void updateLabels() {
   label1.setText("" + ((int) minHue));
   label2.setText("" + ((int) maxHue));
   label3.setText("" + ((int) minSat));
   label4.setText("" + ((int) maxSat));
   label5.setText("" + ((int) minBri));
   label6.setText("" + ((int) maxBri));
 }
 void updateNames() {
   if (isRGB) {
     labelh.setText("Red");
     labels.setText("Green");
     labelb.setText("Blue");
   } else {
     labelh.setText("Hue");
     labels.setText("Saturation");
     labelb.setText("Brightness");
   }
 }
 private void undoglobalfit() {
   for (int i = 0; i < ncurves; i++) {
     for (int j = 0; j < nparams; j++) {
       globalparams[i][j] = undoparams[i][j];
       globalformulas[i][j] = undoformulas[i][j];
       globalvflmatrix[i][j] = undovflmatrix[i][j];
     }
     for (int j = 0; j < xpts; j++) {
       for (int k = 0; k < ypts; k++) {
         fit[i][j][k] = undofit[i][j][k];
       }
     }
     if (i == dispcurve) {
       pwfit.updateSeries(fit[dispcurve], 1, true);
     }
     c2[i] = undoc2[i];
     c2array[i].setText("" + (float) c2[i]);
   }
   globalc2 = undoglobalc2;
   globalc2label.setText("Global chi^2 = " + (float) globalc2);
 }
  private void fitglobal() {
    int nparams = 11;
    int nsel = 0;
    for (int i = 0; i < ncurves; i++) {
      if (include[i]) {
        nsel++;
      }
    }
    double[][] params = new double[nsel][nparams];
    String[][] tempformulas = new String[nsel][nparams];
    double[][][] constraints = new double[2][nsel][nparams];
    int[][] vflmatrix = new int[nsel][nparams];

    int counter = 0;
    for (int i = 0; i < ncurves; i++) {
      if (include[i]) {
        for (int j = 0; j < nparams; j++) {
          params[counter][j] = globalparams[i][j];
          tempformulas[counter][j] = globalformulas[i][j];
          constraints[0][counter][j] = globalconstraints[0][i][j];
          constraints[1][counter][j] = globalconstraints[1][i][j];
          vflmatrix[counter][j] = globalvflmatrix[i][j];
        }
        counter++;
      }
      for (int j = 0; j < nparams; j++) {
        undoparams[i][j] = globalparams[i][j];
        undoformulas[i][j] = globalformulas[i][j];
        undovflmatrix[i][j] = globalvflmatrix[i][j];
      }
      for (int j = 0; j < xpts; j++) {
        for (int k = 0; k < ypts; k++) {
          undofit[i][j][k] = fit[i][j][k];
        }
      }
      undoc2[i] = c2[i];
    }
    undoglobalc2 = globalc2;
    if (showglobalfitdialog(params, tempformulas, vflmatrix)) {
      counter = 0;
      for (int i = 0; i < ncurves; i++) {
        if (include[i]) {
          for (int j = 0; j < nparams; j++) {
            globalparams[i][j] = params[counter][j];
            globalformulas[i][j] = tempformulas[counter][j];
            globalvflmatrix[i][j] = vflmatrix[counter][j];
          }
          counter++;
        }
      }
      double[] stats = new double[2];
      float[][] tempdata = new float[nsel][xpts * ypts];
      float[][] tempweights = new float[nsel][xpts * ypts];
      counter = 0;
      for (int i = 0; i < ncurves; i++) {
        if (include[i]) {
          for (int j = 0; j < xpts; j++) {
            for (int k = 0; k < ypts; k++) {
              tempdata[counter][j + k * xpts] = (float) ((double) pch[i][j][k] / (double) nmeas[i]);
              tempweights[counter][j + k * xpts] = weights[i][j][k];
            }
          }
          counter++;
        }
      }
      int tempmaxiter = globalfitclass.maxiter;
      if (checkc2) {
        globalfitclass.changemaxiter(0);
      }
      double[] tempc2vals = new double[nsel];
      IJ.showStatus("Fitting Globally");
      float[][] tempfit =
          globalfitclass.fitdata(
              params,
              vflmatrix,
              tempformulas,
              paramsnames,
              constraints,
              tempdata,
              tempweights,
              stats,
              tempc2vals,
              false);
      IJ.showStatus("Fit Complete");
      globalfitclass.changemaxiter(tempmaxiter);
      globalc2 = stats[1];
      globalc2label.setText("Global chi^2 = " + (float) globalc2);
      counter = 0;
      for (int i = 0; i < ncurves; i++) {
        if (include[i]) {
          for (int j = 0; j < xpts; j++) {
            for (int k = 0; k < ypts; k++) {
              fit[i][j][k] = tempfit[counter][j + xpts * k] * (float) nmeas[i];
            }
          }
          if (i == dispcurve) {
            pwfit.updateSeries(fit[dispcurve], 1, true);
          }
          for (int j = 0; j < nparams; j++) {
            globalparams[i][j] = params[counter][j];
          }
          c2[i] = tempc2vals[counter];
          c2array[i].setText("" + (float) c2[i]);
          counter++;
        }
      }
      float[] temp = pwfit.getLimits();
      temp[4] = 1.0f;
      pwfit.setLimits(temp);
    }
  }
  void init(String[] names1, float[][][] pch1, int psfflag1) {
    setLayout(null);
    names = names1;
    pch = pch1;
    psfflag = psfflag1;
    ncurves = pch.length;
    nparams = 11;
    xpts = pch[0].length;
    ypts = pch[0][0].length;

    checkarray = new Checkbox[ncurves];
    include = new boolean[ncurves];
    namearray = new TextField[ncurves + 1];
    int1array = new TextField[ncurves + 1];
    intensity1 = new double[ncurves + 1];
    e1array = new TextField[ncurves + 1];
    n1array = new TextField[ncurves + 1];
    bright1 = new double[ncurves + 1];
    number1 = new double[ncurves + 1];
    int2array = new TextField[ncurves + 1];
    intensity2 = new double[ncurves + 1];
    e2array = new TextField[ncurves + 1];
    n2array = new TextField[ncurves + 1];
    bright2 = new double[ncurves + 1];
    number2 = new double[ncurves + 1];
    eccarray = new TextField[ncurves + 1];
    brightcc = new double[ncurves + 1];
    eminccarray = new TextField[ncurves + 1];
    brightmincc = new double[ncurves + 1];
    c2array = new TextField[ncurves + 1];
    c2 = new double[ncurves + 1];
    nmeas = new int[ncurves + 1];
    avg = new float[xpts][ypts];
    indices = new int[ncurves];
    beta = 0.05;

    getintbright();
    for (int i = 0; i < ncurves; i++) {
      include[i] = true;
      indices[i] = i;
    }
    updateavg();

    int starty = 60;
    int startx = 10;
    int yinc = 25;
    for (int i = 0; i <= ncurves; i++) {
      if (i != ncurves) {
        checkarray[i] = new Checkbox("", include[i]);
        checkarray[i].setBounds(startx, starty + i * yinc, 20, 20);
        checkarray[i].addItemListener(this);
        add(checkarray[i]);
      }

      namearray[i] = new TextField(names[i]);
      namearray[i].setBounds(startx + 30, starty + i * yinc, 200, 20);
      add(namearray[i]);

      int1array[i] = new TextField("" + (float) intensity1[i]);
      int1array[i].setBounds(startx + 30 + 210, starty + i * yinc, 40, 20);
      add(int1array[i]);

      e1array[i] = new TextField("" + (float) bright1[i]);
      e1array[i].setBounds(startx + 30 + 210 + 50, starty + i * yinc, 40, 20);
      add(e1array[i]);

      n1array[i] = new TextField("" + (float) number1[i]);
      n1array[i].setBounds(startx + 30 + 210 + 50 + 50, starty + i * yinc, 40, 20);
      add(n1array[i]);

      int2array[i] = new TextField("" + (float) intensity2[i]);
      int2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(int2array[i]);

      e2array[i] = new TextField("" + (float) bright2[i]);
      e2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(e2array[i]);

      n2array[i] = new TextField("" + (float) number2[i]);
      n2array[i].setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(n2array[i]);

      eccarray[i] = new TextField("" + (float) brightcc[i]);
      eccarray[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(eccarray[i]);

      eminccarray[i] = new TextField("" + (float) brightmincc[i]);
      eminccarray[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 40, 20);
      add(eminccarray[i]);

      c2[i] = 0.0;
      c2array[i] = new TextField("" + (float) c2[i]);
      c2array[i].setBounds(
          startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty + i * yinc, 80, 20);
      add(c2array[i]);
    }

    namelabel = new Label("Filename");
    namelabel.setBounds(startx + 30, starty - 25, 100, 20);
    add(namelabel);

    intlabel = new Label("<Ig>");
    intlabel.setBounds(startx + 30 + 210, starty - 25, 40, 20);
    add(intlabel);

    brightlabel = new Label("<eg>");
    brightlabel.setBounds(startx + 30 + 210 + 50, starty - 25, 40, 20);
    add(brightlabel);

    nlabel = new Label("<Ng>");
    nlabel.setBounds(startx + 30 + 210 + 50 + 50, starty - 25, 40, 20);
    add(nlabel);

    int2label = new Label("<Ir>");
    int2label.setBounds(startx + 30 + 210 + 50 + 50 + 50, starty - 25, 40, 20);
    add(int2label);

    bright2label = new Label("<er>");
    bright2label.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(bright2label);

    n2label = new Label("<Nr>");
    n2label.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(n2label);

    brightcclabel = new Label("<ecc>");
    brightcclabel.setBounds(startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(brightcclabel);

    brightccminlabel = new Label("min");
    brightccminlabel.setBounds(
        startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 40, 20);
    add(brightccminlabel);

    c2label = new Label("chi^2");
    c2label.setBounds(
        startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50, starty - 25, 80, 20);
    add(c2label);

    int buttonsx = startx + 30 + 210 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 50 + 90;

    fitavgbutton = new Button("Fit Avg");
    fitavgbutton.setBounds(buttonsx, starty - 25, 100, 40);
    fitavgbutton.addActionListener(this);
    add(fitavgbutton);

    fitglobalbutton = new Button("Fit Global");
    fitglobalbutton.setBounds(buttonsx, starty - 25 + 50, 100, 40);
    fitglobalbutton.addActionListener(this);
    add(fitglobalbutton);

    clearparamsbutton = new Button("Reset Fit Params");
    clearparamsbutton.setBounds(buttonsx, starty - 25 + 50 + 50, 100, 40);
    clearparamsbutton.addActionListener(this);
    add(clearparamsbutton);

    checkc2 = false;

    fitclass = new NLLSfit(this, 0.0001, 50, 0.1);
    globalfitclass = new NLLSglobalfit(this, 0.0001, 50, 0.1);
    pchfunc = new pch2D((int) ((double) xpts * 1.5), (int) ((double) ypts * 1.5), psfflag);
    avgfit = new float[xpts][ypts];
    fit = new float[ncurves][xpts][ypts];

    xvals = new float[ncurves][xpts][ypts];
    for (int i = 0; i < ncurves; i++) {
      for (int j = 0; j < xpts; j++) {
        for (int k = 0; k < ypts; k++) {
          xvals[i][j][k] = (float) k;
          fit[i][j][k] = 1.0f;
        }
      }
    }

    globalc2label = new Label("Global chi^2 = " + (float) 0.0);
    globalc2label.setBounds(buttonsx, starty - 25 + 50 + 50 + 50, 140, 20);
    add(globalc2label);

    dispcurvelabel = new Label("Display Fit #");
    dispcurvelabel.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30, 70, 20);
    add(dispcurvelabel);

    dispcurvechoice = new Choice();
    for (int i = 0; i < ncurves; i++) {
      dispcurvechoice.add("" + (i + 1));
    }
    dispcurve = 0;
    dispcurvechoice.select(0);
    dispcurvechoice.setBounds(buttonsx + 80, starty - 25 + 50 + 50 + 50 + 30, 40, 20);
    dispcurvechoice.addItemListener(this);
    add(dispcurvechoice);

    betalabel = new Label("Bleedthrough f");
    betalabel.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30, 100, 20);
    add(betalabel);
    betaval = new TextField("" + (float) beta);
    betaval.setBounds(buttonsx + 110, starty - 25 + 50 + 50 + 50 + 30 + 30, 40, 20);
    betaval.addActionListener(this);
    add(betaval);

    beta = Double.parseDouble(betaval.getText());
    updatebeta();

    undobutton = new Button("Undo Global Fit");
    undobutton.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30 + 50, 100, 40);
    undobutton.addActionListener(this);
    add(undobutton);

    geterrorsbutton = new Button("Get Errors");
    geterrorsbutton.setBounds(buttonsx, starty - 25 + 50 + 50 + 50 + 30 + 30 + 50 + 50, 100, 40);
    geterrorsbutton.addActionListener(this);
    add(geterrorsbutton);

    copylabel = new Label("copyright 2009 Jay Unruh ([email protected]) non-profit use only");
    copylabel.setBounds(10, 790, 400, 20);
    add(copylabel);

    n_b_label = new Label("N and B Analysis");
    n_b_label.setBounds(250, 10, 100, 20);
    add(n_b_label);

    pwavg = new PlotWindow3D("Avg", "kg", "kr", "Frequency", avg, 0);
    pwavg.setLogAxes(false, false, true);
    pwavg.draw();
    pwavg.addPoints(avgfit, true, 0);
    float[] temp = pwavg.getLimits();
    temp[4] = 1.0f;
    pwavg.setLimits(temp);

    float[][] temppch = new float[xpts][ypts];
    for (int i = 0; i < xpts; i++) {
      System.arraycopy(pch[dispcurve][i], 0, temppch[i], 0, ypts);
    }
    pwfit = new PlotWindow3D("Selected Curve", "kg", "kr", "Frequency", temppch, 0);
    pwfit.setLogAxes(false, false, true);
    pwfit.draw();
    pwfit.addPoints(fit[dispcurve], true, 0);
    float[] temp2 = pwfit.getLimits();
    temp2[4] = 1.0f;
    pwfit.setLimits(temp2);

    resetparams();
    repaint();
  }
Beispiel #6
0
 void showStatus(String s) {
   statusLine.setText(s);
 }
Beispiel #7
0
  /**
   * If 'applet' is not null, creates a new ImageJ frame that runs as an applet. If 'mode' is
   * ImageJ.EMBEDDED and 'applet is null, creates an embedded (non-standalone) version of ImageJ.
   */
  public ImageJ(java.applet.Applet applet, int mode) {
    super("ImageJ");
    embedded = applet == null && (mode == EMBEDDED || mode == NO_SHOW);
    this.applet = applet;
    String err1 = Prefs.load(this, applet);
    if (IJ.isLinux()) {
      backgroundColor = new Color(240, 240, 240);
      setBackground(backgroundColor);
    }
    Menus m = new Menus(this, applet);
    String err2 = m.addMenuBar();
    m.installPopupMenu(this);
    setLayout(new GridLayout(2, 1));

    // Tool bar
    toolbar = new Toolbar();
    toolbar.addKeyListener(this);
    add(toolbar);

    // Status bar
    statusBar = new Panel();
    statusBar.setLayout(new BorderLayout());
    statusBar.setForeground(Color.black);
    statusBar.setBackground(backgroundColor);
    statusLine = new Label();
    statusLine.setFont(SansSerif12);
    statusLine.addKeyListener(this);
    statusLine.addMouseListener(this);
    statusBar.add("Center", statusLine);
    progressBar = new ProgressBar(120, 20);
    progressBar.addKeyListener(this);
    progressBar.addMouseListener(this);
    statusBar.add("East", progressBar);
    statusBar.setSize(toolbar.getPreferredSize());
    add(statusBar);

    IJ.init(this, applet);
    addKeyListener(this);
    addWindowListener(this);
    setFocusTraversalKeysEnabled(false);

    Point loc = getPreferredLocation();
    Dimension tbSize = toolbar.getPreferredSize();
    int ijWidth = tbSize.width + 10;
    int ijHeight = 100;
    setCursor(Cursor.getDefaultCursor()); // work-around for JDK 1.1.8 bug
    if (mode != NO_SHOW) {
      if (IJ.isWindows())
        try {
          setIcon();
        } catch (Exception e) {
        }
      setBounds(loc.x, loc.y, ijWidth, ijHeight); // needed for pack to work
      setLocation(loc.x, loc.y);
      pack();
      setResizable(!(IJ.isMacintosh() || IJ.isWindows())); // make resizable on Linux
      show();
    }
    if (err1 != null) IJ.error(err1);
    if (err2 != null) {
      IJ.error(err2);
      IJ.runPlugIn("ij.plugin.ClassChecker", "");
    }
    m.installStartupMacroSet();
    if (IJ.isMacintosh() && applet == null) {
      Object qh = null;
      qh = IJ.runPlugIn("MacAdapter", "");
      if (qh == null) IJ.runPlugIn("QuitHandler", "");
    }
    if (applet == null) IJ.runPlugIn("ij.plugin.DragAndDrop", "");
    String str = m.getMacroCount() == 1 ? " macro" : " macros";
    IJ.showStatus(version() + m.getPluginCount() + " commands; " + m.getMacroCount() + str);
    // if (applet==null && !embedded && Prefs.runSocketListener)
    //	new SocketListener();
    configureProxy();
    if (applet == null) loadCursors();
  }
    public BandAdjuster() {

      super("Threshold Colour");
      if (instance != null) {
        instance.toFront();
        return;
      }
      imp = WindowManager.getCurrentImage();
      if (imp == null) {
        IJ.beep();
        IJ.showStatus("No image");
        return;
      }
      IJ.run("Select None");
      thread = new Thread(this, "BandAdjuster");
      WindowManager.addWindow(this);
      instance = this;
      IJ.register(PasteController.class);

      ij = IJ.getInstance();
      Font font = new Font("SansSerif", Font.PLAIN, 10);
      GridBagLayout gridbag = new GridBagLayout();
      GridBagConstraints c = new GridBagConstraints();
      setLayout(gridbag);

      int y = 0;
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      labelh = new Label("Hue", Label.CENTER);
      add(labelh, c);

      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(7, 0, 0, 0);
      labelf = new Label("Filter type", Label.RIGHT);
      add(labelf, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(plot, c);

      // checkboxes
      panelh = new Panel();
      filterTypeH = new CheckboxGroup();
      bandPassH = new Checkbox("Pass");
      bandPassH.setCheckboxGroup(filterTypeH);
      bandPassH.addItemListener(this);
      panelh.add(bandPassH);
      bandStopH = new Checkbox("Stop");
      bandStopH.setCheckboxGroup(filterTypeH);
      bandStopH.addItemListener(this);
      panelh.add(bandStopH);
      bandPassH.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelh, c);

      // minHue slider
      minSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);

      add(minSlider, c);
      minSlider.addAdjustmentListener(this);
      minSlider.setUnitIncrement(1);

      // minHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label1 = new Label("       ", Label.LEFT);
      label1.setFont(font);
      add(label1, c);

      // maxHue sliderHue
      maxSlider = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider, c);
      maxSlider.addAdjustmentListener(this);
      maxSlider.setUnitIncrement(1);

      // maxHue slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label2 = new Label("       ", Label.LEFT);
      label2.setFont(font);
      add(label2, c);

      // =====
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labels = new Label("Saturation", Label.CENTER);
      add(labels, c);

      // plot
      c.gridx = 0;
      c.gridy = y;
      c.gridwidth = 1;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(splot, c);

      // checkboxes
      panels = new Panel();
      filterTypeS = new CheckboxGroup();
      bandPassS = new Checkbox("Pass");
      bandPassS.setCheckboxGroup(filterTypeS);
      bandPassS.addItemListener(this);
      panels.add(bandPassS);
      bandStopS = new Checkbox("Stop");
      bandStopS.setCheckboxGroup(filterTypeS);
      bandStopS.addItemListener(this);
      panels.add(bandStopS);
      bandPassS.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panels, c);

      // minSat slider
      minSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider2, c);
      minSlider2.addAdjustmentListener(this);
      minSlider2.setUnitIncrement(1);

      // minSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label3 = new Label("       ", Label.LEFT);
      label3.setFont(font);
      add(label3, c);

      // maxSat slider
      maxSlider2 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider2, c);
      maxSlider2.addAdjustmentListener(this);
      maxSlider2.setUnitIncrement(1);

      // maxSat slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label4 = new Label("       ", Label.LEFT);
      label4.setFont(font);
      add(label4, c);

      // =====
      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y++;
      c.weightx = 0;
      c.insets = new Insets(10, 0, 0, 0);
      labelb = new Label("Brightness", Label.CENTER);
      add(labelb, c);

      c.gridx = 0;
      c.gridwidth = 1;
      c.gridy = y;
      c.fill = c.BOTH;
      c.anchor = c.CENTER;
      c.insets = new Insets(0, 5, 0, 0);
      add(bplot, c);

      // checkboxes
      panelb = new Panel();
      filterTypeB = new CheckboxGroup();
      bandPassB = new Checkbox("Pass");
      bandPassB.setCheckboxGroup(filterTypeB);
      bandPassB.addItemListener(this);
      panelb.add(bandPassB);
      bandStopB = new Checkbox("Stop");
      bandStopB.setCheckboxGroup(filterTypeB);
      bandStopB.addItemListener(this);
      panelb.add(bandStopB);
      bandPassB.setState(true);
      c.gridx = 1;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(5, 0, 0, 0);
      add(panelb, c);

      // minBri slider
      minSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 90 : 100;
      c.fill = c.HORIZONTAL;
      c.insets = new Insets(5, 5, 0, 0);
      add(minSlider3, c);
      minSlider3.addAdjustmentListener(this);
      minSlider3.setUnitIncrement(1);

      // minBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = IJ.isMacintosh() ? 10 : 0;
      c.insets = new Insets(5, 0, 0, 0);
      label5 = new Label("       ", Label.LEFT);
      label5.setFont(font);
      add(label5, c);

      // maxBri slider
      maxSlider3 = new Scrollbar(Scrollbar.HORIZONTAL, 0, 1, 0, sliderRange);
      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 1;
      c.weightx = 100;
      c.insets = new Insets(5, 5, 0, 0);
      add(maxSlider3, c);
      maxSlider3.addAdjustmentListener(this);
      maxSlider3.setUnitIncrement(1);

      // maxBri slider label
      c.gridx = 1;
      c.gridwidth = 1;
      c.weightx = 0;
      c.insets = new Insets(5, 0, 0, 0);
      label6 = new Label("       ", Label.LEFT);
      label6.setFont(font);
      add(label6, c);

      // =====
      panelt = new Panel();
      threshold = new Checkbox("Threshold");
      threshold.addItemListener(this);
      panelt.add(threshold);

      invert = new Checkbox("Invert");
      invert.addItemListener(this);
      panelt.add(invert);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelt, c);

      // buttons
      panel = new Panel();
      // panel.setLayout(new GridLayout(2, 2, 0, 0));
      originalB = new Button("Original");
      originalB.setEnabled(false);
      originalB.addActionListener(this);
      originalB.addKeyListener(ij);
      panel.add(originalB);

      filteredB = new Button("Filtered");
      filteredB.setEnabled(false);
      filteredB.addActionListener(this);
      filteredB.addKeyListener(ij);
      panel.add(filteredB);

      stackB = new Button("Stack");
      stackB.addActionListener(this);
      stackB.addKeyListener(ij);
      panel.add(stackB);

      helpB = new Button("Help");
      helpB.addActionListener(this);
      helpB.addKeyListener(ij);
      panel.add(helpB);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panel, c);

      panelMode = new Panel();

      sampleB = new Button("Sample");
      sampleB.addActionListener(this);
      sampleB.addKeyListener(ij);
      panelMode.add(sampleB);

      colourMode = new CheckboxGroup();
      hsb = new Checkbox("HSB");
      hsb.setCheckboxGroup(colourMode);
      hsb.addItemListener(this);
      panelMode.add(hsb);
      hsb.setState(true);
      rgb = new Checkbox("RGB");
      rgb.setCheckboxGroup(colourMode);
      rgb.addItemListener(this);
      panelMode.add(rgb);

      c.gridx = 0;
      c.gridy = y++;
      c.gridwidth = 2;
      c.insets = new Insets(0, 0, 0, 0);
      add(panelMode, c);

      addKeyListener(ij); // ImageJ handles keyboard shortcuts
      pack();
      GUI.center(this);
      setVisible(true);

      ip = setup(imp);
      if (ip == null) {
        imp.unlock();
        IJ.beep();
        IJ.showStatus("RGB image cannot be thresholded");
        return;
      }
      thread.start();
    }