Beispiel #1
0
 public int getNumberOfSamplesForEvaluation() {
   if (sampleNamesForEvaluation != null && !sampleNamesForEvaluation.isEmpty())
     return sampleNamesForEvaluation.size();
   else {
     return numSamplesFromArgument;
   }
 }
Beispiel #2
0
  /** Initialize the stratifications, evaluations, evaluation contexts, and reporting object */
  public void initialize() {
    // Just list the modules, and exit quickly.
    if (LIST) {
      variantEvalUtils.listModulesAndExit();
    }

    // maintain the full list of comps
    comps.addAll(compsProvided);
    if (dbsnp.dbsnp.isBound()) {
      comps.add(dbsnp.dbsnp);
      knowns.add(dbsnp.dbsnp);
    }

    // Add a dummy comp track if none exists
    if (comps.size() == 0)
      comps.add(
          new RodBinding<VariantContext>(VariantContext.class, "none", "UNBOUND", "", new Tags()));

    // Set up set of additional knowns
    for (RodBinding<VariantContext> compRod : comps) {
      if (KNOWN_NAMES.contains(compRod.getName())) knowns.add(compRod);
    }

    // Now that we have all the rods categorized, determine the sample list from the eval rods.
    Map<String, VCFHeader> vcfRods = GATKVCFUtils.getVCFHeadersFromRods(getToolkit(), evals);
    Set<String> vcfSamples =
        SampleUtils.getSampleList(
            vcfRods, GATKVariantContextUtils.GenotypeMergeType.REQUIRE_UNIQUE);

    // Load the sample list, using an intermediate tree set to sort the samples
    final Set<String> allSampleNames = SampleUtils.getSamplesFromCommandLineInput(vcfSamples);
    sampleNamesForEvaluation.addAll(
        new TreeSet<String>(
            SampleUtils.getSamplesFromCommandLineInput(vcfSamples, SAMPLE_EXPRESSIONS)));
    isSubsettingSamples = !sampleNamesForEvaluation.containsAll(allSampleNames);

    if (Arrays.asList(STRATIFICATIONS_TO_USE).contains("Sample")) {
      sampleNamesForStratification.addAll(sampleNamesForEvaluation);
    }
    sampleNamesForStratification.add(ALL_SAMPLE_NAME);

    // Initialize select expressions
    for (VariantContextUtils.JexlVCMatchExp jexl :
        VariantContextUtils.initializeMatchExps(SELECT_NAMES, SELECT_EXPS)) {
      SortableJexlVCMatchExp sjexl = new SortableJexlVCMatchExp(jexl.name, jexl.exp);
      jexlExpressions.add(sjexl);
    }

    // Initialize the set of stratifications and evaluations to use
    // The list of stratifiers and evaluators to use
    final List<VariantStratifier> stratificationObjects =
        variantEvalUtils.initializeStratificationObjects(
            NO_STANDARD_STRATIFICATIONS, STRATIFICATIONS_TO_USE);
    final Set<Class<? extends VariantEvaluator>> evaluationClasses =
        variantEvalUtils.initializeEvaluationObjects(NO_STANDARD_MODULES, MODULES_TO_USE);

    checkForIncompatibleEvaluatorsAndStratifiers(stratificationObjects, evaluationClasses);

    for (VariantStratifier vs : stratificationObjects) {
      if (vs.getName().equals("Filter")) byFilterIsEnabled = true;
      else if (vs.getName().equals("Sample")) perSampleIsEnabled = true;
    }

    if (intervalsFile != null) {
      boolean fail = true;
      for (final VariantStratifier vs : stratificationObjects) {
        if (vs.getClass().equals(IntervalStratification.class)) fail = false;
      }
      if (fail)
        throw new UserException.BadArgumentValue(
            "ST", "stratIntervals argument provided but -ST IntervalStratification not provided");
    }

    // Initialize the evaluation contexts
    createStratificationStates(stratificationObjects, evaluationClasses);

    // Load ancestral alignments
    if (ancestralAlignmentsFile != null) {
      try {
        ancestralAlignments = new IndexedFastaSequenceFile(ancestralAlignmentsFile);
      } catch (FileNotFoundException e) {
        throw new ReviewedStingException(
            String.format(
                "The ancestral alignments file, '%s', could not be found",
                ancestralAlignmentsFile.getAbsolutePath()));
      }
    }

    // initialize CNVs
    if (knownCNVsFile != null) {
      knownCNVsByContig = createIntervalTreeByContig(knownCNVsFile);
    }
  }