();
      // panel.setLayout(new GridLayout(2, 2, 0, 0));
      originalB = new Button("Original");
      originalB.setEnabled(false);
      originalB.addActionListener(this);
      originalB.addKeyListener(ij);
      panel.add(originalB);

      filteredB = new Button("Filtered");
      filteredB.setEnabled(false);
      filteredB.addActionListener(this);
      filteredB.addKeyListener(ij);
      panel.add(filteredB);

      stackB = new Button("Stack");
      stackB.addActionListener(this);
      stackB.addKeyListener(ij);
      panel.add(stackB);

      helpB = new Button("Help");
      helpB.addActionListener(this);
      helpB.addKeyListener(ij);
      panel.add(helpB);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panel, c);

      panelMode = new Panel();

      sampleB = new Button("Sample");
      sampleB.addActionListener(this);
      sampleB.addKeyListener(ij);
      panelMode.add(sampleB);

      colourMode = new CheckboxGroup();
      hsb = new Checkbox("HSB");
      hsb.setCheckboxGroup(colourMode);
      hsb.addItemListener(this);
      panelMode.add(hsb);
      hsb.setState(true);
      rgb = new Checkbox("RGB");
      rgb.setCheckboxGroup(colourMode);
      rgb.addItemListener(this);
      panelMode.add(rgb);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelMode, c);

      addKeyListener(ij); // ImageJ handles keyboard shortcuts
      pack();
      GUI.center(this);
      setVisible(true);

      ip = setup(imp);
      if (ip == null) {
        imp.unlock();
        IJ.beep();
        IJ.showStatus("RGB image cannot be thresholded");
        return;
      }
      thread.start();
    }
  public void run(ImageProcessor ip) {
    String[] imageNames = getOpenImageNames();
    if (imageNames[0] == "None") {
      IJ.error("need at least 2 binary open images");
      return;
    }
    double previousMinOverlap = Prefs.get("BVTB.BinaryFeatureExtractor.minOverlap", 0);
    boolean previousCombine = Prefs.get("BVTB.BinaryFeatureExtractor.combine", false);

    GenericDialog gd = new GenericDialog("Binary Feature Extractor");
    gd.addChoice("Objects image", imageNames, imageNames[0]);
    gd.addChoice("Selector image", imageNames, imageNames[1]);
    gd.addNumericField("Object_overlap in % (0=off)", previousMinOverlap, 0, 9, "");
    gd.addCheckbox("Combine objects and selectors", previousCombine);
    gd.addCheckbox("Count output", true);
    gd.addCheckbox("Analysis tables", false);
    gd.showDialog();
    if (gd.wasCanceled()) {
      return;
    }
    String objectsImgTitle = gd.getNextChoice();
    String selectorsImgTitle = gd.getNextChoice();
    double minOverlap = gd.getNextNumber();
    boolean combineImages = gd.getNextBoolean();
    boolean showCountOutput = gd.getNextBoolean();
    boolean showAnalysis = gd.getNextBoolean();
    if (gd.invalidNumber() || minOverlap < 0 || minOverlap > 100) {
      IJ.error("invalid number");
      return;
    }
    Prefs.set("BVTB.BinaryFeatureExtractor.minOverlap", minOverlap);
    Prefs.set("BVTB.BinaryFeatureExtractor.combine", combineImages);

    if (objectsImgTitle.equals(selectorsImgTitle)) {
      IJ.error("images need to be different");
      return;
    }

    ImagePlus objectsImp = WindowManager.getImage(objectsImgTitle);
    ImageProcessor objectsIP = objectsImp.getProcessor();
    ImagePlus selectorsImp = WindowManager.getImage(selectorsImgTitle);
    ImageProcessor selectorsIP = selectorsImp.getProcessor();

    if (!objectsIP.isBinary() || !selectorsIP.isBinary()) {
      IJ.error("works with 8-bit binary images only");
      return;
    }

    if ((objectsImp.getWidth() != selectorsImp.getWidth())
        || objectsImp.getHeight() != selectorsImp.getHeight()) {
      IJ.error("images need to be of the same size");
      return;
    }

    // close any existing RoiManager before instantiating a new one for this analysis
    RoiManager oldRM = RoiManager.getInstance2();
    if (oldRM != null) {
      oldRM.close();
    }

    RoiManager objectsRM = new RoiManager(true);
    ResultsTable objectsRT = new ResultsTable();
    ParticleAnalyzer analyzeObjects =
        new ParticleAnalyzer(analyzerOptions, measurementFlags, objectsRT, 0.0, 999999999.9);
    analyzeObjects.setRoiManager(objectsRM);

    analyzeObjects.analyze(objectsImp);
    objectsRM.runCommand("Show None");
    int objectNumber = objectsRT.getCounter();

    Roi[] objectRoi = objectsRM.getRoisAsArray();

    ResultsTable measureSelectorsRT = new ResultsTable();
    Analyzer overlapAnalyzer = new Analyzer(selectorsImp, measurementFlags, measureSelectorsRT);

    ImagePlus outputImp =
        IJ.createImage("output", "8-bit black", objectsImp.getWidth(), objectsImp.getHeight(), 1);
    ImageProcessor outputIP = outputImp.getProcessor();

    double[] measuredOverlap = new double[objectNumber];

    outputIP.setValue(255.0);
    for (int o = 0; o < objectNumber; o++) {
      selectorsImp.killRoi();
      selectorsImp.setRoi(objectRoi[o]);
      overlapAnalyzer.measure();
      measuredOverlap[o] = measureSelectorsRT.getValue("%Area", o);
      if (minOverlap != 0.0 && measuredOverlap[o] >= minOverlap) {
        outputIP.fill(objectRoi[o]);
        finalCount++;
      } else if (minOverlap == 0.0 && measuredOverlap[o] > 0.0) {
        outputIP.fill(objectRoi[o]);
        finalCount++;
      }
    }
    // measureSelectorsRT.show("Objects");

    selectorsImp.killRoi();
    RoiManager selectorRM = new RoiManager(true);
    ResultsTable selectorRT = new ResultsTable();
    ParticleAnalyzer.setRoiManager(selectorRM);
    ParticleAnalyzer analyzeSelectors =
        new ParticleAnalyzer(analyzerOptions, measurementFlags, selectorRT, 0.0, 999999999.9);
    analyzeSelectors.analyze(selectorsImp);
    selectorRM.runCommand("Show None");
    int selectorNumber = selectorRT.getCounter();

    if (combineImages) {
      outputImp.updateAndDraw();
      Roi[] selectorRoi = selectorRM.getRoisAsArray();

      ResultsTable measureObjectsRT = new ResultsTable();
      Analyzer selectorAnalyzer = new Analyzer(outputImp, measurementFlags, measureObjectsRT);

      double[] selectorOverlap = new double[selectorNumber];
      outputIP.setValue(255.0);
      for (int s = 0; s < selectorNumber; s++) {
        outputImp.killRoi();
        outputImp.setRoi(selectorRoi[s]);
        selectorAnalyzer.measure();
        selectorOverlap[s] = measureObjectsRT.getValue("%Area", s);
        if (selectorOverlap[s] > 0.0d) {
          outputIP.fill(selectorRoi[s]);
        }
      }
      selectorRoi = null;
      selectorAnalyzer = null;
      measureObjectsRT = null;
    }
    // selectorRT.show("Selectors");
    outputImp.killRoi();
    String outputImageTitle = WindowManager.getUniqueName("Extracted_" + objectsImgTitle);
    outputImp.setTitle(outputImageTitle);
    outputImp.show();
    outputImp.changes = true;

    if (showCountOutput) {
      String[] openTextWindows = WindowManager.getNonImageTitles();
      boolean makeNewTable = true;
      for (int w = 0; w < openTextWindows.length; w++) {
        if (openTextWindows[w].equals("BFE_Results")) {
          makeNewTable = false;
        }
      }

      TextWindow existingCountTable = ResultsTable.getResultsWindow();
      if (makeNewTable) {
        countTable = new ResultsTable();
        countTable.setPrecision(0);
        countTable.setValue("Image", 0, outputImageTitle);
        countTable.setValue("Objects", 0, objectNumber);
        countTable.setValue("Selectors", 0, selectorNumber);
        countTable.setValue("Extracted", 0, finalCount);
        countTable.show("BFE_Results");
      } else {
        IJ.renameResults("BFE_Results", "Results");
        countTable = ResultsTable.getResultsTable();
        countTable.setPrecision(0);
        countTable.incrementCounter();
        countTable.addValue("Image", outputImageTitle);
        countTable.addValue("Objects", objectNumber);
        countTable.addValue("Selectors", selectorNumber);
        countTable.addValue("Extracted", finalCount);
        IJ.renameResults("Results", "BFE_Results");
        countTable.show("BFE_Results");
      }
    }

    if (showAnalysis) {
      ResultsTable extractedRT = new ResultsTable();
      ParticleAnalyzer analyzeExtracted =
          new ParticleAnalyzer(
              ParticleAnalyzer.CLEAR_WORKSHEET | ParticleAnalyzer.RECORD_STARTS,
              measurementFlags,
              extractedRT,
              0.0,
              999999999.9);
      analyzeExtracted.analyze(outputImp);
      objectsRT.show("Objects");
      selectorRT.show("Selectors");
      extractedRT.show("Extracted");
    } else {
      objectsRT = null;
      selectorRT = null;
    }

    objectsRM = null;
    measureSelectorsRT = null;
    analyzeObjects = null;
    overlapAnalyzer = null;
    objectRoi = null;
    selectorRM = null;

    objectsImp.killRoi();
    objectsImp.changes = false;
    selectorsImp.changes = false;
  }
Ejemplo n.º 17
0
  boolean showDialog(ImageProcessor ip) {
    String macroOptions = Macro.getOptions();
    if (macroOptions != null) {
      if (macroOptions.indexOf(" interpolate") != -1)
        macroOptions.replaceAll(" interpolate", " interpolation=Bilinear");
      else if (macroOptions.indexOf(" interpolation=") == -1)
        macroOptions = macroOptions + " interpolation=None";
      Macro.setOptions(macroOptions);
    }
    int bitDepth = imp.getBitDepth();
    int stackSize = imp.getStackSize();
    boolean isStack = stackSize > 1;
    oldDepth = stackSize;
    if (isStack) {
      xstr = "1.0";
      ystr = "1.0";
      zstr = "1.0";
    }
    r = ip.getRoi();
    int width = newWidth;
    if (width == 0) width = r.width;
    int height = (int) ((double) width * r.height / r.width);
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    zscale = 1.0;
    if (xscale != 0.0 && yscale != 0.0) {
      width = (int) (r.width * xscale);
      height = (int) (r.height * yscale);
    } else {
      xstr = "-";
      ystr = "-";
    }
    GenericDialog gd = new GenericDialog("Scale");
    gd.addStringField("X Scale:", xstr);
    gd.addStringField("Y Scale:", ystr);
    if (isStack) gd.addStringField("Z Scale:", zstr);
    gd.setInsets(5, 0, 5);
    gd.addStringField("Width (pixels):", "" + width);
    gd.addStringField("Height (pixels):", "" + height);
    if (isStack) {
      String label = "Depth (images):";
      if (imp.isHyperStack()) {
        int slices = imp.getNSlices();
        int frames = imp.getNFrames();
        if (slices == 1 && frames > 1) {
          label = "Depth (frames):";
          oldDepth = frames;
        } else {
          label = "Depth (slices):";
          oldDepth = slices;
        }
      }
      gd.addStringField(label, "" + oldDepth);
    }
    fields = gd.getStringFields();
    for (int i = 0; i < fields.size(); i++) {
      ((TextField) fields.elementAt(i)).addTextListener(this);
      ((TextField) fields.elementAt(i)).addFocusListener(this);
    }
    xField = (TextField) fields.elementAt(0);
    yField = (TextField) fields.elementAt(1);
    if (isStack) {
      zField = (TextField) fields.elementAt(2);
      widthField = (TextField) fields.elementAt(3);
      heightField = (TextField) fields.elementAt(4);
      depthField = (TextField) fields.elementAt(5);
    } else {
      widthField = (TextField) fields.elementAt(2);
      heightField = (TextField) fields.elementAt(3);
    }
    fieldWithFocus = xField;
    gd.addChoice("Interpolation:", methods, methods[interpolationMethod]);
    if (bitDepth == 8 || bitDepth == 24)
      gd.addCheckbox("Fill with background color", fillWithBackground);
    gd.addCheckbox("Average when downsizing", averageWhenDownsizing);
    boolean hyperstack = imp.isHyperStack() || imp.isComposite();
    if (isStack && !hyperstack) gd.addCheckbox("Process entire stack", processStack);
    gd.addCheckbox("Create new window", newWindow);
    title = WindowManager.getUniqueName(imp.getTitle());
    gd.setInsets(10, 0, 0);
    gd.addStringField("Title:", title, 12);
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    xstr = gd.getNextString();
    ystr = gd.getNextString();
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    if (isStack) {
      zstr = gd.getNextString();
      zscale = Tools.parseDouble(ystr, 0.0);
    }
    String wstr = gd.getNextString();
    newWidth = (int) Tools.parseDouble(wstr, 0);
    newHeight = (int) Tools.parseDouble(gd.getNextString(), 0);
    if (newHeight != 0 && (wstr.equals("-") || wstr.equals("0")))
      newWidth = (int) (newHeight * (double) r.width / r.height);
    if (newWidth == 0 || newHeight == 0) {
      IJ.error("Scaler", "Width or height is 0");
      return false;
    }
    if (xscale > 0.0 && yscale > 0.0) {
      newWidth = (int) (r.width * xscale);
      newHeight = (int) (r.height * yscale);
    }
    if (isStack) newDepth = (int) Tools.parseDouble(gd.getNextString(), 0);
    interpolationMethod = gd.getNextChoiceIndex();
    if (bitDepth == 8 || bitDepth == 24) fillWithBackground = gd.getNextBoolean();
    averageWhenDownsizing = gd.getNextBoolean();
    if (isStack && !hyperstack) processStack = gd.getNextBoolean();
    if (hyperstack) processStack = true;
    newWindow = gd.getNextBoolean();
    if (xscale == 0.0) {
      xscale = (double) newWidth / r.width;
      yscale = (double) newHeight / r.height;
    }
    title = gd.getNextString();

    if (fillWithBackground) {
      Color bgc = Toolbar.getBackgroundColor();
      if (bitDepth == 8) bgValue = ip.getBestIndex(bgc);
      else if (bitDepth == 24) bgValue = bgc.getRGB();
    } else bgValue = 0.0;
    return true;
  }
Ejemplo n.º 18
0
  boolean showDialog(ImageProcessor ip) {
    int bitDepth = imp.getBitDepth();
    boolean isStack = imp.getStackSize() > 1;
    r = ip.getRoi();
    int width = newWidth;
    if (width == 0) width = r.width;
    int height = (int) ((double) width * r.height / r.width);
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    if (xscale != 0.0 && yscale != 0.0) {
      width = (int) (r.width * xscale);
      height = (int) (r.height * yscale);
    } else {
      xstr = "-";
      ystr = "-";
    }
    GenericDialog gd = new GenericDialog("Scale");
    gd.addStringField("X Scale (0.05-25):", xstr);
    gd.addStringField("Y Scale (0.05-25):", ystr);
    gd.setInsets(5, 0, 5);
    gd.addStringField("Width (pixels):", "" + width);
    gd.addStringField("Height (pixels):", "" + height);
    fields = gd.getStringFields();
    for (int i = 0; i < 3; i++) {
      ((TextField) fields.elementAt(i)).addTextListener(this);
      ((TextField) fields.elementAt(i)).addFocusListener(this);
    }
    xField = (TextField) fields.elementAt(0);
    yField = (TextField) fields.elementAt(1);
    widthField = (TextField) fields.elementAt(2);
    heightField = (TextField) fields.elementAt(3);
    fieldWithFocus = xField;
    gd.addCheckbox("Interpolate", interpolate);
    if (bitDepth == 8 || bitDepth == 24)
      gd.addCheckbox("Fill with Background Color", fillWithBackground);
    if (isStack) gd.addCheckbox("Process Entire Stack", processStack);
    gd.addCheckbox("Create New Window", newWindow);
    title = WindowManager.getUniqueName(imp.getTitle());
    gd.setInsets(10, 0, 0);
    gd.addStringField("Title:", title, 12);
    gd.showDialog();
    if (gd.wasCanceled()) return false;
    xstr = gd.getNextString();
    ystr = gd.getNextString();
    xscale = Tools.parseDouble(xstr, 0.0);
    yscale = Tools.parseDouble(ystr, 0.0);
    String wstr = gd.getNextString();
    newWidth = (int) Tools.parseDouble(wstr, 0);
    newHeight = (int) Tools.parseDouble(gd.getNextString(), 0);
    if (newHeight != 0 && (wstr.equals("-") || wstr.equals("0")))
      newWidth = (int) (newHeight * (double) r.width / r.height);
    if (newWidth == 0 || newHeight == 0) {
      IJ.error("Invalid width or height entered");
      return false;
    }
    if (xscale > 25.0) xscale = 25.0;
    if (yscale > 25.0) yscale = 25.0;
    if (xscale > 0.0 && yscale > 0.0) {
      newWidth = (int) (r.width * xscale);
      newHeight = (int) (r.height * yscale);
    }
    interpolate = gd.getNextBoolean();
    if (bitDepth == 8 || bitDepth == 24) fillWithBackground = gd.getNextBoolean();
    if (isStack) processStack = gd.getNextBoolean();
    newWindow = gd.getNextBoolean();
    if (!newWindow && xscale == 0.0) {
      xscale = (double) newWidth / r.width;
      yscale = (double) newHeight / r.height;
    }
    title = gd.getNextString();

    if (fillWithBackground) {
      Color bgc = Toolbar.getBackgroundColor();
      if (bitDepth == 8) bgValue = ip.getBestIndex(bgc);
      else if (bitDepth == 24) bgValue = bgc.getRGB();
    } else {
      if (bitDepth == 8) bgValue = ip.isInvertedLut() ? 0.0 : 255.0; // white
      else if (bitDepth == 24) bgValue = 0xffffffff; // white
    }
    return true;
  }