void showDialog() { int width = imp.getWidth(); int height = imp.getHeight(); Calibration cal = imp.getCalibration(); int places; if (cal.scaled()) { pixelWidth = cal.pixelWidth; pixelHeight = cal.pixelHeight; units = cal.getUnits(); places = 2; } else { pixelWidth = 1.0; pixelHeight = 1.0; units = "pixels"; places = 0; } if (areaPerPoint == 0.0) areaPerPoint = (width * cal.pixelWidth * height * cal.pixelHeight) / 81.0; // default to 9x9 grid ImageWindow win = imp.getWindow(); GenericDialog gd = new GenericDialog("Grid..."); gd.addChoice("Grid Type:", types, type); gd.addNumericField("Area per Point:", areaPerPoint, places, 6, units + "^2"); gd.addChoice("Color:", colors, color); gd.addCheckbox("Random Offset", randomOffset); gd.addDialogListener(this); gd.showDialog(); if (gd.wasCanceled()) showGrid(null); }
void scale(ImageProcessor ip) { if (newWindow) { Rectangle r = ip.getRoi(); ImagePlus imp2 = imp.createImagePlus(); imp2.setProcessor(title, ip.resize(newWidth, newHeight)); Calibration cal = imp2.getCalibration(); if (cal.scaled()) { cal.pixelWidth *= 1.0 / xscale; cal.pixelHeight *= 1.0 / yscale; } imp2.show(); imp.trimProcessor(); imp2.trimProcessor(); imp2.changes = true; } else { if (processStack && imp.getStackSize() > 1) { Undo.reset(); StackProcessor sp = new StackProcessor(imp.getStack(), ip); sp.scale(xscale, yscale, bgValue); } else { ip.snapshot(); Undo.setup(Undo.FILTER, imp); ip.setSnapshotCopyMode(true); ip.scale(xscale, yscale); ip.setSnapshotCopyMode(false); } imp.killRoi(); imp.updateAndDraw(); imp.changes = true; } }
void createNewStack(ImagePlus imp, ImageProcessor ip) { int nSlices = imp.getStackSize(); int w = imp.getWidth(), h = imp.getHeight(); ImagePlus imp2 = imp.createImagePlus(); Rectangle r = ip.getRoi(); boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight(); ImageStack stack1 = imp.getStack(); ImageStack stack2 = new ImageStack(newWidth, newHeight); ImageProcessor ip1, ip2; int method = interpolationMethod; if (w == 1 || h == 1) method = ImageProcessor.NONE; for (int i = 1; i <= nSlices; i++) { IJ.showStatus("Scale: " + i + "/" + nSlices); ip1 = stack1.getProcessor(i); String label = stack1.getSliceLabel(i); if (crop) { ip1.setRoi(r); ip1 = ip1.crop(); } ip1.setInterpolationMethod(method); ip2 = ip1.resize(newWidth, newHeight, averageWhenDownsizing); if (ip2 != null) stack2.addSlice(label, ip2); IJ.showProgress(i, nSlices); } imp2.setStack(title, stack2); Calibration cal = imp2.getCalibration(); if (cal.scaled()) { cal.pixelWidth *= 1.0 / xscale; cal.pixelHeight *= 1.0 / yscale; } IJ.showProgress(1.0); int[] dim = imp.getDimensions(); imp2.setDimensions(dim[2], dim[3], dim[4]); if (imp.isComposite()) { imp2 = new CompositeImage(imp2, ((CompositeImage) imp).getMode()); ((CompositeImage) imp2).copyLuts(imp); } if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true); if (newDepth > 0 && newDepth != oldDepth) imp2 = (new Resizer()).zScale(imp2, newDepth, interpolationMethod); if (imp2 != null) { imp2.show(); imp2.changes = true; } }
void checkForCalibrationConflict(ImagePlus imp, Calibration cal) { Calibration gcal = imp.getGlobalCalibration(); if (gcal == null || !showConflictMessage || IJ.isMacro()) return; if (cal.pixelWidth == gcal.pixelWidth && cal.getUnit().equals(gcal.getUnit())) return; GenericDialog gd = new GenericDialog(imp.getTitle()); gd.addMessage("The calibration of this image conflicts\nwith the current global calibration."); gd.addCheckbox("Disable_Global Calibration", true); gd.addCheckbox("Disable_these Messages", false); gd.showDialog(); if (gd.wasCanceled()) return; boolean disable = gd.getNextBoolean(); if (disable) { imp.setGlobalCalibration(null); imp.setCalibration(cal); WindowManager.repaintImageWindows(); } boolean dontShow = gd.getNextBoolean(); if (dontShow) showConflictMessage = false; }
void createNewStack(ImagePlus imp, ImageProcessor ip) { Rectangle r = ip.getRoi(); boolean crop = r.width != imp.getWidth() || r.height != imp.getHeight(); int nSlices = imp.getStackSize(); ImageStack stack1 = imp.getStack(); ImageStack stack2 = new ImageStack(newWidth, newHeight); ImageProcessor ip1, ip2; boolean interp = interpolate; if (imp.getWidth() == 1 || imp.getHeight() == 1) interp = false; for (int i = 1; i <= nSlices; i++) { IJ.showStatus("Scale: " + i + "/" + nSlices); ip1 = stack1.getProcessor(i); String label = stack1.getSliceLabel(i); if (crop) { ip1.setRoi(r); ip1 = ip1.crop(); } ip1.setInterpolate(interp); ip2 = ip1.resize(newWidth, newHeight); if (ip2 != null) stack2.addSlice(label, ip2); IJ.showProgress(i, nSlices); } ImagePlus imp2 = imp.createImagePlus(); imp2.setStack(title, stack2); Calibration cal = imp2.getCalibration(); if (cal.scaled()) { cal.pixelWidth *= 1.0 / xscale; cal.pixelHeight *= 1.0 / yscale; } int[] dim = imp.getDimensions(); imp2.setDimensions(dim[2], dim[3], dim[4]); IJ.showProgress(1.0); if (imp.isComposite()) { imp2 = new CompositeImage(imp2, 0); ((CompositeImage) imp2).copyLuts(imp); } if (imp.isHyperStack()) imp2.setOpenAsHyperStack(true); imp2.show(); imp2.changes = true; }
void createMask(ImagePlus imp) { Roi roi = imp.getRoi(); boolean useInvertingLut = Prefs.useInvertingLut; Prefs.useInvertingLut = false; boolean selectAll = roi != null && roi.getType() == Roi.RECTANGLE && roi.getBounds().width == imp.getWidth() && roi.getBounds().height == imp.getHeight() && imp.isThreshold(); if (roi == null || !(roi.isArea() || roi.getType() == Roi.POINT) || selectAll) { createMaskFromThreshold(imp); Prefs.useInvertingLut = useInvertingLut; return; } ImagePlus maskImp = null; Frame frame = WindowManager.getFrame("Mask"); if (frame != null && (frame instanceof ImageWindow)) maskImp = ((ImageWindow) frame).getImagePlus(); if (maskImp == null) { ImageProcessor ip = new ByteProcessor(imp.getWidth(), imp.getHeight()); if (!Prefs.blackBackground) ip.invertLut(); maskImp = new ImagePlus("Mask", ip); maskImp.show(); } ImageProcessor ip = maskImp.getProcessor(); ip.setRoi(roi); ip.setValue(255); ip.fill(ip.getMask()); Calibration cal = imp.getCalibration(); if (cal.scaled()) { Calibration cal2 = maskImp.getCalibration(); cal2.pixelWidth = cal.pixelWidth; cal2.pixelHeight = cal.pixelHeight; cal2.setUnit(cal.getUnit()); } maskImp.updateAndRepaintWindow(); Prefs.useInvertingLut = useInvertingLut; }
void setCalibration(ImagePlus imp) { if (fi.fileType == FileInfo.GRAY16_SIGNED) { if (IJ.debugMode) IJ.log("16-bit signed"); double[] coeff = new double[2]; coeff[0] = -32768.0; coeff[1] = 1.0; imp.getLocalCalibration().setFunction(Calibration.STRAIGHT_LINE, coeff, "gray value"); } Properties props = decodeDescriptionString(fi); Calibration cal = imp.getCalibration(); boolean calibrated = false; if (fi.pixelWidth > 0.0 && fi.unit != null) { cal.pixelWidth = fi.pixelWidth; cal.pixelHeight = fi.pixelHeight; cal.pixelDepth = fi.pixelDepth; cal.setUnit(fi.unit); calibrated = true; } if (fi.valueUnit != null) { int f = fi.calibrationFunction; if ((f >= Calibration.STRAIGHT_LINE && f <= Calibration.RODBARD2 && fi.coefficients != null) || f == Calibration.UNCALIBRATED_OD) { boolean zeroClip = props != null && props.getProperty("zeroclip", "false").equals("true"); cal.setFunction(f, fi.coefficients, fi.valueUnit, zeroClip); calibrated = true; } } if (calibrated) checkForCalibrationConflict(imp, cal); if (fi.frameInterval != 0.0) cal.frameInterval = fi.frameInterval; if (props == null) return; cal.xOrigin = getDouble(props, "xorigin"); cal.yOrigin = getDouble(props, "yorigin"); cal.zOrigin = getDouble(props, "zorigin"); cal.info = props.getProperty("info"); cal.fps = getDouble(props, "fps"); cal.loop = getBoolean(props, "loop"); cal.frameInterval = getDouble(props, "finterval"); cal.setTimeUnit(props.getProperty("tunit", "sec")); double displayMin = getDouble(props, "min"); double displayMax = getDouble(props, "max"); if (!(displayMin == 0.0 && displayMax == 0.0)) { int type = imp.getType(); ImageProcessor ip = imp.getProcessor(); if (type == ImagePlus.GRAY8 || type == ImagePlus.COLOR_256) ip.setMinAndMax(displayMin, displayMax); else if (type == ImagePlus.GRAY16 || type == ImagePlus.GRAY32) { if (ip.getMin() != displayMin || ip.getMax() != displayMax) ip.setMinAndMax(displayMin, displayMax); } } int stackSize = imp.getStackSize(); if (stackSize > 1) { int channels = (int) getDouble(props, "channels"); int slices = (int) getDouble(props, "slices"); int frames = (int) getDouble(props, "frames"); if (channels == 0) channels = 1; if (slices == 0) slices = 1; if (frames == 0) frames = 1; // IJ.log("setCalibration: "+channels+" "+slices+" "+frames); if (channels * slices * frames == stackSize) { imp.setDimensions(channels, slices, frames); if (getBoolean(props, "hyperstack")) imp.setOpenAsHyperStack(true); } } }
private void makeBand(ImagePlus imp) { Roi roi = imp.getRoi(); if (roi == null) { noRoi("Make Band"); return; } if (!roi.isArea()) { IJ.error("Make Band", "Area selection required"); return; } Calibration cal = imp.getCalibration(); double pixels = bandSize; double size = pixels * cal.pixelWidth; int decimalPlaces = 0; if ((int) size != size) decimalPlaces = 2; GenericDialog gd = new GenericDialog("Make Band"); gd.addNumericField("Band Size:", size, decimalPlaces, 4, cal.getUnits()); gd.showDialog(); if (gd.wasCanceled()) return; size = gd.getNextNumber(); if (Double.isNaN(size)) { IJ.error("Make Band", "invalid number"); return; } int n = (int) Math.round(size / cal.pixelWidth); if (n > 255) { IJ.error("Make Band", "Cannot make bands wider that 255 pixels"); return; } int width = imp.getWidth(); int height = imp.getHeight(); Rectangle r = roi.getBounds(); ImageProcessor ip = roi.getMask(); if (ip == null) { ip = new ByteProcessor(r.width, r.height); ip.invert(); } ImageProcessor mask = new ByteProcessor(width, height); mask.insert(ip, r.x, r.y); ImagePlus edm = new ImagePlus("mask", mask); boolean saveBlackBackground = Prefs.blackBackground; Prefs.blackBackground = false; IJ.run(edm, "Distance Map", ""); Prefs.blackBackground = saveBlackBackground; ip = edm.getProcessor(); ip.setThreshold(0, n, ImageProcessor.NO_LUT_UPDATE); int xx = -1, yy = -1; for (int x = r.x; x < r.x + r.width; x++) { for (int y = r.y; y < r.y + r.height; y++) { if (ip.getPixel(x, y) < n) { xx = x; yy = y; break; } } if (xx >= 0 || yy >= 0) break; } int count = IJ.doWand(edm, xx, yy, 0, null); if (count <= 0) { IJ.error("Make Band", "Unable to make band"); return; } ShapeRoi roi2 = new ShapeRoi(edm.getRoi()); if (!(roi instanceof ShapeRoi)) roi = new ShapeRoi(roi); ShapeRoi roi1 = (ShapeRoi) roi; roi2 = roi2.not(roi1); imp.setRoi(roi2); bandSize = n; }
String getInfo(ImagePlus imp, ImageProcessor ip) { String s = new String("\n"); s += "Title: " + imp.getTitle() + "\n"; Calibration cal = imp.getCalibration(); int stackSize = imp.getStackSize(); int channels = imp.getNChannels(); int slices = imp.getNSlices(); int frames = imp.getNFrames(); int digits = imp.getBitDepth() == 32 ? 4 : 0; if (cal.scaled()) { String unit = cal.getUnit(); String units = cal.getUnits(); s += "Width: " + IJ.d2s(imp.getWidth() * cal.pixelWidth, 2) + " " + units + " (" + imp.getWidth() + ")\n"; s += "Height: " + IJ.d2s(imp.getHeight() * cal.pixelHeight, 2) + " " + units + " (" + imp.getHeight() + ")\n"; if (slices > 1) s += "Depth: " + IJ.d2s(slices * cal.pixelDepth, 2) + " " + units + " (" + slices + ")\n"; double xResolution = 1.0 / cal.pixelWidth; double yResolution = 1.0 / cal.pixelHeight; int places = Tools.getDecimalPlaces(xResolution, yResolution); if (xResolution == yResolution) s += "Resolution: " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n"; else { s += "X Resolution: " + IJ.d2s(xResolution, places) + " pixels per " + unit + "\n"; s += "Y Resolution: " + IJ.d2s(yResolution, places) + " pixels per " + unit + "\n"; } } else { s += "Width: " + imp.getWidth() + " pixels\n"; s += "Height: " + imp.getHeight() + " pixels\n"; if (stackSize > 1) s += "Depth: " + slices + " pixels\n"; } if (stackSize > 1) s += "Voxel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + "x" + d2s(cal.pixelDepth) + " " + cal.getUnit() + "\n"; else s += "Pixel size: " + d2s(cal.pixelWidth) + "x" + d2s(cal.pixelHeight) + " " + cal.getUnit() + "\n"; s += "ID: " + imp.getID() + "\n"; String zOrigin = stackSize > 1 || cal.zOrigin != 0.0 ? "," + d2s(cal.zOrigin) : ""; s += "Coordinate origin: " + d2s(cal.xOrigin) + "," + d2s(cal.yOrigin) + zOrigin + "\n"; int type = imp.getType(); switch (type) { case ImagePlus.GRAY8: s += "Bits per pixel: 8 "; String lut = "LUT"; if (imp.getProcessor().isColorLut()) lut = "color " + lut; else lut = "grayscale " + lut; if (imp.isInvertedLut()) lut = "inverting " + lut; s += "(" + lut + ")\n"; if (imp.getNChannels() > 1) s += displayRanges(imp); else s += "Display range: " + (int) ip.getMin() + "-" + (int) ip.getMax() + "\n"; break; case ImagePlus.GRAY16: case ImagePlus.GRAY32: if (type == ImagePlus.GRAY16) { String sign = cal.isSigned16Bit() ? "signed" : "unsigned"; s += "Bits per pixel: 16 (" + sign + ")\n"; } else s += "Bits per pixel: 32 (float)\n"; if (imp.getNChannels() > 1) s += displayRanges(imp); else { s += "Display range: "; double min = ip.getMin(); double max = ip.getMax(); if (cal.calibrated()) { min = cal.getCValue((int) min); max = cal.getCValue((int) max); } s += IJ.d2s(min, digits) + " - " + IJ.d2s(max, digits) + "\n"; } break; case ImagePlus.COLOR_256: s += "Bits per pixel: 8 (color LUT)\n"; break; case ImagePlus.COLOR_RGB: s += "Bits per pixel: 32 (RGB)\n"; break; } double interval = cal.frameInterval; double fps = cal.fps; if (stackSize > 1) { ImageStack stack = imp.getStack(); int slice = imp.getCurrentSlice(); String number = slice + "/" + stackSize; String label = stack.getShortSliceLabel(slice); if (label != null && label.length() > 0) label = " (" + label + ")"; else label = ""; if (interval > 0.0 || fps != 0.0) { s += "Frame: " + number + label + "\n"; if (fps != 0.0) { String sRate = Math.abs(fps - Math.round(fps)) < 0.00001 ? IJ.d2s(fps, 0) : IJ.d2s(fps, 5); s += "Frame rate: " + sRate + " fps\n"; } if (interval != 0.0) s += "Frame interval: " + ((int) interval == interval ? IJ.d2s(interval, 0) : IJ.d2s(interval, 5)) + " " + cal.getTimeUnit() + "\n"; } else s += "Image: " + number + label + "\n"; if (imp.isHyperStack()) { if (channels > 1) s += " Channel: " + imp.getChannel() + "/" + channels + "\n"; if (slices > 1) s += " Slice: " + imp.getSlice() + "/" + slices + "\n"; if (frames > 1) s += " Frame: " + imp.getFrame() + "/" + frames + "\n"; } if (imp.isComposite()) { if (!imp.isHyperStack() && channels > 1) s += " Channels: " + channels + "\n"; String mode = ((CompositeImage) imp).getModeAsString(); s += " Composite mode: \"" + mode + "\"\n"; } } if (ip.getMinThreshold() == ImageProcessor.NO_THRESHOLD) s += "No Threshold\n"; else { double lower = ip.getMinThreshold(); double upper = ip.getMaxThreshold(); int dp = digits; if (cal.calibrated()) { lower = cal.getCValue((int) lower); upper = cal.getCValue((int) upper); dp = cal.isSigned16Bit() ? 0 : 4; } s += "Threshold: " + IJ.d2s(lower, dp) + "-" + IJ.d2s(upper, dp) + "\n"; } ImageCanvas ic = imp.getCanvas(); double mag = ic != null ? ic.getMagnification() : 1.0; if (mag != 1.0) s += "Magnification: " + IJ.d2s(mag, 2) + "\n"; if (cal.calibrated()) { s += " \n"; int curveFit = cal.getFunction(); s += "Calibration Function: "; if (curveFit == Calibration.UNCALIBRATED_OD) s += "Uncalibrated OD\n"; else if (curveFit == Calibration.CUSTOM) s += "Custom lookup table\n"; else s += CurveFitter.fList[curveFit] + "\n"; double[] c = cal.getCoefficients(); if (c != null) { s += " a: " + IJ.d2s(c[0], 6) + "\n"; s += " b: " + IJ.d2s(c[1], 6) + "\n"; if (c.length >= 3) s += " c: " + IJ.d2s(c[2], 6) + "\n"; if (c.length >= 4) s += " c: " + IJ.d2s(c[3], 6) + "\n"; if (c.length >= 5) s += " c: " + IJ.d2s(c[4], 6) + "\n"; } s += " Unit: \"" + cal.getValueUnit() + "\"\n"; } else s += "Uncalibrated\n"; FileInfo fi = imp.getOriginalFileInfo(); if (fi != null) { if (fi.url != null && !fi.url.equals("")) s += "URL: " + fi.url + "\n"; else if (fi.directory != null && fi.fileName != null) s += "Path: " + fi.directory + fi.fileName + "\n"; } ImageWindow win = imp.getWindow(); if (win != null) { Point loc = win.getLocation(); Dimension screen = IJ.getScreenSize(); s += "Screen location: " + loc.x + "," + loc.y + " (" + screen.width + "x" + screen.height + ")\n"; } Overlay overlay = imp.getOverlay(); if (overlay != null) { String hidden = imp.getHideOverlay() ? " (hidden)" : " "; int n = overlay.size(); String elements = n == 1 ? " element" : " elements"; s += "Overlay: " + n + elements + (imp.getHideOverlay() ? " (hidden)" : "") + "\n"; } else s += "No Overlay\n"; Roi roi = imp.getRoi(); if (roi == null) { if (cal.calibrated()) s += " \n"; s += "No Selection\n"; } else if (roi instanceof EllipseRoi) { s += "\nElliptical Selection\n"; double[] p = ((EllipseRoi) roi).getParams(); double dx = p[2] - p[0]; double dy = p[3] - p[1]; double major = Math.sqrt(dx * dx + dy * dy); s += " Major: " + IJ.d2s(major, 2) + "\n"; s += " Minor: " + IJ.d2s(major * p[4], 2) + "\n"; s += " X1: " + IJ.d2s(p[0], 2) + "\n"; s += " Y1: " + IJ.d2s(p[1], 2) + "\n"; s += " X2: " + IJ.d2s(p[2], 2) + "\n"; s += " Y2: " + IJ.d2s(p[3], 2) + "\n"; s += " Aspect ratio: " + IJ.d2s(p[4], 2) + "\n"; } else { s += " \n"; s += roi.getTypeAsString() + " Selection"; String points = null; if (roi instanceof PointRoi) { int npoints = ((PolygonRoi) roi).getNCoordinates(); String suffix = npoints > 1 ? "s)" : ")"; points = " (" + npoints + " point" + suffix; } String name = roi.getName(); if (name != null) { s += " (\"" + name + "\")"; if (points != null) s += "\n " + points; } else if (points != null) s += points; s += "\n"; Rectangle r = roi.getBounds(); if (roi instanceof Line) { Line line = (Line) roi; s += " X1: " + IJ.d2s(line.x1d * cal.pixelWidth) + "\n"; s += " Y1: " + IJ.d2s(yy(line.y1d, imp) * cal.pixelHeight) + "\n"; s += " X2: " + IJ.d2s(line.x2d * cal.pixelWidth) + "\n"; s += " Y2: " + IJ.d2s(yy(line.y2d, imp) * cal.pixelHeight) + "\n"; } else if (cal.scaled()) { s += " X: " + IJ.d2s(cal.getX(r.x)) + " (" + r.x + ")\n"; s += " Y: " + IJ.d2s(cal.getY(r.y, imp.getHeight())) + " (" + r.y + ")\n"; s += " Width: " + IJ.d2s(r.width * cal.pixelWidth) + " (" + r.width + ")\n"; s += " Height: " + IJ.d2s(r.height * cal.pixelHeight) + " (" + r.height + ")\n"; } else { s += " X: " + r.x + "\n"; s += " Y: " + yy(r.y, imp) + "\n"; s += " Width: " + r.width + "\n"; s += " Height: " + r.height + "\n"; } } return s; }
/** Displays a modal options dialog. */ public boolean showDialog() { Calibration cal = imp != null ? imp.getCalibration() : (new Calibration()); double unitSquared = cal.pixelWidth * cal.pixelHeight; if (pixelUnits) unitSquared = 1.0; if (Macro.getOptions() != null) { boolean oldMacro = updateMacroOptions(); if (oldMacro) unitSquared = 1.0; staticMinSize = 0.0; staticMaxSize = DEFAULT_MAX_SIZE; staticMinCircularity = 0.0; staticMaxCircularity = 1.0; staticShowChoice = NOTHING; } GenericDialog gd = new GenericDialog("Analyze Particles"); minSize = staticMinSize; maxSize = staticMaxSize; minCircularity = staticMinCircularity; maxCircularity = staticMaxCircularity; showChoice = staticShowChoice; if (maxSize == 999999) maxSize = DEFAULT_MAX_SIZE; options = staticOptions; String unit = cal.getUnit(); boolean scaled = cal.scaled(); if (unit.equals("inch")) { unit = "pixel"; unitSquared = 1.0; scaled = false; pixelUnits = true; } String units = unit + "^2"; int places = 0; double cmin = minSize * unitSquared; if ((int) cmin != cmin) places = 2; double cmax = maxSize * unitSquared; if ((int) cmax != cmax && cmax != DEFAULT_MAX_SIZE) places = 2; String minStr = ResultsTable.d2s(cmin, places); if (minStr.indexOf("-") != -1) { for (int i = places; i <= 6; i++) { minStr = ResultsTable.d2s(cmin, i); if (minStr.indexOf("-") == -1) break; } } String maxStr = ResultsTable.d2s(cmax, places); if (maxStr.indexOf("-") != -1) { for (int i = places; i <= 6; i++) { maxStr = ResultsTable.d2s(cmax, i); if (maxStr.indexOf("-") == -1) break; } } if (scaled) gd.setInsets(5, 0, 0); gd.addStringField("Size (" + units + "):", minStr + "-" + maxStr, 12); if (scaled) { gd.setInsets(0, 40, 5); gd.addCheckbox("Pixel units", pixelUnits); } gd.addStringField("Circularity:", IJ.d2s(minCircularity) + "-" + IJ.d2s(maxCircularity), 12); gd.addChoice("Show:", showStrings, showStrings[showChoice]); String[] labels = new String[8]; boolean[] states = new boolean[8]; labels[0] = "Display results"; states[0] = (options & SHOW_RESULTS) != 0; labels[1] = "Exclude on edges"; states[1] = (options & EXCLUDE_EDGE_PARTICLES) != 0; labels[2] = "Clear results"; states[2] = (options & CLEAR_WORKSHEET) != 0; labels[3] = "Include holes"; states[3] = (options & INCLUDE_HOLES) != 0; labels[4] = "Summarize"; states[4] = (options & DISPLAY_SUMMARY) != 0; labels[5] = "Record starts"; states[5] = (options & RECORD_STARTS) != 0; labels[6] = "Add to Manager"; states[6] = (options & ADD_TO_MANAGER) != 0; labels[7] = "In_situ Show"; states[7] = (options & IN_SITU_SHOW) != 0; gd.addCheckboxGroup(4, 2, labels, states); gd.addHelp(IJ.URL + "/docs/menus/analyze.html#ap"); gd.showDialog(); if (gd.wasCanceled()) return false; String size = gd.getNextString(); // min-max size if (scaled) pixelUnits = gd.getNextBoolean(); if (pixelUnits) unitSquared = 1.0; else unitSquared = cal.pixelWidth * cal.pixelHeight; String[] minAndMax = Tools.split(size, " -"); double mins = gd.parseDouble(minAndMax[0]); double maxs = minAndMax.length == 2 ? gd.parseDouble(minAndMax[1]) : Double.NaN; minSize = Double.isNaN(mins) ? DEFAULT_MIN_SIZE : mins / unitSquared; maxSize = Double.isNaN(maxs) ? DEFAULT_MAX_SIZE : maxs / unitSquared; if (minSize < DEFAULT_MIN_SIZE) minSize = DEFAULT_MIN_SIZE; if (maxSize < minSize) maxSize = DEFAULT_MAX_SIZE; staticMinSize = minSize; staticMaxSize = maxSize; minAndMax = Tools.split(gd.getNextString(), " -"); // min-max circularity double minc = gd.parseDouble(minAndMax[0]); double maxc = minAndMax.length == 2 ? gd.parseDouble(minAndMax[1]) : Double.NaN; minCircularity = Double.isNaN(minc) ? 0.0 : minc; maxCircularity = Double.isNaN(maxc) ? 1.0 : maxc; if (minCircularity < 0.0 || minCircularity > 1.0) minCircularity = 0.0; if (maxCircularity < minCircularity || maxCircularity > 1.0) maxCircularity = 1.0; if (minCircularity == 1.0 && maxCircularity == 1.0) minCircularity = 0.0; staticMinCircularity = minCircularity; staticMaxCircularity = maxCircularity; if (gd.invalidNumber()) { IJ.error("Bins invalid."); canceled = true; return false; } showChoice = gd.getNextChoiceIndex(); staticShowChoice = showChoice; if (gd.getNextBoolean()) options |= SHOW_RESULTS; else options &= ~SHOW_RESULTS; if (gd.getNextBoolean()) options |= EXCLUDE_EDGE_PARTICLES; else options &= ~EXCLUDE_EDGE_PARTICLES; if (gd.getNextBoolean()) options |= CLEAR_WORKSHEET; else options &= ~CLEAR_WORKSHEET; if (gd.getNextBoolean()) options |= INCLUDE_HOLES; else options &= ~INCLUDE_HOLES; if (gd.getNextBoolean()) options |= DISPLAY_SUMMARY; else options &= ~DISPLAY_SUMMARY; if (gd.getNextBoolean()) options |= RECORD_STARTS; else options &= ~RECORD_STARTS; if (gd.getNextBoolean()) options |= ADD_TO_MANAGER; else options &= ~ADD_TO_MANAGER; if (gd.getNextBoolean()) options |= IN_SITU_SHOW; else options &= ~IN_SITU_SHOW; staticOptions = options; options |= SHOW_PROGRESS; if ((options & DISPLAY_SUMMARY) != 0) Analyzer.setMeasurements(Analyzer.getMeasurements() | AREA); return true; }