public Collection<NamedRegion> parseProbes() throws IOException {
double ethreshold = 1.0e-3;
Parser<BlastTabEntry> parser =
new Parser<BlastTabEntry>(blasttab, new BlastTabEntry.ParsingMapper());
Iterator<BlastTabEntry> itr =
new FilterIterator<BlastTabEntry, BlastTabEntry>(
new BlastTabEntry.ExpectedScoreFilter(ethreshold), parser);
Map<String, Set<Region>> primerHits = new TreeMap<String, Set<Region>>();
Set<String> primerNames = new TreeSet<String>();
while (itr.hasNext()) {
BlastTabEntry entry = itr.next();
NamedRegion r = createNamedRegion(entry);
if (!primerHits.containsKey(entry.getQuery())) {
primerHits.put(entry.getQuery(), new HashSet<Region>());
}
primerHits.get(entry.getQuery()).add(r);
primerNames.add(removePrimerLR(entry.getQuery()));
}
LinkedList<NamedRegion> probes = new LinkedList<NamedRegion>();
for (String primerName : primerNames) {
Pair<Region, Region> hits = findProbePair(primerName, primerHits);
if (hits != null) {
Region left = hits.getFirst(), right = hits.getLast();
int start = Math.min(left.getStart(), right.getStart());
int end = Math.max(left.getEnd(), right.getEnd());
NamedRegion probe = new NamedRegion(genome, left.getChrom(), start, end, primerName);
probes.addLast(probe);
}
}
return probes;
}
public void loadNetwork() throws IOException {
logger.log(Level.INFO, "Loading metabolism network...");
File file = props.getNetworkFile();
Mapper<String, MetabolicEntry> entryMapper = new MetabolicEntry.MetabolicMapper();
Parser<MetabolicEntry> parser = new Parser<MetabolicEntry>(file, entryMapper);
while (parser.hasNext()) {
MetabolicEntry entry = parser.next();
entries.add(entry);
Rxn rxn =
new Rxn(
props,
entry.getReaction(),
entry.getAbbreviation(),
entry.getReactionName(),
entry.getORF());
reactions.put(entry.getAbbreviation(), rxn);
locations.add(rxn.getLocation());
LogicalORFTree lot = new LogicalORFTree(entry.getORF());
ORFSet os = new ORFSet(entry.getORF());
totalORFs.addAll(os.getORFs());
orfSets.put(entry.getAbbreviation(), lot);
}
logger.log(Level.FINE, String.format("Loaded %d entries.", entries.size()));
abbrevs = new MetabolismAbbreviations(props);
abbrevs.loadAbbreviations();
logger.log(Level.FINEST, "Loaded abbrevations.");
}