public PcrRegion next(String plate, int quadrantNumber, int numberOfRegions)
throws PlateFullException {
if (numberOfRegions == 0) {
throw new IllegalArgumentException("Dna blocks has no reps setup properly");
}
if (!iterator.hasNext()) {
throw new AllocationException("No more pcr wells available for allocation to the PcrRegion");
}
int numberOfWellsToAllocate = wellLocations.size() / numberOfRegions;
LinkedList<PlateWellLocation> pcrRegionWells = new LinkedList<PlateWellLocation>();
LinkedList<PlateWellLocation> dnaWells = new LinkedList<PlateWellLocation>();
for (int i = 0; i < numberOfWellsToAllocate; i++) {
pcrRegionWells.add(iterator.next());
}
for (int i = 0; i < numberOfWellsToAllocate; i++) {
dnaWells.add(dnaLocations.next());
}
return new PcrRegion(plate, currentRegionNumber++, quadrantNumber, pcrRegionWells, dnaWells);
}
public PcrRegionAllocator(List<PlateWellLocation> wellLocations) {
this.wellLocations = wellLocations;
iterator = wellLocations.iterator();
dnaLocations = PlateLocations.instance(UserPreferences.newInstance().getInt("dnaSource.type"));
}