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Blobsplorer version 3.0 23/7/2014 GENERAL USAGE NOTES ——————————————————— - This application displays contigs based on GC content, coverage information and taxonomic annotations in the form of taxon-annotated GC-coverage plots (TAGC plots) while facilitating the filtering of contigs in the aid of creating a better assembly. - Filtering options include taxonomic rank, taxon, minimum coverage value, maximum E-Value and contig length. - The application only accepts input files that match the novel formatting found at https://github.com/blaxterlab/blobology/tree/master/dev, -Blobsplorer allows for the creation of several export files, including: SVG files of the TAGC plot, the coverage and GC content subplots and the legend; a new blobplot.txt containing only visible contigs; a history file of filtering steps; and two files containing included and excluded contig IDs for further processing respectively. -Taxonomic annotations are only as good as the resource from which they were gathered. Installation ——————————————————— Required: -Java 1.8 -JFreeChart 1.0.17 -JCommon 1.0.22 -Batik 1.7 -FXGraphics2d 1.0 Steps for Installation: 1)Download GitHub repository 2)Either use the build.xml script by typing ‘ant’ to build Blobsplorer 3.0 or use the precompiled version 3)Type java -jar Blobsplorer 3.0 to run the application Contact details Association: IEB, University of Edinburgh email: fridayjocelyn@gmail.com or jocelynfriday@yahoo.com
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