/*------------------------------------------------------------------*/
  void setupProgressBar() {
    int height = imp.getHeight();
    int width = imp.getWidth();

    completed = 0;
    lastTime = System.currentTimeMillis();
    switch (operation) {
      case GRADIENT_MAGNITUDE:
        processDuration = stackSize * (width + 2 * height);
        break;
      case GRADIENT_DIRECTION:
        processDuration = stackSize * (width + 2 * height);
        break;
      case LAPLACIAN:
        processDuration = stackSize * (width + 2 * height);
        break;
      case LARGEST_HESSIAN:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      case SMALLEST_HESSIAN:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      case HESSIAN_ORIENTATION:
        processDuration = stackSize * (2 * width + 3 * height);
        break;
      default:
        throw new IllegalArgumentException("Invalid operation");
    }
  } /* end setupProgressBar */
 void interpolate() {
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("Interpolate");
     return;
   }
   if (roi.getType() == Roi.POINT) return;
   if (IJ.isMacro() && Macro.getOptions() == null) Macro.setOptions("interval=1");
   GenericDialog gd = new GenericDialog("Interpolate");
   gd.addNumericField("Interval:", 1.0, 1, 4, "pixel");
   gd.addCheckbox("Smooth", IJ.isMacro() ? false : smooth);
   gd.showDialog();
   if (gd.wasCanceled()) return;
   double interval = gd.getNextNumber();
   smooth = gd.getNextBoolean();
   Undo.setup(Undo.ROI, imp);
   FloatPolygon poly = roi.getInterpolatedPolygon(interval, smooth);
   int t = roi.getType();
   int type = roi.isLine() ? Roi.FREELINE : Roi.FREEROI;
   if (t == Roi.POLYGON && interval > 1.0) type = Roi.POLYGON;
   if ((t == Roi.RECTANGLE || t == Roi.OVAL || t == Roi.FREEROI) && interval >= 5.0)
     type = Roi.POLYGON;
   if ((t == Roi.LINE || t == Roi.FREELINE) && interval >= 5.0) type = Roi.POLYLINE;
   if (t == Roi.POLYLINE && interval >= 1.0) type = Roi.POLYLINE;
   ImageCanvas ic = imp.getCanvas();
   if (poly.npoints <= 150 && ic != null && ic.getMagnification() >= 12.0)
     type = roi.isLine() ? Roi.POLYLINE : Roi.POLYGON;
   Roi p = new PolygonRoi(poly, type);
   if (roi.getStroke() != null) p.setStrokeWidth(roi.getStrokeWidth());
   p.setStrokeColor(roi.getStrokeColor());
   p.setName(roi.getName());
   transferProperties(roi, p);
   imp.setRoi(p);
 }
 void lineToArea(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null || !roi.isLine()) {
     IJ.error("Line to Area", "Line selection required");
     return;
   }
   if (roi.getType() == Roi.LINE && roi.getStrokeWidth() == 1) {
     IJ.error("Line to Area", "Straight line width must be > 1");
     return;
   }
   ImageProcessor ip2 = new ByteProcessor(imp.getWidth(), imp.getHeight());
   ip2.setColor(255);
   if (roi.getType() == Roi.LINE) ip2.fillPolygon(roi.getPolygon());
   else {
     roi.drawPixels(ip2);
     // BufferedImage bi = new BufferedImage(imp.getWidth(), imp.getHeight(),
     // BufferedImage.TYPE_BYTE_GRAY);
     // Graphics g = bi.getGraphics();
     // Roi roi2 = (Roi)roi.clone();
     // roi2.setStrokeColor(Color.white);
     // roi2.drawOverlay(g);
     // ip2 = new ByteProcessor(bi);
   }
   // new ImagePlus("ip2", ip2.duplicate()).show();
   ip2.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
   ThresholdToSelection tts = new ThresholdToSelection();
   Roi roi2 = tts.convert(ip2);
   imp.setRoi(roi2);
   Roi.previousRoi = (Roi) roi.clone();
 }
Beispiel #4
0
  /** Generate output image whose type is same as input image. */
  private ImagePlus makeOutputImage(ImagePlus imp, FloatProcessor fp, int ptype) {
    int width = imp.getWidth();
    int height = imp.getHeight();
    float[] pixels = (float[]) fp.getPixels();
    ImageProcessor oip = null;

    // Create output image consistent w/ type of input image.
    int size = pixels.length;
    switch (ptype) {
      case BYTE_TYPE:
        oip = imp.getProcessor().createProcessor(width, height);
        byte[] pixels8 = (byte[]) oip.getPixels();
        for (int i = 0; i < size; i++) pixels8[i] = (byte) pixels[i];
        break;
      case SHORT_TYPE:
        oip = imp.getProcessor().createProcessor(width, height);
        short[] pixels16 = (short[]) oip.getPixels();
        for (int i = 0; i < size; i++) pixels16[i] = (short) pixels[i];
        break;
      case FLOAT_TYPE:
        oip = new FloatProcessor(width, height, pixels, null);
        break;
    }

    // Adjust for display.
    // Calling this on non-ByteProcessors ensures image
    // processor is set up to correctly display image.
    oip.resetMinAndMax();

    // Create new image plus object. Don't use
    // ImagePlus.createImagePlus here because there may be
    // attributes of input image that are not appropriate for
    // projection.
    return new ImagePlus(makeTitle(), oip);
  }
Beispiel #5
0
 // Added by Marcel Boeglin 2013.09.22
 private Overlay projectHyperStackRois(Overlay overlay) {
   if (overlay == null) return null;
   int t1 = imp.getFrame();
   int channels = projImage.getNChannels();
   int slices = 1;
   int frames = projImage.getNFrames();
   Overlay overlay2 = new Overlay();
   Roi roi;
   int c, z, t;
   int size = channels * slices * frames;
   for (Roi r : overlay.toArray()) {
     c = r.getCPosition();
     z = r.getZPosition();
     t = r.getTPosition();
     roi = (Roi) r.clone();
     if (size == channels) { // current time frame
       if (z >= startSlice && z <= stopSlice && t == t1 || c == 0) {
         roi.setPosition(c);
         overlay2.add(roi);
       }
     } else if (size == frames * channels) { // all time frames
       if (z >= startSlice && z <= stopSlice) roi.setPosition(c, 1, t);
       else if (z == 0) roi.setPosition(c, 0, t);
       else continue;
       overlay2.add(roi);
     }
   }
   return overlay2;
 }
Beispiel #6
0
 void createEllipse(ImagePlus imp) {
   IJ.showStatus("Fitting ellipse");
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("Fit Ellipse");
     return;
   }
   if (roi.isLine()) {
     IJ.error("Fit Ellipse", "\"Fit Ellipse\" does not work with line selections");
     return;
   }
   ImageProcessor ip = imp.getProcessor();
   ip.setRoi(roi);
   int options = Measurements.CENTROID + Measurements.ELLIPSE;
   ImageStatistics stats = ImageStatistics.getStatistics(ip, options, null);
   double dx = stats.major * Math.cos(stats.angle / 180.0 * Math.PI) / 2.0;
   double dy = -stats.major * Math.sin(stats.angle / 180.0 * Math.PI) / 2.0;
   double x1 = stats.xCentroid - dx;
   double x2 = stats.xCentroid + dx;
   double y1 = stats.yCentroid - dy;
   double y2 = stats.yCentroid + dy;
   double aspectRatio = stats.minor / stats.major;
   imp.killRoi();
   imp.setRoi(new EllipseRoi(x1, y1, x2, y2, aspectRatio));
 }
Beispiel #7
0
 boolean validDialogValues() {
   Calibration cal = imp.getCalibration();
   double pw = cal.pixelWidth, ph = cal.pixelHeight;
   if (width / pw < 1 || height / ph < 1) return false;
   if (xRoi / pw > imp.getWidth() || yRoi / ph > imp.getHeight()) return false;
   return true;
 }
 void updateSliceSummary() {
   int slices = imp.getStackSize();
   float[] areas = rt.getColumn(ResultsTable.AREA);
   if (areas == null) areas = new float[0];
   String label = imp.getTitle();
   if (slices > 1) {
     label = imp.getStack().getShortSliceLabel(slice);
     label = label != null && !label.equals("") ? label : "" + slice;
   }
   String aLine = null;
   double sum = 0.0;
   int start = areas.length - particleCount;
   if (start < 0) return;
   for (int i = start; i < areas.length; i++) sum += areas[i];
   int places = Analyzer.getPrecision();
   Calibration cal = imp.getCalibration();
   String total = "\t" + ResultsTable.d2s(sum, places);
   String average = "\t" + ResultsTable.d2s(sum / particleCount, places);
   String fraction = "\t" + ResultsTable.d2s(sum * 100.0 / totalArea, 1);
   aLine = label + "\t" + particleCount + total + average + fraction;
   aLine = addMeans(aLine, areas.length > 0 ? start : -1);
   if (slices == 1) {
     Frame frame = WindowManager.getFrame("Summary");
     if (frame != null && (frame instanceof TextWindow) && summaryHdr.equals(prevHdr))
       tw = (TextWindow) frame;
   }
   if (tw == null) {
     String title = slices == 1 ? "Summary" : "Summary of " + imp.getTitle();
     tw = new TextWindow(title, summaryHdr, aLine, 450, 300);
     prevHdr = summaryHdr;
   } else tw.append(aLine);
 }
Beispiel #9
0
 void showDialog() {
   int width = imp.getWidth();
   int height = imp.getHeight();
   Calibration cal = imp.getCalibration();
   int places;
   if (cal.scaled()) {
     pixelWidth = cal.pixelWidth;
     pixelHeight = cal.pixelHeight;
     units = cal.getUnits();
     places = 2;
   } else {
     pixelWidth = 1.0;
     pixelHeight = 1.0;
     units = "pixels";
     places = 0;
   }
   if (areaPerPoint == 0.0)
     areaPerPoint =
         (width * cal.pixelWidth * height * cal.pixelHeight) / 81.0; // default to 9x9 grid
   ImageWindow win = imp.getWindow();
   GenericDialog gd = new GenericDialog("Grid...");
   gd.addChoice("Grid Type:", types, type);
   gd.addNumericField("Area per Point:", areaPerPoint, places, 6, units + "^2");
   gd.addChoice("Color:", colors, color);
   gd.addCheckbox("Random Offset", randomOffset);
   gd.addDialogListener(this);
   gd.showDialog();
   if (gd.wasCanceled()) showGrid(null);
 }
Beispiel #10
0
 private String getExifData(ImagePlus imp) {
   FileInfo fi = imp.getOriginalFileInfo();
   if (fi == null) return null;
   String directory = fi.directory;
   String name = fi.fileName;
   if (directory == null) return null;
   if ((name == null || name.equals("")) && imp.getStack().isVirtual())
     name = imp.getStack().getSliceLabel(imp.getCurrentSlice());
   if (name == null || !(name.endsWith("jpg") || name.endsWith("JPG"))) return null;
   String path = directory + name;
   String metadata = null;
   try {
     Class c = IJ.getClassLoader().loadClass("Exif_Reader");
     if (c == null) return null;
     String methodName = "getMetadata";
     Class[] argClasses = new Class[1];
     argClasses[0] = methodName.getClass();
     Method m = c.getMethod("getMetadata", argClasses);
     Object[] args = new Object[1];
     args[0] = path;
     Object obj = m.invoke(null, args);
     metadata = obj != null ? obj.toString() : null;
   } catch (Exception e) {
     return null;
   }
   if (metadata != null && !metadata.startsWith("Error:")) return metadata;
   else return null;
 }
 public int setup(String arg, ImagePlus imp) {
   this.arg = arg;
   this.imp = imp;
   IJ.register(ParticleAnalyzer.class);
   if (imp == null) {
     IJ.noImage();
     return DONE;
   }
   if (imp.getBitDepth() == 24 && !isThresholdedRGB(imp)) {
     IJ.error(
         "Particle Analyzer",
         "RGB images must be thresholded using\n" + "Image>Adjust>Color Threshold.");
     return DONE;
   }
   if (!showDialog()) return DONE;
   int baseFlags = DOES_ALL + NO_CHANGES + NO_UNDO;
   int flags = IJ.setupDialog(imp, baseFlags);
   processStack = (flags & DOES_STACKS) != 0;
   slice = 0;
   saveRoi = imp.getRoi();
   if (saveRoi != null && saveRoi.getType() != Roi.RECTANGLE && saveRoi.isArea())
     polygon = saveRoi.getPolygon();
   imp.startTiming();
   nextFontSize = defaultFontSize;
   nextLineWidth = 1;
   return flags;
 }
Beispiel #12
0
 void remove() {
   ImagePlus imp = WindowManager.getCurrentImage();
   if (imp != null) imp.setOverlay(null);
   overlay2 = null;
   RoiManager rm = RoiManager.getInstance();
   if (rm != null) rm.runCommand("show none");
 }
Beispiel #13
0
 void lineToArea(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null || !roi.isLine()) {
     IJ.error("Line to Area", "Line selection required");
     return;
   }
   Undo.setup(Undo.ROI, imp);
   Roi roi2 = null;
   if (roi.getType() == Roi.LINE) {
     double width = roi.getStrokeWidth();
     if (width <= 1.0) roi.setStrokeWidth(1.0000001);
     FloatPolygon p = roi.getFloatPolygon();
     roi.setStrokeWidth(width);
     roi2 = new PolygonRoi(p, Roi.POLYGON);
     roi2.setDrawOffset(roi.getDrawOffset());
   } else {
     ImageProcessor ip2 = new ByteProcessor(imp.getWidth(), imp.getHeight());
     ip2.setColor(255);
     roi.drawPixels(ip2);
     // new ImagePlus("ip2", ip2.duplicate()).show();
     ip2.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
     ThresholdToSelection tts = new ThresholdToSelection();
     roi2 = tts.convert(ip2);
   }
   transferProperties(roi, roi2);
   roi2.setStrokeWidth(0);
   Color c = roi2.getStrokeColor();
   if (c != null) // remove any transparency
   roi2.setStrokeColor(new Color(c.getRed(), c.getGreen(), c.getBlue()));
   imp.setRoi(roi2);
   Roi.previousRoi = (Roi) roi.clone();
 }
Beispiel #14
0
  /**
   * Prepare for processing; also called at the very end with argument 'final' to show any newly
   * created output image.
   */
  public int setup(String arg, ImagePlus imp) {
    if (arg.equals("final")) {
      showOutput();
      return DONE;
    }
    this.imp = imp;
    // 'arg' is processing type; default is 'EDM' (0)
    if (arg.equals("watershed")) {
      processType = WATERSHED;
      flags += KEEP_THRESHOLD;
    } else if (arg.equals("points")) processType = UEP;
    else if (arg.equals("voronoi")) processType = VORONOI;

    // output type
    if (processType != WATERSHED) // Watershed always has output BYTE_OVERWRITE=0
    outImageType = outputType; // otherwise use the static variable from setOutputType
    if (outImageType != BYTE_OVERWRITE) flags |= NO_CHANGES;

    // check image and prepare
    if (imp != null) {
      ImageProcessor ip = imp.getProcessor();
      if (!ip.isBinary()) {
        IJ.error("8-bit binary image (0 and 255) required.");
        return DONE;
      }
      ip.resetRoi();
      // processing routines assume background=0; image may be otherwise
      boolean invertedLut = imp.isInvertedLut();
      background255 =
          (invertedLut && Prefs.blackBackground) || (!invertedLut && !Prefs.blackBackground);
    }
    return flags;
  } // public int setup
Beispiel #15
0
 void fitSpline() {
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("Spline");
     return;
   }
   int type = roi.getType();
   boolean segmentedSelection = type == Roi.POLYGON || type == Roi.POLYLINE;
   if (!(segmentedSelection
       || type == Roi.FREEROI
       || type == Roi.TRACED_ROI
       || type == Roi.FREELINE)) {
     IJ.error("Spline", "Polygon or polyline selection required");
     return;
   }
   if (roi instanceof EllipseRoi) return;
   PolygonRoi p = (PolygonRoi) roi;
   if (!segmentedSelection) {
     if (p.subPixelResolution()) p = trimFloatPolygon(p, p.getUncalibratedLength());
     else p = trimPolygon(p, p.getUncalibratedLength());
   }
   String options = Macro.getOptions();
   if (options != null && options.indexOf("straighten") != -1) p.fitSplineForStraightening();
   else if (options != null && options.indexOf("remove") != -1) p.removeSplineFit();
   else p.fitSpline();
   imp.draw();
   LineWidthAdjuster.update();
 }
Beispiel #16
0
 /**
  * Creates a dialog box, allowing the user to enter the requested width, height, x & y
  * coordinates, slice number for a Region Of Interest, option for oval, and option for whether x &
  * y coordinates to be centered.
  */
 void showDialog() {
   Calibration cal = imp.getCalibration();
   int digits = 0;
   if (scaledUnits && cal.scaled()) digits = 2;
   Roi roi = imp.getRoi();
   if (roi == null) drawRoi();
   GenericDialog gd = new GenericDialog("Specify");
   gd.addNumericField("Width:", width, digits);
   gd.addNumericField("Height:", height, digits);
   gd.addNumericField("X coordinate:", xRoi, digits);
   gd.addNumericField("Y coordinate:", yRoi, digits);
   if (stackSize > 1) gd.addNumericField("Slice:", iSlice, 0);
   gd.addCheckbox("Oval", oval);
   gd.addCheckbox("Constrain square/circle", square);
   gd.addCheckbox("Centered", centered);
   if (cal.scaled()) {
     boolean unitsMatch = cal.getXUnit().equals(cal.getYUnit());
     String units = unitsMatch ? cal.getUnits() : cal.getXUnit() + " x " + cal.getYUnit();
     gd.addCheckbox("Scaled units (" + units + ")", scaledUnits);
   }
   fields = gd.getNumericFields();
   gd.addDialogListener(this);
   gd.showDialog();
   if (gd.wasCanceled()) {
     if (roi == null) imp.deleteRoi();
     else // *ALWAYS* restore initial ROI when cancelled
     imp.setRoi(roi);
   }
 }
 /**
  * Saves statistics for one particle in a results table. This is a method subclasses may want to
  * override.
  */
 protected void saveResults(ImageStatistics stats, Roi roi) {
   analyzer.saveResults(stats, roi);
   if (recordStarts) {
     rt.addValue("XStart", stats.xstart);
     rt.addValue("YStart", stats.ystart);
   }
   if (addToManager) {
     if (roiManager == null) {
       if (Macro.getOptions() != null && Interpreter.isBatchMode())
         roiManager = Interpreter.getBatchModeRoiManager();
       if (roiManager == null) {
         Frame frame = WindowManager.getFrame("ROI Manager");
         if (frame == null) IJ.run("ROI Manager...");
         frame = WindowManager.getFrame("ROI Manager");
         if (frame == null || !(frame instanceof RoiManager)) {
           addToManager = false;
           return;
         }
         roiManager = (RoiManager) frame;
       }
       if (resetCounter) roiManager.runCommand("reset");
     }
     if (imp.getStackSize() > 1) roi.setPosition(imp.getCurrentSlice());
     if (lineWidth != 1) roi.setStrokeWidth(lineWidth);
     roiManager.add(imp, roi, rt.getCounter());
   }
   if (showResults) rt.addResults();
 }
 private ImagePlus duplicateImage(ImageProcessor iProcessor) {
   int w = iProcessor.getWidth();
   int h = iProcessor.getHeight();
   ImagePlus iPlus = NewImage.createByteImage("Image", w, h, 1, NewImage.FILL_BLACK);
   ImageProcessor imageProcessor = iPlus.getProcessor();
   imageProcessor.copyBits(iProcessor, 0, 0, Blitter.COPY);
   return iPlus;
 }
Beispiel #19
0
 /**
  * Builds dialog to query users for projection parameters.
  *
  * @param start starting slice to display
  * @param stop last slice
  */
 protected GenericDialog buildControlDialog(int start, int stop) {
   GenericDialog gd = new GenericDialog("ZProjection", IJ.getInstance());
   gd.addNumericField("Start slice:", startSlice, 0 /*digits*/);
   gd.addNumericField("Stop slice:", stopSlice, 0 /*digits*/);
   gd.addChoice("Projection type", METHODS, METHODS[method]);
   if (isHyperstack && imp.getNFrames() > 1 && imp.getNSlices() > 1)
     gd.addCheckbox("All time frames", allTimeFrames);
   return gd;
 }
  void Bernsen(ImagePlus imp, int radius, double par1, double par2, boolean doIwhite) {
    // Bernsen recommends WIN_SIZE = 31 and CONTRAST_THRESHOLD = 15.
    //  1) Bernsen J. (1986) "Dynamic Thresholding of Grey-Level Images"
    //    Proc. of the 8th Int. Conf. on Pattern Recognition, pp. 1251-1255
    //  2) Sezgin M. and Sankur B. (2004) "Survey over Image Thresholding
    //   Techniques and Quantitative Performance Evaluation" Journal of
    //   Electronic Imaging, 13(1): 146-165
    //  http://citeseer.ist.psu.edu/sezgin04survey.html
    // Ported to ImageJ plugin from E Celebi's fourier_0.8 routines
    // This version uses a circular local window, instead of a rectagular one
    ImagePlus Maximp, Minimp;
    ImageProcessor ip = imp.getProcessor(), ipMax, ipMin;
    int contrast_threshold = 15;
    int local_contrast;
    int mid_gray;
    byte object;
    byte backg;
    int temp;

    if (par1 != 0) {
      IJ.log("Bernsen: changed contrast_threshold from :" + contrast_threshold + "  to:" + par1);
      contrast_threshold = (int) par1;
    }

    if (doIwhite) {
      object = (byte) 0xff;
      backg = (byte) 0;
    } else {
      object = (byte) 0;
      backg = (byte) 0xff;
    }

    Maximp = duplicateImage(ip);
    ipMax = Maximp.getProcessor();
    RankFilters rf = new RankFilters();
    rf.rank(ipMax, radius, rf.MAX); // Maximum
    // Maximp.show();
    Minimp = duplicateImage(ip);
    ipMin = Minimp.getProcessor();
    rf.rank(ipMin, radius, rf.MIN); // Minimum
    // Minimp.show();
    byte[] pixels = (byte[]) ip.getPixels();
    byte[] max = (byte[]) ipMax.getPixels();
    byte[] min = (byte[]) ipMin.getPixels();

    for (int i = 0; i < pixels.length; i++) {
      local_contrast = (int) ((max[i] & 0xff) - (min[i] & 0xff));
      mid_gray = (int) ((min[i] & 0xff) + (max[i] & 0xff)) / 2;
      temp = (int) (pixels[i] & 0x0000ff);
      if (local_contrast < contrast_threshold)
        pixels[i] = (mid_gray >= 128) ? object : backg; // Low contrast region
      else pixels[i] = (temp >= mid_gray) ? object : backg;
    }
    // imp.updateAndDraw();
    return;
  }
Beispiel #21
0
 void toBoundingBox(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("To Bounding Box");
     return;
   }
   Rectangle r = roi.getBounds();
   imp.killRoi();
   imp.setRoi(new Roi(r.x, r.y, r.width, r.height));
 }
Beispiel #22
0
 void hide() {
   ImagePlus imp = IJ.getImage();
   Overlay overlay = imp.getOverlay();
   if (overlay != null) {
     overlay2 = overlay;
     imp.setOverlay(null);
   }
   RoiManager rm = RoiManager.getInstance();
   if (rm != null) rm.runCommand("show none");
 }
Beispiel #23
0
  /** Performs actual projection using specified method. */
  public void doProjection() {
    if (imp == null) return;
    sliceCount = 0;
    if (method < AVG_METHOD || method > MEDIAN_METHOD) method = AVG_METHOD;
    for (int slice = startSlice; slice <= stopSlice; slice += increment) sliceCount++;
    if (method == MEDIAN_METHOD) {
      projImage = doMedianProjection();
      return;
    }

    // Create new float processor for projected pixels.
    FloatProcessor fp = new FloatProcessor(imp.getWidth(), imp.getHeight());
    ImageStack stack = imp.getStack();
    RayFunction rayFunc = getRayFunction(method, fp);
    if (IJ.debugMode == true) {
      IJ.log("\nProjecting stack from: " + startSlice + " to: " + stopSlice);
    }

    // Determine type of input image. Explicit determination of
    // processor type is required for subsequent pixel
    // manipulation.  This approach is more efficient than the
    // more general use of ImageProcessor's getPixelValue and
    // putPixel methods.
    int ptype;
    if (stack.getProcessor(1) instanceof ByteProcessor) ptype = BYTE_TYPE;
    else if (stack.getProcessor(1) instanceof ShortProcessor) ptype = SHORT_TYPE;
    else if (stack.getProcessor(1) instanceof FloatProcessor) ptype = FLOAT_TYPE;
    else {
      IJ.error("Z Project", "Non-RGB stack required");
      return;
    }

    // Do the projection.
    for (int n = startSlice; n <= stopSlice; n += increment) {
      IJ.showStatus("ZProjection " + color + ": " + n + "/" + stopSlice);
      IJ.showProgress(n - startSlice, stopSlice - startSlice);
      projectSlice(stack.getPixels(n), rayFunc, ptype);
    }

    // Finish up projection.
    if (method == SUM_METHOD) {
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else if (method == SD_METHOD) {
      rayFunc.postProcess();
      fp.resetMinAndMax();
      projImage = new ImagePlus(makeTitle(), fp);
    } else {
      rayFunc.postProcess();
      projImage = makeOutputImage(imp, fp, ptype);
    }

    if (projImage == null) IJ.error("Z Project", "Error computing projection.");
  }
Beispiel #24
0
 void invert(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null || !roi.isArea()) {
     IJ.error("Inverse", "Area selection required");
     return;
   }
   ShapeRoi s1, s2;
   if (roi instanceof ShapeRoi) s1 = (ShapeRoi) roi;
   else s1 = new ShapeRoi(roi);
   s2 = new ShapeRoi(new Roi(0, 0, imp.getWidth(), imp.getHeight()));
   imp.setRoi(s1.xor(s2));
 }
Beispiel #25
0
 public int setup(String arg, ImagePlus imp) {
   this.imp = imp;
   if (imp != null) {
     bitDepth = imp.getBitDepth();
     Roi roi = imp.getRoi();
     Rectangle r = roi != null ? roi.getBounds() : null;
     canEnlarge =
         r == null
             || (r.x == 0 && r.y == 0 && r.width == imp.getWidth() && r.height == imp.getHeight());
   }
   return flags;
 }
Beispiel #26
0
 private boolean ignoreArrowKeys(ImagePlus imp) {
   Frame frame = WindowManager.getFrontWindow();
   String title = frame.getTitle();
   if (title != null && title.equals("ROI Manager")) return true;
   // Control Panel?
   if (frame != null && frame instanceof javax.swing.JFrame) return true;
   ImageWindow win = imp.getWindow();
   // LOCI Data Browser window?
   if (imp.getStackSize() > 1 && win != null && win.getClass().getName().startsWith("loci"))
     return true;
   return false;
 }
Beispiel #27
0
 void toBoundingBox(ImagePlus imp) {
   Roi roi = imp.getRoi();
   if (roi == null) {
     noRoi("To Bounding Box");
     return;
   }
   Undo.setup(Undo.ROI, imp);
   Rectangle r = roi.getBounds();
   imp.deleteRoi();
   Roi roi2 = new Roi(r.x, r.y, r.width, r.height);
   transferProperties(roi, roi2);
   imp.setRoi(roi2);
 }
Beispiel #28
0
 /**
  * Called by the PlugInFilterRunner after setup. Asks the user in case of a stack and prepares a
  * separate ouptut stack if required
  */
 public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) {
   this.pfr = pfr;
   int width = imp.getWidth();
   int height = imp.getHeight();
   // ask whether to process all slices of stack & prepare stack
   // (if required) for writing into it in parallel threads
   flags = IJ.setupDialog(imp, flags);
   if ((flags & DOES_STACKS) != 0 && outImageType != BYTE_OVERWRITE) {
     outStack = new ImageStack(width, height, imp.getStackSize());
     maxFinder.setNPasses(imp.getStackSize());
   }
   return flags;
 } // public int showDialog
Beispiel #29
0
 void lineWidth() {
   int width = (int) IJ.getNumber("Line Width:", Line.getWidth());
   if (width == IJ.CANCELED) return;
   Line.setWidth(width);
   LineWidthAdjuster.update();
   ImagePlus imp = WindowManager.getCurrentImage();
   if (imp != null && imp.isProcessor()) {
     ImageProcessor ip = imp.getProcessor();
     ip.setLineWidth(Line.getWidth());
     Roi roi = imp.getRoi();
     if (roi != null && roi.isLine()) imp.draw();
   }
 }
 boolean setThresholdLevels(ImagePlus imp, ImageProcessor ip) {
   double t1 = ip.getMinThreshold();
   double t2 = ip.getMaxThreshold();
   boolean invertedLut = imp.isInvertedLut();
   boolean byteImage = ip instanceof ByteProcessor;
   if (ip instanceof ShortProcessor) imageType = SHORT;
   else if (ip instanceof FloatProcessor) imageType = FLOAT;
   else imageType = BYTE;
   if (t1 == ImageProcessor.NO_THRESHOLD) {
     ImageStatistics stats = imp.getStatistics();
     if (imageType != BYTE || (stats.histogram[0] + stats.histogram[255] != stats.pixelCount)) {
       IJ.error(
           "Particle Analyzer",
           "A thresholded image or 8-bit binary image is\n"
               + "required. Threshold levels can be set using\n"
               + "the Image->Adjust->Threshold tool.");
       canceled = true;
       return false;
     }
     boolean threshold255 = invertedLut;
     if (Prefs.blackBackground) threshold255 = !threshold255;
     if (threshold255) {
       level1 = 255;
       level2 = 255;
       fillColor = 64;
     } else {
       level1 = 0;
       level2 = 0;
       fillColor = 192;
     }
   } else {
     level1 = t1;
     level2 = t2;
     if (imageType == BYTE) {
       if (level1 > 0) fillColor = 0;
       else if (level2 < 255) fillColor = 255;
     } else if (imageType == SHORT) {
       if (level1 > 0) fillColor = 0;
       else if (level2 < 65535) fillColor = 65535;
     } else if (imageType == FLOAT) fillColor = -Float.MAX_VALUE;
     else return false;
   }
   imageType2 = imageType;
   if (redirectIP != null) {
     if (redirectIP instanceof ShortProcessor) imageType2 = SHORT;
     else if (redirectIP instanceof FloatProcessor) imageType2 = FLOAT;
     else if (redirectIP instanceof ColorProcessor) imageType2 = RGB;
     else imageType2 = BYTE;
   }
   return true;
 }