/**
* this method assigns values for each new condition, from the previously created 8and set-up) one
*
* @param newCondition
*/
private void initNewCondition(PlateCondition newCondition) {
PlateCondition previousCondition = plateConditionBindingList.get(previousConditionIndex);
// set the name
newCondition.setName("Condition " + ++conditionIndex);
// set the cell line (the same as the previous condition)
CellLine cellLine = previousCondition.getCellLine();
CellLine newCellLine =
new CellLine(
cellLine.getSeedingTime(),
cellLine.getSeedingDensity(),
cellLine.getGrowthMedium(),
cellLine.getSerumConcentration(),
cellLine.getCellLineType(),
cellLine.getSerum());
newCondition.setCellLine(newCellLine);
newCellLine.setPlateCondition(newCondition);
// set assay medium (another object, but with the same parameters as previous condition)
String medium =
mediumBindingList.get(
treatmentsController.getTreatmentsPanel().getAssayMediumComboBox().getSelectedIndex());
String serum =
serumBindingList.get(
treatmentsController.getTreatmentsPanel().getSerumComboBox().getSelectedIndex());
Double serumConcentration =
Double.parseDouble(
treatmentsController.getTreatmentsPanel().getSerumConcentrationTextField().getText());
Double volume =
Double.parseDouble(
treatmentsController.getTreatmentsPanel().getMediumVolumeTextField().getText());
AssayMedium assayMedium = new AssayMedium(medium, serum, serumConcentration, volume);
newCondition.setAssayMedium(assayMedium);
assayMedium.setPlateCondition(newCondition);
// set assay and ecm (get the values according to the last selected ones)
Ecm ecm = new Ecm();
Assay assay = null;
BottomMatrix bottomMatrix = null;
// need to set different values according to matrix dimension: 2D or 3D or 2.5D
switch (assayEcmController
.getMatrixDimensionBindingList()
.get(setupConditionsPanel.getEcmDimensionComboBox().getSelectedIndex())
.getDimension()) {
case "2D":
// set assay
assay =
assayEcmController
.getAssay2DBindingList()
.get(assayEcmController.getAssayEcm2DPanel().getAssayComboBox().getSelectedIndex());
// 2D matrix: set ecm 2D fields
ecm.setEcmComposition(
assayEcmController
.getEcm2DCompositionBindingList()
.get(
assayEcmController
.getAssayEcm2DPanel()
.getCompositionComboBox()
.getSelectedIndex()));
ecm.setConcentration(
Double.parseDouble(
assayEcmController.getAssayEcm2DPanel().getConcentrationTextField().getText()));
ecm.setVolume(
Double.parseDouble(
assayEcmController.getAssayEcm2DPanel().getVolumeTextField().getText()));
ecm.setCoatingTime(
assayEcmController.getAssayEcm2DPanel().getCoatingTimeTextField().getText());
ecm.setCoatingTemperature(
assayEcmController.getAssayEcm2DPanel().getCoatingTemperatureTextField().getText());
ecm.setVolumeUnit(assayEcmController.getAssayEcm2DPanel().getVolumeUnitLabel().getText());
ecm.setConcentrationUnit(
assayEcmController
.getAssayEcm2DPanel()
.getConcentrationUnitOfMeasure()
.getSelectedItem()
.toString());
break;
case "3D":
// set assay
assay =
assayEcmController
.getAssay3DBindingList()
.get(assayEcmController.getAssayEcm3DPanel().getAssayComboBox().getSelectedIndex());
// 3D matrix: set ecm 3D fields
ecm.setEcmComposition(
assayEcmController
.getEcm3DCompositionBindingList()
.get(
assayEcmController
.getAssayEcm3DPanel()
.getCompositionComboBox()
.getSelectedIndex()));
ecm.setEcmDensity(
assayEcmController
.getEcmDensityBindingList()
.get(
assayEcmController
.getAssayEcm3DPanel()
.getDensityComboBox()
.getSelectedIndex()));
// bottom matrix
bottomMatrix =
assayEcmController
.getBottomMatrixBindingList()
.get(
assayEcmController
.getAssayEcm3DPanel()
.getBottomMatrixTypeComboBox()
.getSelectedIndex());
ecm.setBottomMatrix(bottomMatrix);
switch (bottomMatrix.getType()) {
case "gel":
// both top and bottom matrix volumes
String text =
assayEcmController.getAssayEcm3DPanel().getTopMatrixVolumeTextField().getText();
if (!text.isEmpty()) {
ecm.setTopMatrixVolume(Double.parseDouble(text));
} else {
ecm.setTopMatrixVolume(40.0);
}
ecm.setBottomMatrixVolume(
Double.parseDouble(
assayEcmController
.getAssayEcm3DPanel()
.getBottomMatrixVolumeTextField()
.getText()));
break;
case "thin gel coating":
// top matrix but no bottom matrix volume
ecm.setTopMatrixVolume(
Double.parseDouble(
assayEcmController
.getAssayEcm3DPanel()
.getTopMatrixVolumeTextField()
.getText()));
break;
}
ecm.setPolymerisationTime(
assayEcmController.getAssayEcm3DPanel().getPolymerizationTimeTextField().getText());
ecm.setPolymerisationTemperature(
assayEcmController
.getAssayEcm3DPanel()
.getPolymerizationTemperatureTextField()
.getText());
ecm.setPolymerisationPh(
assayEcmController
.getPolymerisationPhBindingList()
.get(
assayEcmController
.getAssayEcm3DPanel()
.getPolymerizationPhComboBox()
.getSelectedIndex()));
break;
case "2.5D":
// set assay
assay =
assayEcmController
.getAssay25DBindingList()
.get(
assayEcmController.getAssayEcm25DPanel().getAssayComboBox().getSelectedIndex());
// 3D matrix: set ecm 2.5D fields
ecm.setEcmComposition(
assayEcmController
.getEcm25DCompositionBindingList()
.get(
assayEcmController
.getAssayEcm25DPanel()
.getCompositionComboBox()
.getSelectedIndex()));
ecm.setEcmDensity(
assayEcmController
.getEcmDensityBindingList()
.get(
assayEcmController
.getAssayEcm25DPanel()
.getDensityComboBox()
.getSelectedIndex()));
// bottom matrix
bottomMatrix =
assayEcmController
.getBottomMatrixBindingList()
.get(
assayEcmController
.getAssayEcm25DPanel()
.getBottomMatrixTypeComboBox()
.getSelectedIndex());
ecm.setBottomMatrix(bottomMatrix);
switch (bottomMatrix.getType()) {
case "gel":
// bottom matrix volume
ecm.setBottomMatrixVolume(
Double.parseDouble(
assayEcmController
.getAssayEcm25DPanel()
.getBottomMatrixVolumeTextField()
.getText()));
break;
}
ecm.setPolymerisationTime(
assayEcmController.getAssayEcm25DPanel().getPolymerizationTimeTextField().getText());
ecm.setPolymerisationTemperature(
assayEcmController
.getAssayEcm25DPanel()
.getPolymerizationTemperatureTextField()
.getText());
ecm.setPolymerisationPh(
assayEcmController
.getPolymerisationPhBindingList()
.get(
assayEcmController
.getAssayEcm25DPanel()
.getPolymerizationPhComboBox()
.getSelectedIndex()));
break;
}
newCondition.setAssay(assay);
newCondition.setEcm(ecm);
// create new treatment with same parameters as ones from previous condition
List<Treatment> treatmentList = new ArrayList<>();
ObservableList<Treatment> treatmentBindingList = treatmentsController.getTreatmentBindingList();
for (Treatment treatment : treatmentBindingList) {
Double concentration = treatment.getConcentration();
String concentrationUnit = treatment.getConcentrationUnit();
String timing = treatment.getTiming();
String drugSolvent = treatment.getDrugSolvent();
Double drugSolventConcentration = treatment.getDrugSolventConcentration();
TreatmentType treatmentType = treatment.getTreatmentType();
Treatment newTreatment =
new Treatment(
concentration,
concentrationUnit,
timing,
drugSolvent,
drugSolventConcentration,
treatmentType);
newTreatment.setPlateCondition(newCondition);
treatmentList.add(newTreatment);
}
newCondition.setTreatmentList(treatmentList);
// set an empty collection of wells (wells are not recalled from previous condition)
List<Well> wellList = new ArrayList<>();
newCondition.setWellList(wellList);
}
/** Initialize ConditionsPanel components */
private void initConditionsPanel() {
// set selected matrix dimension to "2D"
setupConditionsPanel.getEcmDimensionComboBox().setSelectedIndex(0);
setupConditionsPanel.getjTabbedPane1().setEnabled(false);
// set current and previous conditions indexes
conditionIndex = 0;
previousConditionIndex = -1;
conditionsPanel.getAddButton().setEnabled(false);
// init conditionJList (create new empty list) (conditions are NOT retrieved from DB)
plateConditionBindingList =
ObservableCollections.observableList(new ArrayList<PlateCondition>());
// autobind cell line
// autobind seeding density
Binding binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.seedingDensity"),
setupConditionsPanel.getSeedingDensityTextField(),
BeanProperty.create("text"),
"seedingdensitybinding");
bindingGroup.addBinding(binding);
// autobind seeding time
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.seedingTime"),
setupConditionsPanel.getSeedingTimeTextField(),
BeanProperty.create("text"),
"seedingtimebinding");
bindingGroup.addBinding(binding);
// autobind growth medium
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.growthMedium"),
setupConditionsPanel.getGrowthMediumComboBox(),
BeanProperty.create("selectedItem"),
"growthmediumbinding");
bindingGroup.addBinding(binding);
// autobind serum
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.serum"),
setupConditionsPanel.getSerumComboBox(),
BeanProperty.create("selectedItem"),
"celllineserumbinding");
bindingGroup.addBinding(binding);
// autobind serum concentration
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.serumConcentration"),
setupConditionsPanel.getSerumConcentrationTextField(),
BeanProperty.create("text"),
"celllineserumconcentrationbinding");
bindingGroup.addBinding(binding);
// autobind cell line type
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.cellLine.cellLineType"),
setupConditionsPanel.getCellLineComboBox(),
BeanProperty.create("selectedItem"),
"celllinetypebinding");
bindingGroup.addBinding(binding);
// autobind assay medium
// autobind medium
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.assayMedium.medium"),
treatmentsController.getTreatmentsPanel().getAssayMediumComboBox(),
BeanProperty.create("selectedItem"),
"assaymediumbinding");
bindingGroup.addBinding(binding);
// autobind serum
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.assayMedium.serum"),
treatmentsController.getTreatmentsPanel().getSerumComboBox(),
BeanProperty.create("selectedItem"),
"assayserumbinding");
bindingGroup.addBinding(binding);
// autobind serum concentration
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.assayMedium.serumConcentration"),
treatmentsController.getTreatmentsPanel().getSerumConcentrationTextField(),
BeanProperty.create("text"),
"assayserumconcentrationbinding");
bindingGroup.addBinding(binding);
// autobind volume
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.assayMedium.volume"),
treatmentsController.getTreatmentsPanel().getMediumVolumeTextField(),
BeanProperty.create("text"),
"assayvolumebinding");
bindingGroup.addBinding(binding);
// autobind matrix dimension
binding =
Bindings.createAutoBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
conditionsPanel.getConditionsJList(),
BeanProperty.create("selectedElement.matrixDimension"),
setupConditionsPanel.getEcmDimensionComboBox(),
BeanProperty.create("selectedItem"),
"matrixdimensionbinding");
bindingGroup.addBinding(binding);
bindingGroup.bind();
// init conditionListBinding
JListBinding conditionListBinding =
SwingBindings.createJListBinding(
AutoBinding.UpdateStrategy.READ_WRITE,
plateConditionBindingList,
conditionsPanel.getConditionsJList());
bindingGroup.addBinding(conditionListBinding);
bindingGroup.bind();
// create and init the first condition (Condition 1)
PlateCondition firstCondition = createFirstCondition();
// add Condition 1 to the list
plateConditionBindingList.add(firstCondition);
// set cell renderer for conditionJList
conditionsPanel.getConditionsJList().setCellRenderer(new ConditionsSetupListRenderer());
/** add mouse listeners */
// if Condition validation is OK, update previous condition and user input fields
conditionsPanel
.getConditionsJList()
.getSelectionModel()
.addListSelectionListener(
new ListSelectionListener() {
@Override
public void valueChanged(ListSelectionEvent e) {
if (!e.getValueIsAdjusting()) {
conditionsPanel.getAddButton().setEnabled(true);
int selectedIndex = conditionsPanel.getConditionsJList().getSelectedIndex();
if (selectedIndex != -1) {
// add mouse listener and enable tabbed pane on the right (only once, for
// Condition 1)
if (selectedIndex == 0) {
setupExperimentController.addMouseListener();
setupConditionsPanel.getjTabbedPane1().setEnabled(true);
}
if (previousConditionIndex < plateConditionBindingList.size()
&& previousConditionIndex != -1) {
// check if validation of condition is OK
if (setupExperimentController.validateCondition(
plateConditionBindingList.get(previousConditionIndex))) {
// update fields of previous condition
updateCondition(previousConditionIndex);
// update and reset fields for the assay-ecm panel
assayEcmController.updateAssayEcmInputFields(
plateConditionBindingList.get(selectedIndex));
// assayEcmPanelController.resetAssayEcmInputFields(plateConditionBindingList.get(locationToIndex));
// keep source and destination lists sync: show actual treatment collection
treatmentsController.updateLists(
plateConditionBindingList.get(selectedIndex));
}
}
previousConditionIndex = selectedIndex;
}
}
}
});
// add an empty list of rectangles for Condition 1
setupExperimentController.onNewConditionAdded(firstCondition);
// disable the Remove Button
conditionsPanel.getRemoveButton().setEnabled(false);
/** add action listeners */
// add a new Condition to the List
// each new Condition is init through values selected from the previously created one!
conditionsPanel
.getAddButton()
.addActionListener(
new ActionListener() {
@Override
public void actionPerformed(ActionEvent e) {
// create and init a new Condition
PlateCondition newCondition = new PlateCondition();
initNewCondition(newCondition);
// add the new Condition to the list
plateConditionBindingList.add(newCondition);
// add a new empty list of rectangles for the just added Condition
setupExperimentController.onNewConditionAdded(newCondition);
// after a new condition is added enable the remove button
if (!conditionsPanel.getRemoveButton().isEnabled()) {
conditionsPanel.getRemoveButton().setEnabled(true);
}
}
});
// remove a Condition from the list
conditionsPanel
.getRemoveButton()
.addActionListener(
new ActionListener() {
@Override
public void actionPerformed(ActionEvent e) {
if (conditionsPanel.getConditionsJList().getSelectedValue() != null) {
// empty the list of rectangles for this Condition and reset to null the Condition
// of the associated wells
setupExperimentController.onConditionToRemove(
(PlateCondition) (conditionsPanel.getConditionsJList().getSelectedValue()));
// remove the Condition from the list
int selectedIndex = conditionsPanel.getConditionsJList().getSelectedIndex();
PlateCondition conditionToRemove = plateConditionBindingList.get(selectedIndex);
// if the condition to remove is the last one, decrease previous condition index
if (plateConditionBindingList.indexOf(conditionToRemove)
== plateConditionBindingList.size() - 1) {
previousConditionIndex = plateConditionBindingList.size() - 2;
}
// remove condition from the list
plateConditionBindingList.remove(conditionToRemove);
// select first condition after removing
conditionsPanel.getConditionsJList().setSelectedIndex(0);
// if there's only one condition left, disable again the remove button
if (plateConditionBindingList.size() == 1) {
conditionsPanel.getRemoveButton().setEnabled(false);
}
}
}
});
}