public int getNumberOfSamplesForEvaluation() {
if (sampleNamesForEvaluation != null && !sampleNamesForEvaluation.isEmpty())
return sampleNamesForEvaluation.size();
else {
return numSamplesFromArgument;
}
}
/** Initialize the stratifications, evaluations, evaluation contexts, and reporting object */
public void initialize() {
// Just list the modules, and exit quickly.
if (LIST) {
variantEvalUtils.listModulesAndExit();
}
// maintain the full list of comps
comps.addAll(compsProvided);
if (dbsnp.dbsnp.isBound()) {
comps.add(dbsnp.dbsnp);
knowns.add(dbsnp.dbsnp);
}
// Add a dummy comp track if none exists
if (comps.size() == 0)
comps.add(
new RodBinding<VariantContext>(VariantContext.class, "none", "UNBOUND", "", new Tags()));
// Set up set of additional knowns
for (RodBinding<VariantContext> compRod : comps) {
if (KNOWN_NAMES.contains(compRod.getName())) knowns.add(compRod);
}
// Now that we have all the rods categorized, determine the sample list from the eval rods.
Map<String, VCFHeader> vcfRods = GATKVCFUtils.getVCFHeadersFromRods(getToolkit(), evals);
Set<String> vcfSamples =
SampleUtils.getSampleList(
vcfRods, GATKVariantContextUtils.GenotypeMergeType.REQUIRE_UNIQUE);
// Load the sample list, using an intermediate tree set to sort the samples
final Set<String> allSampleNames = SampleUtils.getSamplesFromCommandLineInput(vcfSamples);
sampleNamesForEvaluation.addAll(
new TreeSet<String>(
SampleUtils.getSamplesFromCommandLineInput(vcfSamples, SAMPLE_EXPRESSIONS)));
isSubsettingSamples = !sampleNamesForEvaluation.containsAll(allSampleNames);
if (Arrays.asList(STRATIFICATIONS_TO_USE).contains("Sample")) {
sampleNamesForStratification.addAll(sampleNamesForEvaluation);
}
sampleNamesForStratification.add(ALL_SAMPLE_NAME);
// Initialize select expressions
for (VariantContextUtils.JexlVCMatchExp jexl :
VariantContextUtils.initializeMatchExps(SELECT_NAMES, SELECT_EXPS)) {
SortableJexlVCMatchExp sjexl = new SortableJexlVCMatchExp(jexl.name, jexl.exp);
jexlExpressions.add(sjexl);
}
// Initialize the set of stratifications and evaluations to use
// The list of stratifiers and evaluators to use
final List<VariantStratifier> stratificationObjects =
variantEvalUtils.initializeStratificationObjects(
NO_STANDARD_STRATIFICATIONS, STRATIFICATIONS_TO_USE);
final Set<Class<? extends VariantEvaluator>> evaluationClasses =
variantEvalUtils.initializeEvaluationObjects(NO_STANDARD_MODULES, MODULES_TO_USE);
checkForIncompatibleEvaluatorsAndStratifiers(stratificationObjects, evaluationClasses);
for (VariantStratifier vs : stratificationObjects) {
if (vs.getName().equals("Filter")) byFilterIsEnabled = true;
else if (vs.getName().equals("Sample")) perSampleIsEnabled = true;
}
if (intervalsFile != null) {
boolean fail = true;
for (final VariantStratifier vs : stratificationObjects) {
if (vs.getClass().equals(IntervalStratification.class)) fail = false;
}
if (fail)
throw new UserException.BadArgumentValue(
"ST", "stratIntervals argument provided but -ST IntervalStratification not provided");
}
// Initialize the evaluation contexts
createStratificationStates(stratificationObjects, evaluationClasses);
// Load ancestral alignments
if (ancestralAlignmentsFile != null) {
try {
ancestralAlignments = new IndexedFastaSequenceFile(ancestralAlignmentsFile);
} catch (FileNotFoundException e) {
throw new ReviewedStingException(
String.format(
"The ancestral alignments file, '%s', could not be found",
ancestralAlignmentsFile.getAbsolutePath()));
}
}
// initialize CNVs
if (knownCNVsFile != null) {
knownCNVsByContig = createIntervalTreeByContig(knownCNVsFile);
}
}